Pseudoalteromonas phage BS5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 65 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B2ANT1|A0A1B2ANT1_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage BS5 OX=1874539 PE=4 SV=1
MM1 pKa = 7.41SVYY4 pKa = 10.02QVEE7 pKa = 4.71EE8 pKa = 5.02YY9 pKa = 10.28YY10 pKa = 11.11VHH12 pKa = 7.41IVFEE16 pKa = 4.98DD17 pKa = 3.75EE18 pKa = 4.07LSEE21 pKa = 5.21DD22 pKa = 3.51GKK24 pKa = 9.86EE25 pKa = 4.16TLKK28 pKa = 9.24QTIEE32 pKa = 4.12GEE34 pKa = 4.55GYY36 pKa = 10.19SDD38 pKa = 5.54YY39 pKa = 11.12EE40 pKa = 3.92ITDD43 pKa = 3.2NSVTVDD49 pKa = 3.48GVEE52 pKa = 4.25SEE54 pKa = 4.34QEE56 pKa = 4.1GLQLEE61 pKa = 4.47EE62 pKa = 4.7LLSFF66 pKa = 4.97

Molecular weight: 7.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B2ANS2|A0A1B2ANS2_9CAUD Uncharacterized protein OS=Pseudoalteromonas phage BS5 OX=1874539 PE=4 SV=1
MM1 pKa = 7.5WEE3 pKa = 3.7IEE5 pKa = 4.31RR6 pKa = 11.84AVSSYY11 pKa = 10.66FVGRR15 pKa = 11.84LNWANLLSLDD25 pKa = 4.01TKK27 pKa = 10.86RR28 pKa = 11.84ILTTAILRR36 pKa = 11.84WW37 pKa = 3.57

Molecular weight: 4.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

65

0

65

12331

37

869

189.7

21.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.996 ± 0.559

1.306 ± 0.168

6.569 ± 0.301

6.334 ± 0.428

3.876 ± 0.217

7.112 ± 0.433

1.298 ± 0.14

6.496 ± 0.221

7.015 ± 0.53

8.053 ± 0.347

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.211

5.969 ± 0.184

2.782 ± 0.2

4.112 ± 0.307

3.966 ± 0.211

7.404 ± 0.338

6.301 ± 0.395

5.928 ± 0.197

1.184 ± 0.141

3.787 ± 0.244
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski