Lactococcus phage Dub35A

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8BMV6|A0A1P8BMV6_9CAUD Uncharacterized protein OS=Lactococcus phage Dub35A OX=1868862 GN=Dub35A_039 PE=4 SV=1
MM1 pKa = 8.09RR2 pKa = 11.84YY3 pKa = 9.93FNTLTKK9 pKa = 10.14ATIDD13 pKa = 3.43TDD15 pKa = 4.12FKK17 pKa = 11.04ISGGDD22 pKa = 2.93WVLEE26 pKa = 4.14NEE28 pKa = 4.34SNEE31 pKa = 4.24AIVDD35 pKa = 3.76IQANDD40 pKa = 3.74ADD42 pKa = 4.36SKK44 pKa = 11.25KK45 pKa = 10.23PEE47 pKa = 3.84QEE49 pKa = 4.04QVVEE53 pKa = 4.15EE54 pKa = 4.62SNVDD58 pKa = 3.79GNYY61 pKa = 10.98DD62 pKa = 4.32WITKK66 pKa = 8.69DD67 pKa = 4.44QIMQEE72 pKa = 3.78LDD74 pKa = 3.2AFGIRR79 pKa = 11.84YY80 pKa = 8.45DD81 pKa = 3.38KK82 pKa = 10.88RR83 pKa = 11.84ANKK86 pKa = 8.98QVLYY90 pKa = 10.72DD91 pKa = 4.1LMMEE95 pKa = 4.15QGKK98 pKa = 8.58EE99 pKa = 3.75

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8BMT3|A0A1P8BMT3_9CAUD Scaffold protein OS=Lactococcus phage Dub35A OX=1868862 GN=Dub35A_035 PE=4 SV=1
MM1 pKa = 7.68AKK3 pKa = 10.31NLFKK7 pKa = 11.11LNRR10 pKa = 11.84SGVASMMKK18 pKa = 9.93SPEE21 pKa = 3.64MQAILKK27 pKa = 9.14EE28 pKa = 3.99KK29 pKa = 10.66ASAVKK34 pKa = 9.6QRR36 pKa = 11.84CGPGYY41 pKa = 9.68GQDD44 pKa = 2.96MHH46 pKa = 7.79VGKK49 pKa = 10.17NRR51 pKa = 11.84ANAMVFAEE59 pKa = 5.25TYY61 pKa = 7.86QAKK64 pKa = 9.74RR65 pKa = 11.84DD66 pKa = 3.63NMKK69 pKa = 10.98NNTILKK75 pKa = 9.9AVRR78 pKa = 3.37

Molecular weight: 8.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

10642

31

843

200.8

22.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.343 ± 0.477

0.658 ± 0.113

6.183 ± 0.295

6.606 ± 0.491

4.285 ± 0.242

6.587 ± 0.487

1.419 ± 0.167

7.461 ± 0.353

8.419 ± 0.491

8.166 ± 0.275

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.359 ± 0.165

5.976 ± 0.292

3.261 ± 0.178

3.834 ± 0.176

3.749 ± 0.344

7.245 ± 0.475

6.239 ± 0.292

6.127 ± 0.23

1.428 ± 0.117

3.646 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski