NL63-related bat coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Setracovirus; NL63-related bat coronavirus strain BtKYNL63-9b

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L2KGB5|A0A1L2KGB5_9ALPC Isoform of A0A1L2KGB4 3C-like proteinase OS=NL63-related bat coronavirus OX=1920748 GN=orf1ab PE=3 SV=1
MM1 pKa = 8.03DD2 pKa = 5.11FMLFLYY8 pKa = 10.53ALSLFIEE15 pKa = 4.63EE16 pKa = 4.59GLPVAIALGVWAAEE30 pKa = 4.27VTGLVYY36 pKa = 10.2FLYY39 pKa = 10.76VDD41 pKa = 3.98GPVVLFTWNFLICYY55 pKa = 8.96AFLYY59 pKa = 9.91FILVPLVQEE68 pKa = 4.47LFLPGALDD76 pKa = 3.78LALDD80 pKa = 3.84QLRR83 pKa = 11.84GFHH86 pKa = 6.66DD87 pKa = 4.7FIVRR91 pKa = 11.84AMFCC95 pKa = 3.79

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L2KGC0|A0A1L2KGC0_9ALPC Membrane protein OS=NL63-related bat coronavirus OX=1920748 GN=M PE=3 SV=1
MM1 pKa = 7.26ATVNWADD8 pKa = 3.82DD9 pKa = 3.51KK10 pKa = 11.1RR11 pKa = 11.84GKK13 pKa = 9.97RR14 pKa = 11.84KK15 pKa = 9.77YY16 pKa = 9.36PPPSFYY22 pKa = 11.06LPLVVKK28 pKa = 10.43SDD30 pKa = 3.34KK31 pKa = 10.59QPYY34 pKa = 9.07KK35 pKa = 10.55VIPRR39 pKa = 11.84NLVPKK44 pKa = 10.77GKK46 pKa = 10.37GNKK49 pKa = 8.87DD50 pKa = 3.28QQIGYY55 pKa = 9.33WNVQRR60 pKa = 11.84RR61 pKa = 11.84WRR63 pKa = 11.84MRR65 pKa = 11.84KK66 pKa = 7.78GQRR69 pKa = 11.84VDD71 pKa = 3.01IDD73 pKa = 4.02PKK75 pKa = 9.33VHH77 pKa = 7.15FYY79 pKa = 11.42YY80 pKa = 10.81LGTGPRR86 pKa = 11.84ADD88 pKa = 3.08LKK90 pKa = 10.72FRR92 pKa = 11.84EE93 pKa = 4.26RR94 pKa = 11.84AEE96 pKa = 4.14DD97 pKa = 3.93VVWVAMQGSKK107 pKa = 9.31TEE109 pKa = 4.0PTNLGNRR116 pKa = 11.84KK117 pKa = 9.39RR118 pKa = 11.84NQKK121 pKa = 9.76PIQPEE126 pKa = 3.58FDD128 pKa = 2.91IQLPNEE134 pKa = 4.15LEE136 pKa = 4.17VVEE139 pKa = 5.57FEE141 pKa = 4.89DD142 pKa = 4.91RR143 pKa = 11.84SNSSSRR149 pKa = 11.84ASSRR153 pKa = 11.84ASSRR157 pKa = 11.84GNSRR161 pKa = 11.84EE162 pKa = 3.71TSRR165 pKa = 11.84SNSRR169 pKa = 11.84QQSRR173 pKa = 11.84DD174 pKa = 2.95NSRR177 pKa = 11.84SPSRR181 pKa = 11.84SRR183 pKa = 11.84SNSTSEE189 pKa = 3.96SSQNSAQDD197 pKa = 3.11LVAAVTAALKK207 pKa = 10.74NLGFEE212 pKa = 4.48PPKK215 pKa = 10.38SDD217 pKa = 3.67KK218 pKa = 11.12SGNASGTSTPKK229 pKa = 10.57GKK231 pKa = 10.35KK232 pKa = 9.32KK233 pKa = 10.24PKK235 pKa = 8.18QAKK238 pKa = 9.82SNEE241 pKa = 4.0QGSPNNVPSDD251 pKa = 3.34KK252 pKa = 11.25SQMNKK257 pKa = 9.89PKK259 pKa = 10.06WKK261 pKa = 9.61RR262 pKa = 11.84VPNASEE268 pKa = 4.32NVIKK272 pKa = 10.87CFGPRR277 pKa = 11.84DD278 pKa = 3.61FDD280 pKa = 5.02HH281 pKa = 7.2NMGDD285 pKa = 3.7ADD287 pKa = 4.08LVQNGVEE294 pKa = 4.3AKK296 pKa = 10.2NFPQIAEE303 pKa = 5.01LIPTQAAMFFDD314 pKa = 4.43SEE316 pKa = 4.57VSTKK320 pKa = 10.8EE321 pKa = 3.64MGNKK325 pKa = 8.67VQIIYY330 pKa = 8.58TYY332 pKa = 11.34KK333 pKa = 10.23MLVDD337 pKa = 4.6KK338 pKa = 10.89DD339 pKa = 3.9NKK341 pKa = 10.15HH342 pKa = 5.78LPKK345 pKa = 10.47FLEE348 pKa = 4.12QVSAFTKK355 pKa = 9.5PSVVKK360 pKa = 8.77EE361 pKa = 4.49TQSHH365 pKa = 6.79PLQNTMPEE373 pKa = 4.05PAQLNVAAAEE383 pKa = 4.14FKK385 pKa = 11.01PPVTTASDD393 pKa = 3.55GSNAEE398 pKa = 3.77IEE400 pKa = 4.33IVDD403 pKa = 4.18EE404 pKa = 4.24VLHH407 pKa = 6.31

Molecular weight: 45.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

1

8

13252

77

6758

1656.5

184.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.807 ± 0.247

3.192 ± 0.266

5.652 ± 0.335

3.894 ± 0.225

5.886 ± 0.35

6.58 ± 0.346

1.637 ± 0.104

4.694 ± 0.375

5.946 ± 0.494

9.417 ± 0.695

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.932 ± 0.189

5.516 ± 0.255

3.63 ± 0.223

2.92 ± 0.43

3.441 ± 0.205

7.516 ± 0.553

5.75 ± 0.411

9.931 ± 0.355

1.268 ± 0.193

4.377 ± 0.262
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski