Lynx canadensis faeces associated genomovirus CL4 71

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; unclassified Genomoviridae

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z5CK53|A0A2Z5CK53_9VIRU Replication-associated protein OS=Lynx canadensis faeces associated genomovirus CL4 71 OX=2219131 PE=3 SV=1
MM1 pKa = 7.89PSFHH5 pKa = 6.86LKK7 pKa = 8.75NRR9 pKa = 11.84RR10 pKa = 11.84YY11 pKa = 10.69VLFTYY16 pKa = 10.1AQAGSDD22 pKa = 3.66FDD24 pKa = 3.31HH25 pKa = 6.81WAVVDD30 pKa = 4.09LLGGLGAEE38 pKa = 4.56CIIGRR43 pKa = 11.84EE44 pKa = 4.06SHH46 pKa = 6.95ADD48 pKa = 3.3GGVHH52 pKa = 5.47FHH54 pKa = 6.24VFADD58 pKa = 4.63FGRR61 pKa = 11.84LFSTRR66 pKa = 11.84KK67 pKa = 8.13TDD69 pKa = 3.59LFDD72 pKa = 4.25VGGKK76 pKa = 9.29HH77 pKa = 6.83PNIQPIGRR85 pKa = 11.84TPAKK89 pKa = 10.3AYY91 pKa = 10.39DD92 pKa = 3.93YY93 pKa = 10.86ACKK96 pKa = 10.72DD97 pKa = 3.23GDD99 pKa = 4.09VVAGGLGRR107 pKa = 11.84PGGDD111 pKa = 3.43ADD113 pKa = 4.94FDD115 pKa = 4.67PDD117 pKa = 4.67SFWDD121 pKa = 3.7SATHH125 pKa = 6.59CGSADD130 pKa = 3.65EE131 pKa = 4.98FLHH134 pKa = 7.02FCDD137 pKa = 4.14QLAPRR142 pKa = 11.84DD143 pKa = 4.09LIRR146 pKa = 11.84GFPAFRR152 pKa = 11.84SYY154 pKa = 11.85SNWKK158 pKa = 8.47WNDD161 pKa = 2.84GALVYY166 pKa = 9.43NQPDD170 pKa = 3.52GVEE173 pKa = 4.29FDD175 pKa = 3.38TSAAEE180 pKa = 4.92GIDD183 pKa = 3.48EE184 pKa = 4.26RR185 pKa = 11.84RR186 pKa = 11.84SLVLFGPYY194 pKa = 10.43GCGKK198 pKa = 5.65TTWARR203 pKa = 11.84SLGEE207 pKa = 4.02HH208 pKa = 6.89IYY210 pKa = 10.68FGSQWSGKK218 pKa = 7.76VAFQGLEE225 pKa = 3.77KK226 pKa = 10.81AEE228 pKa = 3.85YY229 pKa = 10.37AIFDD233 pKa = 3.71DD234 pKa = 3.95WKK236 pKa = 10.84GGLKK240 pKa = 9.14MLPGYY245 pKa = 10.21KK246 pKa = 9.65DD247 pKa = 2.9WLGAQWHH254 pKa = 5.04VSVRR258 pKa = 11.84QLHH261 pKa = 6.14HH262 pKa = 7.14DD263 pKa = 3.37ARR265 pKa = 11.84LEE267 pKa = 3.93EE268 pKa = 4.12WGRR271 pKa = 11.84PCIWLCNTDD280 pKa = 3.88PRR282 pKa = 11.84VMTHH286 pKa = 6.19VNDD289 pKa = 3.84DD290 pKa = 4.22TDD292 pKa = 3.91WTWMDD297 pKa = 3.94RR298 pKa = 11.84ACIFVEE304 pKa = 4.36VTGVLATFRR313 pKa = 11.84ASTEE317 pKa = 3.78

Molecular weight: 35.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z5CK53|A0A2Z5CK53_9VIRU Replication-associated protein OS=Lynx canadensis faeces associated genomovirus CL4 71 OX=2219131 PE=3 SV=1
MM1 pKa = 7.82AYY3 pKa = 10.14ARR5 pKa = 11.84RR6 pKa = 11.84PRR8 pKa = 11.84RR9 pKa = 11.84PAYY12 pKa = 9.27RR13 pKa = 11.84KK14 pKa = 9.61KK15 pKa = 10.2GARR18 pKa = 11.84STRR21 pKa = 11.84RR22 pKa = 11.84YY23 pKa = 9.83SKK25 pKa = 10.25RR26 pKa = 11.84SYY28 pKa = 10.02PRR30 pKa = 11.84SSYY33 pKa = 10.09VKK35 pKa = 9.18RR36 pKa = 11.84RR37 pKa = 11.84PYY39 pKa = 10.0RR40 pKa = 11.84KK41 pKa = 9.87RR42 pKa = 11.84MTKK45 pKa = 10.34KK46 pKa = 10.35SILNVTSRR54 pKa = 11.84KK55 pKa = 9.78KK56 pKa = 10.11RR57 pKa = 11.84DD58 pKa = 2.95TMAYY62 pKa = 6.99YY63 pKa = 10.34TNSTAASQTGGTTYY77 pKa = 10.72VQDD80 pKa = 3.58AAVVTGGTSVPACFLWCATGRR101 pKa = 11.84DD102 pKa = 4.19YY103 pKa = 8.2TTSNAGTNGQIVDD116 pKa = 3.52AASRR120 pKa = 11.84TSQSCYY126 pKa = 8.3MRR128 pKa = 11.84GLKK131 pKa = 9.53EE132 pKa = 3.77ALEE135 pKa = 4.29VQVADD140 pKa = 3.88GVPWQWRR147 pKa = 11.84RR148 pKa = 11.84ICFTAKK154 pKa = 10.43GLQTLFAATTGFSLVIEE171 pKa = 4.8ASTGYY176 pKa = 10.19QRR178 pKa = 11.84VLNQIPGDD186 pKa = 3.97SATATSLRR194 pKa = 11.84AVIEE198 pKa = 4.14TVLFKK203 pKa = 10.78GVYY206 pKa = 9.22LRR208 pKa = 11.84DD209 pKa = 3.7WIDD212 pKa = 3.43PMTASTDD219 pKa = 3.35NDD221 pKa = 3.79RR222 pKa = 11.84LTIKK226 pKa = 10.24YY227 pKa = 9.92DD228 pKa = 3.27KK229 pKa = 10.2TITIASGNEE238 pKa = 3.72DD239 pKa = 3.41GCIRR243 pKa = 11.84NYY245 pKa = 10.33KK246 pKa = 9.06RR247 pKa = 11.84WHH249 pKa = 6.12GMNKK253 pKa = 8.33TLVYY257 pKa = 10.85NDD259 pKa = 4.72DD260 pKa = 3.63EE261 pKa = 5.35SGGTKK266 pKa = 10.0VPAFYY271 pKa = 9.7STLGKK276 pKa = 10.56AGMGDD281 pKa = 4.51FYY283 pKa = 11.06IMDD286 pKa = 4.19YY287 pKa = 10.35FRR289 pKa = 11.84PRR291 pKa = 11.84AGSTSTNQLRR301 pKa = 11.84VSASSTLYY309 pKa = 9.07WHH311 pKa = 7.06EE312 pKa = 4.1KK313 pKa = 9.32

Molecular weight: 35.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

630

313

317

315.0

35.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.889 ± 0.253

2.063 ± 0.313

7.46 ± 1.577

3.175 ± 0.63

4.603 ± 1.375

9.365 ± 1.14

2.381 ± 1.17

3.492 ± 0.23

4.921 ± 0.772

6.19 ± 0.724

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.905 ± 0.437

2.857 ± 0.441

3.651 ± 0.306

2.857 ± 0.227

7.778 ± 1.214

6.825 ± 1.209

7.937 ± 2.18

5.873 ± 0.082

3.016 ± 0.738

4.762 ± 1.093
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski