Escherichia phage Evi

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A653FX14|A0A653FX14_9CAUD Primosomal protein I OS=Escherichia phage Evi OX=2562642 PE=4 SV=1
MM1 pKa = 7.58PEE3 pKa = 4.75EE4 pKa = 4.09YY5 pKa = 10.19VQPYY9 pKa = 9.57SVLVEE14 pKa = 4.04CHH16 pKa = 6.36ALQDD20 pKa = 3.98AAPDD24 pKa = 4.09AEE26 pKa = 4.16PHH28 pKa = 4.95QAEE31 pKa = 4.31VLIVHH36 pKa = 6.55FVPAALAQASS46 pKa = 3.68

Molecular weight: 4.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A653FU31|A0A653FU31_9CAUD Phage head maturation protease OS=Escherichia phage Evi OX=2562642 PE=4 SV=1
MM1 pKa = 6.78QAGRR5 pKa = 11.84LRR7 pKa = 11.84DD8 pKa = 3.35RR9 pKa = 11.84VIILNVTTARR19 pKa = 11.84SPSGHH24 pKa = 6.33PVEE27 pKa = 4.87TVTEE31 pKa = 4.65GATVWAEE38 pKa = 3.78VKK40 pKa = 10.5GISGRR45 pKa = 11.84EE46 pKa = 4.0IISGGAEE53 pKa = 3.65TAQATVRR60 pKa = 11.84VWMRR64 pKa = 11.84FRR66 pKa = 11.84RR67 pKa = 11.84DD68 pKa = 2.89VTATSRR74 pKa = 11.84LKK76 pKa = 11.07VLTGAFKK83 pKa = 10.84GAILGIEE90 pKa = 4.49GPPIPDD96 pKa = 3.01ARR98 pKa = 11.84ATRR101 pKa = 11.84LEE103 pKa = 4.16ILCSLKK109 pKa = 11.17GNVV112 pKa = 3.5

Molecular weight: 11.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

11560

40

1137

199.3

22.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.0 ± 0.587

1.228 ± 0.189

5.545 ± 0.256

6.92 ± 0.355

3.14 ± 0.223

7.076 ± 0.408

1.799 ± 0.272

5.13 ± 0.284

5.355 ± 0.262

7.958 ± 0.374

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.517 ± 0.146

4.239 ± 0.273

4.17 ± 0.414

4.619 ± 0.328

6.462 ± 0.298

6.445 ± 0.375

6.047 ± 0.356

6.912 ± 0.292

1.661 ± 0.127

2.777 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski