Streptococcus phage TP-J34

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L0P538|L0P538_9CAUD Uncharacterized protein OS=Streptococcus phage TP-J34 OX=73422 PE=4 SV=1
MM1 pKa = 7.52SVQQSIVNWFVNHH14 pKa = 6.82RR15 pKa = 11.84GKK17 pKa = 9.04LTYY20 pKa = 11.06SMYY23 pKa = 10.84GSRR26 pKa = 11.84NGSDD30 pKa = 2.98GTADD34 pKa = 3.56CSGSMSQALKK44 pKa = 10.31EE45 pKa = 3.98AGIPIQGLPSTVTLGQQLAKK65 pKa = 10.58NGFYY69 pKa = 10.09RR70 pKa = 11.84VSRR73 pKa = 11.84NEE75 pKa = 3.74DD76 pKa = 2.54WDD78 pKa = 4.06ALMGDD83 pKa = 3.72IVMMSWGADD92 pKa = 3.28MASSGGAGGHH102 pKa = 5.37VGVMIDD108 pKa = 3.25SVNFISCDD116 pKa = 3.3YY117 pKa = 8.82STQGAVGQAINTYY130 pKa = 8.91PWNDD134 pKa = 3.08YY135 pKa = 10.1YY136 pKa = 11.51AANKK140 pKa = 6.83PTYY143 pKa = 9.85IEE145 pKa = 3.7VWRR148 pKa = 11.84YY149 pKa = 10.07ADD151 pKa = 5.12SAPQTNNKK159 pKa = 9.87ANTAVVTQEE168 pKa = 3.59KK169 pKa = 10.29AYY171 pKa = 11.09YY172 pKa = 8.32EE173 pKa = 4.29ANEE176 pKa = 3.96VKK178 pKa = 10.25YY179 pKa = 11.21VNGIWQIRR187 pKa = 11.84CDD189 pKa = 4.05YY190 pKa = 7.54LTPIGFDD197 pKa = 2.87WTEE200 pKa = 3.85NGVPVDD206 pKa = 3.52MVNWVDD212 pKa = 4.54ANGNDD217 pKa = 4.58LPDD220 pKa = 4.62GEE222 pKa = 5.21SKK224 pKa = 10.82DD225 pKa = 4.46FKK227 pKa = 10.99AGMYY231 pKa = 10.19FSFSDD236 pKa = 3.95DD237 pKa = 3.15EE238 pKa = 4.62TNIVDD243 pKa = 3.86TGNGGYY249 pKa = 10.4YY250 pKa = 9.73GGYY253 pKa = 9.69YY254 pKa = 9.72YY255 pKa = 10.64RR256 pKa = 11.84QFEE259 pKa = 4.29FGQFGPVWLSCWNKK273 pKa = 10.66DD274 pKa = 3.89DD275 pKa = 5.14LVNYY279 pKa = 7.38YY280 pKa = 8.26QQ281 pKa = 4.22

Molecular weight: 31.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L0P2T3|L0P2T3_9CAUD Uncharacterized protein OS=Streptococcus phage TP-J34 OX=73422 PE=4 SV=1
MM1 pKa = 7.83ADD3 pKa = 3.75FGVSLLEE10 pKa = 4.01ARR12 pKa = 11.84RR13 pKa = 11.84MTLKK17 pKa = 10.49EE18 pKa = 3.53MKK20 pKa = 9.92LYY22 pKa = 10.32QKK24 pKa = 10.55AYY26 pKa = 9.84KK27 pKa = 10.03KK28 pKa = 10.52RR29 pKa = 11.84FLNKK33 pKa = 9.43EE34 pKa = 3.69RR35 pKa = 11.84EE36 pKa = 4.36IYY38 pKa = 9.68QLAYY42 pKa = 10.87LNRR45 pKa = 11.84LANATTKK52 pKa = 10.62DD53 pKa = 3.03GKK55 pKa = 10.64KK56 pKa = 10.46YY57 pKa = 10.49YY58 pKa = 10.19FEE60 pKa = 5.48KK61 pKa = 10.83FDD63 pKa = 3.95DD64 pKa = 4.49FYY66 pKa = 11.56NAKK69 pKa = 9.54EE70 pKa = 3.83RR71 pKa = 11.84ARR73 pKa = 11.84EE74 pKa = 3.88VLGEE78 pKa = 4.45RR79 pKa = 11.84ITNSKK84 pKa = 10.22LLEE87 pKa = 4.03RR88 pKa = 11.84AKK90 pKa = 11.27NNLNYY95 pKa = 10.4KK96 pKa = 9.81LEE98 pKa = 4.57RR99 pKa = 11.84GLLDD103 pKa = 3.37GRR105 pKa = 4.46

Molecular weight: 12.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

1

60

14182

46

1647

236.4

26.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.263 ± 0.838

0.55 ± 0.091

6.353 ± 0.399

6.79 ± 0.705

3.892 ± 0.199

7.058 ± 0.659

1.297 ± 0.157

6.741 ± 0.413

7.693 ± 0.489

7.538 ± 0.425

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.233

5.867 ± 0.306

3.081 ± 0.374

4.174 ± 0.238

4.195 ± 0.283

6.96 ± 0.542

6.536 ± 0.36

5.994 ± 0.273

1.382 ± 0.222

4.294 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski