Trichormus variabilis SAG 1403-4b

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Nostocales; Nostocaceae; Trichormus; Trichormus variabilis

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5168 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3S5K2M9|A0A3S5K2M9_ANAVA DUF262 domain-containing protein OS=Trichormus variabilis SAG 1403-4b OX=447716 GN=DSM107003_49640 PE=4 SV=1
MM1 pKa = 7.51SRR3 pKa = 11.84PQVKK7 pKa = 8.45ITVSASGGPLNTPSYY22 pKa = 11.08KK23 pKa = 10.6DD24 pKa = 3.35LTITGIGTDD33 pKa = 3.27NTYY36 pKa = 9.32TFLNQQGVLDD46 pKa = 4.29AWCLDD51 pKa = 3.38SATGISSNTTYY62 pKa = 9.86TANVYY67 pKa = 10.52SSYY70 pKa = 11.25EE71 pKa = 3.96LGALATLGTPARR83 pKa = 11.84IGNLYY88 pKa = 10.75NLDD91 pKa = 3.66LVNWIINQNFAANPQYY107 pKa = 11.31NYY109 pKa = 11.33GEE111 pKa = 4.0IQAAIWTLLGDD122 pKa = 4.53TYY124 pKa = 11.37NSSGADD130 pKa = 3.34LTRR133 pKa = 11.84NGAVVASDD141 pKa = 3.64VNSIIALAKK150 pKa = 10.31ANGEE154 pKa = 4.04NFVPDD159 pKa = 3.71TNQFIGVIFDD169 pKa = 3.81PFVVNGDD176 pKa = 3.55GSVTPRR182 pKa = 11.84QPLIAQIKK190 pKa = 8.18AAKK193 pKa = 10.34LGDD196 pKa = 4.28LVWNDD201 pKa = 3.39LNANGIQDD209 pKa = 3.65TGEE212 pKa = 3.92SGIAGVTVNLTRR224 pKa = 11.84DD225 pKa = 3.52SNSNGIIEE233 pKa = 4.47SNEE236 pKa = 3.42ILATTTTNVSGLYY249 pKa = 9.12EE250 pKa = 4.26FRR252 pKa = 11.84GLTPGLSYY260 pKa = 10.35QVQFATPTGFDD271 pKa = 3.13AVSPRR276 pKa = 11.84QQGGNQTTDD285 pKa = 2.4SDD287 pKa = 4.27GLVSNVVILNPGEE300 pKa = 3.98FNQTIDD306 pKa = 3.17AGFYY310 pKa = 10.2KK311 pKa = 10.51YY312 pKa = 11.03ASLGDD317 pKa = 3.59FVFNDD322 pKa = 3.68GNNNGIQDD330 pKa = 3.76AGEE333 pKa = 4.05AGIGGVTVEE342 pKa = 5.58LINPANGSVIATTTTDD358 pKa = 3.82GIGAYY363 pKa = 8.44TFTGLTPGDD372 pKa = 3.82YY373 pKa = 9.53QVKK376 pKa = 8.04FTSPTGYY383 pKa = 10.33VFSTANVGDD392 pKa = 3.85DD393 pKa = 4.3TKK395 pKa = 11.6DD396 pKa = 3.11SDD398 pKa = 4.67ANTSTGLTKK407 pKa = 10.23TVTLTSGEE415 pKa = 4.31FNGTLDD421 pKa = 3.63AGLVQLASLGDD432 pKa = 3.93KK433 pKa = 10.5VFHH436 pKa = 7.25DD437 pKa = 5.01LDD439 pKa = 5.64ADD441 pKa = 5.05GIQDD445 pKa = 3.86AGEE448 pKa = 3.86QGIANASVKK457 pKa = 10.77LLDD460 pKa = 3.56TAGNVIATTTTDD472 pKa = 3.08GDD474 pKa = 3.85GLYY477 pKa = 10.64SFTNLQPGDD486 pKa = 3.73YY487 pKa = 9.59KK488 pKa = 11.37VQFVQPTGFNGVSLANVGGDD508 pKa = 4.01DD509 pKa = 4.78SVDD512 pKa = 3.57SDD514 pKa = 4.01GLISDD519 pKa = 4.49VVNLSPGEE527 pKa = 3.94NDD529 pKa = 3.38TTVDD533 pKa = 2.85AGFYY537 pKa = 8.57KK538 pKa = 10.21TASLGDD544 pKa = 3.5FVFNDD549 pKa = 3.68GNNNGIQDD557 pKa = 3.76AGEE560 pKa = 4.06AGIGEE565 pKa = 4.41VTVEE569 pKa = 5.02LINPADD575 pKa = 3.81GSVIATTTTDD585 pKa = 2.84EE586 pKa = 4.13TGAYY590 pKa = 7.47TFTGLTPGDD599 pKa = 3.82YY600 pKa = 9.53QVKK603 pKa = 8.25FTSPTDD609 pKa = 3.87YY610 pKa = 11.14VFSTADD616 pKa = 2.99QGGDD620 pKa = 3.44DD621 pKa = 4.5TVDD624 pKa = 3.2SDD626 pKa = 5.04ANTSTGLTQTVTLTSGEE643 pKa = 4.31FNGTLDD649 pKa = 3.63AGLVQLASLGDD660 pKa = 3.84FVFEE664 pKa = 4.86DD665 pKa = 3.94SNANGIQDD673 pKa = 3.09AWEE676 pKa = 3.81QGIAEE681 pKa = 4.27ASVNLLDD688 pKa = 3.78TAGNVIATTTTDD700 pKa = 3.08GDD702 pKa = 3.85GLYY705 pKa = 10.64SFTNLQPGDD714 pKa = 3.73YY715 pKa = 9.59KK716 pKa = 11.41VQFVQPAGFSGVSPSNVGGDD736 pKa = 3.97DD737 pKa = 4.02SVDD740 pKa = 3.57SDD742 pKa = 4.01GLISDD747 pKa = 4.49VVNLSPGEE755 pKa = 3.94NDD757 pKa = 3.38TTVDD761 pKa = 2.85AGFYY765 pKa = 8.57KK766 pKa = 10.21TASLGDD772 pKa = 3.5FVFNDD777 pKa = 3.68GNNNGIQDD785 pKa = 3.45IGEE788 pKa = 4.16AGIGGVTVEE797 pKa = 5.58LINPANGSVIASVITDD813 pKa = 3.89DD814 pKa = 3.48NGGYY818 pKa = 9.52QFSGLTPGDD827 pKa = 3.7YY828 pKa = 9.61QVKK831 pKa = 8.04FTSPTGYY838 pKa = 10.34VFSTAYY844 pKa = 10.15QGGDD848 pKa = 3.36DD849 pKa = 4.57TVDD852 pKa = 3.2SDD854 pKa = 5.04ANTSTGLTKK863 pKa = 10.23TVTLTSGEE871 pKa = 4.31FNGTLDD877 pKa = 3.63AGLVQLASLGDD888 pKa = 3.93KK889 pKa = 10.5VFHH892 pKa = 7.25DD893 pKa = 5.01LDD895 pKa = 5.64ADD897 pKa = 5.05GIQDD901 pKa = 3.86AGEE904 pKa = 3.86QGIANASVKK913 pKa = 10.77LLDD916 pKa = 3.56TAGNVIATTTTDD928 pKa = 3.08GDD930 pKa = 3.88GLYY933 pKa = 10.82SFINLQPGDD942 pKa = 3.83YY943 pKa = 9.82KK944 pKa = 11.37VQFVQPTDD952 pKa = 3.8FNGVSLANVGGDD964 pKa = 4.01DD965 pKa = 4.78SVDD968 pKa = 3.57SDD970 pKa = 4.01GLISDD975 pKa = 4.49VVNLSPGEE983 pKa = 3.94NDD985 pKa = 3.38TTVDD989 pKa = 2.85AGFYY993 pKa = 8.57KK994 pKa = 10.21TASLGDD1000 pKa = 3.5FVFNDD1005 pKa = 3.68GNNNGIQDD1013 pKa = 3.76AGEE1016 pKa = 4.05AGIGGVTVKK1025 pKa = 10.8LINPANGNVISTTTTDD1041 pKa = 3.03GAGAYY1046 pKa = 7.07TFTGLTPGDD1055 pKa = 3.84YY1056 pKa = 9.35QVKK1059 pKa = 9.7FISPTGYY1066 pKa = 10.67SFTAANQGGNDD1077 pKa = 4.28AVDD1080 pKa = 3.56SDD1082 pKa = 4.92ANPSTGLTQTVTLTSGEE1099 pKa = 4.32YY1100 pKa = 10.52NGTLDD1105 pKa = 4.27AGLVQLASLGDD1116 pKa = 3.93KK1117 pKa = 10.5VFHH1120 pKa = 7.25DD1121 pKa = 5.01LDD1123 pKa = 5.64ADD1125 pKa = 5.05GIQDD1129 pKa = 3.86AGEE1132 pKa = 3.86QGIANASVKK1141 pKa = 10.77LLDD1144 pKa = 3.56TAGNVIATTTTDD1156 pKa = 3.08GDD1158 pKa = 3.85GLYY1161 pKa = 10.64SFTNLQPGDD1170 pKa = 3.73YY1171 pKa = 9.59KK1172 pKa = 11.37VQFVQPTGFNGVSLANVGGDD1192 pKa = 3.78DD1193 pKa = 5.29SVDD1196 pKa = 3.18SDD1198 pKa = 3.89AASNLTTGVVNLSPGEE1214 pKa = 3.94NDD1216 pKa = 3.38TTVDD1220 pKa = 2.85AGFYY1224 pKa = 8.64KK1225 pKa = 10.25TASLGNFVFNDD1236 pKa = 3.34VNNNGIQEE1244 pKa = 3.98ASEE1247 pKa = 4.27AGVAGVTVTLTGGGADD1263 pKa = 3.84GLISTTADD1271 pKa = 2.81NTTITTTTNASGKK1284 pKa = 10.27YY1285 pKa = 9.23NFAGLTPGQEE1295 pKa = 4.26YY1296 pKa = 10.07QVGFSNLPAGYY1307 pKa = 10.36QFTQANAGTDD1317 pKa = 3.53DD1318 pKa = 5.35ALDD1321 pKa = 3.86SDD1323 pKa = 5.42ANTSTGKK1330 pKa = 7.77TQIVTLASGEE1340 pKa = 4.29NNTTLDD1346 pKa = 3.17AGIYY1350 pKa = 9.65QNAGDD1355 pKa = 4.69LSITKK1360 pKa = 9.53TNGLTTVSPGQQITYY1375 pKa = 9.2TIVAQNNGLITATNALVSDD1394 pKa = 5.22IIPSNLTNVTWTSVATGGATDD1415 pKa = 3.9NQSSGTGSINDD1426 pKa = 4.01YY1427 pKa = 9.5VTLTAGSSITYY1438 pKa = 8.23TVTGTVAPNALTPVYY1453 pKa = 8.18NTPTTINLTSNSSSALNGTAGNIRR1477 pKa = 11.84NFSANGISVNASAFSSTSTGTWNTAYY1503 pKa = 10.68LGLYY1507 pKa = 9.38GSYY1510 pKa = 11.06GLGVTDD1516 pKa = 4.27GGEE1519 pKa = 3.89NGSNGTHH1526 pKa = 7.02HH1527 pKa = 7.36IDD1529 pKa = 3.12NDD1531 pKa = 3.32GRR1533 pKa = 11.84NNYY1536 pKa = 9.11VLLEE1540 pKa = 4.14FSDD1543 pKa = 3.85SVVIDD1548 pKa = 3.95KK1549 pKa = 10.9AALSYY1554 pKa = 11.27VVGDD1558 pKa = 3.5SDD1560 pKa = 3.62ISVWIGNFNNPYY1572 pKa = 9.47TNHH1575 pKa = 6.17LTLNSSVLSSFGFTEE1590 pKa = 3.95INLGGSSDD1598 pKa = 3.79RR1599 pKa = 11.84NADD1602 pKa = 3.12INASNIVGNAVVIAAKK1618 pKa = 10.73VGDD1621 pKa = 3.98TNDD1624 pKa = 3.43EE1625 pKa = 4.37FKK1627 pKa = 11.13LKK1629 pKa = 10.81SLDD1632 pKa = 3.5IIKK1635 pKa = 9.23ATTQTGGSLVNTATITAPTGFTDD1658 pKa = 3.58TNSNNNSATDD1668 pKa = 3.23TDD1670 pKa = 4.38TIVASSTVRR1679 pKa = 11.84IGDD1682 pKa = 3.74RR1683 pKa = 11.84VWYY1686 pKa = 7.32DD1687 pKa = 3.32TNANGIQEE1695 pKa = 4.16TGEE1698 pKa = 4.0AGVAGVAVNLFNQAGTQVGTTTTDD1722 pKa = 2.83TNGFYY1727 pKa = 10.54QFTANTNSTYY1737 pKa = 10.71SVQFVQPTGFSGFTTANVGNDD1758 pKa = 3.61GADD1761 pKa = 3.24SDD1763 pKa = 4.55VVNANGTTAQFLVGTTDD1780 pKa = 3.26NLTIDD1785 pKa = 3.64AGLIKK1790 pKa = 10.76NIGDD1794 pKa = 3.81LSITKK1799 pKa = 9.48TDD1801 pKa = 3.16GLTTVTAGQQLTYY1814 pKa = 10.31TIVVSNNGFITATNALVTDD1833 pKa = 5.92AIPALLTNVTWTSVASGGATGNEE1856 pKa = 3.93TSGTGNINDD1865 pKa = 3.9YY1866 pKa = 9.57VTLTGGSSITYY1877 pKa = 7.49TVNGTVAANAVSSSSTYY1894 pKa = 9.84TDD1896 pKa = 2.52TRR1898 pKa = 11.84FDD1900 pKa = 4.14FNGNSSSDD1908 pKa = 3.44GTDD1911 pKa = 2.82GNTRR1915 pKa = 11.84TFTKK1919 pKa = 10.61DD1920 pKa = 2.88GITVTARR1927 pKa = 11.84AFSRR1931 pKa = 11.84VDD1933 pKa = 3.26GTNGAWSKK1941 pKa = 11.29AYY1943 pKa = 10.28LGSYY1947 pKa = 9.13TGGLGVTDD1955 pKa = 4.03SSEE1958 pKa = 3.83SSSYY1962 pKa = 10.79HH1963 pKa = 6.01RR1964 pKa = 11.84VDD1966 pKa = 3.43NGGGRR1971 pKa = 11.84DD1972 pKa = 3.58NYY1974 pKa = 10.9VLFQFSEE1981 pKa = 4.37AVVVDD1986 pKa = 3.89SAYY1989 pKa = 10.66LQYY1992 pKa = 11.4VVTDD1996 pKa = 3.36SDD1998 pKa = 3.82TSVWIGNFNNTITTLSDD2015 pKa = 3.22SVLNSFGFTEE2025 pKa = 4.4VNLGSGSDD2033 pKa = 3.11RR2034 pKa = 11.84WADD2037 pKa = 3.27INAGNYY2043 pKa = 9.46LGNTLVIAAKK2053 pKa = 9.9TDD2055 pKa = 3.43DD2056 pKa = 3.81TNDD2059 pKa = 3.23NFKK2062 pKa = 10.32IRR2064 pKa = 11.84HH2065 pKa = 6.24LDD2067 pKa = 3.04IQKK2070 pKa = 9.94PVAPTTVSLTNTATVTAPTGFTDD2093 pKa = 3.71TNTSNNSATDD2103 pKa = 3.44TDD2105 pKa = 4.48TVTAAPTVPSAPGVRR2120 pKa = 11.84TPGFWQNTEE2129 pKa = 3.64WQKK2132 pKa = 10.89FWDD2135 pKa = 5.33GIQGNEE2141 pKa = 4.28PAQKK2145 pKa = 8.27TQPNFAKK2152 pKa = 10.33SDD2154 pKa = 3.87LLFAPYY2160 pKa = 9.58TNSAQAGKK2168 pKa = 10.59VLDD2171 pKa = 4.33PVSGQYY2177 pKa = 10.67DD2178 pKa = 3.32IGLLIGDD2185 pKa = 4.45FNRR2188 pKa = 11.84NGKK2191 pKa = 7.69TDD2193 pKa = 3.35AGEE2196 pKa = 4.01DD2197 pKa = 3.46TLFYY2201 pKa = 10.77TRR2203 pKa = 11.84AQALQMVDD2211 pKa = 4.39ASAHH2215 pKa = 5.63PNGDD2219 pKa = 2.69KK2220 pKa = 10.61RR2221 pKa = 11.84YY2222 pKa = 9.23DD2223 pKa = 3.4LGRR2226 pKa = 11.84SLVAGWLNYY2235 pKa = 10.25LAGNPIDD2242 pKa = 3.98TANPTDD2248 pKa = 3.68KK2249 pKa = 11.14DD2250 pKa = 3.02EE2251 pKa = 4.64RR2252 pKa = 11.84YY2253 pKa = 10.42YY2254 pKa = 10.51INEE2257 pKa = 4.76GINWLQGFTPDD2268 pKa = 4.17EE2269 pKa = 4.51NGDD2272 pKa = 3.75KK2273 pKa = 10.95KK2274 pKa = 11.25GDD2276 pKa = 3.45GAIYY2280 pKa = 10.45QMTGSSVSSPQLTNSYY2296 pKa = 9.01WNLGISSASNLPSSYY2311 pKa = 10.82KK2312 pKa = 10.79SNTNVLYY2319 pKa = 9.51PLEE2322 pKa = 4.67SGSVINTNLDD2332 pKa = 3.28NYY2334 pKa = 11.31NNGLGLADD2342 pKa = 3.36NVFYY2346 pKa = 11.01GGNAA2350 pKa = 3.15

Molecular weight:
242.94 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A433UWE5|A0A433UWE5_ANAVA Uncharacterized protein OS=Trichormus variabilis SAG 1403-4b OX=447716 GN=DSM107003_12610 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.03PNYY5 pKa = 9.07RR6 pKa = 11.84RR7 pKa = 11.84CISCRR12 pKa = 11.84RR13 pKa = 11.84VGLKK17 pKa = 10.37EE18 pKa = 3.9EE19 pKa = 4.36FWRR22 pKa = 11.84IVRR25 pKa = 11.84VFPSGKK31 pKa = 9.1VQLDD35 pKa = 3.24QGMGRR40 pKa = 11.84SAYY43 pKa = 9.32ICPQTSCLQAAQKK56 pKa = 10.5KK57 pKa = 8.83NRR59 pKa = 11.84LGRR62 pKa = 11.84SLHH65 pKa = 5.08GTVPEE70 pKa = 4.21TLYY73 pKa = 9.23QTLWLRR79 pKa = 11.84LTQNNTQNQII89 pKa = 3.23

Molecular weight:
10.37 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5168

0

5168

1610029

41

3312

311.5

34.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.704 ± 0.033

0.971 ± 0.012

4.838 ± 0.027

6.374 ± 0.034

3.972 ± 0.023

6.499 ± 0.039

1.805 ± 0.016

7.239 ± 0.03

5.234 ± 0.034

10.926 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.834 ± 0.016

4.772 ± 0.036

4.565 ± 0.027

5.388 ± 0.034

4.73 ± 0.028

6.382 ± 0.03

5.753 ± 0.029

6.487 ± 0.028

1.408 ± 0.015

3.109 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski