Black medic leafroll alphasatellite 1

Taxonomy: Viruses; Alphasatellitidae; Nanoalphasatellitinae; unclassified Nanoalphasatellitinae

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9TQB9|V9TQB9_9VIRU Satellite replication initiator protein 1 OS=Black medic leafroll alphasatellite 1 OX=1441796 GN=para-Rep1 PE=4 SV=1
MM1 pKa = 7.53PTLQGTFWCFTLNFSGDD18 pKa = 3.46VPALSFDD25 pKa = 3.43EE26 pKa = 4.52TVQYY30 pKa = 11.08AVWQHH35 pKa = 5.28EE36 pKa = 4.94CVSHH40 pKa = 7.29DD41 pKa = 3.53HH42 pKa = 6.32LQGFVQMKK50 pKa = 9.75KK51 pKa = 9.78RR52 pKa = 11.84STMKK56 pKa = 9.66MMKK59 pKa = 9.62ILLPGAHH66 pKa = 6.74LEE68 pKa = 4.4VAKK71 pKa = 8.89GTPEE75 pKa = 3.93QASSYY80 pKa = 10.94AMKK83 pKa = 10.3EE84 pKa = 4.07SSRR87 pKa = 11.84VAGPWFYY94 pKa = 11.83GEE96 pKa = 4.34LLKK99 pKa = 11.07KK100 pKa = 10.73GSNKK104 pKa = 9.76RR105 pKa = 11.84KK106 pKa = 9.9LLDD109 pKa = 3.68RR110 pKa = 11.84YY111 pKa = 10.09RR112 pKa = 11.84DD113 pKa = 3.65SPEE116 pKa = 4.2DD117 pKa = 3.59MEE119 pKa = 6.32LEE121 pKa = 4.63DD122 pKa = 3.44PAKK125 pKa = 10.57ARR127 pKa = 11.84RR128 pKa = 11.84CRR130 pKa = 11.84ARR132 pKa = 11.84IDD134 pKa = 3.38KK135 pKa = 10.36EE136 pKa = 4.22KK137 pKa = 10.45FVSDD141 pKa = 3.88FKK143 pKa = 11.74VEE145 pKa = 4.44DD146 pKa = 5.3DD147 pKa = 3.63EE148 pKa = 5.53QEE150 pKa = 3.74WKK152 pKa = 10.73RR153 pKa = 11.84LVEE156 pKa = 3.97EE157 pKa = 4.27EE158 pKa = 4.48LNIEE162 pKa = 4.16ASPRR166 pKa = 11.84SIIWVYY172 pKa = 10.53GPQGGEE178 pKa = 3.96GKK180 pKa = 6.19TTKK183 pKa = 10.45AKK185 pKa = 10.55EE186 pKa = 4.01LITRR190 pKa = 11.84GWFYY194 pKa = 11.12TRR196 pKa = 11.84GGKK199 pKa = 9.58KK200 pKa = 10.11DD201 pKa = 3.72DD202 pKa = 3.44VAYY205 pKa = 10.69SYY207 pKa = 11.84VEE209 pKa = 4.04DD210 pKa = 3.89PTRR213 pKa = 11.84HH214 pKa = 4.54VVFDD218 pKa = 4.31IPRR221 pKa = 11.84DD222 pKa = 3.66MQDD225 pKa = 2.88YY226 pKa = 10.68CNYY229 pKa = 10.91SLIEE233 pKa = 3.98MLKK236 pKa = 10.7DD237 pKa = 4.34RR238 pKa = 11.84IIISNKK244 pKa = 9.28YY245 pKa = 9.83EE246 pKa = 5.03PITNCQVANIHH257 pKa = 6.08VIVMANFLPDD267 pKa = 3.2VTKK270 pKa = 10.74ISNDD274 pKa = 3.1RR275 pKa = 11.84LKK277 pKa = 10.52IIYY280 pKa = 9.59CC281 pKa = 4.02

Molecular weight: 32.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9TQB9|V9TQB9_9VIRU Satellite replication initiator protein 1 OS=Black medic leafroll alphasatellite 1 OX=1441796 GN=para-Rep1 PE=4 SV=1
MM1 pKa = 7.53PTLQGTFWCFTLNFSGDD18 pKa = 3.46VPALSFDD25 pKa = 3.43EE26 pKa = 4.52TVQYY30 pKa = 11.08AVWQHH35 pKa = 5.28EE36 pKa = 4.94CVSHH40 pKa = 7.29DD41 pKa = 3.53HH42 pKa = 6.32LQGFVQMKK50 pKa = 9.75KK51 pKa = 9.78RR52 pKa = 11.84STMKK56 pKa = 9.66MMKK59 pKa = 9.62ILLPGAHH66 pKa = 6.74LEE68 pKa = 4.4VAKK71 pKa = 8.89GTPEE75 pKa = 3.93QASSYY80 pKa = 10.94AMKK83 pKa = 10.3EE84 pKa = 4.07SSRR87 pKa = 11.84VAGPWFYY94 pKa = 11.83GEE96 pKa = 4.34LLKK99 pKa = 11.07KK100 pKa = 10.73GSNKK104 pKa = 9.76RR105 pKa = 11.84KK106 pKa = 9.9LLDD109 pKa = 3.68RR110 pKa = 11.84YY111 pKa = 10.09RR112 pKa = 11.84DD113 pKa = 3.65SPEE116 pKa = 4.2DD117 pKa = 3.59MEE119 pKa = 6.32LEE121 pKa = 4.63DD122 pKa = 3.44PAKK125 pKa = 10.57ARR127 pKa = 11.84RR128 pKa = 11.84CRR130 pKa = 11.84ARR132 pKa = 11.84IDD134 pKa = 3.38KK135 pKa = 10.36EE136 pKa = 4.22KK137 pKa = 10.45FVSDD141 pKa = 3.88FKK143 pKa = 11.74VEE145 pKa = 4.44DD146 pKa = 5.3DD147 pKa = 3.63EE148 pKa = 5.53QEE150 pKa = 3.74WKK152 pKa = 10.73RR153 pKa = 11.84LVEE156 pKa = 3.97EE157 pKa = 4.27EE158 pKa = 4.48LNIEE162 pKa = 4.16ASPRR166 pKa = 11.84SIIWVYY172 pKa = 10.53GPQGGEE178 pKa = 3.96GKK180 pKa = 6.19TTKK183 pKa = 10.45AKK185 pKa = 10.55EE186 pKa = 4.01LITRR190 pKa = 11.84GWFYY194 pKa = 11.12TRR196 pKa = 11.84GGKK199 pKa = 9.58KK200 pKa = 10.11DD201 pKa = 3.72DD202 pKa = 3.44VAYY205 pKa = 10.69SYY207 pKa = 11.84VEE209 pKa = 4.04DD210 pKa = 3.89PTRR213 pKa = 11.84HH214 pKa = 4.54VVFDD218 pKa = 4.31IPRR221 pKa = 11.84DD222 pKa = 3.66MQDD225 pKa = 2.88YY226 pKa = 10.68CNYY229 pKa = 10.91SLIEE233 pKa = 3.98MLKK236 pKa = 10.7DD237 pKa = 4.34RR238 pKa = 11.84IIISNKK244 pKa = 9.28YY245 pKa = 9.83EE246 pKa = 5.03PITNCQVANIHH257 pKa = 6.08VIVMANFLPDD267 pKa = 3.2VTKK270 pKa = 10.74ISNDD274 pKa = 3.1RR275 pKa = 11.84LKK277 pKa = 10.52IIYY280 pKa = 9.59CC281 pKa = 4.02

Molecular weight: 32.58 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

281

281

281

281.0

32.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.338 ± 0.0

2.135 ± 0.0

7.117 ± 0.0

7.829 ± 0.0

3.915 ± 0.0

5.338 ± 0.0

2.135 ± 0.0

6.05 ± 0.0

8.541 ± 0.0

6.762 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.559 ± 0.0

3.203 ± 0.0

4.626 ± 0.0

3.559 ± 0.0

6.05 ± 0.0

6.05 ± 0.0

4.626 ± 0.0

6.762 ± 0.0

2.135 ± 0.0

4.27 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski