Lactococcus phage 5205F

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ceduovirus; unclassified Ceduovirus

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A650ER10|A0A650ER10_9CAUD Uncharacterized protein OS=Lactococcus phage 5205F OX=2675236 GN=5205F_000051 PE=4 SV=1
MM1 pKa = 7.66AEE3 pKa = 3.91KK4 pKa = 10.59NIYY7 pKa = 9.19FVNDD11 pKa = 3.28EE12 pKa = 4.44VEE14 pKa = 4.24LKK16 pKa = 10.42QVLEE20 pKa = 5.24FIDD23 pKa = 3.55KK24 pKa = 10.01TDD26 pKa = 3.39YY27 pKa = 11.08GVNIDD32 pKa = 3.61KK33 pKa = 10.05TRR35 pKa = 11.84EE36 pKa = 3.6DD37 pKa = 3.69VYY39 pKa = 11.64AVVTSYY45 pKa = 11.55SLPII49 pKa = 4.27

Molecular weight: 5.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A650ER20|A0A650ER20_9CAUD Uncharacterized protein OS=Lactococcus phage 5205F OX=2675236 GN=5205F_000063 PE=4 SV=1
MM1 pKa = 7.41ATTNKK6 pKa = 8.11VTGLEE11 pKa = 4.14KK12 pKa = 10.14FTEE15 pKa = 4.18KK16 pKa = 10.32QLKK19 pKa = 9.46KK20 pKa = 10.55VWLEE24 pKa = 3.86MVDD27 pKa = 4.27SFNSNQNTVKK37 pKa = 10.51RR38 pKa = 11.84SYY40 pKa = 10.61KK41 pKa = 10.29SSLGGDD47 pKa = 4.01FSRR50 pKa = 11.84YY51 pKa = 8.21PVTRR55 pKa = 11.84NGRR58 pKa = 3.44

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

35

0

35

6276

46

638

179.3

20.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.326 ± 0.475

0.94 ± 0.199

6.103 ± 0.282

7.138 ± 0.539

4.557 ± 0.293

6.915 ± 0.49

1.179 ± 0.173

6.214 ± 0.415

8.907 ± 0.452

8.222 ± 0.476

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.9 ± 0.254

6.676 ± 0.344

2.597 ± 0.33

4.079 ± 0.319

3.394 ± 0.276

5.577 ± 0.427

6.437 ± 0.489

5.959 ± 0.325

1.546 ± 0.192

4.334 ± 0.342
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski