Gracilibacillus boraciitolerans JCM 21714

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Gracilibacillus; Gracilibacillus boraciitolerans

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4450 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4VMD6|W4VMD6_9BACI Oxidoreductase OS=Gracilibacillus boraciitolerans JCM 21714 OX=1298598 GN=JCM21714_3508 PE=4 SV=1
MM1 pKa = 6.95KK2 pKa = 10.61QNGFSYY8 pKa = 10.6DD9 pKa = 3.41YY10 pKa = 11.02VNAVEE15 pKa = 4.46MPPAEE20 pKa = 4.2MPAALSEE27 pKa = 3.99GRR29 pKa = 11.84IAGYY33 pKa = 10.09VVAEE37 pKa = 4.12PFGAQSVVHH46 pKa = 6.26GNGKK50 pKa = 9.06VLYY53 pKa = 10.52QEE55 pKa = 4.69DD56 pKa = 5.07DD57 pKa = 3.35LWKK60 pKa = 10.72DD61 pKa = 3.93AIDD64 pKa = 3.82CALVLRR70 pKa = 11.84TEE72 pKa = 5.49FINEE76 pKa = 3.75QQTAAEE82 pKa = 4.41EE83 pKa = 4.15FVNAYY88 pKa = 9.85VDD90 pKa = 3.57AGLKK94 pKa = 10.38AEE96 pKa = 4.63EE97 pKa = 4.39GHH99 pKa = 6.53EE100 pKa = 4.12EE101 pKa = 4.05TNQIIQDD108 pKa = 3.9YY109 pKa = 10.52LDD111 pKa = 3.82VDD113 pKa = 4.82DD114 pKa = 5.61EE115 pKa = 4.54VLDD118 pKa = 5.86LSLEE122 pKa = 4.27WISYY126 pKa = 11.11DD127 pKa = 4.66DD128 pKa = 4.89LKK130 pKa = 11.07INQDD134 pKa = 2.93SYY136 pKa = 11.03TEE138 pKa = 3.8LRR140 pKa = 11.84EE141 pKa = 4.07YY142 pKa = 9.93IIEE145 pKa = 4.33MGLSEE150 pKa = 5.28NPPTYY155 pKa = 10.78DD156 pKa = 3.08EE157 pKa = 5.16FVDD160 pKa = 4.32NSLFDD165 pKa = 3.59KK166 pKa = 11.34AMGSNEE172 pKa = 3.68

Molecular weight: 19.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4VKF1|W4VKF1_9BACI Transport permease protein OS=Gracilibacillus boraciitolerans JCM 21714 OX=1298598 GN=JCM21714_2369 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.22KK14 pKa = 8.69VHH16 pKa = 5.46GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.36VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4450

0

4450

993814

37

1371

223.3

25.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.416 ± 0.042

0.655 ± 0.012

5.491 ± 0.029

7.559 ± 0.048

4.526 ± 0.034

6.36 ± 0.043

2.158 ± 0.018

8.474 ± 0.04

6.829 ± 0.037

9.512 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.794 ± 0.019

4.927 ± 0.031

3.409 ± 0.02

4.303 ± 0.031

3.853 ± 0.03

5.919 ± 0.026

5.425 ± 0.028

6.532 ± 0.03

1.079 ± 0.015

3.777 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski