Sierra dome spider associated circular virus 2

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A346BPD5|A0A346BPD5_9VIRU Putative capsid protein OS=Sierra dome spider associated circular virus 2 OX=2293300 PE=4 SV=1
MM1 pKa = 7.56CLDD4 pKa = 4.71LLTVSNMSRR13 pKa = 11.84CRR15 pKa = 11.84NYY17 pKa = 10.3VFTLNNPQGTPMFVPDD33 pKa = 3.14KK34 pKa = 8.99MKK36 pKa = 10.95YY37 pKa = 8.59MVYY40 pKa = 10.1QKK42 pKa = 10.93EE43 pKa = 4.25SGEE46 pKa = 4.18NGTNHH51 pKa = 4.87YY52 pKa = 9.79QGYY55 pKa = 9.53VEE57 pKa = 5.06LLSPTGMPSVKK68 pKa = 10.19RR69 pKa = 11.84MIGQNPHH76 pKa = 6.43LEE78 pKa = 4.22PRR80 pKa = 11.84RR81 pKa = 11.84GTQAEE86 pKa = 3.78ARR88 pKa = 11.84AYY90 pKa = 10.29CMKK93 pKa = 10.5PEE95 pKa = 3.97TRR97 pKa = 11.84VDD99 pKa = 4.19GPWEE103 pKa = 3.9MGTFTEE109 pKa = 4.17NNQGKK114 pKa = 7.88RR115 pKa = 11.84TDD117 pKa = 4.03LEE119 pKa = 4.26LAIQDD124 pKa = 3.41IDD126 pKa = 4.17GGSSMARR133 pKa = 11.84VALDD137 pKa = 3.36HH138 pKa = 6.82PVTFVKK144 pKa = 10.58YY145 pKa = 9.76PRR147 pKa = 11.84GLSAYY152 pKa = 10.18ANMRR156 pKa = 11.84NFRR159 pKa = 11.84GTTYY163 pKa = 9.87TKK165 pKa = 10.28KK166 pKa = 10.57QSIVLWGSPGCGKK179 pKa = 9.18TSTLVNYY186 pKa = 9.62FPDD189 pKa = 4.32MYY191 pKa = 11.72VKK193 pKa = 10.1MCDD196 pKa = 3.36NKK198 pKa = 9.88WWDD201 pKa = 3.84GYY203 pKa = 11.31SDD205 pKa = 3.67QKK207 pKa = 11.23QVLLDD212 pKa = 4.47DD213 pKa = 4.8FPNTYY218 pKa = 7.96TAKK221 pKa = 8.63QTKK224 pKa = 9.93DD225 pKa = 3.27LLGEE229 pKa = 4.21LDD231 pKa = 4.59LPRR234 pKa = 11.84EE235 pKa = 4.35SKK237 pKa = 10.92GQVIKK242 pKa = 10.7PRR244 pKa = 11.84VEE246 pKa = 4.04FFFITSNFHH255 pKa = 5.53PDD257 pKa = 2.63EE258 pKa = 4.28WFEE261 pKa = 4.29SAKK264 pKa = 10.18QQDD267 pKa = 3.51RR268 pKa = 11.84LAVRR272 pKa = 11.84RR273 pKa = 11.84RR274 pKa = 11.84ITKK277 pKa = 8.85TYY279 pKa = 9.51NFDD282 pKa = 3.4VEE284 pKa = 4.18EE285 pKa = 4.68EE286 pKa = 3.32IWEE289 pKa = 4.34FVRR292 pKa = 11.84EE293 pKa = 3.95FSPYY297 pKa = 9.78NVRR300 pKa = 11.84TQEE303 pKa = 3.88LL304 pKa = 3.73

Molecular weight:
35.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A346BPD5|A0A346BPD5_9VIRU Putative capsid protein OS=Sierra dome spider associated circular virus 2 OX=2293300 PE=4 SV=1
MM1 pKa = 7.16YY2 pKa = 10.36GRR4 pKa = 11.84RR5 pKa = 11.84SYY7 pKa = 10.89RR8 pKa = 11.84RR9 pKa = 11.84YY10 pKa = 7.66RR11 pKa = 11.84TYY13 pKa = 10.96RR14 pKa = 11.84KK15 pKa = 8.91GSGRR19 pKa = 11.84ARR21 pKa = 11.84YY22 pKa = 8.39GATKK26 pKa = 9.64ARR28 pKa = 11.84RR29 pKa = 11.84VVRR32 pKa = 11.84YY33 pKa = 9.06RR34 pKa = 11.84GARR37 pKa = 11.84ARR39 pKa = 11.84GIKK42 pKa = 10.09RR43 pKa = 11.84IVNRR47 pKa = 11.84QIEE50 pKa = 4.61TKK52 pKa = 9.76EE53 pKa = 4.05YY54 pKa = 10.14TNGTQTSMLQPTQLGAFGAGYY75 pKa = 10.48NGFSDD80 pKa = 3.59TVSYY84 pKa = 8.6FKK86 pKa = 11.11PMDD89 pKa = 3.82YY90 pKa = 10.73LSQGTSRR97 pKa = 11.84NTRR100 pKa = 11.84IGDD103 pKa = 3.39AVTIMSIEE111 pKa = 3.53WRR113 pKa = 11.84YY114 pKa = 10.72CITFTDD120 pKa = 4.24YY121 pKa = 9.8EE122 pKa = 4.33QEE124 pKa = 4.58FIRR127 pKa = 11.84VMAFTAPFGFTPTYY141 pKa = 11.07GNIFEE146 pKa = 5.4APDD149 pKa = 3.51GNVDD153 pKa = 3.54VRR155 pKa = 11.84VARR158 pKa = 11.84PNTNITVLKK167 pKa = 9.91QYY169 pKa = 11.0KK170 pKa = 8.39HH171 pKa = 5.58TLSPLNSATASSSSTVNTPQVFRR194 pKa = 11.84TGCLRR199 pKa = 11.84LLFPGGRR206 pKa = 11.84KK207 pKa = 8.43FQYY210 pKa = 10.67SFNNYY215 pKa = 8.42TGSTALFNRR224 pKa = 11.84NRR226 pKa = 11.84DD227 pKa = 3.2IFIVAVPFAAKK238 pKa = 10.14RR239 pKa = 11.84LQGSTLTQYY248 pKa = 10.43TFIQGQEE255 pKa = 3.91LAVKK259 pKa = 10.57FKK261 pKa = 11.03DD262 pKa = 3.33AA263 pKa = 4.38

Molecular weight:
29.89 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

567

263

304

283.5

32.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.467 ± 1.329

1.235 ± 0.295

4.586 ± 0.96

4.762 ± 1.306

5.82 ± 0.637

7.407 ± 0.596

0.882 ± 0.312

3.704 ± 0.771

5.291 ± 0.689

5.996 ± 0.655

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.175 ± 0.792

5.467 ± 0.09

4.938 ± 0.706

4.938 ± 0.003

8.466 ± 1.356

5.82 ± 0.4

8.995 ± 1.027

6.173 ± 0.292

1.235 ± 0.531

5.644 ± 0.51

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski