Hubei diptera virus 9

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Sigmavirus; Hubei sigmavirus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMW8|A0A1L3KMW8_9VIRU Putative X protein OS=Hubei diptera virus 9 OX=1922889 PE=4 SV=1
MM1 pKa = 7.55NSLNQTRR8 pKa = 11.84VFCTLDD14 pKa = 3.17GKK16 pKa = 10.61FYY18 pKa = 11.02NVSNVDD24 pKa = 3.44DD25 pKa = 4.43SKK27 pKa = 10.66PTSFPSAWFEE37 pKa = 4.17ANRR40 pKa = 11.84GQKK43 pKa = 9.87PKK45 pKa = 10.86LNIMKK50 pKa = 10.02DD51 pKa = 3.53VPLSKK56 pKa = 10.5LRR58 pKa = 11.84ALMLSSPTNPKK69 pKa = 9.44VPVIAVVTYY78 pKa = 10.05LYY80 pKa = 11.02LVLKK84 pKa = 9.26EE85 pKa = 4.63RR86 pKa = 11.84KK87 pKa = 7.91TICPDD92 pKa = 2.83DD93 pKa = 3.51WSSYY97 pKa = 7.34NTSICSANSQISPFDD112 pKa = 3.5LVTIGEE118 pKa = 4.19IEE120 pKa = 4.77AIEE123 pKa = 4.73LDD125 pKa = 4.0TVVEE129 pKa = 4.13RR130 pKa = 11.84DD131 pKa = 3.75LPSGADD137 pKa = 2.88KK138 pKa = 10.58WIIIMVLSIYY148 pKa = 10.16RR149 pKa = 11.84LEE151 pKa = 4.37RR152 pKa = 11.84ARR154 pKa = 11.84EE155 pKa = 3.76PAYY158 pKa = 10.09EE159 pKa = 3.85ATLIRR164 pKa = 11.84NITNLIKK171 pKa = 10.72ACGCTEE177 pKa = 3.93PPNLTSLLRR186 pKa = 11.84LHH188 pKa = 6.8LKK190 pKa = 8.19WANDD194 pKa = 3.87PGFKK198 pKa = 9.58TAVAAIDD205 pKa = 3.22MFYY208 pKa = 11.14YY209 pKa = 10.51RR210 pKa = 11.84FEE212 pKa = 4.3NDD214 pKa = 2.18EE215 pKa = 3.9WADD218 pKa = 3.25IRR220 pKa = 11.84IATIGSRR227 pKa = 11.84LKK229 pKa = 10.72DD230 pKa = 3.42CSAIQSLTQTASIAGLSSTHH250 pKa = 6.74EE251 pKa = 3.98IIYY254 pKa = 9.65WIGNVAVGTEE264 pKa = 4.13FSKK267 pKa = 10.53MSSSVEE273 pKa = 4.01EE274 pKa = 4.08AGMLYY279 pKa = 10.23SYY281 pKa = 10.71FPYY284 pKa = 10.4QVDD287 pKa = 4.27FGLVTRR293 pKa = 11.84SAYY296 pKa = 9.06SARR299 pKa = 11.84VNPALHH305 pKa = 6.05TWLHH309 pKa = 6.06IIGIFFCNSRR319 pKa = 11.84SLNAKK324 pKa = 9.66FFLEE328 pKa = 3.95KK329 pKa = 10.59DD330 pKa = 3.47VAGVIRR336 pKa = 11.84SAQWVGYY343 pKa = 8.33VLSRR347 pKa = 11.84GQHH350 pKa = 4.91SGRR353 pKa = 11.84IFVPADD359 pKa = 3.58RR360 pKa = 11.84PEE362 pKa = 3.81QDD364 pKa = 5.43MVDD367 pKa = 3.47EE368 pKa = 4.44WNEE371 pKa = 3.95NQQEE375 pKa = 4.12ATDD378 pKa = 3.97ANEE381 pKa = 4.09GVEE384 pKa = 4.66DD385 pKa = 4.02LTLGAISDD393 pKa = 3.93TDD395 pKa = 3.65LTRR398 pKa = 11.84EE399 pKa = 4.07PLSNNPFEE407 pKa = 4.38WFSYY411 pKa = 9.29MSHH414 pKa = 7.1RR415 pKa = 11.84DD416 pKa = 3.6YY417 pKa = 11.6KK418 pKa = 9.88LTQSMRR424 pKa = 11.84SKK426 pKa = 10.1IRR428 pKa = 11.84SVQSEE433 pKa = 4.28IVNPRR438 pKa = 11.84PGTIAAMAQTSLFYY452 pKa = 11.07HH453 pKa = 6.78EE454 pKa = 4.83

Molecular weight: 51.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMU2|A0A1L3KMU2_9VIRU Nucleocapsid protein OS=Hubei diptera virus 9 OX=1922889 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.2KK3 pKa = 10.31INMEE7 pKa = 4.31NNNTTKK13 pKa = 10.08MNKK16 pKa = 8.14KK17 pKa = 8.16TPNKK21 pKa = 9.1SQSKK25 pKa = 7.99TRR27 pKa = 11.84STKK30 pKa = 9.09QRR32 pKa = 11.84EE33 pKa = 4.23INPPLLQQFGPNTPQDD49 pKa = 3.52PKK51 pKa = 11.11KK52 pKa = 10.03WFDD55 pKa = 3.49EE56 pKa = 4.43AQSRR60 pKa = 11.84PGEE63 pKa = 3.94MLEE66 pKa = 4.5NVMKK70 pKa = 10.03TLAKK74 pKa = 10.53DD75 pKa = 3.88GPNPEE80 pKa = 3.94AEE82 pKa = 4.06IRR84 pKa = 11.84EE85 pKa = 4.12EE86 pKa = 4.9DD87 pKa = 4.52SGFLNPNIPTGIIDD101 pKa = 5.17DD102 pKa = 5.49PIEE105 pKa = 4.39CMAVGAAALHH115 pKa = 6.04LSEE118 pKa = 5.98FPTTSKK124 pKa = 10.58RR125 pKa = 11.84GRR127 pKa = 11.84EE128 pKa = 3.84VNTYY132 pKa = 8.15KK133 pKa = 10.33TAEE136 pKa = 3.79THH138 pKa = 6.25QYY140 pKa = 7.91VTADD144 pKa = 4.07LIEE147 pKa = 4.63NKK149 pKa = 9.48FQNGQSYY156 pKa = 11.27DD157 pKa = 3.27SDD159 pKa = 3.81NFLMIFKK166 pKa = 10.42KK167 pKa = 10.74LKK169 pKa = 9.82TNMGDD174 pKa = 3.27TADD177 pKa = 4.41KK178 pKa = 10.85YY179 pKa = 11.1FKK181 pKa = 9.94LTEE184 pKa = 4.56GEE186 pKa = 4.45GEE188 pKa = 3.95WLITLKK194 pKa = 10.77NPYY197 pKa = 9.32PSPEE201 pKa = 4.45LKK203 pKa = 10.2RR204 pKa = 11.84QRR206 pKa = 11.84QTEE209 pKa = 4.39CIPTSSKK216 pKa = 5.89QTKK219 pKa = 10.25VIFPDD224 pKa = 3.29EE225 pKa = 4.16NTNQGASEE233 pKa = 4.33TEE235 pKa = 3.88IVNPLLEE242 pKa = 4.96SYY244 pKa = 11.02LKK246 pKa = 10.72FLTPGLTLKK255 pKa = 10.62SRR257 pKa = 11.84EE258 pKa = 4.06YY259 pKa = 11.41GLGSLKK265 pKa = 10.35ISLGSFSMRR274 pKa = 11.84EE275 pKa = 3.67EE276 pKa = 3.8DD277 pKa = 3.29VRR279 pKa = 11.84AYY281 pKa = 10.3FVGKK285 pKa = 9.86EE286 pKa = 3.57IPTTFRR292 pKa = 11.84AFVYY296 pKa = 9.75TLAKK300 pKa = 9.35CQPKK304 pKa = 10.0IIRR307 pKa = 11.84VLNKK311 pKa = 10.17YY312 pKa = 9.46IVPLNVKK319 pKa = 8.77STDD322 pKa = 3.04

Molecular weight: 36.54 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3998

229

2120

666.3

76.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.977 ± 0.701

1.501 ± 0.184

5.078 ± 0.168

6.203 ± 0.244

4.677 ± 0.215

4.852 ± 0.201

2.551 ± 0.47

7.529 ± 0.63

5.628 ± 0.501

10.63 ± 1.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.701 ± 0.185

6.053 ± 0.208

5.078 ± 0.307

3.352 ± 0.235

5.078 ± 0.276

8.579 ± 0.388

6.428 ± 0.468

5.603 ± 0.357

1.726 ± 0.143

3.777 ± 0.399
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski