Tissierella praeacuta DSM 18095

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Tissierellaceae; Tissierella; Tissierella praeacuta

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3095 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M4V032|A0A1M4V032_9FIRM Probable cell division protein WhiA OS=Tissierella praeacuta DSM 18095 OX=1123404 GN=whiA PE=3 SV=1
MM1 pKa = 7.73EE2 pKa = 5.93IIKK5 pKa = 10.31EE6 pKa = 4.06NSNSSIIIPIEE17 pKa = 3.78VLKK20 pKa = 11.08VSNLLDD26 pKa = 3.86SKK28 pKa = 11.16DD29 pKa = 3.66IEE31 pKa = 4.85LKK33 pKa = 9.03TLEE36 pKa = 4.11NAFVVMKK43 pKa = 9.81GTMNAMEE50 pKa = 5.4LIKK53 pKa = 10.62LAEE56 pKa = 4.17GLKK59 pKa = 10.41NLSEE63 pKa = 4.19EE64 pKa = 4.67LIEE67 pKa = 4.91HH68 pKa = 6.84LAGVCGRR75 pKa = 11.84CYY77 pKa = 10.6DD78 pKa = 3.39CSYY81 pKa = 11.1CEE83 pKa = 3.93RR84 pKa = 11.84FEE86 pKa = 4.54EE87 pKa = 4.23YY88 pKa = 10.91DD89 pKa = 3.79EE90 pKa = 4.98IVVPDD95 pKa = 4.0YY96 pKa = 11.18LLEE99 pKa = 4.24EE100 pKa = 4.37AGIPIDD106 pKa = 5.09AKK108 pKa = 9.95LCAYY112 pKa = 9.28TEE114 pKa = 4.14EE115 pKa = 4.84DD116 pKa = 3.15SGEE119 pKa = 4.28VVVIQADD126 pKa = 3.74YY127 pKa = 10.79DD128 pKa = 3.96YY129 pKa = 10.92DD130 pKa = 3.75IADD133 pKa = 3.55VPQFVIDD140 pKa = 3.39IFEE143 pKa = 4.06ISGICIRR150 pKa = 11.84EE151 pKa = 4.18LEE153 pKa = 4.41EE154 pKa = 3.74NLIMEE159 pKa = 4.94NIVYY163 pKa = 10.46GDD165 pKa = 3.53EE166 pKa = 4.04

Molecular weight: 18.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M4SDJ3|A0A1M4SDJ3_9FIRM Geranylgeranyl diphosphate synthase type II OS=Tissierella praeacuta DSM 18095 OX=1123404 GN=SAMN02745784_00264 PE=3 SV=1
MM1 pKa = 7.67KK2 pKa = 10.0KK3 pKa = 10.47NKK5 pKa = 9.64IMTLGLSIALIIAMAGCTPKK25 pKa = 9.65GTNMRR30 pKa = 11.84NMSTQTRR37 pKa = 11.84LYY39 pKa = 10.66DD40 pKa = 3.53NNVNNRR46 pKa = 11.84WMTNAPFNARR56 pKa = 11.84DD57 pKa = 3.64RR58 pKa = 11.84LNTNLNNGMVRR69 pKa = 11.84NNSYY73 pKa = 11.32LNDD76 pKa = 3.45GVLRR80 pKa = 11.84DD81 pKa = 4.11NNYY84 pKa = 10.53LNNNVVRR91 pKa = 11.84DD92 pKa = 3.88NTLTNDD98 pKa = 3.13NIMRR102 pKa = 11.84NRR104 pKa = 11.84TNLDD108 pKa = 2.72SGMITNDD115 pKa = 2.4ISQLSTRR122 pKa = 11.84ADD124 pKa = 3.85AIARR128 pKa = 11.84RR129 pKa = 11.84VTALPEE135 pKa = 3.77VTGASVIVHH144 pKa = 6.67GNTAIVGCDD153 pKa = 3.15VRR155 pKa = 11.84GNTNNAISSNLKK167 pKa = 9.87QKK169 pKa = 10.72VEE171 pKa = 4.16AAVKK175 pKa = 8.81TADD178 pKa = 3.8RR179 pKa = 11.84NIKK182 pKa = 9.54KK183 pKa = 10.47VSVTSDD189 pKa = 3.03PSLYY193 pKa = 10.68SRR195 pKa = 11.84IRR197 pKa = 11.84TMSTSIGNGHH207 pKa = 7.62PISNFTRR214 pKa = 11.84DD215 pKa = 2.83IEE217 pKa = 5.04DD218 pKa = 2.96ILRR221 pKa = 11.84RR222 pKa = 11.84ITSPIRR228 pKa = 3.45

Molecular weight: 25.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3095

0

3095

911760

39

3743

294.6

33.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.332 ± 0.057

0.891 ± 0.018

5.57 ± 0.038

7.834 ± 0.057

4.312 ± 0.036

6.675 ± 0.044

1.387 ± 0.017

10.669 ± 0.06

8.649 ± 0.046

9.25 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.71 ± 0.021

6.046 ± 0.043

2.935 ± 0.026

2.313 ± 0.024

3.662 ± 0.031

6.021 ± 0.033

4.877 ± 0.029

6.127 ± 0.036

0.679 ± 0.013

4.06 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski