Nitrosomonas sp. Nm166

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas; unclassified Nitrosomonas

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3014 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I2CYB0|A0A1I2CYB0_9PROT 2-dehydro-3-deoxyglucarate aldolase OS=Nitrosomonas sp. Nm166 OX=1881054 GN=SAMN05428977_102718 PE=4 SV=1
MM1 pKa = 7.4ATGQEE6 pKa = 3.92VLAYY10 pKa = 10.58LGLTVEE16 pKa = 4.37QANKK20 pKa = 10.01FIQEE24 pKa = 4.21NIGQPEE30 pKa = 4.45IIFDD34 pKa = 3.58AAYY37 pKa = 10.44DD38 pKa = 3.75VGATTQHH45 pKa = 6.51LSDD48 pKa = 3.09ITGYY52 pKa = 8.44STDD55 pKa = 3.58TIKK58 pKa = 11.18NFFAAFEE65 pKa = 4.94LDD67 pKa = 3.33TKK69 pKa = 11.29LLDD72 pKa = 3.54EE73 pKa = 4.56VKK75 pKa = 10.45ILFNSEE81 pKa = 4.1LGDD84 pKa = 3.49LANFVGFNDD93 pKa = 3.45HH94 pKa = 7.02SGVLSTASLGDD105 pKa = 3.68QVKK108 pKa = 10.84LDD110 pKa = 4.11VDD112 pKa = 3.75SSDD115 pKa = 3.18YY116 pKa = 11.41SFFYY120 pKa = 10.95EE121 pKa = 4.9PVFDD125 pKa = 3.99YY126 pKa = 11.03QQADD130 pKa = 4.47GIYY133 pKa = 8.96TPDD136 pKa = 3.44EE137 pKa = 4.69LGVSHH142 pKa = 7.39LGNVPATDD150 pKa = 3.71EE151 pKa = 4.65SIEE154 pKa = 4.2SLVYY158 pKa = 9.1GTLINIYY165 pKa = 9.88SALDD169 pKa = 3.52EE170 pKa = 4.72TEE172 pKa = 4.54LSQLRR177 pKa = 11.84GFSHH181 pKa = 6.83NEE183 pKa = 3.82SNVNEE188 pKa = 4.05YY189 pKa = 10.97RR190 pKa = 11.84SMLIDD195 pKa = 4.33ALSDD199 pKa = 3.3PANRR203 pKa = 11.84SDD205 pKa = 4.86QDD207 pKa = 3.88LADD210 pKa = 5.24LIVDD214 pKa = 3.72EE215 pKa = 4.53TVILIDD221 pKa = 4.32EE222 pKa = 4.62YY223 pKa = 10.32WNNMDD228 pKa = 3.04VTGILDD234 pKa = 3.48HH235 pKa = 7.19SILGLAGIAA244 pKa = 3.84

Molecular weight: 26.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I2H9X1|A0A1I2H9X1_9PROT Uncharacterized protein OS=Nitrosomonas sp. Nm166 OX=1881054 GN=SAMN05428977_10931 PE=4 SV=1
MM1 pKa = 6.64QCKK4 pKa = 9.45LARR7 pKa = 11.84WSSEE11 pKa = 3.53DD12 pKa = 3.46KK13 pKa = 10.39EE14 pKa = 4.61RR15 pKa = 11.84KK16 pKa = 8.9FDD18 pKa = 3.57RR19 pKa = 11.84LLRR22 pKa = 11.84VIANRR27 pKa = 11.84SWLQQAARR35 pKa = 11.84VTLASNGAKK44 pKa = 9.48TPGVDD49 pKa = 4.37NIDD52 pKa = 3.29KK53 pKa = 10.71RR54 pKa = 11.84AMEE57 pKa = 4.81RR58 pKa = 11.84NLQHH62 pKa = 5.66QLEE65 pKa = 4.59MIRR68 pKa = 11.84CEE70 pKa = 4.08LLAGNYY76 pKa = 8.45QPQPARR82 pKa = 11.84RR83 pKa = 11.84VYY85 pKa = 10.1IPKK88 pKa = 10.52ANGKK92 pKa = 8.0QRR94 pKa = 11.84PLGIPALRR102 pKa = 11.84DD103 pKa = 3.6RR104 pKa = 11.84IVQRR108 pKa = 11.84AMQMAMEE115 pKa = 5.62PIWEE119 pKa = 4.29SDD121 pKa = 3.31FHH123 pKa = 6.82RR124 pKa = 11.84LSYY127 pKa = 10.7GFRR130 pKa = 11.84PEE132 pKa = 4.16RR133 pKa = 11.84SVHH136 pKa = 4.83HH137 pKa = 7.49AIRR140 pKa = 11.84TVKK143 pKa = 10.45FQLQDD148 pKa = 3.32STNTSGRR155 pKa = 11.84WVIEE159 pKa = 3.83GDD161 pKa = 3.22LASYY165 pKa = 10.84FDD167 pKa = 4.27TVHH170 pKa = 6.74HH171 pKa = 6.6KK172 pKa = 11.09LLMKK176 pKa = 9.94CVRR179 pKa = 11.84RR180 pKa = 11.84RR181 pKa = 11.84IRR183 pKa = 11.84DD184 pKa = 3.43TRR186 pKa = 11.84FLSLLWRR193 pKa = 11.84FIKK196 pKa = 10.49AGHH199 pKa = 5.95VDD201 pKa = 3.13QGLFQAASNGVPQGGVISPLLSNIMLTMVTSSVII235 pKa = 3.3

Molecular weight: 27.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3014

0

3014

915179

25

3005

303.6

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.942 ± 0.053

0.987 ± 0.017

5.454 ± 0.053

5.932 ± 0.045

4.071 ± 0.033

7.091 ± 0.067

2.476 ± 0.025

6.941 ± 0.041

4.793 ± 0.048

10.456 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.394 ± 0.026

4.245 ± 0.036

4.325 ± 0.036

4.323 ± 0.035

5.429 ± 0.038

6.293 ± 0.043

5.33 ± 0.05

6.389 ± 0.041

1.263 ± 0.021

2.865 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski