Anisakis simplex (Herring worm)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Nematoda; Chromadorea; Rhabditida; Spirurina; Ascaridomorpha; Ascaridoidea; Anisakidae; Anisakis; Anisakis simplex complex

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P6QAN6|A0A3P6QAN6_ANISI Uncharacterized protein OS=Anisakis simplex OX=6269 GN=ASIM_LOCUS9442 PE=4 SV=1
MM1 pKa = 7.56FDD3 pKa = 3.41VASKK7 pKa = 10.48IYY9 pKa = 10.36VATDD13 pKa = 3.33SAPVDD18 pKa = 3.38MATYY22 pKa = 9.29EE23 pKa = 4.35LCCDD27 pKa = 4.33MIDD30 pKa = 3.56VTIDD34 pKa = 2.92ISAIYY39 pKa = 9.51GCKK42 pKa = 9.79DD43 pKa = 3.2DD44 pKa = 4.44SAVNAAFDD52 pKa = 3.88SQSQAVIHH60 pKa = 6.57LKK62 pKa = 9.38TDD64 pKa = 3.02QVLFLRR70 pKa = 11.84QVFPQLMDD78 pKa = 3.01II79 pKa = 4.73

Molecular weight: 8.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P6P1K6|A0A3P6P1K6_ANISI Uncharacterized protein OS=Anisakis simplex OX=6269 GN=ASIM_LOCUS7810 PE=4 SV=1
MM1 pKa = 7.66KK2 pKa = 8.98KK3 pKa = 8.65TKK5 pKa = 10.27RR6 pKa = 11.84KK7 pKa = 9.73VIRR10 pKa = 11.84LQQRR14 pKa = 11.84KK15 pKa = 4.69TTRR18 pKa = 11.84KK19 pKa = 9.72KK20 pKa = 9.55IRR22 pKa = 11.84KK23 pKa = 7.61KK24 pKa = 9.03SRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.11RR31 pKa = 11.84RR32 pKa = 11.84VLRR35 pKa = 11.84SHH37 pKa = 6.75NFTEE41 pKa = 5.08LSARR45 pKa = 11.84NLKK48 pKa = 10.14LL49 pKa = 3.4

Molecular weight: 6.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20779

0

20779

5639772

29

7829

271.4

30.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.903 ± 0.018

2.167 ± 0.015

5.596 ± 0.017

6.356 ± 0.027

4.106 ± 0.016

5.383 ± 0.027

2.418 ± 0.009

5.765 ± 0.016

5.474 ± 0.022

8.866 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.585 ± 0.01

4.848 ± 0.015

4.576 ± 0.021

4.3 ± 0.018

5.93 ± 0.017

8.816 ± 0.029

5.609 ± 0.017

6.204 ± 0.015

1.086 ± 0.006

2.972 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski