Plasmodium falciparum (isolate Palo Alto / Uganda)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida; Plasmodiidae; Plasmodium; Plasmodium (Laverania); Plasmodium falciparum

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6037 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4J5V7|W4J5V7_PLAFP Uncharacterized protein (Fragment) OS=Plasmodium falciparum (isolate Palo Alto / Uganda) OX=57270 GN=PFUGPA_00996 PE=4 SV=1
MM1 pKa = 8.65LEE3 pKa = 3.94EE4 pKa = 4.27RR5 pKa = 11.84NEE7 pKa = 3.97NNEE10 pKa = 3.93NNEE13 pKa = 4.01DD14 pKa = 3.92NEE16 pKa = 4.49NNGYY20 pKa = 8.4NQNNEE25 pKa = 3.51YY26 pKa = 10.56DD27 pKa = 3.53QNNEE31 pKa = 3.35YY32 pKa = 10.64DD33 pKa = 3.51QNNEE37 pKa = 3.35YY38 pKa = 10.64DD39 pKa = 3.51QNNEE43 pKa = 3.35YY44 pKa = 10.64DD45 pKa = 3.51QNNEE49 pKa = 3.35YY50 pKa = 10.64DD51 pKa = 3.51QNNEE55 pKa = 3.35YY56 pKa = 10.64DD57 pKa = 3.51QNNEE61 pKa = 3.37YY62 pKa = 10.72DD63 pKa = 3.43QNNDD67 pKa = 3.01YY68 pKa = 10.88NQNNDD73 pKa = 3.17YY74 pKa = 10.78NQNNDD79 pKa = 3.17YY80 pKa = 10.78NQNNDD85 pKa = 3.17YY86 pKa = 10.78NQNNDD91 pKa = 3.17YY92 pKa = 10.78NQNNDD97 pKa = 3.06YY98 pKa = 10.96NLNSGYY104 pKa = 10.55NLNSGYY110 pKa = 10.59NLNSGYY116 pKa = 10.36NLNSGYY122 pKa = 10.45SLNSGYY128 pKa = 10.98SLNNVYY134 pKa = 10.32NQYY137 pKa = 11.36SNNIEE142 pKa = 3.87NSGNILNNGFFGSSTNNNNRR162 pKa = 11.84SILGGVRR169 pKa = 11.84EE170 pKa = 4.58PIQLEE175 pKa = 4.0SRR177 pKa = 11.84GFFINGRR184 pKa = 11.84SNNVNRR190 pKa = 11.84ILVNVDD196 pKa = 3.25HH197 pKa = 7.1NNVPINSLNGTNHH210 pKa = 6.96ILGIPNNINMDD221 pKa = 4.0SLIQTILLASSLIINTSARR240 pKa = 11.84AVATSNRR247 pKa = 11.84NTRR250 pKa = 11.84NSNALTSDD258 pKa = 3.54GEE260 pKa = 4.28EE261 pKa = 4.05TMEE264 pKa = 4.89EE265 pKa = 4.03IVDD268 pKa = 4.02DD269 pKa = 4.22GMEE272 pKa = 4.32EE273 pKa = 3.7IDD275 pKa = 4.67LNEE278 pKa = 4.22NSDD281 pKa = 4.44FIHH284 pKa = 7.4DD285 pKa = 4.13GNPLGEE291 pKa = 4.28YY292 pKa = 10.96SNMEE296 pKa = 4.0YY297 pKa = 10.9EE298 pKa = 4.48LLNEE302 pKa = 4.48VSASTDD308 pKa = 3.38FSDD311 pKa = 3.5YY312 pKa = 11.01HH313 pKa = 7.75SVVDD317 pKa = 3.89EE318 pKa = 4.68DD319 pKa = 5.63LINFNDD325 pKa = 3.99DD326 pKa = 3.11VTLEE330 pKa = 4.2TQSMIAHH337 pKa = 6.92GGSLSEE343 pKa = 4.27IEE345 pKa = 4.17EE346 pKa = 4.39TGDD349 pKa = 3.66LSSDD353 pKa = 3.24VDD355 pKa = 3.74RR356 pKa = 11.84LLSSIEE362 pKa = 3.84TTPRR366 pKa = 11.84DD367 pKa = 3.48DD368 pKa = 3.26VVYY371 pKa = 10.56IRR373 pKa = 11.84GDD375 pKa = 3.33IILGEE380 pKa = 4.14DD381 pKa = 3.84EE382 pKa = 5.19IIIEE386 pKa = 4.18NGEE389 pKa = 4.12IILNNTDD396 pKa = 3.64EE397 pKa = 4.7LFEE400 pKa = 5.13DD401 pKa = 4.03VPEE404 pKa = 4.08MLEE407 pKa = 4.01NANLLEE413 pKa = 4.49DD414 pKa = 3.8RR415 pKa = 11.84EE416 pKa = 4.66HH417 pKa = 7.44VFEE420 pKa = 4.42YY421 pKa = 10.54NISVANEE428 pKa = 3.48NTTNQNSYY436 pKa = 10.71NFNAPQDD443 pKa = 4.02NTLTNYY449 pKa = 10.32NISRR453 pKa = 11.84FLQRR457 pKa = 11.84YY458 pKa = 6.69GTTTPNTQHH467 pKa = 6.75NISIHH472 pKa = 6.53RR473 pKa = 11.84DD474 pKa = 3.18GHH476 pKa = 5.2SLRR479 pKa = 11.84SDD481 pKa = 3.19TNIYY485 pKa = 10.86DD486 pKa = 4.61RR487 pKa = 11.84INGPSIRR494 pKa = 11.84EE495 pKa = 3.67NSLYY499 pKa = 10.28PGSFEE504 pKa = 3.77MAQYY508 pKa = 11.03ADD510 pKa = 4.72RR511 pKa = 11.84YY512 pKa = 10.15QMGHH516 pKa = 6.64TIAEE520 pKa = 4.43GFLYY524 pKa = 10.73

Molecular weight: 59.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4J717|W4J717_PLAFP GpcrRhopsn4 domain-containing protein OS=Plasmodium falciparum (isolate Palo Alto / Uganda) OX=57270 GN=PFUGPA_00578 PE=4 SV=1
MM1 pKa = 7.67LGPRR5 pKa = 11.84LLQSRR10 pKa = 11.84PGLVKK15 pKa = 10.22VRR17 pKa = 11.84RR18 pKa = 11.84TLVMLGPRR26 pKa = 11.84LLQSRR31 pKa = 11.84PKK33 pKa = 10.67LVTQRR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 10.41LVMLGPIFVTLRR52 pKa = 11.84PKK54 pKa = 10.33LVNLGPIFVKK64 pKa = 10.58LRR66 pKa = 11.84PQLVNVRR73 pKa = 11.84PKK75 pKa = 10.36LVKK78 pKa = 10.54FGPMLVNVRR87 pKa = 11.84PMLVEE92 pKa = 3.87VV93 pKa = 4.11

Molecular weight: 10.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5988

49

6037

3952059

11

10294

654.6

77.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.939 ± 0.026

1.787 ± 0.018

6.358 ± 0.029

6.967 ± 0.048

4.436 ± 0.029

2.793 ± 0.027

2.429 ± 0.014

9.352 ± 0.041

11.744 ± 0.049

7.642 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.217 ± 0.013

14.459 ± 0.104

1.958 ± 0.022

2.751 ± 0.018

2.644 ± 0.018

6.394 ± 0.025

4.094 ± 0.022

3.757 ± 0.021

0.497 ± 0.01

5.782 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski