Thioalkalivibrio sp. (strain K90mix)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Chromatiales; Ectothiorhodospiraceae; Thioalkalivibrio; unclassified Thioalkalivibrio

Average proteome isoelectric point is 5.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2823 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D3S8T4|D3S8T4_THISK Response regulator receiver modulated CheW protein OS=Thioalkalivibrio sp. (strain K90mix) OX=396595 GN=TK90_0924 PE=4 SV=1
MM1 pKa = 7.38FSLIIVLISIGLAAALAVATLYY23 pKa = 11.12YY24 pKa = 10.71GGDD27 pKa = 3.54VFVGEE32 pKa = 4.39SANAEE37 pKa = 4.06SARR40 pKa = 11.84ILNEE44 pKa = 3.25ATQIVGAVNLRR55 pKa = 11.84SGRR58 pKa = 11.84EE59 pKa = 3.96GTLITDD65 pKa = 3.73MNEE68 pKa = 4.02DD69 pKa = 3.67LVPRR73 pKa = 11.84YY74 pKa = 9.42IQTVPEE80 pKa = 4.06GWVIDD85 pKa = 4.03EE86 pKa = 4.45NEE88 pKa = 4.1GVIYY92 pKa = 10.64LPGDD96 pKa = 3.63AVSDD100 pKa = 4.04AACEE104 pKa = 4.0RR105 pKa = 11.84MNEE108 pKa = 4.1RR109 pKa = 11.84QGADD113 pKa = 3.0HH114 pKa = 6.96TSFDD118 pKa = 4.18SVGSEE123 pKa = 3.8DD124 pKa = 4.76RR125 pKa = 11.84LVPSCDD131 pKa = 3.45DD132 pKa = 4.07EE133 pKa = 6.95GMDD136 pKa = 5.21DD137 pKa = 5.22YY138 pKa = 11.6PAICCTNDD146 pKa = 2.61AA147 pKa = 4.63

Molecular weight: 15.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D3SFI1|D3SFI1_THISK Uncharacterized protein OS=Thioalkalivibrio sp. (strain K90mix) OX=396595 GN=TK90_0696 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.8GFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 8.96VLNARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.75GRR39 pKa = 11.84KK40 pKa = 8.72RR41 pKa = 11.84LCPTNN46 pKa = 3.52

Molecular weight: 5.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2823

0

2823

877254

34

2202

310.8

34.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.318 ± 0.062

0.822 ± 0.014

6.276 ± 0.037

7.232 ± 0.05

3.309 ± 0.028

8.348 ± 0.042

2.493 ± 0.028

4.564 ± 0.031

2.239 ± 0.044

10.739 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.021

2.404 ± 0.027

5.306 ± 0.037

3.739 ± 0.027

8.196 ± 0.05

4.793 ± 0.03

4.89 ± 0.024

7.289 ± 0.039

1.426 ± 0.02

2.237 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski