Amino acid dipepetide frequency for Enterobacteriaceae endosymbiont of Macroplea appendiculata

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.619AlaAla: 2.619 ± 0.193
0.589AlaCys: 0.589 ± 0.067
1.426AlaAsp: 1.426 ± 0.106
1.618AlaGlu: 1.618 ± 0.112
1.419AlaPhe: 1.419 ± 0.112
2.292AlaGly: 2.292 ± 0.125
1.121AlaHis: 1.121 ± 0.086
5.684AlaIle: 5.684 ± 0.209
3.399AlaLys: 3.399 ± 0.15
4.158AlaLeu: 4.158 ± 0.192
0.837AlaMet: 0.837 ± 0.085
2.157AlaAsn: 2.157 ± 0.146
0.901AlaPro: 0.901 ± 0.079
1.767AlaGln: 1.767 ± 0.103
1.59AlaArg: 1.59 ± 0.104
2.2AlaSer: 2.2 ± 0.157
2.108AlaThr: 2.108 ± 0.124
1.994AlaVal: 1.994 ± 0.125
0.298AlaTrp: 0.298 ± 0.053
1.845AlaTyr: 1.845 ± 0.13
0.0AlaXaa: 0.0 ± 0.0
Cys
0.624CysAla: 0.624 ± 0.065
0.227CysCys: 0.227 ± 0.046
0.532CysAsp: 0.532 ± 0.059
0.475CysGlu: 0.475 ± 0.063
0.688CysPhe: 0.688 ± 0.07
0.859CysGly: 0.859 ± 0.072
0.334CysHis: 0.334 ± 0.048
1.76CysIle: 1.76 ± 0.117
1.001CysLys: 1.001 ± 0.095
1.263CysLeu: 1.263 ± 0.107
0.241CysMet: 0.241 ± 0.036
1.135CysAsn: 1.135 ± 0.084
0.461CysPro: 0.461 ± 0.055
0.568CysGln: 0.568 ± 0.055
0.326CysArg: 0.326 ± 0.046
0.958CysSer: 0.958 ± 0.077
0.681CysThr: 0.681 ± 0.069
0.674CysVal: 0.674 ± 0.067
0.135CysTrp: 0.135 ± 0.029
0.603CysTyr: 0.603 ± 0.066
0.0CysXaa: 0.0 ± 0.0
Asp
1.731AspAla: 1.731 ± 0.129
0.305AspCys: 0.305 ± 0.042
1.362AspAsp: 1.362 ± 0.104
1.256AspGlu: 1.256 ± 0.098
1.682AspPhe: 1.682 ± 0.107
1.675AspGly: 1.675 ± 0.127
1.015AspHis: 1.015 ± 0.084
6.983AspIle: 6.983 ± 0.255
2.824AspLys: 2.824 ± 0.132
3.548AspLeu: 3.548 ± 0.153
0.965AspMet: 0.965 ± 0.078
3.101AspAsn: 3.101 ± 0.157
1.008AspPro: 1.008 ± 0.076
1.093AspGln: 1.093 ± 0.084
1.064AspArg: 1.064 ± 0.093
1.582AspSer: 1.582 ± 0.1
1.895AspThr: 1.895 ± 0.117
2.1AspVal: 2.1 ± 0.125
0.291AspTrp: 0.291 ± 0.039
1.909AspTyr: 1.909 ± 0.131
0.0AspXaa: 0.0 ± 0.0
Glu
1.767GluAla: 1.767 ± 0.113
0.334GluCys: 0.334 ± 0.045
1.249GluAsp: 1.249 ± 0.111
1.54GluGlu: 1.54 ± 0.148
1.284GluPhe: 1.284 ± 0.09
1.625GluGly: 1.625 ± 0.118
1.064GluHis: 1.064 ± 0.087
4.825GluIle: 4.825 ± 0.206
3.867GluLys: 3.867 ± 0.171
3.406GluLeu: 3.406 ± 0.156
0.915GluMet: 0.915 ± 0.076
2.434GluAsn: 2.434 ± 0.136
0.923GluPro: 0.923 ± 0.091
2.015GluGln: 2.015 ± 0.113
1.199GluArg: 1.199 ± 0.101
1.746GluSer: 1.746 ± 0.119
1.909GluThr: 1.909 ± 0.127
1.746GluVal: 1.746 ± 0.124
0.22GluTrp: 0.22 ± 0.04
1.859GluTyr: 1.859 ± 0.107
0.0GluXaa: 0.0 ± 0.0
Phe
1.582PheAla: 1.582 ± 0.106
0.781PheCys: 0.781 ± 0.075
1.731PheAsp: 1.731 ± 0.103
1.213PheGlu: 1.213 ± 0.097
3.037PhePhe: 3.037 ± 0.172
2.122PheGly: 2.122 ± 0.114
1.327PheHis: 1.327 ± 0.089
6.678PheIle: 6.678 ± 0.261
3.413PheLys: 3.413 ± 0.134
5.436PheLeu: 5.436 ± 0.239
1.001PheMet: 1.001 ± 0.081
3.789PheAsn: 3.789 ± 0.157
1.171PhePro: 1.171 ± 0.093
1.433PheGln: 1.433 ± 0.115
1.001PheArg: 1.001 ± 0.09
2.909PheSer: 2.909 ± 0.157
2.619PheThr: 2.619 ± 0.161
1.611PheVal: 1.611 ± 0.12
0.518PheTrp: 0.518 ± 0.056
2.484PheTyr: 2.484 ± 0.163
0.0PheXaa: 0.0 ± 0.0
Gly
2.399GlyAla: 2.399 ± 0.164
0.894GlyCys: 0.894 ± 0.09
1.909GlyAsp: 1.909 ± 0.142
1.923GlyGlu: 1.923 ± 0.147
2.1GlyPhe: 2.1 ± 0.124
3.009GlyGly: 3.009 ± 0.194
1.611GlyHis: 1.611 ± 0.119
6.777GlyIle: 6.777 ± 0.236
3.718GlyLys: 3.718 ± 0.204
4.435GlyLeu: 4.435 ± 0.204
1.284GlyMet: 1.284 ± 0.108
2.526GlyAsn: 2.526 ± 0.138
1.178GlyPro: 1.178 ± 0.088
1.497GlyGln: 1.497 ± 0.116
1.632GlyArg: 1.632 ± 0.115
2.64GlySer: 2.64 ± 0.14
2.533GlyThr: 2.533 ± 0.145
2.406GlyVal: 2.406 ± 0.147
0.518GlyTrp: 0.518 ± 0.062
2.413GlyTyr: 2.413 ± 0.147
0.0GlyXaa: 0.0 ± 0.0
His
1.256HisAla: 1.256 ± 0.076
0.369HisCys: 0.369 ± 0.047
1.348HisAsp: 1.348 ± 0.092
0.88HisGlu: 0.88 ± 0.073
1.206HisPhe: 1.206 ± 0.096
1.554HisGly: 1.554 ± 0.103
0.901HisHis: 0.901 ± 0.084
4.669HisIle: 4.669 ± 0.189
3.108HisLys: 3.108 ± 0.154
2.384HisLeu: 2.384 ± 0.139
0.617HisMet: 0.617 ± 0.065
3.179HisAsn: 3.179 ± 0.147
1.036HisPro: 1.036 ± 0.09
0.986HisGln: 0.986 ± 0.085
0.915HisArg: 0.915 ± 0.091
1.299HisSer: 1.299 ± 0.094
1.554HisThr: 1.554 ± 0.101
1.341HisVal: 1.341 ± 0.092
0.199HisTrp: 0.199 ± 0.039
1.526HisTyr: 1.526 ± 0.105
0.0HisXaa: 0.0 ± 0.0
Ile
5.947IleAla: 5.947 ± 0.231
1.994IleCys: 1.994 ± 0.128
5.62IleAsp: 5.62 ± 0.206
4.868IleGlu: 4.868 ± 0.19
6.607IlePhe: 6.607 ± 0.264
6.706IleGly: 6.706 ± 0.251
4.471IleHis: 4.471 ± 0.179
19.692IleIle: 19.692 ± 0.5
12.504IleLys: 12.504 ± 0.342
14.895IleLeu: 14.895 ± 0.427
3.52IleMet: 3.52 ± 0.15
13.0IleAsn: 13.0 ± 0.373
4.584IlePro: 4.584 ± 0.173
5.925IleGln: 5.925 ± 0.202
3.726IleArg: 3.726 ± 0.198
8.53IleSer: 8.53 ± 0.241
8.367IleThr: 8.367 ± 0.224
5.407IleVal: 5.407 ± 0.238
0.965IleTrp: 0.965 ± 0.092
6.436IleTyr: 6.436 ± 0.273
0.0IleXaa: 0.0 ± 0.0
Lys
2.498LysAla: 2.498 ± 0.132
0.802LysCys: 0.802 ± 0.083
3.066LysAsp: 3.066 ± 0.161
3.357LysGlu: 3.357 ± 0.166
3.903LysPhe: 3.903 ± 0.189
2.902LysGly: 2.902 ± 0.159
3.058LysHis: 3.058 ± 0.152
14.193LysIle: 14.193 ± 0.405
12.66LysLys: 12.66 ± 0.372
7.636LysLeu: 7.636 ± 0.228
2.242LysMet: 2.242 ± 0.12
10.474LysAsn: 10.474 ± 0.287
1.838LysPro: 1.838 ± 0.112
4.911LysGln: 4.911 ± 0.191
2.512LysArg: 2.512 ± 0.132
3.442LysSer: 3.442 ± 0.144
5.017LysThr: 5.017 ± 0.204
2.661LysVal: 2.661 ± 0.128
0.582LysTrp: 0.582 ± 0.072
6.188LysTyr: 6.188 ± 0.278
0.0LysXaa: 0.0 ± 0.0
Leu
3.647LeuAla: 3.647 ± 0.175
1.767LeuCys: 1.767 ± 0.145
3.797LeuAsp: 3.797 ± 0.166
3.988LeuGlu: 3.988 ± 0.195
4.655LeuPhe: 4.655 ± 0.224
4.939LeuGly: 4.939 ± 0.19
3.129LeuHis: 3.129 ± 0.147
10.581LeuIle: 10.581 ± 0.32
8.409LeuLys: 8.409 ± 0.26
10.417LeuLeu: 10.417 ± 0.432
2.022LeuMet: 2.022 ± 0.115
7.43LeuAsn: 7.43 ± 0.239
3.428LeuPro: 3.428 ± 0.144
4.648LeuGln: 4.648 ± 0.173
2.931LeuArg: 2.931 ± 0.131
6.777LeuSer: 6.777 ± 0.233
4.634LeuThr: 4.634 ± 0.183
3.711LeuVal: 3.711 ± 0.13
0.944LeuTrp: 0.944 ± 0.092
5.301LeuTyr: 5.301 ± 0.248
0.0LeuXaa: 0.0 ± 0.0
Met
0.951MetAla: 0.951 ± 0.076
0.305MetCys: 0.305 ± 0.043
0.873MetAsp: 0.873 ± 0.093
0.944MetGlu: 0.944 ± 0.08
1.213MetPhe: 1.213 ± 0.103
1.079MetGly: 1.079 ± 0.099
0.674MetHis: 0.674 ± 0.066
2.704MetIle: 2.704 ± 0.15
1.923MetLys: 1.923 ± 0.108
2.633MetLeu: 2.633 ± 0.134
0.525MetMet: 0.525 ± 0.056
1.951MetAsn: 1.951 ± 0.109
0.802MetPro: 0.802 ± 0.086
1.121MetGln: 1.121 ± 0.079
0.745MetArg: 0.745 ± 0.065
1.554MetSer: 1.554 ± 0.118
0.986MetThr: 0.986 ± 0.092
0.937MetVal: 0.937 ± 0.086
0.099MetTrp: 0.099 ± 0.027
1.093MetTyr: 1.093 ± 0.098
0.0MetXaa: 0.0 ± 0.0
Asn
2.54AsnAla: 2.54 ± 0.136
0.83AsnCys: 0.83 ± 0.084
2.782AsnAsp: 2.782 ± 0.132
2.022AsnGlu: 2.022 ± 0.117
4.605AsnPhe: 4.605 ± 0.19
2.59AsnGly: 2.59 ± 0.165
2.157AsnHis: 2.157 ± 0.129
18.28AsnIle: 18.28 ± 0.47
8.877AsnLys: 8.877 ± 0.294
6.685AsnLeu: 6.685 ± 0.233
2.157AsnMet: 2.157 ± 0.109
10.566AsnAsn: 10.566 ± 0.326
2.093AsnPro: 2.093 ± 0.114
2.526AsnGln: 2.526 ± 0.118
1.888AsnArg: 1.888 ± 0.1
3.42AsnSer: 3.42 ± 0.143
4.868AsnThr: 4.868 ± 0.218
3.3AsnVal: 3.3 ± 0.157
0.596AsnTrp: 0.596 ± 0.063
3.931AsnTyr: 3.931 ± 0.17
0.0AsnXaa: 0.0 ± 0.0
Pro
1.001ProAla: 1.001 ± 0.087
0.326ProCys: 0.326 ± 0.048
0.93ProAsp: 0.93 ± 0.087
1.377ProGlu: 1.377 ± 0.104
1.05ProPhe: 1.05 ± 0.083
1.831ProGly: 1.831 ± 0.117
0.859ProHis: 0.859 ± 0.077
4.173ProIle: 4.173 ± 0.165
2.363ProLys: 2.363 ± 0.132
2.718ProLeu: 2.718 ± 0.136
0.695ProMet: 0.695 ± 0.065
2.051ProAsn: 2.051 ± 0.125
0.724ProPro: 0.724 ± 0.076
1.072ProGln: 1.072 ± 0.096
0.71ProArg: 0.71 ± 0.076
1.412ProSer: 1.412 ± 0.083
1.597ProThr: 1.597 ± 0.101
1.419ProVal: 1.419 ± 0.108
0.284ProTrp: 0.284 ± 0.048
1.639ProTyr: 1.639 ± 0.106
0.0ProXaa: 0.0 ± 0.0
Gln
1.731GlnAla: 1.731 ± 0.115
0.603GlnCys: 0.603 ± 0.061
1.604GlnAsp: 1.604 ± 0.109
1.881GlnGlu: 1.881 ± 0.126
1.476GlnPhe: 1.476 ± 0.11
1.817GlnGly: 1.817 ± 0.116
1.668GlnHis: 1.668 ± 0.095
4.591GlnIle: 4.591 ± 0.194
4.769GlnLys: 4.769 ± 0.197
3.697GlnLeu: 3.697 ± 0.178
0.717GlnMet: 0.717 ± 0.062
3.974GlnAsn: 3.974 ± 0.159
0.972GlnPro: 0.972 ± 0.093
2.42GlnGln: 2.42 ± 0.134
1.1GlnArg: 1.1 ± 0.104
1.731GlnSer: 1.731 ± 0.11
2.015GlnThr: 2.015 ± 0.11
1.355GlnVal: 1.355 ± 0.108
0.248GlnTrp: 0.248 ± 0.044
2.909GlnTyr: 2.909 ± 0.149
0.0GlnXaa: 0.0 ± 0.0
Arg
1.455ArgAla: 1.455 ± 0.094
0.348ArgCys: 0.348 ± 0.053
1.015ArgAsp: 1.015 ± 0.092
1.192ArgGlu: 1.192 ± 0.109
1.228ArgPhe: 1.228 ± 0.1
1.448ArgGly: 1.448 ± 0.115
0.639ArgHis: 0.639 ± 0.068
3.889ArgIle: 3.889 ± 0.194
2.498ArgLys: 2.498 ± 0.139
2.626ArgLeu: 2.626 ± 0.138
0.731ArgMet: 0.731 ± 0.074
2.093ArgAsn: 2.093 ± 0.128
0.944ArgPro: 0.944 ± 0.076
1.256ArgGln: 1.256 ± 0.091
1.072ArgArg: 1.072 ± 0.094
1.753ArgSer: 1.753 ± 0.125
1.441ArgThr: 1.441 ± 0.094
1.157ArgVal: 1.157 ± 0.111
0.234ArgTrp: 0.234 ± 0.043
1.433ArgTyr: 1.433 ± 0.08
0.0ArgXaa: 0.0 ± 0.0
Ser
2.427SerAla: 2.427 ± 0.137
0.759SerCys: 0.759 ± 0.067
1.788SerAsp: 1.788 ± 0.104
1.746SerGlu: 1.746 ± 0.127
2.732SerPhe: 2.732 ± 0.148
3.094SerGly: 3.094 ± 0.169
1.476SerHis: 1.476 ± 0.089
7.728SerIle: 7.728 ± 0.221
4.57SerLys: 4.57 ± 0.189
5.876SerLeu: 5.876 ± 0.213
1.292SerMet: 1.292 ± 0.094
3.733SerAsn: 3.733 ± 0.147
1.143SerPro: 1.143 ± 0.073
1.916SerGln: 1.916 ± 0.117
1.675SerArg: 1.675 ± 0.109
3.385SerSer: 3.385 ± 0.135
2.668SerThr: 2.668 ± 0.131
2.157SerVal: 2.157 ± 0.13
0.589SerTrp: 0.589 ± 0.055
2.675SerTyr: 2.675 ± 0.144
0.0SerXaa: 0.0 ± 0.0
Thr
2.037ThrAla: 2.037 ± 0.134
0.681ThrCys: 0.681 ± 0.063
2.1ThrAsp: 2.1 ± 0.137
2.186ThrGlu: 2.186 ± 0.124
2.179ThrPhe: 2.179 ± 0.123
3.137ThrGly: 3.137 ± 0.169
1.611ThrHis: 1.611 ± 0.119
7.004ThrIle: 7.004 ± 0.187
5.06ThrLys: 5.06 ± 0.218
5.244ThrLeu: 5.244 ± 0.174
1.093ThrMet: 1.093 ± 0.082
4.527ThrAsn: 4.527 ± 0.178
1.788ThrPro: 1.788 ± 0.106
2.306ThrGln: 2.306 ± 0.131
1.512ThrArg: 1.512 ± 0.107
2.775ThrSer: 2.775 ± 0.149
3.293ThrThr: 3.293 ± 0.151
2.285ThrVal: 2.285 ± 0.148
0.504ThrTrp: 0.504 ± 0.062
2.377ThrTyr: 2.377 ± 0.146
0.0ThrXaa: 0.0 ± 0.0
Val
1.717ValAla: 1.717 ± 0.157
0.646ValCys: 0.646 ± 0.079
1.604ValAsp: 1.604 ± 0.104
1.597ValGlu: 1.597 ± 0.107
1.703ValPhe: 1.703 ± 0.111
1.973ValGly: 1.973 ± 0.116
1.128ValHis: 1.128 ± 0.097
5.407ValIle: 5.407 ± 0.163
3.491ValLys: 3.491 ± 0.191
4.364ValLeu: 4.364 ± 0.204
1.015ValMet: 1.015 ± 0.087
2.831ValAsn: 2.831 ± 0.173
1.426ValPro: 1.426 ± 0.095
1.426ValGln: 1.426 ± 0.103
1.277ValArg: 1.277 ± 0.085
2.349ValSer: 2.349 ± 0.118
2.604ValThr: 2.604 ± 0.131
1.937ValVal: 1.937 ± 0.121
0.298ValTrp: 0.298 ± 0.044
1.625ValTyr: 1.625 ± 0.134
0.0ValXaa: 0.0 ± 0.0
Trp
0.22TrpAla: 0.22 ± 0.044
0.17TrpCys: 0.17 ± 0.037
0.348TrpAsp: 0.348 ± 0.052
0.49TrpGlu: 0.49 ± 0.059
0.426TrpPhe: 0.426 ± 0.055
0.326TrpGly: 0.326 ± 0.055
0.27TrpHis: 0.27 ± 0.039
1.05TrpIle: 1.05 ± 0.107
0.653TrpLys: 0.653 ± 0.075
0.979TrpLeu: 0.979 ± 0.086
0.213TrpMet: 0.213 ± 0.04
0.596TrpAsn: 0.596 ± 0.061
0.255TrpPro: 0.255 ± 0.042
0.348TrpGln: 0.348 ± 0.054
0.248TrpArg: 0.248 ± 0.042
0.39TrpSer: 0.39 ± 0.049
0.284TrpThr: 0.284 ± 0.045
0.27TrpVal: 0.27 ± 0.049
0.078TrpTrp: 0.078 ± 0.023
0.475TrpTyr: 0.475 ± 0.057
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.873TyrAla: 1.873 ± 0.121
0.816TyrCys: 0.816 ± 0.079
2.306TyrAsp: 2.306 ± 0.162
1.377TyrGlu: 1.377 ± 0.12
2.661TyrPhe: 2.661 ± 0.158
2.427TyrGly: 2.427 ± 0.115
1.817TyrHis: 1.817 ± 0.114
7.103TyrIle: 7.103 ± 0.268
4.833TyrLys: 4.833 ± 0.175
4.755TyrLeu: 4.755 ± 0.219
1.022TyrMet: 1.022 ± 0.081
5.166TyrAsn: 5.166 ± 0.211
1.554TyrPro: 1.554 ± 0.099
2.044TyrGln: 2.044 ± 0.138
1.327TyrArg: 1.327 ± 0.096
2.576TyrSer: 2.576 ± 0.146
2.626TyrThr: 2.626 ± 0.158
1.951TyrVal: 1.951 ± 0.11
0.49TyrTrp: 0.49 ± 0.06
2.519TyrTyr: 2.519 ± 0.155
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 415 proteins (140920 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski