Amino acid dipepetide frequency for Rhodovulum phage vB_RhkS_P1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
22.757AlaAla: 22.757 ± 2.154
1.068AlaCys: 1.068 ± 0.343
10.105AlaAsp: 10.105 ± 0.775
10.516AlaGlu: 10.516 ± 0.993
3.943AlaPhe: 3.943 ± 0.421
11.584AlaGly: 11.584 ± 1.092
2.136AlaHis: 2.136 ± 0.517
5.258AlaIle: 5.258 ± 0.707
4.601AlaLys: 4.601 ± 0.677
13.884AlaLeu: 13.884 ± 1.441
3.533AlaMet: 3.533 ± 0.526
2.383AlaAsn: 2.383 ± 0.453
7.394AlaPro: 7.394 ± 0.895
4.436AlaGln: 4.436 ± 0.897
12.488AlaArg: 12.488 ± 1.511
7.312AlaSer: 7.312 ± 1.048
7.312AlaThr: 7.312 ± 1.033
8.216AlaVal: 8.216 ± 0.852
2.547AlaTrp: 2.547 ± 0.429
2.465AlaTyr: 2.465 ± 0.531
0.0AlaXaa: 0.0 ± 0.0
Cys
0.986CysAla: 0.986 ± 0.335
0.082CysCys: 0.082 ± 0.093
0.575CysAsp: 0.575 ± 0.213
0.246CysGlu: 0.246 ± 0.149
0.329CysPhe: 0.329 ± 0.186
0.493CysGly: 0.493 ± 0.244
0.329CysHis: 0.329 ± 0.166
0.329CysIle: 0.329 ± 0.141
0.0CysLys: 0.0 ± 0.0
1.232CysLeu: 1.232 ± 0.261
0.082CysMet: 0.082 ± 0.076
0.164CysAsn: 0.164 ± 0.103
0.739CysPro: 0.739 ± 0.285
0.246CysGln: 0.246 ± 0.126
0.493CysArg: 0.493 ± 0.185
0.493CysSer: 0.493 ± 0.228
0.329CysThr: 0.329 ± 0.132
0.493CysVal: 0.493 ± 0.22
0.164CysTrp: 0.164 ± 0.097
0.329CysTyr: 0.329 ± 0.153
0.0CysXaa: 0.0 ± 0.0
Asp
8.873AspAla: 8.873 ± 1.027
0.657AspCys: 0.657 ± 0.192
5.833AspAsp: 5.833 ± 0.785
4.19AspGlu: 4.19 ± 0.401
2.054AspPhe: 2.054 ± 0.468
6.244AspGly: 6.244 ± 0.785
1.15AspHis: 1.15 ± 0.324
4.19AspIle: 4.19 ± 0.549
0.575AspLys: 0.575 ± 0.227
7.148AspLeu: 7.148 ± 0.865
1.89AspMet: 1.89 ± 0.419
0.986AspAsn: 0.986 ± 0.273
4.519AspPro: 4.519 ± 0.51
2.218AspGln: 2.218 ± 0.329
5.176AspArg: 5.176 ± 0.691
2.054AspSer: 2.054 ± 0.365
3.451AspThr: 3.451 ± 0.548
5.422AspVal: 5.422 ± 0.618
2.136AspTrp: 2.136 ± 0.417
2.218AspTyr: 2.218 ± 0.54
0.0AspXaa: 0.0 ± 0.0
Glu
8.709GluAla: 8.709 ± 1.051
0.575GluCys: 0.575 ± 0.276
4.026GluAsp: 4.026 ± 0.632
3.861GluGlu: 3.861 ± 0.709
1.807GluPhe: 1.807 ± 0.379
4.354GluGly: 4.354 ± 0.729
1.15GluHis: 1.15 ± 0.36
5.176GluIle: 5.176 ± 0.682
1.643GluLys: 1.643 ± 0.369
5.176GluLeu: 5.176 ± 0.584
2.054GluMet: 2.054 ± 0.375
1.232GluAsn: 1.232 ± 0.345
2.547GluPro: 2.547 ± 0.458
2.054GluGln: 2.054 ± 0.469
4.847GluArg: 4.847 ± 0.724
0.739GluSer: 0.739 ± 0.259
4.19GluThr: 4.19 ± 0.616
4.519GluVal: 4.519 ± 0.608
0.739GluTrp: 0.739 ± 0.228
0.575GluTyr: 0.575 ± 0.18
0.0GluXaa: 0.0 ± 0.0
Phe
4.272PheAla: 4.272 ± 0.472
0.411PheCys: 0.411 ± 0.151
2.547PheAsp: 2.547 ± 0.516
1.89PheGlu: 1.89 ± 0.426
0.657PhePhe: 0.657 ± 0.31
3.04PheGly: 3.04 ± 0.548
0.411PheHis: 0.411 ± 0.191
0.411PheIle: 0.411 ± 0.162
0.739PheLys: 0.739 ± 0.228
1.643PheLeu: 1.643 ± 0.369
0.329PheMet: 0.329 ± 0.144
0.657PheAsn: 0.657 ± 0.296
1.15PhePro: 1.15 ± 0.287
1.314PheGln: 1.314 ± 0.339
2.3PheArg: 2.3 ± 0.462
1.807PheSer: 1.807 ± 0.498
1.725PheThr: 1.725 ± 0.469
2.383PheVal: 2.383 ± 0.363
0.493PheTrp: 0.493 ± 0.229
0.411PheTyr: 0.411 ± 0.132
0.0PheXaa: 0.0 ± 0.0
Gly
10.269GlyAla: 10.269 ± 0.974
0.657GlyCys: 0.657 ± 0.306
5.176GlyAsp: 5.176 ± 0.677
5.258GlyGlu: 5.258 ± 0.595
2.711GlyPhe: 2.711 ± 0.539
7.805GlyGly: 7.805 ± 0.794
2.465GlyHis: 2.465 ± 0.652
2.875GlyIle: 2.875 ± 0.552
2.3GlyLys: 2.3 ± 0.469
6.901GlyLeu: 6.901 ± 0.638
1.972GlyMet: 1.972 ± 0.478
1.972GlyAsn: 1.972 ± 0.676
3.451GlyPro: 3.451 ± 0.456
3.697GlyGln: 3.697 ± 0.447
5.833GlyArg: 5.833 ± 0.632
4.108GlySer: 4.108 ± 0.692
5.258GlyThr: 5.258 ± 1.0
5.833GlyVal: 5.833 ± 0.606
2.547GlyTrp: 2.547 ± 0.45
1.561GlyTyr: 1.561 ± 0.396
0.0GlyXaa: 0.0 ± 0.0
His
3.04HisAla: 3.04 ± 0.594
0.493HisCys: 0.493 ± 0.213
1.397HisAsp: 1.397 ± 0.297
0.822HisGlu: 0.822 ± 0.273
0.411HisPhe: 0.411 ± 0.178
1.314HisGly: 1.314 ± 0.278
0.575HisHis: 0.575 ± 0.244
1.232HisIle: 1.232 ± 0.435
0.246HisLys: 0.246 ± 0.14
1.561HisLeu: 1.561 ± 0.487
0.329HisMet: 0.329 ± 0.152
0.575HisAsn: 0.575 ± 0.229
1.314HisPro: 1.314 ± 0.29
0.329HisGln: 0.329 ± 0.16
1.314HisArg: 1.314 ± 0.3
0.986HisSer: 0.986 ± 0.247
0.739HisThr: 0.739 ± 0.312
1.643HisVal: 1.643 ± 0.392
0.411HisTrp: 0.411 ± 0.198
0.575HisTyr: 0.575 ± 0.202
0.0HisXaa: 0.0 ± 0.0
Ile
5.915IleAla: 5.915 ± 0.925
0.329IleCys: 0.329 ± 0.149
4.354IleAsp: 4.354 ± 0.514
3.861IleGlu: 3.861 ± 0.466
0.822IlePhe: 0.822 ± 0.192
3.615IleGly: 3.615 ± 0.651
1.479IleHis: 1.479 ± 0.266
0.904IleIle: 0.904 ± 0.231
0.904IleLys: 0.904 ± 0.279
3.451IleLeu: 3.451 ± 0.472
0.575IleMet: 0.575 ± 0.226
1.15IleAsn: 1.15 ± 0.312
3.122IlePro: 3.122 ± 0.597
1.479IleGln: 1.479 ± 0.378
3.697IleArg: 3.697 ± 0.539
1.89IleSer: 1.89 ± 0.403
2.875IleThr: 2.875 ± 0.643
2.465IleVal: 2.465 ± 0.462
0.986IleTrp: 0.986 ± 0.292
1.314IleTyr: 1.314 ± 0.373
0.0IleXaa: 0.0 ± 0.0
Lys
3.779LysAla: 3.779 ± 0.6
0.082LysCys: 0.082 ± 0.085
1.561LysAsp: 1.561 ± 0.369
0.575LysGlu: 0.575 ± 0.245
0.575LysPhe: 0.575 ± 0.236
2.465LysGly: 2.465 ± 0.659
0.164LysHis: 0.164 ± 0.133
1.89LysIle: 1.89 ± 0.469
0.657LysLys: 0.657 ± 0.31
1.89LysLeu: 1.89 ± 0.402
0.411LysMet: 0.411 ± 0.177
0.411LysAsn: 0.411 ± 0.185
1.725LysPro: 1.725 ± 0.477
0.739LysGln: 0.739 ± 0.312
2.136LysArg: 2.136 ± 0.477
0.904LysSer: 0.904 ± 0.273
2.3LysThr: 2.3 ± 0.473
1.725LysVal: 1.725 ± 0.394
0.411LysTrp: 0.411 ± 0.178
0.575LysTyr: 0.575 ± 0.197
0.0LysXaa: 0.0 ± 0.0
Leu
12.077LeuAla: 12.077 ± 1.013
0.493LeuCys: 0.493 ± 0.213
6.737LeuAsp: 6.737 ± 0.499
5.34LeuGlu: 5.34 ± 0.886
2.547LeuPhe: 2.547 ± 0.555
7.394LeuGly: 7.394 ± 0.797
1.479LeuHis: 1.479 ± 0.348
3.533LeuIle: 3.533 ± 0.521
2.875LeuLys: 2.875 ± 0.429
5.176LeuLeu: 5.176 ± 0.607
1.643LeuMet: 1.643 ± 0.454
1.479LeuAsn: 1.479 ± 0.36
4.519LeuPro: 4.519 ± 0.748
3.533LeuGln: 3.533 ± 0.586
8.38LeuArg: 8.38 ± 1.154
5.915LeuSer: 5.915 ± 0.8
5.997LeuThr: 5.997 ± 0.726
5.012LeuVal: 5.012 ± 0.596
1.314LeuTrp: 1.314 ± 0.277
2.136LeuTyr: 2.136 ± 0.45
0.0LeuXaa: 0.0 ± 0.0
Met
3.204MetAla: 3.204 ± 0.655
0.0MetCys: 0.0 ± 0.0
1.479MetAsp: 1.479 ± 0.354
1.232MetGlu: 1.232 ± 0.21
0.739MetPhe: 0.739 ± 0.241
2.136MetGly: 2.136 ± 0.375
0.575MetHis: 0.575 ± 0.205
1.314MetIle: 1.314 ± 0.327
0.411MetLys: 0.411 ± 0.163
1.397MetLeu: 1.397 ± 0.367
0.411MetMet: 0.411 ± 0.18
0.329MetAsn: 0.329 ± 0.158
1.725MetPro: 1.725 ± 0.397
0.822MetGln: 0.822 ± 0.258
2.218MetArg: 2.218 ± 0.415
1.725MetSer: 1.725 ± 0.414
2.465MetThr: 2.465 ± 0.408
1.314MetVal: 1.314 ± 0.284
0.329MetTrp: 0.329 ± 0.128
0.082MetTyr: 0.082 ± 0.099
0.0MetXaa: 0.0 ± 0.0
Asn
3.04AsnAla: 3.04 ± 0.573
0.329AsnCys: 0.329 ± 0.134
0.822AsnAsp: 0.822 ± 0.244
0.329AsnGlu: 0.329 ± 0.145
0.411AsnPhe: 0.411 ± 0.2
2.218AsnGly: 2.218 ± 0.271
0.329AsnHis: 0.329 ± 0.204
1.068AsnIle: 1.068 ± 0.263
0.739AsnLys: 0.739 ± 0.256
1.807AsnLeu: 1.807 ± 0.365
0.575AsnMet: 0.575 ± 0.236
0.411AsnAsn: 0.411 ± 0.154
1.15AsnPro: 1.15 ± 0.31
0.164AsnGln: 0.164 ± 0.122
1.561AsnArg: 1.561 ± 0.329
0.822AsnSer: 0.822 ± 0.217
1.397AsnThr: 1.397 ± 0.419
1.561AsnVal: 1.561 ± 0.381
0.246AsnTrp: 0.246 ± 0.111
0.411AsnTyr: 0.411 ± 0.171
0.0AsnXaa: 0.0 ± 0.0
Pro
9.448ProAla: 9.448 ± 0.998
0.493ProCys: 0.493 ± 0.202
4.519ProAsp: 4.519 ± 0.601
3.451ProGlu: 3.451 ± 0.493
1.15ProPhe: 1.15 ± 0.306
4.354ProGly: 4.354 ± 0.659
1.232ProHis: 1.232 ± 0.382
1.561ProIle: 1.561 ± 0.36
1.314ProLys: 1.314 ± 0.328
4.19ProLeu: 4.19 ± 0.41
1.314ProMet: 1.314 ± 0.371
1.232ProAsn: 1.232 ± 0.323
2.958ProPro: 2.958 ± 0.549
1.479ProGln: 1.479 ± 0.323
4.026ProArg: 4.026 ± 0.548
3.122ProSer: 3.122 ± 0.555
2.218ProThr: 2.218 ± 0.647
4.026ProVal: 4.026 ± 0.551
0.657ProTrp: 0.657 ± 0.278
0.986ProTyr: 0.986 ± 0.267
0.0ProXaa: 0.0 ± 0.0
Gln
4.519GlnAla: 4.519 ± 0.754
0.082GlnCys: 0.082 ± 0.069
2.547GlnAsp: 2.547 ± 0.506
1.643GlnGlu: 1.643 ± 0.337
0.575GlnPhe: 0.575 ± 0.203
1.725GlnGly: 1.725 ± 0.397
0.082GlnHis: 0.082 ± 0.079
2.136GlnIle: 2.136 ± 0.382
1.068GlnLys: 1.068 ± 0.368
2.711GlnLeu: 2.711 ± 0.555
1.232GlnMet: 1.232 ± 0.346
0.329GlnAsn: 0.329 ± 0.156
1.314GlnPro: 1.314 ± 0.347
0.739GlnGln: 0.739 ± 0.258
2.547GlnArg: 2.547 ± 0.446
1.89GlnSer: 1.89 ± 0.548
2.875GlnThr: 2.875 ± 0.461
3.368GlnVal: 3.368 ± 0.546
0.657GlnTrp: 0.657 ± 0.225
0.657GlnTyr: 0.657 ± 0.206
0.0GlnXaa: 0.0 ± 0.0
Arg
10.762ArgAla: 10.762 ± 1.454
0.493ArgCys: 0.493 ± 0.192
5.012ArgAsp: 5.012 ± 0.619
3.943ArgGlu: 3.943 ± 0.659
3.533ArgPhe: 3.533 ± 0.54
5.012ArgGly: 5.012 ± 0.597
2.136ArgHis: 2.136 ± 0.426
4.026ArgIle: 4.026 ± 0.491
2.711ArgLys: 2.711 ± 0.574
9.037ArgLeu: 9.037 ± 0.895
2.218ArgMet: 2.218 ± 0.392
1.314ArgAsn: 1.314 ± 0.297
4.765ArgPro: 4.765 ± 0.704
3.533ArgGln: 3.533 ± 0.614
6.572ArgArg: 6.572 ± 1.352
3.204ArgSer: 3.204 ± 0.55
3.122ArgThr: 3.122 ± 0.663
5.751ArgVal: 5.751 ± 0.751
1.972ArgTrp: 1.972 ± 0.358
1.807ArgTyr: 1.807 ± 0.337
0.0ArgXaa: 0.0 ± 0.0
Ser
7.558SerAla: 7.558 ± 1.413
0.329SerCys: 0.329 ± 0.201
3.533SerAsp: 3.533 ± 0.663
3.204SerGlu: 3.204 ± 0.563
1.643SerPhe: 1.643 ± 0.439
5.422SerGly: 5.422 ± 0.917
0.329SerHis: 0.329 ± 0.138
2.465SerIle: 2.465 ± 0.504
1.232SerLys: 1.232 ± 0.327
4.19SerLeu: 4.19 ± 0.622
1.972SerMet: 1.972 ± 0.307
0.904SerAsn: 0.904 ± 0.255
1.725SerPro: 1.725 ± 0.376
0.904SerGln: 0.904 ± 0.26
3.779SerArg: 3.779 ± 0.825
2.3SerSer: 2.3 ± 0.411
1.643SerThr: 1.643 ± 0.323
3.533SerVal: 3.533 ± 1.006
0.904SerTrp: 0.904 ± 0.213
0.986SerTyr: 0.986 ± 0.301
0.0SerXaa: 0.0 ± 0.0
Thr
9.119ThrAla: 9.119 ± 0.939
0.411ThrCys: 0.411 ± 0.17
4.19ThrAsp: 4.19 ± 0.659
3.04ThrGlu: 3.04 ± 0.538
1.397ThrPhe: 1.397 ± 0.305
6.162ThrGly: 6.162 ± 0.73
1.068ThrHis: 1.068 ± 0.301
2.958ThrIle: 2.958 ± 0.609
0.986ThrLys: 0.986 ± 0.289
5.587ThrLeu: 5.587 ± 0.723
1.15ThrMet: 1.15 ± 0.269
1.397ThrAsn: 1.397 ± 0.534
4.272ThrPro: 4.272 ± 0.515
1.068ThrGln: 1.068 ± 0.336
4.026ThrArg: 4.026 ± 0.424
2.793ThrSer: 2.793 ± 0.906
3.368ThrThr: 3.368 ± 0.6
4.19ThrVal: 4.19 ± 0.587
1.15ThrTrp: 1.15 ± 0.309
1.479ThrTyr: 1.479 ± 0.602
0.0ThrXaa: 0.0 ± 0.0
Val
9.53ValAla: 9.53 ± 0.963
0.657ValCys: 0.657 ± 0.278
4.108ValAsp: 4.108 ± 0.565
4.765ValGlu: 4.765 ± 0.732
2.054ValPhe: 2.054 ± 0.409
4.683ValGly: 4.683 ± 0.882
1.397ValHis: 1.397 ± 0.294
2.793ValIle: 2.793 ± 0.541
1.314ValLys: 1.314 ± 0.275
7.065ValLeu: 7.065 ± 0.893
1.15ValMet: 1.15 ± 0.307
1.479ValAsn: 1.479 ± 0.456
3.286ValPro: 3.286 ± 0.524
2.3ValGln: 2.3 ± 0.468
5.587ValArg: 5.587 ± 0.654
4.19ValSer: 4.19 ± 0.886
5.751ValThr: 5.751 ± 0.686
4.354ValVal: 4.354 ± 0.906
1.068ValTrp: 1.068 ± 0.289
1.397ValTyr: 1.397 ± 0.726
0.0ValXaa: 0.0 ± 0.0
Trp
2.383TrpAla: 2.383 ± 0.428
0.082TrpCys: 0.082 ± 0.079
0.904TrpAsp: 0.904 ± 0.299
0.739TrpGlu: 0.739 ± 0.246
0.657TrpPhe: 0.657 ± 0.281
1.15TrpGly: 1.15 ± 0.235
0.411TrpHis: 0.411 ± 0.187
0.575TrpIle: 0.575 ± 0.204
0.493TrpLys: 0.493 ± 0.195
1.479TrpLeu: 1.479 ± 0.412
0.657TrpMet: 0.657 ± 0.217
0.411TrpAsn: 0.411 ± 0.168
1.068TrpPro: 1.068 ± 0.321
1.068TrpGln: 1.068 ± 0.318
2.465TrpArg: 2.465 ± 0.595
1.232TrpSer: 1.232 ± 0.308
1.397TrpThr: 1.397 ± 0.338
1.725TrpVal: 1.725 ± 0.387
0.246TrpTrp: 0.246 ± 0.121
0.411TrpTyr: 0.411 ± 0.157
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.697TyrAla: 3.697 ± 0.634
0.411TyrCys: 0.411 ± 0.189
1.561TyrAsp: 1.561 ± 0.311
1.15TyrGlu: 1.15 ± 0.295
0.575TyrPhe: 0.575 ± 0.245
1.479TyrGly: 1.479 ± 0.33
0.411TyrHis: 0.411 ± 0.176
0.493TyrIle: 0.493 ± 0.195
0.164TyrLys: 0.164 ± 0.111
2.054TyrLeu: 2.054 ± 0.35
0.329TyrMet: 0.329 ± 0.155
0.575TyrAsn: 0.575 ± 0.224
0.986TyrPro: 0.986 ± 0.265
0.575TyrGln: 0.575 ± 0.275
1.479TyrArg: 1.479 ± 0.274
1.068TyrSer: 1.068 ± 0.321
1.397TyrThr: 1.397 ± 0.336
1.397TyrVal: 1.397 ± 0.437
0.575TyrTrp: 0.575 ± 0.17
0.822TyrTyr: 0.822 ± 0.307
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 59 proteins (12173 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski