Pseudomonas phage Epa5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.65

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G9LI61|A0A6G9LI61_9CAUD DNA-directed DNA polymerase OS=Pseudomonas phage Epa5 OX=2719575 GN=Epa5_55 PE=3 SV=1
MM1 pKa = 7.66ASVTLAEE8 pKa = 4.3SAKK11 pKa = 10.49LALDD15 pKa = 3.59EE16 pKa = 4.73LVAGVIEE23 pKa = 4.52NVITVNRR30 pKa = 11.84MFEE33 pKa = 4.34VIPFDD38 pKa = 4.66GISGNALAYY47 pKa = 10.38NRR49 pKa = 11.84EE50 pKa = 4.01NVLGNVQVAGVDD62 pKa = 3.4ATITAKK68 pKa = 10.84APATFTKK75 pKa = 9.51VTSEE79 pKa = 3.93LTTIIGDD86 pKa = 3.74AEE88 pKa = 4.54VNGLIQATRR97 pKa = 11.84SSDD100 pKa = 3.88GNDD103 pKa = 2.98QTAVQVASKK112 pKa = 10.7AKK114 pKa = 9.2SCGRR118 pKa = 11.84KK119 pKa = 8.46YY120 pKa = 11.07QDD122 pKa = 3.21MLINGTGASDD132 pKa = 3.54EE133 pKa = 4.34FEE135 pKa = 5.29GIINLCAEE143 pKa = 4.37GQKK146 pKa = 10.36VATGDD151 pKa = 3.42NGSALSFEE159 pKa = 4.68ILDD162 pKa = 3.73EE163 pKa = 4.74LMDD166 pKa = 4.44LVVDD170 pKa = 4.24KK171 pKa = 10.99DD172 pKa = 3.87GQVDD176 pKa = 4.53YY177 pKa = 9.88LTMHH181 pKa = 6.85ARR183 pKa = 11.84TLRR186 pKa = 11.84SYY188 pKa = 11.04NALLRR193 pKa = 11.84SLGGASIGDD202 pKa = 4.02VVTLPSGAEE211 pKa = 3.83VPAYY215 pKa = 9.93RR216 pKa = 11.84GVPIFRR222 pKa = 11.84NDD224 pKa = 3.92YY225 pKa = 10.8IPTNQTKK232 pKa = 8.19GTGTNTTSIFAGTLDD247 pKa = 4.29DD248 pKa = 4.21GSRR251 pKa = 11.84THH253 pKa = 6.74GLAGLTAEE261 pKa = 4.28EE262 pKa = 3.92AAGIRR267 pKa = 11.84VEE269 pKa = 4.42DD270 pKa = 3.71VGPAEE275 pKa = 5.69DD276 pKa = 3.73KK277 pKa = 11.05DD278 pKa = 4.03NYY280 pKa = 7.42ITRR283 pKa = 11.84VKK285 pKa = 9.32WYY287 pKa = 9.91CGLALFSEE295 pKa = 5.12KK296 pKa = 10.23GLAMADD302 pKa = 4.22GITNN306 pKa = 3.92

Molecular weight: 32.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G9LI61|A0A6G9LI61_9CAUD DNA-directed DNA polymerase OS=Pseudomonas phage Epa5 OX=2719575 GN=Epa5_55 PE=3 SV=1
MM1 pKa = 6.75MQVPLLTPFSGWKK14 pKa = 10.58APDD17 pKa = 3.72LNALPSWAGHH27 pKa = 5.27KK28 pKa = 10.16RR29 pKa = 11.84IAVDD33 pKa = 4.09CEE35 pKa = 4.06TRR37 pKa = 11.84DD38 pKa = 3.74PFLKK42 pKa = 10.13TLGPGVRR49 pKa = 11.84RR50 pKa = 11.84GGDD53 pKa = 3.18SYY55 pKa = 11.91LVGISFAIEE64 pKa = 4.57DD65 pKa = 4.42GPSGYY70 pKa = 10.68LPIAHH75 pKa = 7.26AGGGNLPKK83 pKa = 10.8EE84 pKa = 4.2MVLKK88 pKa = 10.69YY89 pKa = 10.06MKK91 pKa = 10.52DD92 pKa = 3.06QAAVFDD98 pKa = 4.38GEE100 pKa = 4.36IVGANLQYY108 pKa = 11.38DD109 pKa = 5.02LDD111 pKa = 3.71WLAQYY116 pKa = 10.77GIVFRR121 pKa = 11.84RR122 pKa = 11.84AKK124 pKa = 10.2YY125 pKa = 10.03FRR127 pKa = 11.84DD128 pKa = 3.66VQVAEE133 pKa = 4.12PLIDD137 pKa = 4.0EE138 pKa = 4.78LQLSYY143 pKa = 11.57SLDD146 pKa = 4.1NIAKK150 pKa = 9.08RR151 pKa = 11.84HH152 pKa = 5.54GIAGKK157 pKa = 10.35DD158 pKa = 3.35EE159 pKa = 4.23TLLMQAARR167 pKa = 11.84AYY169 pKa = 10.13GIHH172 pKa = 6.85PKK174 pKa = 10.01KK175 pKa = 10.7DD176 pKa = 3.17LWRR179 pKa = 11.84LPAQFVGPYY188 pKa = 9.79AEE190 pKa = 4.21EE191 pKa = 4.33DD192 pKa = 3.3ARR194 pKa = 11.84LPLQLLRR201 pKa = 11.84RR202 pKa = 11.84QEE204 pKa = 4.38RR205 pKa = 11.84IIDD208 pKa = 3.87DD209 pKa = 3.64QEE211 pKa = 3.4IWDD214 pKa = 4.13IYY216 pKa = 10.11NLEE219 pKa = 4.4SKK221 pKa = 10.35VLPVLVKK228 pKa = 8.76MRR230 pKa = 11.84RR231 pKa = 11.84RR232 pKa = 11.84GVRR235 pKa = 11.84VHH237 pKa = 6.83EE238 pKa = 4.58GRR240 pKa = 11.84LTEE243 pKa = 3.82IEE245 pKa = 4.13RR246 pKa = 11.84WSLQQEE252 pKa = 4.49TEE254 pKa = 3.6ALAQVRR260 pKa = 11.84HH261 pKa = 4.8LTGVNIAVGDD271 pKa = 3.67VWKK274 pKa = 11.21NEE276 pKa = 3.73ALAPALTHH284 pKa = 7.28IGLKK288 pKa = 10.18LNKK291 pKa = 8.46TAEE294 pKa = 4.45GKK296 pKa = 10.67VSIDD300 pKa = 3.26KK301 pKa = 10.75EE302 pKa = 4.02FLAGVDD308 pKa = 3.36HH309 pKa = 7.44DD310 pKa = 3.94VARR313 pKa = 11.84ALEE316 pKa = 4.16RR317 pKa = 11.84ARR319 pKa = 11.84KK320 pKa = 6.8TNKK323 pKa = 9.74LRR325 pKa = 11.84TTFAASVRR333 pKa = 11.84EE334 pKa = 3.85HH335 pKa = 5.78MVNGRR340 pKa = 11.84IHH342 pKa = 6.47ATFNQLRR349 pKa = 11.84MEE351 pKa = 4.89KK352 pKa = 10.76DD353 pKa = 3.09NGDD356 pKa = 3.82LGGAAYY362 pKa = 10.06GRR364 pKa = 11.84LSCVQPNLQQQPARR378 pKa = 11.84DD379 pKa = 4.23DD380 pKa = 3.69FAKK383 pKa = 8.7MWRR386 pKa = 11.84GIYY389 pKa = 10.17LPEE392 pKa = 4.96EE393 pKa = 4.4GQLWAALDD401 pKa = 3.92YY402 pKa = 10.73SQQEE406 pKa = 3.97PRR408 pKa = 11.84MTVHH412 pKa = 6.99FAEE415 pKa = 4.76LCGLPGSKK423 pKa = 9.93EE424 pKa = 3.91AGDD427 pKa = 4.57KK428 pKa = 10.99YY429 pKa = 10.26RR430 pKa = 11.84TDD432 pKa = 3.86PNADD436 pKa = 3.33NHH438 pKa = 6.83QMMADD443 pKa = 3.3MAKK446 pKa = 9.59IQRR449 pKa = 11.84KK450 pKa = 6.72AAKK453 pKa = 9.77EE454 pKa = 3.77IFLGLCYY461 pKa = 10.67GMGGAKK467 pKa = 10.15LCRR470 pKa = 11.84KK471 pKa = 9.83LGLPTAWAVQHH482 pKa = 6.29RR483 pKa = 11.84NIRR486 pKa = 11.84GLVALDD492 pKa = 3.46TEE494 pKa = 5.03RR495 pKa = 11.84GQEE498 pKa = 4.18LLRR501 pKa = 11.84QGGKK505 pKa = 9.73KK506 pKa = 10.33LEE508 pKa = 4.09IAGPEE513 pKa = 4.22GQALLDD519 pKa = 3.71TFDD522 pKa = 3.87AKK524 pKa = 11.29VPFVRR529 pKa = 11.84QLARR533 pKa = 11.84RR534 pKa = 11.84CSKK537 pKa = 9.93VAAEE541 pKa = 4.38RR542 pKa = 11.84GYY544 pKa = 11.58LMTLAGRR551 pKa = 11.84RR552 pKa = 11.84CRR554 pKa = 11.84FPKK557 pKa = 10.09TPEE560 pKa = 3.69GKK562 pKa = 10.36YY563 pKa = 10.37DD564 pKa = 3.5WTHH567 pKa = 6.21KK568 pKa = 10.48GLNRR572 pKa = 11.84LIQGSSGDD580 pKa = 3.62QTKK583 pKa = 8.35TAMVMLDD590 pKa = 3.46EE591 pKa = 5.28AGHH594 pKa = 5.34YY595 pKa = 10.02LQLQVHH601 pKa = 6.99DD602 pKa = 4.57EE603 pKa = 4.24MDD605 pKa = 3.74GSVQSPEE612 pKa = 3.82EE613 pKa = 4.36AEE615 pKa = 4.04AMAEE619 pKa = 3.99IMRR622 pKa = 11.84TCTPLTVPSKK632 pKa = 11.28VDD634 pKa = 3.44VEE636 pKa = 5.45LGASWGEE643 pKa = 4.25SMSS646 pKa = 3.5

Molecular weight: 72.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2495

180

790

415.8

46.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.499 ± 0.762

0.882 ± 0.179

6.573 ± 0.411

7.174 ± 0.497

3.768 ± 0.485

8.617 ± 0.498

1.924 ± 0.473

4.489 ± 0.47

6.052 ± 0.749

8.858 ± 0.698

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.645 ± 0.342

3.567 ± 0.434

4.81 ± 0.457

4.329 ± 0.469

5.972 ± 0.546

4.97 ± 0.651

5.13 ± 1.203

6.653 ± 0.372

1.242 ± 0.38

2.846 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski