Megaselia scalaris (Humpbacked fly) (Phora scalaris)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Eremoneura; Cyclorrhapha; Aschiza; Platypezoidea;

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11466 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T1GE44|T1GE44_MEGSC Uncharacterized protein OS=Megaselia scalaris OX=36166 PE=3 SV=1
MM1 pKa = 7.21SLCGGMEE8 pKa = 3.86KK9 pKa = 10.58GGPTDD14 pKa = 4.43EE15 pKa = 5.85EE16 pKa = 4.13FDD18 pKa = 3.69QNIIKK23 pKa = 10.84YY24 pKa = 10.4SDD26 pKa = 3.43VCEE29 pKa = 4.23NPSLFSSPNLVVRR42 pKa = 11.84LNGKK46 pKa = 9.17YY47 pKa = 9.95YY48 pKa = 10.1SWSAACPIVMTIITFQKK65 pKa = 10.21PLTNKK70 pKa = 8.7KK71 pKa = 8.78TDD73 pKa = 3.1VFDD76 pKa = 6.12DD77 pKa = 4.89DD78 pKa = 5.87DD79 pKa = 5.76VNDD82 pKa = 5.54DD83 pKa = 5.57DD84 pKa = 7.27DD85 pKa = 7.61DD86 pKa = 6.63DD87 pKa = 6.98DD88 pKa = 5.0EE89 pKa = 5.53LQFSCSSDD97 pKa = 3.28NDD99 pKa = 3.43DD100 pKa = 5.1DD101 pKa = 5.45YY102 pKa = 12.23NVASGNDD109 pKa = 3.6DD110 pKa = 4.01DD111 pKa = 5.92NKK113 pKa = 10.76LGYY116 pKa = 10.37DD117 pKa = 3.87FYY119 pKa = 11.75FFLL122 pKa = 6.25

Molecular weight: 13.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T1H7E3|T1H7E3_MEGSC Ubiquitinyl hydrolase 1 OS=Megaselia scalaris OX=36166 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.12WRR6 pKa = 11.84KK7 pKa = 9.1KK8 pKa = 9.32RR9 pKa = 11.84MRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.92RR15 pKa = 11.84KK16 pKa = 6.92RR17 pKa = 11.84WKK19 pKa = 9.78MRR21 pKa = 11.84VHH23 pKa = 6.77SKK25 pKa = 10.8

Molecular weight: 3.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11461

5

11466

2583012

11

4054

225.3

24.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.247 ± 0.024

1.961 ± 0.025

5.137 ± 0.023

6.443 ± 0.039

4.342 ± 0.022

5.316 ± 0.034

2.333 ± 0.015

6.511 ± 0.025

7.031 ± 0.032

8.687 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.015

5.748 ± 0.027

4.719 ± 0.03

4.073 ± 0.028

4.491 ± 0.024

8.08 ± 0.044

5.55 ± 0.029

5.631 ± 0.023

1.025 ± 0.01

3.295 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski