Clostridium thermobutyricum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3294 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|N9WKP5|N9WKP5_9CLOT YCII domain-containing protein OS=Clostridium thermobutyricum OX=29372 GN=HMPREF1092_00642 PE=3 SV=1
MM1 pKa = 7.48NIANIVEE8 pKa = 4.1MLEE11 pKa = 3.97EE12 pKa = 4.18NRR14 pKa = 11.84LSEE17 pKa = 4.03ITEE20 pKa = 4.1LLVDD24 pKa = 3.76EE25 pKa = 5.12EE26 pKa = 4.66KK27 pKa = 10.95CVLEE31 pKa = 4.05FFYY34 pKa = 11.11DD35 pKa = 3.98FDD37 pKa = 5.07RR38 pKa = 11.84DD39 pKa = 3.74EE40 pKa = 5.38IEE42 pKa = 4.11GAKK45 pKa = 10.2AYY47 pKa = 10.58ANEE50 pKa = 4.0EE51 pKa = 3.98SDD53 pKa = 4.57LEE55 pKa = 4.31EE56 pKa = 4.68EE57 pKa = 4.31SEE59 pKa = 4.19EE60 pKa = 4.15WRR62 pKa = 11.84TDD64 pKa = 3.21YY65 pKa = 10.99YY66 pKa = 10.44IPYY69 pKa = 9.77LLDD72 pKa = 3.24IAKK75 pKa = 10.55DD76 pKa = 3.73NIEE79 pKa = 4.65SIVEE83 pKa = 4.25EE84 pKa = 4.0ICEE87 pKa = 3.86EE88 pKa = 4.1LGIEE92 pKa = 4.52GKK94 pKa = 10.49AEE96 pKa = 3.96EE97 pKa = 4.88LDD99 pKa = 3.26IDD101 pKa = 5.28ANTLDD106 pKa = 3.93YY107 pKa = 11.19IKK109 pKa = 10.62FRR111 pKa = 11.84VAFCIEE117 pKa = 4.54DD118 pKa = 3.57YY119 pKa = 11.28EE120 pKa = 5.27GDD122 pKa = 3.91IEE124 pKa = 5.4SLLNDD129 pKa = 3.68YY130 pKa = 10.84LL131 pKa = 4.9

Molecular weight: 15.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|N9WD57|N9WD57_9CLOT Uncharacterized protein OS=Clostridium thermobutyricum OX=29372 GN=HMPREF1092_02135 PE=4 SV=1
MM1 pKa = 7.33ARR3 pKa = 11.84KK4 pKa = 9.97AMIEE8 pKa = 3.69KK9 pKa = 9.58WNRR12 pKa = 11.84EE13 pKa = 3.87PKK15 pKa = 10.1YY16 pKa = 9.45KK17 pKa = 9.78TRR19 pKa = 11.84AYY21 pKa = 8.2TRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 6.74SVLKK35 pKa = 10.88KK36 pKa = 10.24FGICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.88KK50 pKa = 10.17GQIPGCKK57 pKa = 8.74KK58 pKa = 10.74ASWW61 pKa = 3.34

Molecular weight: 7.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3294

0

3294

975620

24

2605

296.2

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.022 ± 0.042

1.119 ± 0.017

5.323 ± 0.033

7.879 ± 0.059

4.63 ± 0.039

6.463 ± 0.043

1.22 ± 0.015

10.497 ± 0.063

9.786 ± 0.055

9.163 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.021

6.791 ± 0.059

2.575 ± 0.027

2.049 ± 0.027

3.296 ± 0.03

6.156 ± 0.038

4.691 ± 0.037

6.162 ± 0.042

0.641 ± 0.014

4.047 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski