Beihai weivirus-like virus 4

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1L3KLC2|A0A1L3KLC2_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 4 OX=1922752 PE=4 SV=1
MM1 pKa = 7.44LALRR5 pKa = 11.84LGQTDD10 pKa = 3.69RR11 pKa = 11.84QIGEE15 pKa = 4.02VAPFLLRR22 pKa = 11.84HH23 pKa = 4.98AAGRR27 pKa = 11.84QGHH30 pKa = 5.21WRR32 pKa = 11.84ITAQGYY38 pKa = 7.54DD39 pKa = 3.37HH40 pKa = 7.68PAAQSTKK47 pKa = 9.57AYY49 pKa = 9.27SVVYY53 pKa = 9.96TEE55 pKa = 5.04EE56 pKa = 4.21EE57 pKa = 3.95FSKK60 pKa = 10.69LSRR63 pKa = 11.84VVYY66 pKa = 10.29HH67 pKa = 6.5GLRR70 pKa = 11.84NSTKK74 pKa = 8.63PTSLHH79 pKa = 6.52ASLEE83 pKa = 4.22NVAQVLWPTGSPDD96 pKa = 3.32HH97 pKa = 6.3VAMRR101 pKa = 11.84HH102 pKa = 4.55VGVFVMMQYY111 pKa = 7.27MTSDD115 pKa = 3.33EE116 pKa = 4.38TAVRR120 pKa = 11.84PLARR124 pKa = 11.84WAMVAGVGIGLMVRR138 pKa = 11.84SVADD142 pKa = 3.52GWKK145 pKa = 9.77LATIAGLSPVLLARR159 pKa = 11.84LVSRR163 pKa = 11.84GRR165 pKa = 11.84AIPILEE171 pKa = 3.98RR172 pKa = 11.84VGASQAAVAPNPGGSGPPPQAAPAPPPPPQGEE204 pKa = 4.18PPPPGDD210 pKa = 3.62AEE212 pKa = 4.05TDD214 pKa = 3.02EE215 pKa = 4.46GVRR218 pKa = 11.84VEE220 pKa = 4.66EE221 pKa = 4.54LNEE224 pKa = 4.03EE225 pKa = 4.31PPVIMDD231 pKa = 4.25TPALPDD237 pKa = 3.93DD238 pKa = 4.54AATAAAASNDD248 pKa = 3.27RR249 pKa = 11.84RR250 pKa = 11.84TMQVGDD256 pKa = 3.72VVAVIGQAFNKK267 pKa = 10.1DD268 pKa = 3.24EE269 pKa = 4.81PVNHH273 pKa = 6.04MPVVGCLVGPCQVKK287 pKa = 9.9PNVYY291 pKa = 10.71AKK293 pKa = 9.47TASNLKK299 pKa = 10.03AAIEE303 pKa = 3.82EE304 pKa = 4.64RR305 pKa = 11.84ITKK308 pKa = 10.09KK309 pKa = 10.42ARR311 pKa = 11.84KK312 pKa = 8.42CQLSKK317 pKa = 10.63KK318 pKa = 9.74DD319 pKa = 3.26KK320 pKa = 10.75ARR322 pKa = 11.84IGGLVRR328 pKa = 11.84QSMSCHH334 pKa = 5.6RR335 pKa = 11.84VRR337 pKa = 11.84GVFSKK342 pKa = 10.87RR343 pKa = 11.84KK344 pKa = 7.73IEE346 pKa = 3.6AWAIEE351 pKa = 4.03NLDD354 pKa = 3.86LEE356 pKa = 5.28LIRR359 pKa = 11.84SGKK362 pKa = 8.94WSVEE366 pKa = 3.67RR367 pKa = 11.84FRR369 pKa = 11.84ASLEE373 pKa = 3.58ALYY376 pKa = 10.81ARR378 pKa = 11.84EE379 pKa = 3.81YY380 pKa = 9.16PTYY383 pKa = 10.29QFKK386 pKa = 11.08AGIKK390 pKa = 7.88PEE392 pKa = 4.04CMAEE396 pKa = 4.19GKK398 pKa = 10.24APRR401 pKa = 11.84MLIADD406 pKa = 4.1GDD408 pKa = 4.23DD409 pKa = 3.83GQLMALAVVRR419 pKa = 11.84CFEE422 pKa = 4.66DD423 pKa = 3.6LLFEE427 pKa = 4.22HH428 pKa = 7.01FEE430 pKa = 4.23RR431 pKa = 11.84KK432 pKa = 9.43SIKK435 pKa = 10.43HH436 pKa = 4.13MAKK439 pKa = 9.73RR440 pKa = 11.84EE441 pKa = 3.97AMDD444 pKa = 3.49RR445 pKa = 11.84VVKK448 pKa = 10.26EE449 pKa = 4.07LSKK452 pKa = 10.95KK453 pKa = 8.46GAKK456 pKa = 9.6AVEE459 pKa = 4.46GDD461 pKa = 3.29GSAWDD466 pKa = 3.82TTCNVEE472 pKa = 4.35IRR474 pKa = 11.84ALVEE478 pKa = 4.03NPVLRR483 pKa = 11.84HH484 pKa = 5.07ICEE487 pKa = 4.22VLCGLGVVPEE497 pKa = 4.05SWLRR501 pKa = 11.84EE502 pKa = 3.66HH503 pKa = 7.25SAACEE508 pKa = 3.96QKK510 pKa = 9.31TLRR513 pKa = 11.84LFFKK517 pKa = 11.03NKK519 pKa = 8.0MEE521 pKa = 4.37SMSVTIDD528 pKa = 3.9AIRR531 pKa = 11.84RR532 pKa = 11.84SGHH535 pKa = 6.55RR536 pKa = 11.84GTSCLNWWVNFTLWVSSIFAEE557 pKa = 4.39PEE559 pKa = 3.74RR560 pKa = 11.84FLDD563 pKa = 3.56PDD565 pKa = 3.28VRR567 pKa = 11.84TGEE570 pKa = 4.5DD571 pKa = 3.0LTGQQRR577 pKa = 11.84WWNGCFEE584 pKa = 5.17GDD586 pKa = 5.31DD587 pKa = 4.28SLCTMKK593 pKa = 10.66PPMEE597 pKa = 4.64EE598 pKa = 3.82GDD600 pKa = 3.52RR601 pKa = 11.84MSEE604 pKa = 3.59IFLEE608 pKa = 4.92FWRR611 pKa = 11.84DD612 pKa = 2.68AGFNMKK618 pKa = 9.64IVFCTTRR625 pKa = 11.84ATFVGWHH632 pKa = 6.47IGCTDD637 pKa = 3.42GEE639 pKa = 4.63LNEE642 pKa = 4.39HH643 pKa = 7.02RR644 pKa = 11.84CPEE647 pKa = 4.16LPRR650 pKa = 11.84ALANSGVSVSPQGVEE665 pKa = 3.5AGRR668 pKa = 11.84DD669 pKa = 3.47GKK671 pKa = 11.25LSVVKK676 pKa = 10.36VLAAASALARR686 pKa = 11.84ASDD689 pKa = 3.83FSGILPSVSQKK700 pKa = 10.81YY701 pKa = 10.55LDD703 pKa = 3.81FAEE706 pKa = 4.75EE707 pKa = 4.46CTTSDD712 pKa = 3.72FEE714 pKa = 4.59DD715 pKa = 4.51RR716 pKa = 11.84EE717 pKa = 3.84MSYY720 pKa = 10.76RR721 pKa = 11.84AFGEE725 pKa = 4.07AGYY728 pKa = 9.93KK729 pKa = 10.6ANDD732 pKa = 3.15VRR734 pKa = 11.84EE735 pKa = 4.06QVQARR740 pKa = 11.84NLEE743 pKa = 4.07VTPEE747 pKa = 4.02KK748 pKa = 10.57EE749 pKa = 4.18QEE751 pKa = 3.95TLRR754 pKa = 11.84ALGYY758 pKa = 10.07QATHH762 pKa = 6.79DD763 pKa = 4.08EE764 pKa = 3.97IMTFRR769 pKa = 11.84EE770 pKa = 4.66YY771 pKa = 10.1IWSLEE776 pKa = 3.87PAALVAYY783 pKa = 10.22DD784 pKa = 3.97SFRR787 pKa = 11.84EE788 pKa = 4.01SLPPSWRR795 pKa = 11.84AAA797 pKa = 3.38

Molecular weight:
88.08 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1L3KLC2|A0A1L3KLC2_9VIRU Uncharacterized protein OS=Beihai weivirus-like virus 4 OX=1922752 PE=4 SV=1
MM1 pKa = 7.46TPNKK5 pKa = 9.1PAQRR9 pKa = 11.84NGRR12 pKa = 11.84KK13 pKa = 9.4LRR15 pKa = 11.84DD16 pKa = 3.47SGDD19 pKa = 3.16KK20 pKa = 10.46SKK22 pKa = 10.93RR23 pKa = 11.84PQRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84GGAASRR34 pKa = 11.84THH36 pKa = 6.65ANRR39 pKa = 11.84VLATGVGNVPTKK51 pKa = 10.57AWGSSRR57 pKa = 11.84GGSLLCWDD65 pKa = 4.81AKK67 pKa = 10.64HH68 pKa = 6.04PHH70 pKa = 6.55HH71 pKa = 7.04LSLPRR76 pKa = 11.84AVGPYY81 pKa = 5.61TTIRR85 pKa = 11.84ATRR88 pKa = 11.84RR89 pKa = 11.84VATGRR94 pKa = 11.84IANIIGTFKK103 pKa = 10.72SRR105 pKa = 11.84HH106 pKa = 5.19NSSSLGEE113 pKa = 4.06ASADD117 pKa = 3.98TKK119 pKa = 10.01WSEE122 pKa = 4.01IIMISDD128 pKa = 3.52HH129 pKa = 7.1VSANPINGTDD139 pKa = 3.02NTFFNNIDD147 pKa = 3.62LGEE150 pKa = 4.52LGSAATLVPSALTVQIVCPDD170 pKa = 3.2NLAADD175 pKa = 4.1GAKK178 pKa = 9.45GVVYY182 pKa = 10.35AGVMNTQAMVGGKK195 pKa = 9.42SDD197 pKa = 3.24SWDD200 pKa = 2.98AYY202 pKa = 9.0MNRR205 pKa = 11.84FVQFQSPRR213 pKa = 11.84MLAATKK219 pKa = 10.12LALRR223 pKa = 11.84GVTINSYY230 pKa = 9.59PLDD233 pKa = 3.65MSEE236 pKa = 5.15VSDD239 pKa = 3.56FTQLHH244 pKa = 5.22EE245 pKa = 4.62RR246 pKa = 11.84EE247 pKa = 4.17GLEE250 pKa = 3.96YY251 pKa = 9.92TGTMSSLSPQPAGWAPICIYY271 pKa = 10.56NPQGALLEE279 pKa = 4.02LLITVEE285 pKa = 3.69YY286 pKa = 10.09RR287 pKa = 11.84VRR289 pKa = 11.84FDD291 pKa = 4.25LEE293 pKa = 4.24HH294 pKa = 6.86PASASHH300 pKa = 5.16VHH302 pKa = 6.27HH303 pKa = 6.96PVASDD308 pKa = 3.62STWDD312 pKa = 3.27KK313 pKa = 10.14LTRR316 pKa = 11.84KK317 pKa = 9.76ASSLGHH323 pKa = 5.59GVMDD327 pKa = 4.11IADD330 pKa = 3.62VVANIGSAVRR340 pKa = 11.84TGRR343 pKa = 11.84QMLSAFGG350 pKa = 3.46

Molecular weight:
37.66 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1147

350

797

573.5

62.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.811 ± 0.133

1.744 ± 0.492

4.969 ± 0.096

6.452 ± 1.993

3.051 ± 0.425

7.672 ± 0.657

2.528 ± 0.499

3.836 ± 0.408

4.359 ± 0.358

7.759 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.964 ± 0.059

3.313 ± 0.856

5.754 ± 0.339

3.139 ± 0.156

7.323 ± 0.1

7.411 ± 1.277

5.231 ± 0.902

7.759 ± 0.5

2.005 ± 0.161

1.918 ± 0.045

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski