Euphorbia caput-medusae latent virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Capulavirus

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A166V367|A0A166V367_9GEMI Replication-associated protein OS=Euphorbia caput-medusae latent virus OX=1853865 PE=3 SV=1
MM1 pKa = 7.67PRR3 pKa = 11.84QPNNTFRR10 pKa = 11.84LQGKK14 pKa = 9.52SIFLTYY20 pKa = 9.22PKK22 pKa = 10.43CPLLPIFLIDD32 pKa = 4.25YY33 pKa = 9.3LYY35 pKa = 11.06QLLNNFNPTYY45 pKa = 11.13ARR47 pKa = 11.84VCTEE51 pKa = 3.51NHH53 pKa = 6.07QDD55 pKa = 3.87GEE57 pKa = 4.4PHH59 pKa = 6.41LHH61 pKa = 6.89CLVQMDD67 pKa = 4.29KK68 pKa = 11.07RR69 pKa = 11.84LNTTNQRR76 pKa = 11.84FFDD79 pKa = 3.55IKK81 pKa = 10.94DD82 pKa = 3.56PNGVATYY89 pKa = 10.18HH90 pKa = 6.71PNCQVPRR97 pKa = 11.84RR98 pKa = 11.84DD99 pKa = 3.84ADD101 pKa = 3.58VADD104 pKa = 4.81YY105 pKa = 10.27IAKK108 pKa = 10.16GGQFEE113 pKa = 4.22EE114 pKa = 4.62RR115 pKa = 11.84GILRR119 pKa = 11.84ASRR122 pKa = 11.84RR123 pKa = 11.84SPKK126 pKa = 9.01KK127 pKa = 9.02TRR129 pKa = 11.84DD130 pKa = 3.88TIWASILTEE139 pKa = 4.11STSKK143 pKa = 11.01SEE145 pKa = 3.58FLARR149 pKa = 11.84VQHH152 pKa = 5.3EE153 pKa = 4.35QPYY156 pKa = 9.72VWATQLRR163 pKa = 11.84NLEE166 pKa = 4.0YY167 pKa = 10.4AANSKK172 pKa = 9.35WPEE175 pKa = 4.4PITVFEE181 pKa = 4.18PRR183 pKa = 11.84FVNFPRR189 pKa = 11.84IPEE192 pKa = 5.26PIQQWAEE199 pKa = 4.05TNLFTVSAHH208 pKa = 7.37AIQLLGADD216 pKa = 4.3LTIEE220 pKa = 4.8DD221 pKa = 4.55IRR223 pKa = 11.84WAHH226 pKa = 6.91DD227 pKa = 3.31LTTEE231 pKa = 4.99FINDD235 pKa = 3.43EE236 pKa = 4.72LINNIDD242 pKa = 3.62EE243 pKa = 5.0PIIINDD249 pKa = 4.03SGPEE253 pKa = 3.87TT254 pKa = 3.76

Molecular weight: 29.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A166V367|A0A166V367_9GEMI Replication-associated protein OS=Euphorbia caput-medusae latent virus OX=1853865 PE=3 SV=1
MM1 pKa = 6.44VTTRR5 pKa = 11.84SGRR8 pKa = 11.84NYY10 pKa = 9.72QALVPTWARR19 pKa = 11.84KK20 pKa = 9.04KK21 pKa = 10.39RR22 pKa = 11.84RR23 pKa = 11.84STLARR28 pKa = 11.84STIVGPIRR36 pKa = 11.84RR37 pKa = 11.84PNYY40 pKa = 9.27QYY42 pKa = 9.28KK43 pKa = 8.84TRR45 pKa = 11.84YY46 pKa = 6.85TPHH49 pKa = 7.09RR50 pKa = 11.84PQHH53 pKa = 6.26KK54 pKa = 9.36IHH56 pKa = 6.57SLSQTRR62 pKa = 11.84VVSGSDD68 pKa = 2.91NGYY71 pKa = 8.16GWHH74 pKa = 6.08VSGVNIGSGFEE85 pKa = 4.42DD86 pKa = 3.1RR87 pKa = 11.84HH88 pKa = 6.39SDD90 pKa = 3.36KK91 pKa = 11.09IKK93 pKa = 10.51IINLKK98 pKa = 10.72FMMQLKK104 pKa = 9.56TSDD107 pKa = 3.56AGQQASCYY115 pKa = 8.71HH116 pKa = 5.92NLYY119 pKa = 9.58MFLVKK124 pKa = 10.56DD125 pKa = 3.84NSGGAQVPKK134 pKa = 10.28FNSIVMMDD142 pKa = 3.75NSNPATAEE150 pKa = 3.69VDD152 pKa = 3.19HH153 pKa = 7.16DD154 pKa = 4.29SKK156 pKa = 11.8DD157 pKa = 2.86RR158 pKa = 11.84FTIVRR163 pKa = 11.84RR164 pKa = 11.84WKK166 pKa = 10.53FSFKK170 pKa = 10.92GNSSKK175 pKa = 11.26NGTAYY180 pKa = 10.55DD181 pKa = 3.8CARR184 pKa = 11.84NLIDD188 pKa = 3.8FNRR191 pKa = 11.84NVKK194 pKa = 9.89INSVSEE200 pKa = 4.35FKK202 pKa = 10.93SATDD206 pKa = 3.46GSYY209 pKa = 11.84ANTQKK214 pKa = 10.84NAWVLYY220 pKa = 9.29IVPQIYY226 pKa = 9.25DD227 pKa = 3.75CTVDD231 pKa = 3.17GHH233 pKa = 6.23VKK235 pKa = 9.79IKK237 pKa = 10.45YY238 pKa = 9.48VSIVV242 pKa = 2.95

Molecular weight: 27.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

1276

68

331

182.3

21.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.016 ± 0.532

1.489 ± 0.286

4.467 ± 0.601

5.329 ± 0.971

5.094 ± 0.524

5.408 ± 0.851

2.273 ± 0.28

6.505 ± 0.74

5.094 ± 0.922

7.837 ± 1.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.803 ± 0.474

6.426 ± 0.554

6.426 ± 1.007

5.329 ± 0.482

6.818 ± 0.233

7.21 ± 1.501

6.583 ± 0.444

4.389 ± 0.788

1.959 ± 0.115

4.545 ± 0.605
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski