Phage AS32

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C1K533|A0A5C1K533_9CAUD Exonuclease OS=Phage AS32 OX=2604894 PE=4 SV=1
MM1 pKa = 7.73PSVNDD6 pKa = 3.52NFADD10 pKa = 4.63AIEE13 pKa = 4.15VQIATNGGTYY23 pKa = 9.71TSPSLDD29 pKa = 3.33TAGNTGEE36 pKa = 4.18VGEE39 pKa = 4.12PQIAGWEE46 pKa = 4.17VSAWWKK52 pKa = 7.12YY53 pKa = 7.96TPSSSGNATFDD64 pKa = 3.83TQLSTPTTTGTDD76 pKa = 3.26TYY78 pKa = 11.38VAIHH82 pKa = 6.75TGTALNNLVQVAADD96 pKa = 4.13DD97 pKa = 4.33DD98 pKa = 4.78SGGSRR103 pKa = 11.84TSLISNLAVTAGEE116 pKa = 4.46TYY118 pKa = 9.69WIQVGGFGTQTMNLVLRR135 pKa = 11.84VTGPASGTGGSGGNANAVPATASAQVIPPTVTGSSQGAEE174 pKa = 4.2VAAVPATATAQAIPAASISGGTIVEE199 pKa = 4.47GAGPATATAQVLLPTVTGEE218 pKa = 4.17SGSTGGNANAVPAIATAITLAPAVSAGSDD247 pKa = 2.91IDD249 pKa = 4.36AVPATAVAQSNLPAVSTGSDD269 pKa = 3.13VDD271 pKa = 4.0AVPATATAAALAPVVTGSSSGGGSDD296 pKa = 4.7QGFLNAWYY304 pKa = 10.17SDD306 pKa = 3.19GVTWTQII313 pKa = 3.1

Molecular weight: 30.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C1K588|A0A5C1K588_9CAUD M15 family peptidase OS=Phage AS32 OX=2604894 PE=4 SV=1
MM1 pKa = 6.93TFKK4 pKa = 11.01RR5 pKa = 11.84LLTDD9 pKa = 3.28EE10 pKa = 4.48QEE12 pKa = 4.28AEE14 pKa = 4.2LVKK17 pKa = 10.59EE18 pKa = 3.99YY19 pKa = 11.0LGVDD23 pKa = 2.88DD24 pKa = 3.75WTINEE29 pKa = 4.32LSVMFKK35 pKa = 10.22VSKK38 pKa = 8.67RR39 pKa = 11.84TVWRR43 pKa = 11.84ILQKK47 pKa = 10.71HH48 pKa = 5.02NARR51 pKa = 11.84RR52 pKa = 11.84PPLKK56 pKa = 9.86HH57 pKa = 6.13HH58 pKa = 6.85KK59 pKa = 8.84KK60 pKa = 8.19TAFKK64 pKa = 9.89LQRR67 pKa = 11.84VKK69 pKa = 10.59PSKK72 pKa = 10.65VKK74 pKa = 9.89PKK76 pKa = 10.27KK77 pKa = 10.0KK78 pKa = 10.12RR79 pKa = 11.84EE80 pKa = 3.93LLPCGTNAAYY90 pKa = 9.87HH91 pKa = 5.44RR92 pKa = 11.84HH93 pKa = 5.68RR94 pKa = 11.84KK95 pKa = 8.81AGEE98 pKa = 4.16YY99 pKa = 10.1PCTDD103 pKa = 3.56CLAAHH108 pKa = 7.16AEE110 pKa = 4.1NVKK113 pKa = 10.02LAKK116 pKa = 9.65QKK118 pKa = 10.22RR119 pKa = 11.84KK120 pKa = 9.92KK121 pKa = 10.32RR122 pKa = 11.84EE123 pKa = 3.71QTRR126 pKa = 11.84SSDD129 pKa = 3.35LARR132 pKa = 4.95

Molecular weight: 15.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

45

0

45

11956

53

2022

265.7

29.29

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.259 ± 0.58

0.569 ± 0.13

6.156 ± 0.3

6.633 ± 0.506

3.697 ± 0.183

8.029 ± 0.365

1.439 ± 0.125

5.671 ± 0.249

5.094 ± 0.449

7.653 ± 0.452

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.434 ± 0.238

4.508 ± 0.208

4.701 ± 0.37

3.772 ± 0.189

5.077 ± 0.269

6.566 ± 0.505

6.524 ± 0.53

7.185 ± 0.368

1.974 ± 0.219

3.061 ± 0.266
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski