Mycobacterium phage Jolie1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; unclassified Bclasvirinae

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8EEL7|W8EEL7_9CAUD Crossover junction endodeoxyribonuclease RuvC OS=Mycobacterium phage Jolie1 OX=1463812 GN=Jolie1_05 PE=3 SV=1
MM1 pKa = 7.57DD2 pKa = 4.59TEE4 pKa = 4.36TDD6 pKa = 3.67LTFDD10 pKa = 5.24DD11 pKa = 4.62CLTWAEE17 pKa = 3.93WWDD20 pKa = 3.71EE21 pKa = 3.82FHH23 pKa = 7.79ANACCPNSSVAAARR37 pKa = 11.84LCGCGGSGEE46 pKa = 4.39LPSGVSRR53 pKa = 11.84LLIGDD58 pKa = 4.24PEE60 pKa = 4.01

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8EIV0|W8EIV0_9CAUD Uncharacterized protein OS=Mycobacterium phage Jolie1 OX=1463812 GN=Jolie1_089 PE=4 SV=1
MM1 pKa = 7.34TVTPPQPGSGKK12 pKa = 8.5SRR14 pKa = 11.84RR15 pKa = 11.84CNGHH19 pKa = 6.31KK20 pKa = 9.26DD21 pKa = 3.25TRR23 pKa = 11.84LLLEE27 pKa = 4.2AVEE30 pKa = 4.3AAGGEE35 pKa = 4.56CVPHH39 pKa = 6.31GRR41 pKa = 11.84TRR43 pKa = 11.84PGHH46 pKa = 5.03WKK48 pKa = 10.26VYY50 pKa = 10.64LDD52 pKa = 3.42GKK54 pKa = 9.78MIGGISGTPSDD65 pKa = 3.67HH66 pKa = 6.27RR67 pKa = 11.84TRR69 pKa = 11.84KK70 pKa = 9.87NDD72 pKa = 2.98IARR75 pKa = 11.84LRR77 pKa = 11.84RR78 pKa = 11.84NGLNITSKK86 pKa = 9.63GTYY89 pKa = 9.93DD90 pKa = 3.24GAAPQDD96 pKa = 3.67RR97 pKa = 11.84PP98 pKa = 3.54

Molecular weight: 10.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

22649

48

1992

231.1

24.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.513 ± 0.368

0.993 ± 0.144

6.42 ± 0.228

6.014 ± 0.291

2.552 ± 0.114

9.502 ± 0.56

1.753 ± 0.161

4.256 ± 0.173

2.693 ± 0.164

8.102 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.105

2.715 ± 0.172

6.482 ± 0.242

3.576 ± 0.153

6.769 ± 0.368

4.923 ± 0.181

7.02 ± 0.193

7.32 ± 0.198

2.0 ± 0.163

2.159 ± 0.132
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski