Citrus virus A

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Phenuiviridae; Coguvirus; Coguvirus eburi

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G2LX79|A0A3G2LX79_9VIRU Nucleocapsid protein OS=Citrus virus A OX=2484986 GN=s2gp2 PE=4 SV=1
MM1 pKa = 7.83ALHH4 pKa = 6.12QNPKK8 pKa = 9.28EE9 pKa = 3.97QSKK12 pKa = 10.74FDD14 pKa = 3.93ALFSQATSHH23 pKa = 6.52GEE25 pKa = 3.39EE26 pKa = 4.6HH27 pKa = 6.31YY28 pKa = 11.06RR29 pKa = 11.84EE30 pKa = 4.15FLTKK34 pKa = 10.39CSKK37 pKa = 10.22NLKK40 pKa = 9.41EE41 pKa = 4.19RR42 pKa = 11.84IKK44 pKa = 10.98RR45 pKa = 11.84EE46 pKa = 3.63VNARR50 pKa = 11.84KK51 pKa = 7.13MTSGGDD57 pKa = 3.46FLEE60 pKa = 4.77SFEE63 pKa = 4.2QAEE66 pKa = 4.44DD67 pKa = 3.69KK68 pKa = 11.03SGKK71 pKa = 9.77LDD73 pKa = 3.58NKK75 pKa = 10.32EE76 pKa = 4.09GEE78 pKa = 4.3LEE80 pKa = 4.03EE81 pKa = 5.84DD82 pKa = 3.2EE83 pKa = 6.07HH84 pKa = 8.65EE85 pKa = 4.96DD86 pKa = 3.46EE87 pKa = 4.33EE88 pKa = 5.19QEE90 pKa = 4.18VTEE93 pKa = 4.48GSGTKK98 pKa = 9.86KK99 pKa = 10.78DD100 pKa = 3.51IVVTDD105 pKa = 3.87AQLAAMWTEE114 pKa = 3.45IDD116 pKa = 4.28NIDD119 pKa = 3.33VSTFDD124 pKa = 3.68RR125 pKa = 11.84EE126 pKa = 4.29STKK129 pKa = 10.48VFDD132 pKa = 4.13YY133 pKa = 11.26QGFNPDD139 pKa = 2.93EE140 pKa = 4.27VLRR143 pKa = 11.84SLIINQRR150 pKa = 11.84KK151 pKa = 9.01NKK153 pKa = 9.7VSAADD158 pKa = 3.9FKK160 pKa = 11.69SDD162 pKa = 3.24ILLMCSLAIIKK173 pKa = 10.47GSINEE178 pKa = 4.18HH179 pKa = 5.25NFKK182 pKa = 10.71KK183 pKa = 10.67LSTEE187 pKa = 4.02GQSTVIRR194 pKa = 11.84LEE196 pKa = 3.52KK197 pKa = 10.08TYY199 pKa = 10.67GIKK202 pKa = 10.27RR203 pKa = 11.84GSGRR207 pKa = 11.84SEE209 pKa = 3.75PANVVTISRR218 pKa = 11.84IGATFPGKK226 pKa = 10.09IIQLLVEE233 pKa = 4.65NKK235 pKa = 9.44ASSRR239 pKa = 11.84KK240 pKa = 9.64FMGPLKK246 pKa = 10.67SHH248 pKa = 6.97TLPQFIRR255 pKa = 11.84HH256 pKa = 5.34QGFAAVIPTTLAEE269 pKa = 4.21STRR272 pKa = 11.84MFLLDD277 pKa = 3.28IVTAYY282 pKa = 10.73SVDD285 pKa = 3.47QSISISPNKK294 pKa = 9.38KK295 pKa = 9.8NKK297 pKa = 9.23PDD299 pKa = 3.57LASLYY304 pKa = 9.42EE305 pKa = 4.07KK306 pKa = 10.35QRR308 pKa = 11.84SFIQTTADD316 pKa = 2.86NKK318 pKa = 10.03YY319 pKa = 8.95PSEE322 pKa = 4.01IVRR325 pKa = 11.84KK326 pKa = 10.14KK327 pKa = 10.35IFTTIMIDD335 pKa = 3.46YY336 pKa = 10.42DD337 pKa = 3.97SLLTTARR344 pKa = 11.84ALLKK348 pKa = 9.94VIPDD352 pKa = 3.36FTIVTKK358 pKa = 10.76EE359 pKa = 3.92NFVSDD364 pKa = 3.6INSTHH369 pKa = 5.88II370 pKa = 3.9

Molecular weight: 41.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G2LX79|A0A3G2LX79_9VIRU Nucleocapsid protein OS=Citrus virus A OX=2484986 GN=s2gp2 PE=4 SV=1
MM1 pKa = 7.14FKK3 pKa = 10.56KK4 pKa = 10.39VFRR7 pKa = 11.84RR8 pKa = 11.84ASGTPKK14 pKa = 10.21PVLQQATLQNRR25 pKa = 11.84PQRR28 pKa = 11.84QDD30 pKa = 2.47TDD32 pKa = 4.63SNVSRR37 pKa = 11.84AGKK40 pKa = 9.99NEE42 pKa = 3.65LVADD46 pKa = 4.77EE47 pKa = 4.5SLKK50 pKa = 9.97TADD53 pKa = 3.6RR54 pKa = 11.84NLYY57 pKa = 10.59KK58 pKa = 10.3FDD60 pKa = 4.75LPNNEE65 pKa = 4.21QGFDD69 pKa = 3.94DD70 pKa = 4.11LAKK73 pKa = 10.06EE74 pKa = 4.13YY75 pKa = 11.3SEE77 pKa = 4.17GTRR80 pKa = 11.84FQYY83 pKa = 10.77VALSGKK89 pKa = 10.1DD90 pKa = 3.41EE91 pKa = 4.16EE92 pKa = 5.13SYY94 pKa = 10.45SARR97 pKa = 11.84IRR99 pKa = 11.84RR100 pKa = 11.84LVTLTPRR107 pKa = 11.84MNKK110 pKa = 8.37DD111 pKa = 3.32TLPLTKK117 pKa = 9.59IPQLKK122 pKa = 10.17LIEE125 pKa = 4.54DD126 pKa = 4.25TEE128 pKa = 4.27QFNLSLAVPKK138 pKa = 10.34KK139 pKa = 8.27GKK141 pKa = 9.68PYY143 pKa = 10.03IRR145 pKa = 11.84LASVTAYY152 pKa = 9.89YY153 pKa = 10.3CPLVSSFSEE162 pKa = 4.32FTKK165 pKa = 10.91AGMSLHH171 pKa = 7.17DD172 pKa = 4.65SRR174 pKa = 11.84LSSDD178 pKa = 3.39TSVQSVTFNSNITQKK193 pKa = 11.23LEE195 pKa = 3.83LSLDD199 pKa = 3.47YY200 pKa = 10.88CIPRR204 pKa = 11.84SSASKK209 pKa = 8.53ITLNIARR216 pKa = 11.84EE217 pKa = 4.02QKK219 pKa = 9.71FLKK222 pKa = 10.56EE223 pKa = 3.98GEE225 pKa = 4.02EE226 pKa = 4.0WGAVQLLIKK235 pKa = 10.57LEE237 pKa = 4.24EE238 pKa = 4.31SDD240 pKa = 6.05FPYY243 pKa = 10.7CSNLKK248 pKa = 9.96EE249 pKa = 4.16ATAVMALPPAVLDD262 pKa = 3.72SHH264 pKa = 6.57NVNPNHH270 pKa = 7.06IDD272 pKa = 3.29TTIVEE277 pKa = 4.3TQRR280 pKa = 11.84RR281 pKa = 11.84KK282 pKa = 9.91VRR284 pKa = 11.84EE285 pKa = 3.67IYY287 pKa = 10.73EE288 pKa = 4.2NGDD291 pKa = 2.74ISNEE295 pKa = 3.86NEE297 pKa = 4.46PIKK300 pKa = 10.98EE301 pKa = 3.95MLGKK305 pKa = 10.35VSYY308 pKa = 10.33AKK310 pKa = 10.48SSISAKK316 pKa = 10.59VKK318 pKa = 9.66GQKK321 pKa = 7.78TNKK324 pKa = 9.71KK325 pKa = 9.22FGKK328 pKa = 9.36GWEE331 pKa = 4.01HH332 pKa = 6.12MEE334 pKa = 3.8FRR336 pKa = 11.84FPKK339 pKa = 8.45ITKK342 pKa = 9.1DD343 pKa = 3.45QEE345 pKa = 4.3SEE347 pKa = 4.08EE348 pKa = 4.31PEE350 pKa = 3.97EE351 pKa = 4.98DD352 pKa = 3.57GSIATPSLGSGRR364 pKa = 11.84NSVQEE369 pKa = 3.79IEE371 pKa = 4.48EE372 pKa = 4.23VVGTSEE378 pKa = 4.08TKK380 pKa = 10.23KK381 pKa = 10.0VNFNKK386 pKa = 10.08GVKK389 pKa = 9.8FNEE392 pKa = 4.37FGVV395 pKa = 3.64

Molecular weight: 44.47 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2949

370

2184

983.0

112.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.68 ± 0.562

1.492 ± 0.439

5.866 ± 0.352

7.765 ± 0.555

4.747 ± 0.165

4.646 ± 0.142

1.831 ± 0.273

7.426 ± 0.961

8.647 ± 0.339

9.257 ± 0.678

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.34 ± 0.352

4.51 ± 0.466

3.594 ± 0.41

3.357 ± 0.442

4.781 ± 0.098

9.19 ± 0.15

5.866 ± 0.586

5.561 ± 0.261

1.051 ± 0.346

3.391 ± 0.557
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski