Amino acid dipepetide frequency for Brevibacillus phage Emery

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.239AlaAla: 5.239 ± 0.779
0.16AlaCys: 0.16 ± 0.087
3.207AlaAsp: 3.207 ± 0.44
5.613AlaGlu: 5.613 ± 0.713
2.994AlaPhe: 2.994 ± 0.397
4.811AlaGly: 4.811 ± 0.691
1.283AlaHis: 1.283 ± 0.338
5.078AlaIle: 5.078 ± 0.516
4.864AlaLys: 4.864 ± 0.403
6.789AlaLeu: 6.789 ± 0.787
1.711AlaMet: 1.711 ± 0.303
3.956AlaAsn: 3.956 ± 0.428
1.711AlaPro: 1.711 ± 0.317
1.978AlaGln: 1.978 ± 0.252
3.635AlaArg: 3.635 ± 0.458
3.742AlaSer: 3.742 ± 0.465
3.528AlaThr: 3.528 ± 0.502
5.773AlaVal: 5.773 ± 0.482
0.909AlaTrp: 0.909 ± 0.25
2.566AlaTyr: 2.566 ± 0.274
0.0AlaXaa: 0.0 ± 0.0
Cys
0.374CysAla: 0.374 ± 0.132
0.053CysCys: 0.053 ± 0.062
0.535CysAsp: 0.535 ± 0.17
0.481CysGlu: 0.481 ± 0.153
0.267CysPhe: 0.267 ± 0.11
0.641CysGly: 0.641 ± 0.178
0.16CysHis: 0.16 ± 0.091
0.695CysIle: 0.695 ± 0.235
0.855CysLys: 0.855 ± 0.234
1.069CysLeu: 1.069 ± 0.232
0.267CysMet: 0.267 ± 0.12
0.321CysAsn: 0.321 ± 0.125
0.107CysPro: 0.107 ± 0.083
0.267CysGln: 0.267 ± 0.113
0.588CysArg: 0.588 ± 0.189
0.481CysSer: 0.481 ± 0.162
0.374CysThr: 0.374 ± 0.116
0.321CysVal: 0.321 ± 0.122
0.053CysTrp: 0.053 ± 0.054
0.321CysTyr: 0.321 ± 0.127
0.0CysXaa: 0.0 ± 0.0
Asp
3.261AspAla: 3.261 ± 0.472
0.267AspCys: 0.267 ± 0.105
3.261AspAsp: 3.261 ± 0.389
3.902AspGlu: 3.902 ± 0.504
2.78AspPhe: 2.78 ± 0.463
3.582AspGly: 3.582 ± 0.388
1.176AspHis: 1.176 ± 0.288
3.849AspIle: 3.849 ± 0.613
3.688AspLys: 3.688 ± 0.395
5.025AspLeu: 5.025 ± 0.551
1.443AspMet: 1.443 ± 0.29
1.764AspAsn: 1.764 ± 0.287
2.245AspPro: 2.245 ± 0.321
2.459AspGln: 2.459 ± 0.311
3.314AspArg: 3.314 ± 0.346
3.849AspSer: 3.849 ± 0.424
2.673AspThr: 2.673 ± 0.428
3.688AspVal: 3.688 ± 0.511
1.336AspTrp: 1.336 ± 0.256
2.673AspTyr: 2.673 ± 0.389
0.0AspXaa: 0.0 ± 0.0
Glu
6.201GluAla: 6.201 ± 0.546
0.641GluCys: 0.641 ± 0.188
3.849GluAsp: 3.849 ± 0.396
6.308GluGlu: 6.308 ± 0.845
3.1GluPhe: 3.1 ± 0.455
4.063GluGly: 4.063 ± 0.558
1.283GluHis: 1.283 ± 0.212
4.918GluIle: 4.918 ± 0.572
6.789GluLys: 6.789 ± 0.677
6.949GluLeu: 6.949 ± 0.658
2.138GluMet: 2.138 ± 0.35
3.261GluAsn: 3.261 ± 0.412
1.924GluPro: 1.924 ± 0.351
3.475GluGln: 3.475 ± 0.489
4.383GluArg: 4.383 ± 0.497
3.795GluSer: 3.795 ± 0.453
3.368GluThr: 3.368 ± 0.362
4.758GluVal: 4.758 ± 0.486
1.016GluTrp: 1.016 ± 0.224
2.673GluTyr: 2.673 ± 0.361
0.0GluXaa: 0.0 ± 0.0
Phe
2.78PheAla: 2.78 ± 0.495
0.802PheCys: 0.802 ± 0.232
3.795PheAsp: 3.795 ± 0.498
2.299PheGlu: 2.299 ± 0.418
1.764PhePhe: 1.764 ± 0.301
2.994PheGly: 2.994 ± 0.444
0.641PheHis: 0.641 ± 0.153
2.833PheIle: 2.833 ± 0.4
2.138PheLys: 2.138 ± 0.296
3.688PheLeu: 3.688 ± 0.484
1.069PheMet: 1.069 ± 0.216
2.085PheAsn: 2.085 ± 0.296
1.336PhePro: 1.336 ± 0.239
1.229PheGln: 1.229 ± 0.243
1.497PheArg: 1.497 ± 0.291
3.368PheSer: 3.368 ± 0.341
2.299PheThr: 2.299 ± 0.407
2.192PheVal: 2.192 ± 0.335
0.855PheTrp: 0.855 ± 0.22
1.016PheTyr: 1.016 ± 0.226
0.0PheXaa: 0.0 ± 0.0
Gly
3.742GlyAla: 3.742 ± 0.595
0.641GlyCys: 0.641 ± 0.196
3.154GlyAsp: 3.154 ± 0.486
3.849GlyGlu: 3.849 ± 0.547
3.1GlyPhe: 3.1 ± 0.382
4.544GlyGly: 4.544 ± 0.773
1.176GlyHis: 1.176 ± 0.234
4.223GlyIle: 4.223 ± 0.63
5.934GlyLys: 5.934 ± 0.554
5.078GlyLeu: 5.078 ± 0.527
1.497GlyMet: 1.497 ± 0.251
2.94GlyAsn: 2.94 ± 0.29
2.031GlyPro: 2.031 ± 0.303
2.192GlyGln: 2.192 ± 0.312
2.94GlyArg: 2.94 ± 0.443
4.17GlySer: 4.17 ± 0.5
4.544GlyThr: 4.544 ± 0.453
4.437GlyVal: 4.437 ± 0.459
0.962GlyTrp: 0.962 ± 0.233
2.352GlyTyr: 2.352 ± 0.343
0.0GlyXaa: 0.0 ± 0.0
His
1.123HisAla: 1.123 ± 0.259
0.214HisCys: 0.214 ± 0.095
1.016HisAsp: 1.016 ± 0.22
1.283HisGlu: 1.283 ± 0.236
0.535HisPhe: 0.535 ± 0.164
1.016HisGly: 1.016 ± 0.205
0.267HisHis: 0.267 ± 0.131
0.962HisIle: 0.962 ± 0.234
1.016HisLys: 1.016 ± 0.212
1.604HisLeu: 1.604 ± 0.326
0.641HisMet: 0.641 ± 0.186
0.695HisAsn: 0.695 ± 0.183
1.123HisPro: 1.123 ± 0.235
0.748HisGln: 0.748 ± 0.175
0.855HisArg: 0.855 ± 0.214
1.336HisSer: 1.336 ± 0.274
0.855HisThr: 0.855 ± 0.257
0.802HisVal: 0.802 ± 0.159
0.107HisTrp: 0.107 ± 0.113
0.641HisTyr: 0.641 ± 0.163
0.0HisXaa: 0.0 ± 0.0
Ile
5.827IleAla: 5.827 ± 0.594
0.321IleCys: 0.321 ± 0.12
4.17IleAsp: 4.17 ± 0.498
6.201IleGlu: 6.201 ± 0.503
1.657IlePhe: 1.657 ± 0.302
4.276IleGly: 4.276 ± 0.554
1.39IleHis: 1.39 ± 0.242
4.063IleIle: 4.063 ± 0.589
5.078IleLys: 5.078 ± 0.506
5.88IleLeu: 5.88 ± 0.704
1.604IleMet: 1.604 ± 0.38
3.528IleAsn: 3.528 ± 0.407
3.421IlePro: 3.421 ± 0.505
2.352IleGln: 2.352 ± 0.356
3.421IleArg: 3.421 ± 0.485
4.811IleSer: 4.811 ± 0.476
3.849IleThr: 3.849 ± 0.481
4.276IleVal: 4.276 ± 0.574
0.481IleTrp: 0.481 ± 0.179
1.55IleTyr: 1.55 ± 0.272
0.0IleXaa: 0.0 ± 0.0
Lys
5.613LysAla: 5.613 ± 0.598
0.748LysCys: 0.748 ± 0.171
3.368LysAsp: 3.368 ± 0.457
5.987LysGlu: 5.987 ± 0.514
2.512LysPhe: 2.512 ± 0.337
4.276LysGly: 4.276 ± 0.484
1.336LysHis: 1.336 ± 0.306
5.239LysIle: 5.239 ± 0.529
6.949LysLys: 6.949 ± 0.584
7.11LysLeu: 7.11 ± 0.55
2.352LysMet: 2.352 ± 0.319
3.421LysAsn: 3.421 ± 0.473
3.635LysPro: 3.635 ± 0.46
3.421LysGln: 3.421 ± 0.534
5.827LysArg: 5.827 ± 0.617
3.849LysSer: 3.849 ± 0.35
3.635LysThr: 3.635 ± 0.407
4.276LysVal: 4.276 ± 0.454
1.283LysTrp: 1.283 ± 0.304
2.512LysTyr: 2.512 ± 0.364
0.0LysXaa: 0.0 ± 0.0
Leu
6.415LeuAla: 6.415 ± 0.597
1.016LeuCys: 1.016 ± 0.238
5.078LeuAsp: 5.078 ± 0.496
8.286LeuGlu: 8.286 ± 0.681
3.742LeuPhe: 3.742 ± 0.417
6.361LeuGly: 6.361 ± 0.432
1.39LeuHis: 1.39 ± 0.275
5.773LeuIle: 5.773 ± 0.584
6.735LeuLys: 6.735 ± 0.73
8.018LeuLeu: 8.018 ± 0.801
2.566LeuMet: 2.566 ± 0.418
3.849LeuAsn: 3.849 ± 0.532
3.154LeuPro: 3.154 ± 0.323
4.17LeuGln: 4.17 ± 0.413
4.758LeuArg: 4.758 ± 0.412
6.789LeuSer: 6.789 ± 0.693
5.025LeuThr: 5.025 ± 0.478
5.613LeuVal: 5.613 ± 0.607
1.176LeuTrp: 1.176 ± 0.203
2.619LeuTyr: 2.619 ± 0.38
0.0LeuXaa: 0.0 ± 0.0
Met
2.352MetAla: 2.352 ± 0.311
0.107MetCys: 0.107 ± 0.074
1.604MetAsp: 1.604 ± 0.265
2.031MetGlu: 2.031 ± 0.307
1.069MetPhe: 1.069 ± 0.274
2.512MetGly: 2.512 ± 0.371
0.481MetHis: 0.481 ± 0.154
1.55MetIle: 1.55 ± 0.347
2.673MetLys: 2.673 ± 0.384
1.924MetLeu: 1.924 ± 0.337
0.481MetMet: 0.481 ± 0.145
1.069MetAsn: 1.069 ± 0.22
0.374MetPro: 0.374 ± 0.146
1.176MetGln: 1.176 ± 0.25
1.069MetArg: 1.069 ± 0.233
1.764MetSer: 1.764 ± 0.318
1.764MetThr: 1.764 ± 0.265
1.39MetVal: 1.39 ± 0.224
0.214MetTrp: 0.214 ± 0.098
0.695MetTyr: 0.695 ± 0.187
0.0MetXaa: 0.0 ± 0.0
Asn
3.368AsnAla: 3.368 ± 0.503
0.321AsnCys: 0.321 ± 0.139
2.673AsnAsp: 2.673 ± 0.271
2.619AsnGlu: 2.619 ± 0.441
1.443AsnPhe: 1.443 ± 0.254
2.994AsnGly: 2.994 ± 0.406
0.909AsnHis: 0.909 ± 0.209
2.192AsnIle: 2.192 ± 0.394
3.368AsnLys: 3.368 ± 0.451
4.063AsnLeu: 4.063 ± 0.456
1.176AsnMet: 1.176 ± 0.292
1.604AsnAsn: 1.604 ± 0.296
2.566AsnPro: 2.566 ± 0.419
1.657AsnGln: 1.657 ± 0.289
2.833AsnArg: 2.833 ± 0.35
2.619AsnSer: 2.619 ± 0.348
2.192AsnThr: 2.192 ± 0.267
2.994AsnVal: 2.994 ± 0.332
0.802AsnTrp: 0.802 ± 0.231
1.176AsnTyr: 1.176 ± 0.283
0.0AsnXaa: 0.0 ± 0.0
Pro
2.245ProAla: 2.245 ± 0.313
0.267ProCys: 0.267 ± 0.138
2.192ProAsp: 2.192 ± 0.349
3.1ProGlu: 3.1 ± 0.322
1.016ProPhe: 1.016 ± 0.229
2.245ProGly: 2.245 ± 0.352
0.909ProHis: 0.909 ± 0.196
3.582ProIle: 3.582 ± 0.491
2.512ProLys: 2.512 ± 0.389
2.94ProLeu: 2.94 ± 0.374
0.909ProMet: 0.909 ± 0.204
1.176ProAsn: 1.176 ± 0.227
1.39ProPro: 1.39 ± 0.314
1.39ProGln: 1.39 ± 0.281
1.764ProArg: 1.764 ± 0.341
2.78ProSer: 2.78 ± 0.356
1.978ProThr: 1.978 ± 0.345
2.833ProVal: 2.833 ± 0.349
0.428ProTrp: 0.428 ± 0.173
1.016ProTyr: 1.016 ± 0.218
0.0ProXaa: 0.0 ± 0.0
Gln
3.1GlnAla: 3.1 ± 0.381
0.267GlnCys: 0.267 ± 0.117
1.871GlnAsp: 1.871 ± 0.351
2.833GlnGlu: 2.833 ± 0.413
1.443GlnPhe: 1.443 ± 0.242
1.711GlnGly: 1.711 ± 0.296
0.481GlnHis: 0.481 ± 0.177
2.726GlnIle: 2.726 ± 0.433
3.795GlnLys: 3.795 ± 0.448
4.223GlnLeu: 4.223 ± 0.463
1.39GlnMet: 1.39 ± 0.288
1.764GlnAsn: 1.764 ± 0.259
1.283GlnPro: 1.283 ± 0.258
1.657GlnGln: 1.657 ± 0.268
2.085GlnArg: 2.085 ± 0.3
2.031GlnSer: 2.031 ± 0.313
1.711GlnThr: 1.711 ± 0.329
2.994GlnVal: 2.994 ± 0.48
0.695GlnTrp: 0.695 ± 0.207
1.978GlnTyr: 1.978 ± 0.333
0.0GlnXaa: 0.0 ± 0.0
Arg
3.635ArgAla: 3.635 ± 0.423
0.481ArgCys: 0.481 ± 0.184
3.1ArgAsp: 3.1 ± 0.314
3.368ArgGlu: 3.368 ± 0.435
2.512ArgPhe: 2.512 ± 0.296
2.726ArgGly: 2.726 ± 0.413
0.695ArgHis: 0.695 ± 0.168
4.063ArgIle: 4.063 ± 0.498
4.009ArgLys: 4.009 ± 0.439
6.094ArgLeu: 6.094 ± 0.571
1.497ArgMet: 1.497 ± 0.263
1.657ArgAsn: 1.657 ± 0.296
1.176ArgPro: 1.176 ± 0.222
2.031ArgGln: 2.031 ± 0.294
3.207ArgArg: 3.207 ± 0.453
3.368ArgSer: 3.368 ± 0.473
3.261ArgThr: 3.261 ± 0.428
3.421ArgVal: 3.421 ± 0.485
0.588ArgTrp: 0.588 ± 0.158
2.085ArgTyr: 2.085 ± 0.312
0.0ArgXaa: 0.0 ± 0.0
Ser
3.742SerAla: 3.742 ± 0.641
0.588SerCys: 0.588 ± 0.214
3.154SerAsp: 3.154 ± 0.456
4.49SerGlu: 4.49 ± 0.513
3.368SerPhe: 3.368 ± 0.41
4.651SerGly: 4.651 ± 0.463
1.283SerHis: 1.283 ± 0.307
4.758SerIle: 4.758 ± 0.423
5.239SerLys: 5.239 ± 0.448
5.987SerLeu: 5.987 ± 0.565
1.604SerMet: 1.604 ± 0.26
2.94SerAsn: 2.94 ± 0.448
2.459SerPro: 2.459 ± 0.419
2.459SerGln: 2.459 ± 0.301
2.512SerArg: 2.512 ± 0.36
4.971SerSer: 4.971 ± 0.786
4.009SerThr: 4.009 ± 0.488
3.742SerVal: 3.742 ± 0.443
0.588SerTrp: 0.588 ± 0.17
2.673SerTyr: 2.673 ± 0.439
0.0SerXaa: 0.0 ± 0.0
Thr
3.207ThrAla: 3.207 ± 0.444
0.428ThrCys: 0.428 ± 0.139
3.528ThrAsp: 3.528 ± 0.484
3.421ThrGlu: 3.421 ± 0.508
2.085ThrPhe: 2.085 ± 0.377
3.849ThrGly: 3.849 ± 0.498
0.588ThrHis: 0.588 ± 0.164
3.528ThrIle: 3.528 ± 0.453
3.582ThrLys: 3.582 ± 0.488
4.811ThrLeu: 4.811 ± 0.522
0.855ThrMet: 0.855 ± 0.209
2.887ThrAsn: 2.887 ± 0.419
2.726ThrPro: 2.726 ± 0.369
1.924ThrGln: 1.924 ± 0.289
2.619ThrArg: 2.619 ± 0.434
3.1ThrSer: 3.1 ± 0.414
3.154ThrThr: 3.154 ± 0.423
3.849ThrVal: 3.849 ± 0.56
0.855ThrTrp: 0.855 ± 0.193
2.619ThrTyr: 2.619 ± 0.392
0.0ThrXaa: 0.0 ± 0.0
Val
4.223ValAla: 4.223 ± 0.463
0.535ValCys: 0.535 ± 0.159
3.154ValAsp: 3.154 ± 0.367
5.292ValGlu: 5.292 ± 0.547
2.406ValPhe: 2.406 ± 0.403
3.582ValGly: 3.582 ± 0.43
1.016ValHis: 1.016 ± 0.22
5.025ValIle: 5.025 ± 0.624
4.49ValLys: 4.49 ± 0.448
6.094ValLeu: 6.094 ± 0.605
1.604ValMet: 1.604 ± 0.316
2.673ValAsn: 2.673 ± 0.399
2.566ValPro: 2.566 ± 0.328
3.154ValGln: 3.154 ± 0.415
2.94ValArg: 2.94 ± 0.372
4.971ValSer: 4.971 ± 0.426
3.421ValThr: 3.421 ± 0.535
4.597ValVal: 4.597 ± 0.528
1.016ValTrp: 1.016 ± 0.211
2.245ValTyr: 2.245 ± 0.402
0.0ValXaa: 0.0 ± 0.0
Trp
1.016TrpAla: 1.016 ± 0.242
0.053TrpCys: 0.053 ± 0.053
1.069TrpAsp: 1.069 ± 0.223
0.962TrpGlu: 0.962 ± 0.215
0.641TrpPhe: 0.641 ± 0.161
0.641TrpGly: 0.641 ± 0.174
0.107TrpHis: 0.107 ± 0.079
1.069TrpIle: 1.069 ± 0.256
0.962TrpLys: 0.962 ± 0.271
2.031TrpLeu: 2.031 ± 0.337
0.535TrpMet: 0.535 ± 0.128
0.695TrpAsn: 0.695 ± 0.185
0.267TrpPro: 0.267 ± 0.133
0.374TrpGln: 0.374 ± 0.131
0.641TrpArg: 0.641 ± 0.245
1.123TrpSer: 1.123 ± 0.319
0.374TrpThr: 0.374 ± 0.153
0.748TrpVal: 0.748 ± 0.268
0.107TrpTrp: 0.107 ± 0.074
0.214TrpTyr: 0.214 ± 0.105
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.085TyrAla: 2.085 ± 0.319
0.321TyrCys: 0.321 ± 0.135
2.459TyrAsp: 2.459 ± 0.356
2.566TyrGlu: 2.566 ± 0.417
2.245TyrPhe: 2.245 ± 0.319
2.031TyrGly: 2.031 ± 0.358
0.267TyrHis: 0.267 ± 0.114
2.031TyrIle: 2.031 ± 0.404
2.673TyrLys: 2.673 ± 0.44
3.154TyrLeu: 3.154 ± 0.431
0.909TyrMet: 0.909 ± 0.187
1.283TyrAsn: 1.283 ± 0.304
1.176TyrPro: 1.176 ± 0.26
1.924TyrGln: 1.924 ± 0.329
2.085TyrArg: 2.085 ± 0.331
2.352TyrSer: 2.352 ± 0.357
1.604TyrThr: 1.604 ± 0.266
2.299TyrVal: 2.299 ± 0.337
0.16TyrTrp: 0.16 ± 0.068
1.39TyrTyr: 1.39 ± 0.333
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 102 proteins (18708 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski