Chryseobacterium sp. Leaf201

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Weeksellaceae; Chryseobacterium group; Chryseobacterium; unclassified Chryseobacterium

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3820 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q4ENB9|A0A0Q4ENB9_9FLAO Uncharacterized protein OS=Chryseobacterium sp. Leaf201 OX=1735672 GN=ASE55_08850 PE=4 SV=1
MM1 pKa = 7.74LKK3 pKa = 9.91MNEE6 pKa = 3.71NWMQKK11 pKa = 9.14YY12 pKa = 9.66EE13 pKa = 4.15EE14 pKa = 4.26VKK16 pKa = 10.75DD17 pKa = 3.64ILVCPTDD24 pKa = 3.5LEE26 pKa = 4.98TYY28 pKa = 7.59FTSNEE33 pKa = 3.65IAGQQLEE40 pKa = 4.26TMEE43 pKa = 4.76IGNVSLPSGKK53 pKa = 10.1VVVRR57 pKa = 11.84DD58 pKa = 3.5PLVALNANQSPYY70 pKa = 10.36FIEE73 pKa = 4.32APKK76 pKa = 11.11GNFPVTIAVVKK87 pKa = 10.91SEE89 pKa = 4.12DD90 pKa = 3.02WGDD93 pKa = 3.2RR94 pKa = 11.84YY95 pKa = 11.03AVVKK99 pKa = 11.03VEE101 pKa = 4.21FTKK104 pKa = 10.58EE105 pKa = 3.65KK106 pKa = 9.44PVVYY110 pKa = 10.19RR111 pKa = 11.84EE112 pKa = 4.07ALVGIEE118 pKa = 4.18DD119 pKa = 4.36LEE121 pKa = 4.75GVDD124 pKa = 4.1EE125 pKa = 5.61DD126 pKa = 4.93DD127 pKa = 4.38FFGFGVDD134 pKa = 4.43AGLGCIADD142 pKa = 4.21AEE144 pKa = 4.36VLPYY148 pKa = 10.23VDD150 pKa = 4.62KK151 pKa = 11.44FEE153 pKa = 6.52AEE155 pKa = 3.84LDD157 pKa = 3.55VDD159 pKa = 5.0NMYY162 pKa = 11.36DD163 pKa = 3.76DD164 pKa = 4.48YY165 pKa = 11.53FAGLFAQSYY174 pKa = 9.8KK175 pKa = 10.81DD176 pKa = 3.36HH177 pKa = 7.47PNNQRR182 pKa = 11.84DD183 pKa = 3.45AGDD186 pKa = 4.08WINWTVPNTDD196 pKa = 3.05YY197 pKa = 10.66QIPMFASGFGDD208 pKa = 3.74GSYY211 pKa = 10.54PVYY214 pKa = 10.48FGYY217 pKa = 10.59DD218 pKa = 3.23ANDD221 pKa = 3.8EE222 pKa = 4.42VCGLYY227 pKa = 10.0IQFIDD232 pKa = 3.69IEE234 pKa = 4.38LALSEE239 pKa = 5.49DD240 pKa = 4.42GDD242 pKa = 4.34DD243 pKa = 5.36EE244 pKa = 6.34DD245 pKa = 6.49YY246 pKa = 11.62EE247 pKa = 6.59DD248 pKa = 5.99DD249 pKa = 5.22DD250 pKa = 4.15EE251 pKa = 5.76PEE253 pKa = 4.04NFVFLKK259 pKa = 10.81DD260 pKa = 3.33

Molecular weight: 29.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q4F3C9|A0A0Q4F3C9_9FLAO HARE-HTH domain-containing protein OS=Chryseobacterium sp. Leaf201 OX=1735672 GN=ASE55_19365 PE=4 SV=1
MM1 pKa = 7.54TGTLRR6 pKa = 11.84HH7 pKa = 5.83FFGSSSSLLRR17 pKa = 11.84VRR19 pKa = 11.84FGKK22 pKa = 8.98TGLFPKK28 pKa = 10.53NSRR31 pKa = 11.84TKK33 pKa = 10.73VGEE36 pKa = 4.17CPEE39 pKa = 3.95NRR41 pKa = 11.84AFPAGYY47 pKa = 9.47SCPEE51 pKa = 3.65ALTFTFNEE59 pKa = 3.93FLIFSLFIYY68 pKa = 9.88RR69 pKa = 11.84FHH71 pKa = 7.23KK72 pKa = 10.27HH73 pKa = 6.3RR74 pKa = 11.84IPYY77 pKa = 9.14FRR79 pKa = 11.84VRR81 pKa = 11.84SGSSCSFVNAYY92 pKa = 9.91FFVLLPP98 pKa = 4.12

Molecular weight: 11.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3820

0

3820

1236993

44

3409

323.8

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.429 ± 0.04

0.745 ± 0.011

5.472 ± 0.03

6.567 ± 0.046

5.336 ± 0.03

6.509 ± 0.037

1.765 ± 0.018

7.623 ± 0.036

7.871 ± 0.042

8.845 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.02

6.192 ± 0.046

3.543 ± 0.022

3.543 ± 0.024

3.567 ± 0.026

6.609 ± 0.032

5.678 ± 0.042

6.055 ± 0.032

1.047 ± 0.014

4.215 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski