[Ruminococcus] gnavus CAG:126

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Ruminococcus; environmental samples

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2588 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5TX50|R5TX50_9FIRM Uncharacterized protein OS=[Ruminococcus] gnavus CAG:126 OX=1263106 GN=BN481_00740 PE=4 SV=1
MM1 pKa = 7.53EE2 pKa = 4.77EE3 pKa = 4.48EE4 pKa = 4.43IVLCAANSYY13 pKa = 6.89EE14 pKa = 3.89QKK16 pKa = 10.96YY17 pKa = 9.01YY18 pKa = 11.04LNPEE22 pKa = 4.1FEE24 pKa = 4.78MLPEE28 pKa = 4.66SIKK31 pKa = 10.59QEE33 pKa = 4.06LNIMCVLYY41 pKa = 10.46TEE43 pKa = 4.72DD44 pKa = 3.03VGGILMVFYY53 pKa = 11.01DD54 pKa = 4.11EE55 pKa = 5.9DD56 pKa = 4.43GNLEE60 pKa = 3.75FRR62 pKa = 11.84VDD64 pKa = 3.35HH65 pKa = 6.71EE66 pKa = 4.82EE67 pKa = 3.84NDD69 pKa = 3.47FSFDD73 pKa = 3.81EE74 pKa = 4.52IGSVLKK80 pKa = 10.45IKK82 pKa = 10.26QLQQTKK88 pKa = 9.98QEE90 pKa = 4.16LFEE93 pKa = 4.29SLEE96 pKa = 3.89LFYY99 pKa = 11.2KK100 pKa = 10.31VFYY103 pKa = 10.69LGEE106 pKa = 4.16EE107 pKa = 3.9VDD109 pKa = 4.78LEE111 pKa = 4.41EE112 pKa = 4.62EE113 pKa = 4.17

Molecular weight: 13.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5TLW1|R5TLW1_9FIRM Uncharacterized protein OS=[Ruminococcus] gnavus CAG:126 OX=1263106 GN=BN481_01936 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 9.27VHH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.18GRR39 pKa = 11.84KK40 pKa = 8.18QLSAA44 pKa = 3.9

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2588

0

2588

793556

29

2177

306.6

34.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.412 ± 0.05

1.509 ± 0.02

5.203 ± 0.039

8.29 ± 0.057

4.058 ± 0.034

7.127 ± 0.039

1.793 ± 0.023

7.284 ± 0.04

6.98 ± 0.041

9.07 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.248 ± 0.021

3.994 ± 0.03

3.256 ± 0.026

3.603 ± 0.026

4.417 ± 0.039

5.483 ± 0.03

5.281 ± 0.03

7.075 ± 0.045

0.912 ± 0.018

4.003 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski