Enterococcus phage heks

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Efquatrovirus; unclassified Efquatrovirus

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 64 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G9LKK8|A0A6G9LKK8_9CAUD Uncharacterized protein OS=Enterococcus phage heks OX=2719592 GN=heks_60 PE=4 SV=1
MM1 pKa = 7.38YY2 pKa = 9.69IVNIIDD8 pKa = 3.68FEE10 pKa = 4.41ARR12 pKa = 11.84TLAYY16 pKa = 9.8QSEE19 pKa = 4.76VFTTKK24 pKa = 10.31EE25 pKa = 3.29LAEE28 pKa = 4.2NYY30 pKa = 9.28MEE32 pKa = 4.19EE33 pKa = 4.3VMEE36 pKa = 5.0NIHH39 pKa = 6.38GLDD42 pKa = 3.36VLIIEE47 pKa = 4.46VNSYY51 pKa = 10.57NLEE54 pKa = 3.87TAKK57 pKa = 10.33IVACSS62 pKa = 3.15

Molecular weight: 7.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G9LKT3|A0A6G9LKT3_9CAUD Head-tail joining protein OS=Enterococcus phage heks OX=2719592 GN=heks_25 PE=4 SV=1
MM1 pKa = 7.84PAPKK5 pKa = 9.78PIMVKK10 pKa = 10.03DD11 pKa = 3.87LRR13 pKa = 11.84TQQTRR18 pKa = 11.84VFKK21 pKa = 10.69SQSEE25 pKa = 3.86ADD27 pKa = 3.15RR28 pKa = 11.84FYY30 pKa = 11.36NKK32 pKa = 9.97KK33 pKa = 9.9SGYY36 pKa = 10.0FKK38 pKa = 10.66DD39 pKa = 3.7VKK41 pKa = 10.21TKK43 pKa = 10.55LGGKK47 pKa = 8.0NRR49 pKa = 11.84HH50 pKa = 5.09YY51 pKa = 10.77EE52 pKa = 4.06IIQVVV57 pKa = 3.15

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

64

0

64

11984

53

1295

187.3

21.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.766 ± 0.497

0.801 ± 0.13

6.225 ± 0.286

8.344 ± 0.503

3.88 ± 0.252

6.317 ± 0.623

1.535 ± 0.182

6.676 ± 0.187

8.653 ± 0.364

8.57 ± 0.314

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.996 ± 0.196

6.442 ± 0.28

2.854 ± 0.209

3.788 ± 0.225

3.688 ± 0.193

5.224 ± 0.229

6.066 ± 0.364

6.6 ± 0.277

1.318 ± 0.152

4.256 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski