Microbacterium sp. No. 7

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Microbacterium; unclassified Microbacterium

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4065 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P0DI93|A0A0P0DI93_9MICO ABC transporter domain-containing protein OS=Microbacterium sp. No. 7 OX=1714373 GN=AOA12_04750 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84HH3 pKa = 6.02RR4 pKa = 11.84LVGAAVLAALWMCTGCAASDD24 pKa = 4.03DD25 pKa = 4.66LGDD28 pKa = 4.27AADD31 pKa = 4.28GLSGGVPVGGLDD43 pKa = 3.63APADD47 pKa = 3.93PDD49 pKa = 3.59VDD51 pKa = 3.98PGADD55 pKa = 3.27ADD57 pKa = 4.13VPDD60 pKa = 5.0DD61 pKa = 4.51RR62 pKa = 11.84GDD64 pKa = 3.61VPPEE68 pKa = 3.77CLAALPYY75 pKa = 10.97LLGPADD81 pKa = 4.36FADD84 pKa = 3.74MDD86 pKa = 4.81APAGWPDD93 pKa = 3.51APADD97 pKa = 3.58ATLCMTASGDD107 pKa = 3.63VASATFVTEE116 pKa = 5.02LPFTDD121 pKa = 3.47VAAHH125 pKa = 5.51YY126 pKa = 9.12EE127 pKa = 3.99RR128 pKa = 11.84ALAEE132 pKa = 4.17TVHH135 pKa = 6.68ADD137 pKa = 2.95TYY139 pKa = 10.5EE140 pKa = 4.02VYY142 pKa = 9.08RR143 pKa = 11.84TSGEE147 pKa = 4.21EE148 pKa = 3.6NGTGYY153 pKa = 10.87DD154 pKa = 3.54ALDD157 pKa = 4.6GIGPEE162 pKa = 3.66GVMFQVRR169 pKa = 11.84EE170 pKa = 3.87HH171 pKa = 7.02DD172 pKa = 3.46AGFVLVLDD180 pKa = 4.27RR181 pKa = 11.84TT182 pKa = 4.05

Molecular weight: 18.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P0DN14|A0A0P0DN14_9MICO Uncharacterized protein OS=Microbacterium sp. No. 7 OX=1714373 GN=AOA12_06985 PE=4 SV=1
MM1 pKa = 7.22IPKK4 pKa = 9.88RR5 pKa = 11.84SARR8 pKa = 11.84ARR10 pKa = 11.84RR11 pKa = 11.84ATISEE16 pKa = 4.14RR17 pKa = 11.84PARR20 pKa = 11.84RR21 pKa = 11.84ARR23 pKa = 11.84ASSPRR28 pKa = 11.84ASASRR33 pKa = 11.84RR34 pKa = 11.84VSAAWSATSARR45 pKa = 11.84AAARR49 pKa = 11.84ARR51 pKa = 11.84AGAGLTSRR59 pKa = 11.84TASRR63 pKa = 11.84SAMSRR68 pKa = 11.84WLSTT72 pKa = 3.22

Molecular weight: 7.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4065

0

4065

1310660

38

1858

322.4

34.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.089 ± 0.066

0.547 ± 0.01

6.219 ± 0.032

5.591 ± 0.04

2.995 ± 0.022

9.079 ± 0.036

2.046 ± 0.017

4.421 ± 0.027

1.694 ± 0.027

10.112 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.782 ± 0.016

1.773 ± 0.02

5.519 ± 0.031

2.691 ± 0.02

7.771 ± 0.044

5.219 ± 0.024

5.937 ± 0.033

9.078 ± 0.039

1.509 ± 0.015

1.927 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski