Amino acid dipepetide frequency for Escherichia virus Lambda_4B5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.358AlaAla: 11.358 ± 1.65
0.952AlaCys: 0.952 ± 0.287
4.557AlaAsp: 4.557 ± 0.627
6.801AlaGlu: 6.801 ± 0.825
3.673AlaPhe: 3.673 ± 0.419
7.413AlaGly: 7.413 ± 0.67
1.428AlaHis: 1.428 ± 0.269
5.985AlaIle: 5.985 ± 0.69
4.149AlaLys: 4.149 ± 0.658
8.502AlaLeu: 8.502 ± 0.679
2.585AlaMet: 2.585 ± 0.402
3.129AlaAsn: 3.129 ± 0.413
2.789AlaPro: 2.789 ± 0.443
4.285AlaGln: 4.285 ± 0.611
6.325AlaArg: 6.325 ± 0.621
6.325AlaSer: 6.325 ± 0.81
4.829AlaThr: 4.829 ± 0.919
5.849AlaVal: 5.849 ± 0.799
1.768AlaTrp: 1.768 ± 0.374
2.448AlaTyr: 2.448 ± 0.367
0.0AlaXaa: 0.0 ± 0.0
Cys
1.36CysAla: 1.36 ± 0.313
0.34CysCys: 0.34 ± 0.173
0.748CysAsp: 0.748 ± 0.248
0.68CysGlu: 0.68 ± 0.207
0.272CysPhe: 0.272 ± 0.125
1.02CysGly: 1.02 ± 0.298
0.408CysHis: 0.408 ± 0.16
0.748CysIle: 0.748 ± 0.206
0.68CysLys: 0.68 ± 0.219
1.292CysLeu: 1.292 ± 0.385
0.272CysMet: 0.272 ± 0.139
0.544CysAsn: 0.544 ± 0.163
0.272CysPro: 0.272 ± 0.127
0.408CysGln: 0.408 ± 0.155
1.156CysArg: 1.156 ± 0.292
1.02CysSer: 1.02 ± 0.269
0.884CysThr: 0.884 ± 0.26
0.748CysVal: 0.748 ± 0.22
0.272CysTrp: 0.272 ± 0.127
0.272CysTyr: 0.272 ± 0.145
0.0CysXaa: 0.0 ± 0.0
Asp
5.237AspAla: 5.237 ± 0.715
0.612AspCys: 0.612 ± 0.208
4.217AspAsp: 4.217 ± 0.52
4.081AspGlu: 4.081 ± 0.657
1.564AspPhe: 1.564 ± 0.264
4.761AspGly: 4.761 ± 0.634
0.476AspHis: 0.476 ± 0.178
3.809AspIle: 3.809 ± 0.558
2.653AspLys: 2.653 ± 0.451
3.877AspLeu: 3.877 ± 0.62
1.632AspMet: 1.632 ± 0.329
2.176AspAsn: 2.176 ± 0.393
2.38AspPro: 2.38 ± 0.504
1.428AspGln: 1.428 ± 0.359
2.721AspArg: 2.721 ± 0.39
3.333AspSer: 3.333 ± 0.62
2.925AspThr: 2.925 ± 0.506
4.081AspVal: 4.081 ± 0.53
1.156AspTrp: 1.156 ± 0.329
1.7AspTyr: 1.7 ± 0.298
0.0AspXaa: 0.0 ± 0.0
Glu
5.849GluAla: 5.849 ± 0.764
1.02GluCys: 1.02 ± 0.316
2.721GluAsp: 2.721 ± 0.418
3.673GluGlu: 3.673 ± 0.531
2.108GluPhe: 2.108 ± 0.484
3.197GluGly: 3.197 ± 0.46
1.02GluHis: 1.02 ± 0.308
3.877GluIle: 3.877 ± 0.512
3.877GluLys: 3.877 ± 0.527
5.713GluLeu: 5.713 ± 0.774
1.904GluMet: 1.904 ± 0.467
2.653GluAsn: 2.653 ± 0.369
2.244GluPro: 2.244 ± 0.33
3.945GluGln: 3.945 ± 0.741
4.761GluArg: 4.761 ± 0.653
3.401GluSer: 3.401 ± 0.418
3.333GluThr: 3.333 ± 0.47
3.061GluVal: 3.061 ± 0.401
1.292GluTrp: 1.292 ± 0.276
1.7GluTyr: 1.7 ± 0.352
0.0GluXaa: 0.0 ± 0.0
Phe
2.108PheAla: 2.108 ± 0.437
0.612PheCys: 0.612 ± 0.177
2.789PheAsp: 2.789 ± 0.416
1.904PheGlu: 1.904 ± 0.348
1.7PhePhe: 1.7 ± 0.47
2.721PheGly: 2.721 ± 0.351
0.748PheHis: 0.748 ± 0.249
1.428PheIle: 1.428 ± 0.329
1.836PheLys: 1.836 ± 0.357
2.721PheLeu: 2.721 ± 0.518
0.816PheMet: 0.816 ± 0.201
1.088PheAsn: 1.088 ± 0.243
1.836PhePro: 1.836 ± 0.428
0.68PheGln: 0.68 ± 0.217
2.789PheArg: 2.789 ± 0.373
3.469PheSer: 3.469 ± 0.552
3.401PheThr: 3.401 ± 0.435
2.925PheVal: 2.925 ± 0.4
0.612PheTrp: 0.612 ± 0.181
1.088PheTyr: 1.088 ± 0.265
0.0PheXaa: 0.0 ± 0.0
Gly
6.053GlyAla: 6.053 ± 0.845
0.816GlyCys: 0.816 ± 0.252
4.353GlyAsp: 4.353 ± 0.443
3.741GlyGlu: 3.741 ± 0.542
2.312GlyPhe: 2.312 ± 0.386
5.305GlyGly: 5.305 ± 0.865
1.088GlyHis: 1.088 ± 0.308
3.605GlyIle: 3.605 ± 0.557
4.761GlyLys: 4.761 ± 0.577
6.257GlyLeu: 6.257 ± 0.646
3.061GlyMet: 3.061 ± 0.488
3.537GlyAsn: 3.537 ± 0.388
1.224GlyPro: 1.224 ± 0.269
3.129GlyGln: 3.129 ± 0.506
3.537GlyArg: 3.537 ± 0.35
4.013GlySer: 4.013 ± 0.619
3.537GlyThr: 3.537 ± 0.524
5.237GlyVal: 5.237 ± 0.41
1.496GlyTrp: 1.496 ± 0.297
2.653GlyTyr: 2.653 ± 0.364
0.0GlyXaa: 0.0 ± 0.0
His
1.36HisAla: 1.36 ± 0.288
0.204HisCys: 0.204 ± 0.113
0.816HisAsp: 0.816 ± 0.229
0.884HisGlu: 0.884 ± 0.256
0.884HisPhe: 0.884 ± 0.314
1.36HisGly: 1.36 ± 0.299
0.204HisHis: 0.204 ± 0.118
1.224HisIle: 1.224 ± 0.298
0.816HisLys: 0.816 ± 0.235
2.04HisLeu: 2.04 ± 0.406
0.544HisMet: 0.544 ± 0.199
0.952HisAsn: 0.952 ± 0.232
0.748HisPro: 0.748 ± 0.223
0.544HisGln: 0.544 ± 0.18
1.088HisArg: 1.088 ± 0.252
0.952HisSer: 0.952 ± 0.299
0.884HisThr: 0.884 ± 0.228
0.952HisVal: 0.952 ± 0.258
0.204HisTrp: 0.204 ± 0.158
0.952HisTyr: 0.952 ± 0.243
0.0HisXaa: 0.0 ± 0.0
Ile
4.693IleAla: 4.693 ± 0.612
1.156IleCys: 1.156 ± 0.264
2.653IleAsp: 2.653 ± 0.469
3.945IleGlu: 3.945 ± 0.585
1.564IlePhe: 1.564 ± 0.29
3.333IleGly: 3.333 ± 0.477
0.68IleHis: 0.68 ± 0.204
3.537IleIle: 3.537 ± 0.547
3.333IleLys: 3.333 ± 0.564
3.605IleLeu: 3.605 ± 0.532
0.816IleMet: 0.816 ± 0.249
2.653IleAsn: 2.653 ± 0.372
2.38IlePro: 2.38 ± 0.302
2.04IleGln: 2.04 ± 0.31
2.993IleArg: 2.993 ± 0.409
4.353IleSer: 4.353 ± 0.566
4.353IleThr: 4.353 ± 0.733
3.809IleVal: 3.809 ± 0.551
0.476IleTrp: 0.476 ± 0.256
1.088IleTyr: 1.088 ± 0.312
0.0IleXaa: 0.0 ± 0.0
Lys
5.237LysAla: 5.237 ± 0.736
0.612LysCys: 0.612 ± 0.266
2.721LysAsp: 2.721 ± 0.488
3.605LysGlu: 3.605 ± 0.496
1.36LysPhe: 1.36 ± 0.263
4.217LysGly: 4.217 ± 0.589
1.088LysHis: 1.088 ± 0.265
2.04LysIle: 2.04 ± 0.435
3.741LysLys: 3.741 ± 0.45
4.149LysLeu: 4.149 ± 0.56
2.04LysMet: 2.04 ± 0.395
2.585LysAsn: 2.585 ± 0.433
2.176LysPro: 2.176 ± 0.397
2.38LysGln: 2.38 ± 0.357
3.605LysArg: 3.605 ± 0.448
2.925LysSer: 2.925 ± 0.381
3.741LysThr: 3.741 ± 0.577
3.129LysVal: 3.129 ± 0.603
1.496LysTrp: 1.496 ± 0.295
1.7LysTyr: 1.7 ± 0.326
0.0LysXaa: 0.0 ± 0.0
Leu
9.522LeuAla: 9.522 ± 0.84
1.156LeuCys: 1.156 ± 0.227
4.693LeuAsp: 4.693 ± 0.527
3.877LeuGlu: 3.877 ± 0.485
3.197LeuPhe: 3.197 ± 0.558
4.897LeuGly: 4.897 ± 0.634
1.7LeuHis: 1.7 ± 0.372
3.945LeuIle: 3.945 ± 0.556
5.985LeuLys: 5.985 ± 0.75
7.686LeuLeu: 7.686 ± 1.074
2.516LeuMet: 2.516 ± 0.537
3.605LeuAsn: 3.605 ± 0.487
3.877LeuPro: 3.877 ± 0.503
3.469LeuGln: 3.469 ± 0.459
4.965LeuArg: 4.965 ± 0.526
6.733LeuSer: 6.733 ± 0.815
5.849LeuThr: 5.849 ± 0.782
4.693LeuVal: 4.693 ± 0.624
1.836LeuTrp: 1.836 ± 0.403
1.904LeuTyr: 1.904 ± 0.395
0.0LeuXaa: 0.0 ± 0.0
Met
3.401MetAla: 3.401 ± 0.531
0.272MetCys: 0.272 ± 0.141
1.564MetAsp: 1.564 ± 0.352
0.952MetGlu: 0.952 ± 0.317
1.156MetPhe: 1.156 ± 0.263
1.496MetGly: 1.496 ± 0.349
0.68MetHis: 0.68 ± 0.21
1.496MetIle: 1.496 ± 0.399
1.7MetLys: 1.7 ± 0.335
3.197MetLeu: 3.197 ± 0.454
0.748MetMet: 0.748 ± 0.212
1.156MetAsn: 1.156 ± 0.301
1.564MetPro: 1.564 ± 0.393
1.224MetGln: 1.224 ± 0.246
1.836MetArg: 1.836 ± 0.379
1.428MetSer: 1.428 ± 0.271
2.993MetThr: 2.993 ± 0.501
2.448MetVal: 2.448 ± 0.428
0.34MetTrp: 0.34 ± 0.111
0.408MetTyr: 0.408 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
4.149AsnAla: 4.149 ± 0.637
0.748AsnCys: 0.748 ± 0.23
1.836AsnAsp: 1.836 ± 0.296
2.653AsnGlu: 2.653 ± 0.404
1.496AsnPhe: 1.496 ± 0.371
3.265AsnGly: 3.265 ± 0.565
0.884AsnHis: 0.884 ± 0.25
2.176AsnIle: 2.176 ± 0.415
2.244AsnLys: 2.244 ± 0.465
2.516AsnLeu: 2.516 ± 0.403
1.088AsnMet: 1.088 ± 0.275
2.108AsnAsn: 2.108 ± 0.438
1.972AsnPro: 1.972 ± 0.345
1.292AsnGln: 1.292 ± 0.295
2.857AsnArg: 2.857 ± 0.638
2.176AsnSer: 2.176 ± 0.413
2.653AsnThr: 2.653 ± 0.348
2.448AsnVal: 2.448 ± 0.351
0.476AsnTrp: 0.476 ± 0.171
1.496AsnTyr: 1.496 ± 0.327
0.0AsnXaa: 0.0 ± 0.0
Pro
3.333ProAla: 3.333 ± 0.537
0.272ProCys: 0.272 ± 0.125
3.537ProAsp: 3.537 ± 0.511
2.857ProGlu: 2.857 ± 0.497
1.632ProPhe: 1.632 ± 0.339
3.197ProGly: 3.197 ± 0.428
0.884ProHis: 0.884 ± 0.25
1.428ProIle: 1.428 ± 0.3
1.564ProLys: 1.564 ± 0.338
2.653ProLeu: 2.653 ± 0.459
0.476ProMet: 0.476 ± 0.156
1.564ProAsn: 1.564 ± 0.3
1.36ProPro: 1.36 ± 0.334
1.564ProGln: 1.564 ± 0.274
1.972ProArg: 1.972 ± 0.331
2.448ProSer: 2.448 ± 0.459
2.516ProThr: 2.516 ± 0.438
3.469ProVal: 3.469 ± 0.501
0.748ProTrp: 0.748 ± 0.263
0.748ProTyr: 0.748 ± 0.228
0.0ProXaa: 0.0 ± 0.0
Gln
4.149GlnAla: 4.149 ± 0.763
0.544GlnCys: 0.544 ± 0.2
1.292GlnAsp: 1.292 ± 0.306
2.993GlnGlu: 2.993 ± 0.495
1.224GlnPhe: 1.224 ± 0.302
2.448GlnGly: 2.448 ± 0.316
0.68GlnHis: 0.68 ± 0.269
2.653GlnIle: 2.653 ± 0.465
1.972GlnLys: 1.972 ± 0.343
3.605GlnLeu: 3.605 ± 0.405
1.496GlnMet: 1.496 ± 0.319
2.04GlnAsn: 2.04 ± 0.356
1.224GlnPro: 1.224 ± 0.252
2.516GlnGln: 2.516 ± 0.432
3.197GlnArg: 3.197 ± 0.44
2.925GlnSer: 2.925 ± 0.446
2.585GlnThr: 2.585 ± 0.413
2.993GlnVal: 2.993 ± 0.567
0.544GlnTrp: 0.544 ± 0.211
1.088GlnTyr: 1.088 ± 0.241
0.0GlnXaa: 0.0 ± 0.0
Arg
4.693ArgAla: 4.693 ± 0.589
0.612ArgCys: 0.612 ± 0.199
3.469ArgAsp: 3.469 ± 0.641
4.625ArgGlu: 4.625 ± 0.569
2.789ArgPhe: 2.789 ± 0.462
3.469ArgGly: 3.469 ± 0.582
1.564ArgHis: 1.564 ± 0.322
3.605ArgIle: 3.605 ± 0.569
3.741ArgLys: 3.741 ± 0.576
6.121ArgLeu: 6.121 ± 0.784
2.721ArgMet: 2.721 ± 0.393
2.721ArgAsn: 2.721 ± 0.543
1.904ArgPro: 1.904 ± 0.331
3.673ArgGln: 3.673 ± 0.464
5.713ArgArg: 5.713 ± 0.889
2.653ArgSer: 2.653 ± 0.437
2.721ArgThr: 2.721 ± 0.386
3.469ArgVal: 3.469 ± 0.502
1.224ArgTrp: 1.224 ± 0.306
2.04ArgTyr: 2.04 ± 0.312
0.0ArgXaa: 0.0 ± 0.0
Ser
6.461SerAla: 6.461 ± 0.717
0.816SerCys: 0.816 ± 0.304
3.401SerAsp: 3.401 ± 0.484
4.557SerGlu: 4.557 ± 0.536
2.516SerPhe: 2.516 ± 0.428
6.257SerGly: 6.257 ± 0.731
1.36SerHis: 1.36 ± 0.283
3.333SerIle: 3.333 ± 0.45
2.516SerLys: 2.516 ± 0.404
5.033SerLeu: 5.033 ± 0.579
2.312SerMet: 2.312 ± 0.362
1.496SerAsn: 1.496 ± 0.322
2.516SerPro: 2.516 ± 0.395
2.857SerGln: 2.857 ± 0.393
4.353SerArg: 4.353 ± 0.548
3.129SerSer: 3.129 ± 0.443
3.537SerThr: 3.537 ± 0.543
5.509SerVal: 5.509 ± 0.547
0.884SerTrp: 0.884 ± 0.24
2.04SerTyr: 2.04 ± 0.419
0.0SerXaa: 0.0 ± 0.0
Thr
6.665ThrAla: 6.665 ± 0.795
0.68ThrCys: 0.68 ± 0.218
3.401ThrAsp: 3.401 ± 0.368
4.081ThrGlu: 4.081 ± 0.464
2.789ThrPhe: 2.789 ± 0.377
4.625ThrGly: 4.625 ± 0.584
1.088ThrHis: 1.088 ± 0.244
2.857ThrIle: 2.857 ± 0.51
2.653ThrLys: 2.653 ± 0.489
6.461ThrLeu: 6.461 ± 0.619
1.496ThrMet: 1.496 ± 0.372
1.7ThrAsn: 1.7 ± 0.43
3.537ThrPro: 3.537 ± 0.599
2.108ThrGln: 2.108 ± 0.435
3.061ThrArg: 3.061 ± 0.41
4.217ThrSer: 4.217 ± 0.476
3.469ThrThr: 3.469 ± 0.657
4.693ThrVal: 4.693 ± 0.878
0.884ThrTrp: 0.884 ± 0.305
2.176ThrTyr: 2.176 ± 0.451
0.0ThrXaa: 0.0 ± 0.0
Val
5.645ValAla: 5.645 ± 0.607
1.02ValCys: 1.02 ± 0.317
3.537ValAsp: 3.537 ± 0.509
3.061ValGlu: 3.061 ± 0.395
2.993ValPhe: 2.993 ± 0.415
3.741ValGly: 3.741 ± 0.503
0.68ValHis: 0.68 ± 0.181
3.265ValIle: 3.265 ± 0.455
4.353ValLys: 4.353 ± 0.602
5.713ValLeu: 5.713 ± 0.704
2.04ValMet: 2.04 ± 0.384
3.673ValAsn: 3.673 ± 0.447
2.244ValPro: 2.244 ± 0.46
2.312ValGln: 2.312 ± 0.545
3.333ValArg: 3.333 ± 0.44
5.577ValSer: 5.577 ± 0.774
5.645ValThr: 5.645 ± 0.673
5.441ValVal: 5.441 ± 0.521
1.292ValTrp: 1.292 ± 0.302
2.04ValTyr: 2.04 ± 0.411
0.0ValXaa: 0.0 ± 0.0
Trp
1.496TrpAla: 1.496 ± 0.286
0.544TrpCys: 0.544 ± 0.198
0.884TrpAsp: 0.884 ± 0.298
0.748TrpGlu: 0.748 ± 0.212
0.544TrpPhe: 0.544 ± 0.164
0.952TrpGly: 0.952 ± 0.204
0.476TrpHis: 0.476 ± 0.175
1.02TrpIle: 1.02 ± 0.274
0.748TrpLys: 0.748 ± 0.237
2.04TrpLeu: 2.04 ± 0.409
0.816TrpMet: 0.816 ± 0.193
0.34TrpAsn: 0.34 ± 0.136
0.816TrpPro: 0.816 ± 0.238
0.748TrpGln: 0.748 ± 0.222
1.088TrpArg: 1.088 ± 0.293
1.156TrpSer: 1.156 ± 0.249
1.224TrpThr: 1.224 ± 0.329
1.224TrpVal: 1.224 ± 0.318
0.272TrpTrp: 0.272 ± 0.164
0.68TrpTyr: 0.68 ± 0.167
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.448TyrAla: 2.448 ± 0.399
0.408TyrCys: 0.408 ± 0.166
1.632TyrAsp: 1.632 ± 0.232
1.632TyrGlu: 1.632 ± 0.287
1.496TyrPhe: 1.496 ± 0.357
1.972TyrGly: 1.972 ± 0.346
0.476TyrHis: 0.476 ± 0.173
1.428TyrIle: 1.428 ± 0.33
1.224TyrLys: 1.224 ± 0.308
2.925TyrLeu: 2.925 ± 0.469
0.68TyrMet: 0.68 ± 0.283
0.816TyrAsn: 0.816 ± 0.242
1.156TyrPro: 1.156 ± 0.293
1.564TyrGln: 1.564 ± 0.292
2.176TyrArg: 2.176 ± 0.422
2.653TyrSer: 2.653 ± 0.459
1.564TyrThr: 1.564 ± 0.312
1.428TyrVal: 1.428 ± 0.31
0.544TyrTrp: 0.544 ± 0.21
0.884TyrTyr: 0.884 ± 0.219
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 75 proteins (14704 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski