Blumeria graminis f. sp. tritici 96224

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Erysiphales; Erysiphaceae; Blumeria; Blumeria graminis; Blumeria graminis f. sp. tritici

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4024 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A656KJQ1|A0A656KJQ1_BLUGR Uncharacterized protein OS=Blumeria graminis f. sp. tritici 96224 OX=1268274 GN=BGT96224_Ac30422 PE=4 SV=1
MM1 pKa = 7.83YY2 pKa = 10.17EE3 pKa = 5.34DD4 pKa = 5.75DD5 pKa = 4.46YY6 pKa = 12.09LDD8 pKa = 3.76INEE11 pKa = 4.73TYY13 pKa = 11.21LDD15 pKa = 3.84LFSNEE20 pKa = 3.64TRR22 pKa = 11.84QVSGGVDD29 pKa = 3.44IIGYY33 pKa = 7.44CFPTQPPQPNGYY45 pKa = 8.23GTEE48 pKa = 4.09CLTYY52 pKa = 8.23TGQVSGVFSDD62 pKa = 4.76DD63 pKa = 3.55PSEE66 pKa = 3.91EE67 pKa = 3.92DD68 pKa = 3.36VPRR71 pKa = 11.84KK72 pKa = 9.85RR73 pKa = 11.84RR74 pKa = 11.84DD75 pKa = 3.59GIGEE79 pKa = 4.03EE80 pKa = 4.38EE81 pKa = 4.37RR82 pKa = 11.84CDD84 pKa = 3.38EE85 pKa = 4.5DD86 pKa = 4.2RR87 pKa = 11.84AGRR90 pKa = 11.84CEE92 pKa = 3.68

Molecular weight: 10.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A656KIX2|A0A656KIX2_BLUGR Uncharacterized protein OS=Blumeria graminis f. sp. tritici 96224 OX=1268274 GN=BGT96224_2832 PE=4 SV=1
MM1 pKa = 7.54GVPPLLSSLAIQGAAKK17 pKa = 10.46AFVHH21 pKa = 5.52GQSRR25 pKa = 11.84ASARR29 pKa = 11.84QSSSDD34 pKa = 3.66SASLTTPALVAARR47 pKa = 11.84ATTVKK52 pKa = 9.5STAKK56 pKa = 10.14NLPASHH62 pKa = 6.74LQPRR66 pKa = 11.84PGDD69 pKa = 3.52APTILGPSRR78 pKa = 11.84PTISTTSHH86 pKa = 5.76VSTPSSRR93 pKa = 11.84SSRR96 pKa = 11.84SSCTQGRR103 pKa = 11.84SAGEE107 pKa = 3.9QASLLYY113 pKa = 10.24RR114 pKa = 11.84PSSLPSSVARR124 pKa = 11.84DD125 pKa = 3.2AAAAAVASTCNLYY138 pKa = 8.41MQPPLSSPCLAPSTITLKK156 pKa = 9.34PTLRR160 pKa = 11.84TSHH163 pKa = 7.04RR164 pKa = 11.84SDD166 pKa = 4.49CEE168 pKa = 3.95DD169 pKa = 3.6SEE171 pKa = 4.57PTCRR175 pKa = 11.84SSAQQRR181 pKa = 11.84RR182 pKa = 11.84SLLHH186 pKa = 6.78PNLHH190 pKa = 4.55QCRR193 pKa = 11.84RR194 pKa = 11.84RR195 pKa = 11.84RR196 pKa = 11.84PMEE199 pKa = 3.85IVTDD203 pKa = 4.12RR204 pKa = 11.84QRR206 pKa = 11.84RR207 pKa = 11.84RR208 pKa = 11.84YY209 pKa = 7.93EE210 pKa = 3.93AVWASNRR217 pKa = 11.84GLYY220 pKa = 10.19LDD222 pKa = 4.23SRR224 pKa = 11.84SHH226 pKa = 5.42EE227 pKa = 3.81AAMRR231 pKa = 11.84LCNLVVRR238 pKa = 11.84DD239 pKa = 3.09IWSRR243 pKa = 11.84SRR245 pKa = 11.84LGSDD249 pKa = 3.3VLHH252 pKa = 7.07CIYY255 pKa = 10.81EE256 pKa = 4.51LIDD259 pKa = 3.88RR260 pKa = 11.84DD261 pKa = 3.52QCGSLRR267 pKa = 11.84RR268 pKa = 11.84DD269 pKa = 3.52EE270 pKa = 5.01FVVGMWLIDD279 pKa = 3.34RR280 pKa = 11.84SLRR283 pKa = 11.84GRR285 pKa = 11.84KK286 pKa = 8.91IPSKK290 pKa = 10.25ISDD293 pKa = 4.47SIWLSVNN300 pKa = 3.07

Molecular weight: 32.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4022

2

4024

1773325

36

4884

440.7

49.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.876 ± 0.033

1.334 ± 0.012

5.408 ± 0.028

6.48 ± 0.037

3.767 ± 0.02

5.79 ± 0.032

2.352 ± 0.016

6.186 ± 0.025

5.955 ± 0.03

9.095 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.11 ± 0.013

4.722 ± 0.021

5.313 ± 0.032

4.089 ± 0.026

5.817 ± 0.026

9.243 ± 0.04

5.914 ± 0.023

5.453 ± 0.025

1.229 ± 0.014

2.794 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski