Azoarcus sp. KH32C

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Rhodocyclales; Zoogloeaceae; Azoarcus; unclassified Azoarcus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5176 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H0Q3D0|H0Q3D0_9RHOO NAD(P)H dehydrogenase (quinone) OS=Azoarcus sp. KH32C OX=748247 GN=wrbA PE=3 SV=1
MM1 pKa = 7.0NTTAQNLAVLAAMLGLCVSSAYY23 pKa = 10.08AASAPDD29 pKa = 3.18QGGSARR35 pKa = 11.84ISGVTVEE42 pKa = 4.99SPLARR47 pKa = 11.84KK48 pKa = 9.05GADD51 pKa = 4.08DD52 pKa = 4.66PPPAPGCDD60 pKa = 3.24DD61 pKa = 4.67HH62 pKa = 7.07GTDD65 pKa = 4.07LCQSEE70 pKa = 4.37LAKK73 pKa = 11.03NGADD77 pKa = 4.46DD78 pKa = 4.98PMPQPGCDD86 pKa = 3.22DD87 pKa = 4.52HH88 pKa = 6.99GTDD91 pKa = 4.98LCLTPTAIEE100 pKa = 4.14TT101 pKa = 3.82

Molecular weight: 10.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H0Q037|H0Q037_9RHOO 2-isopropylmalate synthase OS=Azoarcus sp. KH32C OX=748247 GN=leuA PE=3 SV=1
MM1 pKa = 6.96NTTTVGVDD9 pKa = 3.13LAKK12 pKa = 10.79NVFVTCVADD21 pKa = 3.62GAGRR25 pKa = 11.84VIEE28 pKa = 3.98TRR30 pKa = 11.84EE31 pKa = 3.88FNRR34 pKa = 11.84SGFLAWLSTLPRR46 pKa = 11.84GTLVGMEE53 pKa = 4.2ACGGAHH59 pKa = 5.79CWGRR63 pKa = 11.84TMQSLGLEE71 pKa = 4.28PRR73 pKa = 11.84LMAPEE78 pKa = 4.1FVRR81 pKa = 11.84PYY83 pKa = 10.51RR84 pKa = 11.84KK85 pKa = 9.6RR86 pKa = 11.84QAVKK90 pKa = 10.39NDD92 pKa = 3.28RR93 pKa = 11.84ADD95 pKa = 3.18AVAIVAALLAPGMRR109 pKa = 11.84FIPVKK114 pKa = 10.29TEE116 pKa = 3.57AQQQRR121 pKa = 11.84LAWHH125 pKa = 6.11SLRR128 pKa = 11.84LGWIEE133 pKa = 4.64EE134 pKa = 4.06RR135 pKa = 11.84TALLNRR141 pKa = 11.84IRR143 pKa = 11.84GLLAEE148 pKa = 4.99FGMVVDD154 pKa = 4.5TGAMRR159 pKa = 11.84LRR161 pKa = 11.84RR162 pKa = 11.84KK163 pKa = 9.46LAEE166 pKa = 4.31HH167 pKa = 6.67EE168 pKa = 4.27FDD170 pKa = 3.6TAQPAPIRR178 pKa = 11.84QLIQGVRR185 pKa = 11.84DD186 pKa = 3.45QLDD189 pKa = 3.44ALDD192 pKa = 4.83ARR194 pKa = 11.84IAEE197 pKa = 4.77CDD199 pKa = 3.41RR200 pKa = 11.84QIATQQADD208 pKa = 3.31DD209 pKa = 3.5AAARR213 pKa = 11.84RR214 pKa = 11.84VRR216 pKa = 11.84DD217 pKa = 3.58LPGVGLLTADD227 pKa = 3.45AVVASVGDD235 pKa = 3.23ATMFHH240 pKa = 7.03NGRR243 pKa = 11.84QFAAWLGLTPSQYY256 pKa = 11.14SSGGKK261 pKa = 8.98PKK263 pKa = 10.47LGRR266 pKa = 11.84ITRR269 pKa = 11.84RR270 pKa = 11.84GDD272 pKa = 3.07DD273 pKa = 3.56YY274 pKa = 11.76LRR276 pKa = 11.84TLLVQGARR284 pKa = 11.84SVLAAALRR292 pKa = 11.84KK293 pKa = 9.52ARR295 pKa = 11.84NTRR298 pKa = 11.84EE299 pKa = 3.7ALTRR303 pKa = 11.84LQQWIVTLHH312 pKa = 5.97ARR314 pKa = 11.84VGYY317 pKa = 10.27HH318 pKa = 5.32KK319 pKa = 9.54TLVAIANKK327 pKa = 9.69HH328 pKa = 5.55ARR330 pKa = 11.84QLWAVLAKK338 pKa = 10.81GEE340 pKa = 4.48TYY342 pKa = 10.77NPEE345 pKa = 3.75AWRR348 pKa = 11.84QRR350 pKa = 11.84PDD352 pKa = 3.08SATEE356 pKa = 3.66PARR359 pKa = 3.8

Molecular weight: 39.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5176

0

5176

1764397

41

2915

340.9

37.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.335 ± 0.045

1.027 ± 0.012

5.57 ± 0.027

5.979 ± 0.03

3.644 ± 0.022

8.367 ± 0.036

2.255 ± 0.016

4.757 ± 0.024

3.124 ± 0.027

10.537 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.015

2.638 ± 0.018

5.059 ± 0.024

3.325 ± 0.021

7.415 ± 0.038

5.252 ± 0.023

5.025 ± 0.033

7.675 ± 0.03

1.351 ± 0.013

2.279 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski