Ourmia melon virus (isolate Melon/Iran/VE9) (OuMV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Miaviricetes; Ourlivirales; Botourmiaviridae; Ourmiavirus; Ourmia melon virus

Average proteome isoelectric point is 8.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|B3VML2|MVP_OUMVV Putative movement protein OS=Ourmia melon virus (isolate Melon/Iran/VE9) OX=652838 PE=2 SV=1
MM1 pKa = 7.82EE2 pKa = 5.55PRR4 pKa = 11.84QEE6 pKa = 4.18LLDD9 pKa = 3.7PEE11 pKa = 4.51RR12 pKa = 11.84VRR14 pKa = 11.84EE15 pKa = 3.8EE16 pKa = 3.53AKK18 pKa = 10.68RR19 pKa = 11.84IVRR22 pKa = 11.84WLCGLVDD29 pKa = 3.87RR30 pKa = 11.84TGKK33 pKa = 10.53LPAGDD38 pKa = 3.86YY39 pKa = 9.82QGKK42 pKa = 9.24ILNTVSEE49 pKa = 3.96ICKK52 pKa = 10.04RR53 pKa = 11.84SKK55 pKa = 10.75LPCGEE60 pKa = 4.03LAEE63 pKa = 4.7ALTKK67 pKa = 10.76GRR69 pKa = 11.84LTRR72 pKa = 11.84SLVDD76 pKa = 3.39YY77 pKa = 11.37SEE79 pKa = 5.24LLISNLVVGYY89 pKa = 10.15FDD91 pKa = 3.61VLEE94 pKa = 4.69IYY96 pKa = 10.31LGKK99 pKa = 10.46QSVRR103 pKa = 11.84LSDD106 pKa = 3.45IRR108 pKa = 11.84EE109 pKa = 4.05MACKK113 pKa = 9.06YY114 pKa = 8.81TFYY117 pKa = 10.79AINRR121 pKa = 11.84RR122 pKa = 11.84LEE124 pKa = 4.3DD125 pKa = 3.67YY126 pKa = 11.03IKK128 pKa = 10.78FQTAWLQARR137 pKa = 11.84AMRR140 pKa = 11.84DD141 pKa = 3.31VTPEE145 pKa = 3.77PEE147 pKa = 4.2APSWLNEE154 pKa = 3.6GFGRR158 pKa = 11.84CFSALLNRR166 pKa = 11.84KK167 pKa = 7.83VHH169 pKa = 5.78LRR171 pKa = 11.84CVLRR175 pKa = 11.84ARR177 pKa = 11.84PNDD180 pKa = 3.3VSLAASLYY188 pKa = 7.41QVKK191 pKa = 9.61RR192 pKa = 11.84VAPPLPDD199 pKa = 3.67DD200 pKa = 4.12QIEE203 pKa = 4.33KK204 pKa = 10.58NLEE207 pKa = 3.79KK208 pKa = 11.01SLDD211 pKa = 3.62RR212 pKa = 11.84LTKK215 pKa = 10.31DD216 pKa = 3.47EE217 pKa = 4.59EE218 pKa = 4.26PAGVDD223 pKa = 3.53EE224 pKa = 5.91PFLEE228 pKa = 4.44DD229 pKa = 5.08LKK231 pKa = 11.02RR232 pKa = 11.84EE233 pKa = 4.37CKK235 pKa = 9.35RR236 pKa = 11.84TVDD239 pKa = 3.8EE240 pKa = 4.41LVQNARR246 pKa = 11.84RR247 pKa = 11.84EE248 pKa = 4.0GWNRR252 pKa = 11.84KK253 pKa = 7.82ISRR256 pKa = 11.84DD257 pKa = 3.73CFPSQSAAFEE267 pKa = 4.21NPISKK272 pKa = 10.31GGQLGQLVKK281 pKa = 10.9EE282 pKa = 4.43NNTPRR287 pKa = 11.84LPVLLGMFEE296 pKa = 4.18YY297 pKa = 10.19KK298 pKa = 10.76GRR300 pKa = 11.84VTPVYY305 pKa = 10.46GWADD309 pKa = 3.78DD310 pKa = 3.84GDD312 pKa = 4.49TILSDD317 pKa = 3.64EE318 pKa = 4.24EE319 pKa = 4.22LGRR322 pKa = 11.84EE323 pKa = 4.01VPAALKK329 pKa = 10.31CRR331 pKa = 11.84RR332 pKa = 11.84SPVLEE337 pKa = 4.0PFKK340 pKa = 11.18VRR342 pKa = 11.84VITMGPAVQYY352 pKa = 9.61YY353 pKa = 8.97RR354 pKa = 11.84ARR356 pKa = 11.84RR357 pKa = 11.84VQGCLWDD364 pKa = 4.06LLKK367 pKa = 9.76HH368 pKa = 5.33TRR370 pKa = 11.84CTHH373 pKa = 5.7LPNRR377 pKa = 11.84PVEE380 pKa = 3.97EE381 pKa = 4.15SDD383 pKa = 2.7IGFYY387 pKa = 9.64VRR389 pKa = 11.84RR390 pKa = 11.84RR391 pKa = 11.84GADD394 pKa = 3.14LFRR397 pKa = 11.84GEE399 pKa = 4.25EE400 pKa = 4.09VPYY403 pKa = 11.08VSGDD407 pKa = 3.4YY408 pKa = 10.76SAATDD413 pKa = 3.91NLHH416 pKa = 7.25PDD418 pKa = 3.57LSLSVVDD425 pKa = 4.89RR426 pKa = 11.84VCDD429 pKa = 3.86HH430 pKa = 6.93LLSDD434 pKa = 5.28DD435 pKa = 4.35NRR437 pKa = 11.84PLDD440 pKa = 3.99PVSPWRR446 pKa = 11.84VLFHH450 pKa = 6.57RR451 pKa = 11.84VLVGHH456 pKa = 7.54RR457 pKa = 11.84IYY459 pKa = 11.08DD460 pKa = 3.89GNSSRR465 pKa = 11.84NTEE468 pKa = 3.65VAAQSWGQLMGSPLSFPVLCIVNLAVTRR496 pKa = 11.84YY497 pKa = 9.37VLEE500 pKa = 4.32KK501 pKa = 9.58ACGRR505 pKa = 11.84IVTLEE510 pKa = 3.71EE511 pKa = 3.98SGILVNGDD519 pKa = 4.36DD520 pKa = 3.68ILFRR524 pKa = 11.84CPEE527 pKa = 3.81RR528 pKa = 11.84TIPFWTRR535 pKa = 11.84MVTIAGLSPSPGKK548 pKa = 10.43NFVSYY553 pKa = 9.85RR554 pKa = 11.84YY555 pKa = 9.28CQLNSEE561 pKa = 4.9LYY563 pKa = 10.88DD564 pKa = 3.49MSGSRR569 pKa = 11.84AEE571 pKa = 3.9YY572 pKa = 10.78LPFIKK577 pKa = 10.62ANLIYY582 pKa = 9.25GTLARR587 pKa = 11.84GCEE590 pKa = 4.04RR591 pKa = 11.84KK592 pKa = 9.52RR593 pKa = 11.84AADD596 pKa = 3.62LCYY599 pKa = 10.9GDD601 pKa = 3.79TTTEE605 pKa = 3.99GGTFGHH611 pKa = 7.55RR612 pKa = 11.84ARR614 pKa = 11.84ALIKK618 pKa = 10.86GFGPDD623 pKa = 3.11MQDD626 pKa = 2.89RR627 pKa = 11.84LMSRR631 pKa = 11.84FLHH634 pKa = 6.08SIKK637 pKa = 10.69GFLEE641 pKa = 4.55KK642 pKa = 10.05IPEE645 pKa = 4.27VSWFIHH651 pKa = 5.46PRR653 pKa = 11.84YY654 pKa = 9.95GGLGLPLTRR663 pKa = 11.84PVTHH667 pKa = 6.48NPYY670 pKa = 9.89HH671 pKa = 6.24LRR673 pKa = 11.84IAAYY677 pKa = 9.05LSCGGEE683 pKa = 3.89QSQEE687 pKa = 3.97ARR689 pKa = 11.84CMMQWLSAPTKK700 pKa = 10.12SFNAATLLRR709 pKa = 11.84ILEE712 pKa = 4.1VARR715 pKa = 11.84NCKK718 pKa = 9.93VPFRR722 pKa = 11.84KK723 pKa = 9.99VPFALLHH730 pKa = 5.59RR731 pKa = 11.84AEE733 pKa = 4.28AAGVDD738 pKa = 3.98LEE740 pKa = 4.22ALFRR744 pKa = 11.84KK745 pKa = 9.81ALLRR749 pKa = 11.84SAPRR753 pKa = 11.84LGVEE757 pKa = 4.25YY758 pKa = 9.68PSNEE762 pKa = 4.07SGDD765 pKa = 3.82MQRR768 pKa = 11.84LGDD771 pKa = 3.47WRR773 pKa = 11.84RR774 pKa = 11.84FFRR777 pKa = 11.84DD778 pKa = 3.06VGRR781 pKa = 11.84KK782 pKa = 8.27AARR785 pKa = 11.84TRR787 pKa = 11.84CRR789 pKa = 11.84SGTADD794 pKa = 3.2EE795 pKa = 4.51RR796 pKa = 11.84KK797 pKa = 10.28GLFLMSPDD805 pKa = 3.64NAVKK809 pKa = 10.43GPQYY813 pKa = 10.98EE814 pKa = 4.97YY815 pKa = 10.4IFDD818 pKa = 3.98WASHH822 pKa = 4.79NMGGNIWDD830 pKa = 3.72PSYY833 pKa = 11.38KK834 pKa = 10.02FRR836 pKa = 11.84ADD838 pKa = 3.36PFSSSEE844 pKa = 3.92SDD846 pKa = 3.09EE847 pKa = 4.18PRR849 pKa = 11.84AKK851 pKa = 10.49EE852 pKa = 3.52IGPNRR857 pKa = 11.84GPEE860 pKa = 3.7

Molecular weight: 97.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B3VML3|CAPSD_OUMVV Capsid protein OS=Ourmia melon virus (isolate Melon/Iran/VE9) OX=652838 PE=2 SV=1
MM1 pKa = 7.55ARR3 pKa = 11.84LPKK6 pKa = 10.25RR7 pKa = 11.84KK8 pKa = 9.26NRR10 pKa = 11.84RR11 pKa = 11.84NEE13 pKa = 3.75KK14 pKa = 10.12KK15 pKa = 10.5KK16 pKa = 10.1NANASRR22 pKa = 11.84VQNVPRR28 pKa = 11.84TFGLWKK34 pKa = 7.82STEE37 pKa = 4.11RR38 pKa = 11.84IKK40 pKa = 10.73YY41 pKa = 4.59TTEE44 pKa = 3.44LKK46 pKa = 10.75YY47 pKa = 11.19LNSKK51 pKa = 9.58CRR53 pKa = 11.84AIRR56 pKa = 11.84LHH58 pKa = 7.11PDD60 pKa = 3.55LVANNSFPTYY70 pKa = 10.0CSAWKK75 pKa = 9.49IDD77 pKa = 3.42QVEE80 pKa = 4.4FEE82 pKa = 4.56FVSYY86 pKa = 9.52MSPLAGHH93 pKa = 6.13VGCVFFVVIPAKK105 pKa = 10.37GLNSRR110 pKa = 11.84ISADD114 pKa = 3.46EE115 pKa = 4.39AEE117 pKa = 4.67SLQSAILWDD126 pKa = 3.52EE127 pKa = 4.0KK128 pKa = 11.12GRR130 pKa = 11.84LKK132 pKa = 9.41ITPISGPISRR142 pKa = 11.84HH143 pKa = 4.37PWTNLSQVVTPPQIPKK159 pKa = 10.5GSTDD163 pKa = 3.66GEE165 pKa = 4.24RR166 pKa = 11.84QDD168 pKa = 4.35LQSGYY173 pKa = 10.67YY174 pKa = 10.53LIFDD178 pKa = 3.63SRR180 pKa = 11.84KK181 pKa = 10.0LFGKK185 pKa = 10.27DD186 pKa = 3.26LVDD189 pKa = 3.54KK190 pKa = 11.1QSVLGEE196 pKa = 4.05LSLTITATYY205 pKa = 6.88WTSLSS210 pKa = 3.57

Molecular weight: 23.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1358

210

860

452.7

50.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.627 ± 0.315

2.062 ± 0.457

5.523 ± 0.323

6.112 ± 0.667

3.24 ± 0.772

6.922 ± 0.42

1.841 ± 0.325

4.86 ± 1.115

5.081 ± 0.753

10.309 ± 0.455

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.694 ± 0.201

3.387 ± 0.498

6.701 ± 0.688

3.166 ± 0.573

8.91 ± 0.966

7.585 ± 1.055

4.934 ± 1.024

6.186 ± 0.486

1.62 ± 0.24

3.24 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski