Thiomonas sp. X19

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiales genera incertae sedis; Thiomonas; unclassified Thiomonas

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4745 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A238DU23|A0A238DU23_9BURK Single-strand binding protein/Primosomal replication protein n OS=Thiomonas sp. X19 OX=1050370 GN=THIX_70036 PE=4 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84QTLLAAALAALPFLASPLALAQTLPPAPAPVPDD35 pKa = 4.16AQTTMRR41 pKa = 11.84TAATQNGSSAYY52 pKa = 9.33SGGMAYY58 pKa = 10.2FDD60 pKa = 3.32QQAYY64 pKa = 9.6QDD66 pKa = 4.07AANTAQDD73 pKa = 3.98IANQDD78 pKa = 3.17AANASSGGYY87 pKa = 9.73GVGLSGSTAWQDD99 pKa = 3.32AAAQAQGVANDD110 pKa = 3.56QQNLVDD116 pKa = 3.97AQVTVQQQGSSSQAGAYY133 pKa = 7.85STEE136 pKa = 4.34SAQAPVVAQNDD147 pKa = 3.09NSAAQTSASSGEE159 pKa = 4.27SASNASITQSTGVPQGQPTVTNNTAEE185 pKa = 4.11YY186 pKa = 9.27LAAGAATQAMMSGEE200 pKa = 4.09ATDD203 pKa = 5.93GYY205 pKa = 10.92QSNIDD210 pKa = 3.45AYY212 pKa = 9.96KK213 pKa = 10.7AALQAAAXILGAVAGGIAGSAAEE236 pKa = 4.23GAATRR241 pKa = 11.84EE242 pKa = 3.88QGLQITVRR250 pKa = 11.84LDD252 pKa = 3.32DD253 pKa = 4.24GQAIAVTQAADD264 pKa = 3.25VQLAVGQRR272 pKa = 11.84VEE274 pKa = 4.65IIGNLYY280 pKa = 9.64GGKK283 pKa = 10.13ARR285 pKa = 11.84VLPMGG290 pKa = 4.05

Molecular weight: 28.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A238DK27|A0A238DK27_9BURK Transcriptional regulator LysR family OS=Thiomonas sp. X19 OX=1050370 GN=THIX_110122 PE=3 SV=1
MM1 pKa = 7.15TRR3 pKa = 11.84TVTRR7 pKa = 11.84KK8 pKa = 9.62GVQARR13 pKa = 11.84LTSRR17 pKa = 11.84IRR19 pKa = 11.84NSSTMAPKK27 pKa = 10.07VATMRR32 pKa = 11.84LGRR35 pKa = 11.84MPPPGNKK42 pKa = 8.78PSRR45 pKa = 11.84RR46 pKa = 11.84KK47 pKa = 9.73RR48 pKa = 11.84KK49 pKa = 8.86PPRR52 pKa = 11.84KK53 pKa = 9.79APTTPTTKK61 pKa = 10.19SPRR64 pKa = 11.84RR65 pKa = 11.84PKK67 pKa = 10.04PLPLVIFPANQPAMMPMMRR86 pKa = 11.84KK87 pKa = 9.27PIKK90 pKa = 10.75LMVFPLGQVQLAAGKK105 pKa = 10.01FPGLIRR111 pKa = 11.84RR112 pKa = 11.84SLEE115 pKa = 3.6AVAVNRR121 pKa = 11.84KK122 pKa = 8.5HH123 pKa = 6.5DD124 pKa = 3.87HH125 pKa = 6.55SIVPATWLKK134 pKa = 10.65ARR136 pKa = 11.84SARR139 pKa = 11.84RR140 pKa = 11.84AA141 pKa = 3.23

Molecular weight: 15.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4745

0

4745

1333605

20

2926

281.1

30.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.294 ± 0.054

1.003 ± 0.015

5.021 ± 0.025

4.709 ± 0.033

3.323 ± 0.026

8.179 ± 0.038

2.567 ± 0.018

4.253 ± 0.03

2.999 ± 0.03

11.021 ± 0.053

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.482 ± 0.02

2.481 ± 0.024

5.602 ± 0.032

4.64 ± 0.028

7.214 ± 0.039

5.425 ± 0.028

5.033 ± 0.028

7.095 ± 0.029

1.565 ± 0.018

2.093 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski