Amino acid dipepetide frequency for Rhodococcus phage Espica

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.999AlaAla: 9.999 ± 1.334
0.559AlaCys: 0.559 ± 0.241
5.384AlaAsp: 5.384 ± 0.639
9.02AlaGlu: 9.02 ± 0.843
2.867AlaPhe: 2.867 ± 0.422
7.621AlaGly: 7.621 ± 0.84
1.189AlaHis: 1.189 ± 0.329
4.195AlaIle: 4.195 ± 0.639
3.916AlaLys: 3.916 ± 0.471
8.95AlaLeu: 8.95 ± 0.942
2.307AlaMet: 2.307 ± 0.298
3.007AlaAsn: 3.007 ± 0.508
4.125AlaPro: 4.125 ± 0.68
3.286AlaGln: 3.286 ± 0.589
5.943AlaArg: 5.943 ± 0.579
5.314AlaSer: 5.314 ± 0.525
4.685AlaThr: 4.685 ± 0.695
7.272AlaVal: 7.272 ± 0.6
1.608AlaTrp: 1.608 ± 0.329
2.587AlaTyr: 2.587 ± 0.469
0.0AlaXaa: 0.0 ± 0.0
Cys
0.35CysAla: 0.35 ± 0.134
0.0CysCys: 0.0 ± 0.0
0.489CysAsp: 0.489 ± 0.179
0.769CysGlu: 0.769 ± 0.278
0.14CysPhe: 0.14 ± 0.092
1.189CysGly: 1.189 ± 0.26
0.14CysHis: 0.14 ± 0.083
0.21CysIle: 0.21 ± 0.111
0.559CysLys: 0.559 ± 0.222
0.28CysLeu: 0.28 ± 0.138
0.07CysMet: 0.07 ± 0.075
0.28CysAsn: 0.28 ± 0.108
0.699CysPro: 0.699 ± 0.211
0.42CysGln: 0.42 ± 0.157
0.489CysArg: 0.489 ± 0.203
0.489CysSer: 0.489 ± 0.198
0.35CysThr: 0.35 ± 0.166
0.699CysVal: 0.699 ± 0.262
0.14CysTrp: 0.14 ± 0.096
0.489CysTyr: 0.489 ± 0.191
0.0CysXaa: 0.0 ± 0.0
Asp
5.803AspAla: 5.803 ± 0.767
0.699AspCys: 0.699 ± 0.229
3.076AspAsp: 3.076 ± 0.394
5.174AspGlu: 5.174 ± 0.726
1.958AspPhe: 1.958 ± 0.385
5.873AspGly: 5.873 ± 0.632
1.888AspHis: 1.888 ± 0.385
1.328AspIle: 1.328 ± 0.314
3.146AspLys: 3.146 ± 0.52
5.873AspLeu: 5.873 ± 0.59
2.098AspMet: 2.098 ± 0.349
2.307AspAsn: 2.307 ± 0.426
4.755AspPro: 4.755 ± 0.634
2.377AspGln: 2.377 ± 0.347
3.496AspArg: 3.496 ± 0.452
3.846AspSer: 3.846 ± 0.589
3.426AspThr: 3.426 ± 0.427
5.384AspVal: 5.384 ± 0.527
1.328AspTrp: 1.328 ± 0.341
2.517AspTyr: 2.517 ± 0.495
0.0AspXaa: 0.0 ± 0.0
Glu
7.272GluAla: 7.272 ± 0.938
0.559GluCys: 0.559 ± 0.192
5.314GluAsp: 5.314 ± 0.61
4.964GluGlu: 4.964 ± 0.849
2.657GluPhe: 2.657 ± 0.46
6.013GluGly: 6.013 ± 0.58
1.328GluHis: 1.328 ± 0.273
4.475GluIle: 4.475 ± 0.803
2.307GluLys: 2.307 ± 0.418
7.412GluLeu: 7.412 ± 0.951
1.119GluMet: 1.119 ± 0.275
2.727GluAsn: 2.727 ± 0.38
2.727GluPro: 2.727 ± 0.512
2.587GluGln: 2.587 ± 0.434
3.916GluArg: 3.916 ± 0.612
3.496GluSer: 3.496 ± 0.599
4.755GluThr: 4.755 ± 0.656
6.433GluVal: 6.433 ± 0.705
1.398GluTrp: 1.398 ± 0.403
1.818GluTyr: 1.818 ± 0.325
0.0GluXaa: 0.0 ± 0.0
Phe
3.426PheAla: 3.426 ± 0.543
0.21PheCys: 0.21 ± 0.127
2.657PheAsp: 2.657 ± 0.403
2.098PheGlu: 2.098 ± 0.341
0.699PhePhe: 0.699 ± 0.175
2.727PheGly: 2.727 ± 0.373
0.629PheHis: 0.629 ± 0.19
1.468PheIle: 1.468 ± 0.297
1.398PheLys: 1.398 ± 0.271
3.076PheLeu: 3.076 ± 0.578
0.699PheMet: 0.699 ± 0.192
1.189PheAsn: 1.189 ± 0.232
1.328PhePro: 1.328 ± 0.277
1.189PheGln: 1.189 ± 0.279
2.237PheArg: 2.237 ± 0.419
2.587PheSer: 2.587 ± 0.383
1.818PheThr: 1.818 ± 0.308
2.517PheVal: 2.517 ± 0.509
0.489PheTrp: 0.489 ± 0.143
1.049PheTyr: 1.049 ± 0.279
0.0PheXaa: 0.0 ± 0.0
Gly
6.852GlyAla: 6.852 ± 0.879
0.839GlyCys: 0.839 ± 0.231
6.433GlyAsp: 6.433 ± 0.58
4.894GlyGlu: 4.894 ± 0.694
4.545GlyPhe: 4.545 ± 0.552
6.293GlyGly: 6.293 ± 0.804
1.748GlyHis: 1.748 ± 0.354
4.055GlyIle: 4.055 ± 0.566
3.776GlyLys: 3.776 ± 0.49
5.733GlyLeu: 5.733 ± 0.73
2.237GlyMet: 2.237 ± 0.63
2.937GlyAsn: 2.937 ± 0.419
3.356GlyPro: 3.356 ± 0.409
3.706GlyGln: 3.706 ± 0.615
4.055GlyArg: 4.055 ± 0.588
5.244GlySer: 5.244 ± 0.767
5.454GlyThr: 5.454 ± 0.648
7.272GlyVal: 7.272 ± 0.773
2.168GlyTrp: 2.168 ± 0.38
3.216GlyTyr: 3.216 ± 0.615
0.0GlyXaa: 0.0 ± 0.0
His
1.398HisAla: 1.398 ± 0.281
0.07HisCys: 0.07 ± 0.063
1.538HisAsp: 1.538 ± 0.334
1.468HisGlu: 1.468 ± 0.369
0.699HisPhe: 0.699 ± 0.211
1.119HisGly: 1.119 ± 0.29
0.489HisHis: 0.489 ± 0.186
1.398HisIle: 1.398 ± 0.332
0.559HisLys: 0.559 ± 0.181
1.398HisLeu: 1.398 ± 0.368
0.629HisMet: 0.629 ± 0.247
0.559HisAsn: 0.559 ± 0.182
1.119HisPro: 1.119 ± 0.248
0.629HisGln: 0.629 ± 0.181
1.748HisArg: 1.748 ± 0.357
0.839HisSer: 0.839 ± 0.29
0.979HisThr: 0.979 ± 0.304
1.328HisVal: 1.328 ± 0.252
0.42HisTrp: 0.42 ± 0.184
0.839HisTyr: 0.839 ± 0.237
0.0HisXaa: 0.0 ± 0.0
Ile
5.314IleAla: 5.314 ± 0.653
0.42IleCys: 0.42 ± 0.195
3.706IleAsp: 3.706 ± 0.494
3.496IleGlu: 3.496 ± 0.692
0.909IlePhe: 0.909 ± 0.256
5.034IleGly: 5.034 ± 0.667
0.979IleHis: 0.979 ± 0.208
1.538IleIle: 1.538 ± 0.319
1.328IleLys: 1.328 ± 0.278
3.076IleLeu: 3.076 ± 0.466
0.769IleMet: 0.769 ± 0.189
1.328IleAsn: 1.328 ± 0.281
2.447IlePro: 2.447 ± 0.436
1.049IleGln: 1.049 ± 0.262
3.426IleArg: 3.426 ± 0.462
2.237IleSer: 2.237 ± 0.443
3.636IleThr: 3.636 ± 0.529
3.146IleVal: 3.146 ± 0.523
0.489IleTrp: 0.489 ± 0.191
1.678IleTyr: 1.678 ± 0.336
0.0IleXaa: 0.0 ± 0.0
Lys
3.846LysAla: 3.846 ± 0.59
0.14LysCys: 0.14 ± 0.086
2.447LysAsp: 2.447 ± 0.405
2.028LysGlu: 2.028 ± 0.356
1.119LysPhe: 1.119 ± 0.279
3.566LysGly: 3.566 ± 0.744
1.119LysHis: 1.119 ± 0.275
1.538LysIle: 1.538 ± 0.304
1.608LysLys: 1.608 ± 0.408
4.265LysLeu: 4.265 ± 0.439
0.979LysMet: 0.979 ± 0.279
1.398LysAsn: 1.398 ± 0.297
1.608LysPro: 1.608 ± 0.385
0.839LysGln: 0.839 ± 0.235
2.587LysArg: 2.587 ± 0.48
2.307LysSer: 2.307 ± 0.292
2.587LysThr: 2.587 ± 0.557
3.216LysVal: 3.216 ± 0.594
0.699LysTrp: 0.699 ± 0.209
0.979LysTyr: 0.979 ± 0.229
0.0LysXaa: 0.0 ± 0.0
Leu
9.789LeuAla: 9.789 ± 0.801
0.489LeuCys: 0.489 ± 0.175
5.524LeuAsp: 5.524 ± 0.79
6.153LeuGlu: 6.153 ± 0.779
2.377LeuPhe: 2.377 ± 0.299
6.503LeuGly: 6.503 ± 0.745
1.608LeuHis: 1.608 ± 0.361
3.356LeuIle: 3.356 ± 0.391
3.496LeuLys: 3.496 ± 0.505
5.314LeuLeu: 5.314 ± 0.665
1.818LeuMet: 1.818 ± 0.444
2.307LeuAsn: 2.307 ± 0.53
4.685LeuPro: 4.685 ± 0.471
2.867LeuGln: 2.867 ± 0.407
5.314LeuArg: 5.314 ± 0.699
5.384LeuSer: 5.384 ± 0.597
6.223LeuThr: 6.223 ± 0.747
6.013LeuVal: 6.013 ± 0.551
1.538LeuTrp: 1.538 ± 0.36
2.587LeuTyr: 2.587 ± 0.366
0.0LeuXaa: 0.0 ± 0.0
Met
2.028MetAla: 2.028 ± 0.412
0.0MetCys: 0.0 ± 0.0
1.189MetAsp: 1.189 ± 0.28
1.398MetGlu: 1.398 ± 0.311
0.839MetPhe: 0.839 ± 0.228
2.307MetGly: 2.307 ± 0.38
0.35MetHis: 0.35 ± 0.15
1.398MetIle: 1.398 ± 0.311
1.049MetLys: 1.049 ± 0.242
1.538MetLeu: 1.538 ± 0.306
0.35MetMet: 0.35 ± 0.18
0.839MetAsn: 0.839 ± 0.199
0.839MetPro: 0.839 ± 0.22
0.489MetGln: 0.489 ± 0.22
1.818MetArg: 1.818 ± 0.437
3.076MetSer: 3.076 ± 0.39
2.168MetThr: 2.168 ± 0.381
1.259MetVal: 1.259 ± 0.274
0.21MetTrp: 0.21 ± 0.126
0.559MetTyr: 0.559 ± 0.223
0.0MetXaa: 0.0 ± 0.0
Asn
3.076AsnAla: 3.076 ± 0.423
0.559AsnCys: 0.559 ± 0.238
2.168AsnAsp: 2.168 ± 0.409
2.028AsnGlu: 2.028 ± 0.366
1.119AsnPhe: 1.119 ± 0.251
3.566AsnGly: 3.566 ± 0.535
0.839AsnHis: 0.839 ± 0.214
1.328AsnIle: 1.328 ± 0.284
1.119AsnLys: 1.119 ± 0.275
3.076AsnLeu: 3.076 ± 0.383
0.979AsnMet: 0.979 ± 0.337
1.468AsnAsn: 1.468 ± 0.33
2.307AsnPro: 2.307 ± 0.355
0.699AsnGln: 0.699 ± 0.21
1.818AsnArg: 1.818 ± 0.389
2.237AsnSer: 2.237 ± 0.374
2.168AsnThr: 2.168 ± 0.564
2.447AsnVal: 2.447 ± 0.372
0.769AsnTrp: 0.769 ± 0.242
1.119AsnTyr: 1.119 ± 0.274
0.0AsnXaa: 0.0 ± 0.0
Pro
3.985ProAla: 3.985 ± 0.483
0.35ProCys: 0.35 ± 0.188
2.937ProAsp: 2.937 ± 0.325
3.496ProGlu: 3.496 ± 0.524
1.259ProPhe: 1.259 ± 0.239
4.335ProGly: 4.335 ± 0.553
0.699ProHis: 0.699 ± 0.2
2.587ProIle: 2.587 ± 0.46
1.468ProLys: 1.468 ± 0.282
3.846ProLeu: 3.846 ± 0.402
1.398ProMet: 1.398 ± 0.235
1.958ProAsn: 1.958 ± 0.438
1.398ProPro: 1.398 ± 0.273
1.608ProGln: 1.608 ± 0.29
2.797ProArg: 2.797 ± 0.537
2.587ProSer: 2.587 ± 0.417
3.846ProThr: 3.846 ± 0.562
4.195ProVal: 4.195 ± 0.582
1.119ProTrp: 1.119 ± 0.307
1.538ProTyr: 1.538 ± 0.272
0.0ProXaa: 0.0 ± 0.0
Gln
3.216GlnAla: 3.216 ± 0.471
0.28GlnCys: 0.28 ± 0.151
1.958GlnAsp: 1.958 ± 0.307
1.468GlnGlu: 1.468 ± 0.343
1.119GlnPhe: 1.119 ± 0.251
2.937GlnGly: 2.937 ± 0.604
0.629GlnHis: 0.629 ± 0.224
1.748GlnIle: 1.748 ± 0.269
0.699GlnLys: 0.699 ± 0.209
2.587GlnLeu: 2.587 ± 0.492
1.049GlnMet: 1.049 ± 0.258
0.769GlnAsn: 0.769 ± 0.199
1.818GlnPro: 1.818 ± 0.374
1.398GlnGln: 1.398 ± 0.307
3.076GlnArg: 3.076 ± 0.468
2.098GlnSer: 2.098 ± 0.33
1.608GlnThr: 1.608 ± 0.279
3.566GlnVal: 3.566 ± 0.501
0.699GlnTrp: 0.699 ± 0.213
0.909GlnTyr: 0.909 ± 0.281
0.0GlnXaa: 0.0 ± 0.0
Arg
5.174ArgAla: 5.174 ± 0.68
1.189ArgCys: 1.189 ± 0.332
4.335ArgAsp: 4.335 ± 0.568
4.894ArgGlu: 4.894 ± 0.736
2.377ArgPhe: 2.377 ± 0.416
4.055ArgGly: 4.055 ± 0.532
1.119ArgHis: 1.119 ± 0.399
2.797ArgIle: 2.797 ± 0.567
2.867ArgLys: 2.867 ± 0.489
5.524ArgLeu: 5.524 ± 0.606
0.979ArgMet: 0.979 ± 0.283
2.028ArgAsn: 2.028 ± 0.387
2.797ArgPro: 2.797 ± 0.419
2.237ArgGln: 2.237 ± 0.454
3.776ArgArg: 3.776 ± 0.513
3.566ArgSer: 3.566 ± 0.504
3.916ArgThr: 3.916 ± 0.621
4.755ArgVal: 4.755 ± 0.444
1.398ArgTrp: 1.398 ± 0.31
1.748ArgTyr: 1.748 ± 0.339
0.0ArgXaa: 0.0 ± 0.0
Ser
4.894SerAla: 4.894 ± 0.681
0.559SerCys: 0.559 ± 0.164
4.125SerAsp: 4.125 ± 0.403
4.405SerGlu: 4.405 ± 0.651
2.377SerPhe: 2.377 ± 0.42
5.803SerGly: 5.803 ± 0.812
1.119SerHis: 1.119 ± 0.269
2.307SerIle: 2.307 ± 0.407
2.377SerLys: 2.377 ± 0.455
5.594SerLeu: 5.594 ± 0.622
1.538SerMet: 1.538 ± 0.354
2.727SerAsn: 2.727 ± 0.439
2.447SerPro: 2.447 ± 0.488
1.818SerGln: 1.818 ± 0.369
3.706SerArg: 3.706 ± 0.528
3.776SerSer: 3.776 ± 0.594
3.496SerThr: 3.496 ± 0.402
4.894SerVal: 4.894 ± 0.62
1.189SerTrp: 1.189 ± 0.293
1.468SerTyr: 1.468 ± 0.321
0.0SerXaa: 0.0 ± 0.0
Thr
5.384ThrAla: 5.384 ± 0.637
0.42ThrCys: 0.42 ± 0.188
3.566ThrAsp: 3.566 ± 0.44
5.244ThrGlu: 5.244 ± 0.622
1.958ThrPhe: 1.958 ± 0.386
5.733ThrGly: 5.733 ± 0.737
1.119ThrHis: 1.119 ± 0.334
3.496ThrIle: 3.496 ± 0.5
2.797ThrLys: 2.797 ± 0.396
5.174ThrLeu: 5.174 ± 0.562
1.398ThrMet: 1.398 ± 0.249
1.748ThrAsn: 1.748 ± 0.362
3.985ThrPro: 3.985 ± 0.522
1.888ThrGln: 1.888 ± 0.297
3.356ThrArg: 3.356 ± 0.426
2.867ThrSer: 2.867 ± 0.366
3.706ThrThr: 3.706 ± 0.625
4.755ThrVal: 4.755 ± 0.529
1.049ThrTrp: 1.049 ± 0.225
2.587ThrTyr: 2.587 ± 0.385
0.0ThrXaa: 0.0 ± 0.0
Val
7.551ValAla: 7.551 ± 0.705
0.769ValCys: 0.769 ± 0.231
5.384ValAsp: 5.384 ± 0.62
6.293ValGlu: 6.293 ± 0.643
2.867ValPhe: 2.867 ± 0.45
5.664ValGly: 5.664 ± 0.717
1.189ValHis: 1.189 ± 0.291
4.125ValIle: 4.125 ± 0.539
3.076ValLys: 3.076 ± 0.502
6.852ValLeu: 6.852 ± 0.661
1.538ValMet: 1.538 ± 0.401
3.146ValAsn: 3.146 ± 0.379
3.706ValPro: 3.706 ± 0.466
2.517ValGln: 2.517 ± 0.416
4.545ValArg: 4.545 ± 0.534
5.314ValSer: 5.314 ± 0.506
4.685ValThr: 4.685 ± 0.576
6.503ValVal: 6.503 ± 0.916
1.259ValTrp: 1.259 ± 0.313
2.028ValTyr: 2.028 ± 0.452
0.0ValXaa: 0.0 ± 0.0
Trp
1.189TrpAla: 1.189 ± 0.319
0.0TrpCys: 0.0 ± 0.0
1.398TrpAsp: 1.398 ± 0.258
1.398TrpGlu: 1.398 ± 0.328
0.769TrpPhe: 0.769 ± 0.232
1.538TrpGly: 1.538 ± 0.341
0.559TrpHis: 0.559 ± 0.191
0.979TrpIle: 0.979 ± 0.246
0.839TrpLys: 0.839 ± 0.208
1.328TrpLeu: 1.328 ± 0.356
0.629TrpMet: 0.629 ± 0.166
0.839TrpAsn: 0.839 ± 0.254
0.629TrpPro: 0.629 ± 0.204
0.839TrpGln: 0.839 ± 0.234
0.559TrpArg: 0.559 ± 0.155
1.328TrpSer: 1.328 ± 0.313
1.398TrpThr: 1.398 ± 0.275
1.328TrpVal: 1.328 ± 0.244
0.35TrpTrp: 0.35 ± 0.173
0.629TrpTyr: 0.629 ± 0.235
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.286TyrAla: 3.286 ± 0.509
0.14TyrCys: 0.14 ± 0.094
2.727TyrAsp: 2.727 ± 0.423
2.587TyrGlu: 2.587 ± 0.455
0.839TyrPhe: 0.839 ± 0.251
2.937TyrGly: 2.937 ± 0.62
0.489TyrHis: 0.489 ± 0.174
1.678TyrIle: 1.678 ± 0.321
0.489TyrLys: 0.489 ± 0.203
2.447TyrLeu: 2.447 ± 0.44
1.049TyrMet: 1.049 ± 0.228
1.328TyrAsn: 1.328 ± 0.296
0.629TyrPro: 0.629 ± 0.203
1.189TyrGln: 1.189 ± 0.227
2.867TyrArg: 2.867 ± 0.5
2.098TyrSer: 2.098 ± 0.369
1.328TyrThr: 1.328 ± 0.318
2.028TyrVal: 2.028 ± 0.383
0.21TyrTrp: 0.21 ± 0.113
0.629TyrTyr: 0.629 ± 0.24
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (14303 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski