Amino acid dipepetide frequency for Streptococcus phage phi-SsuJS7_SSU0237

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.055AlaAla: 3.055 ± 0.728
0.555AlaCys: 0.555 ± 0.178
2.916AlaAsp: 2.916 ± 0.394
4.304AlaGlu: 4.304 ± 0.634
2.638AlaPhe: 2.638 ± 0.277
5.068AlaGly: 5.068 ± 0.723
0.833AlaHis: 0.833 ± 0.219
5.485AlaIle: 5.485 ± 0.889
6.248AlaLys: 6.248 ± 0.688
5.346AlaLeu: 5.346 ± 0.783
1.458AlaMet: 1.458 ± 0.34
3.402AlaAsn: 3.402 ± 0.542
2.152AlaPro: 2.152 ± 0.42
2.777AlaGln: 2.777 ± 0.492
2.985AlaArg: 2.985 ± 0.493
4.929AlaSer: 4.929 ± 0.829
3.957AlaThr: 3.957 ± 0.487
4.304AlaVal: 4.304 ± 0.74
0.972AlaTrp: 0.972 ± 0.281
3.332AlaTyr: 3.332 ± 0.385
0.0AlaXaa: 0.0 ± 0.0
Cys
0.625CysAla: 0.625 ± 0.214
0.208CysCys: 0.208 ± 0.099
0.278CysAsp: 0.278 ± 0.121
0.694CysGlu: 0.694 ± 0.157
0.347CysPhe: 0.347 ± 0.147
0.833CysGly: 0.833 ± 0.244
0.278CysHis: 0.278 ± 0.165
0.347CysIle: 0.347 ± 0.165
0.417CysLys: 0.417 ± 0.197
1.041CysLeu: 1.041 ± 0.296
0.139CysMet: 0.139 ± 0.1
0.347CysAsn: 0.347 ± 0.158
0.347CysPro: 0.347 ± 0.14
0.694CysGln: 0.694 ± 0.186
0.764CysArg: 0.764 ± 0.298
0.417CysSer: 0.417 ± 0.207
0.278CysThr: 0.278 ± 0.137
0.486CysVal: 0.486 ± 0.194
0.0CysTrp: 0.0 ± 0.0
0.625CysTyr: 0.625 ± 0.199
0.0CysXaa: 0.0 ± 0.0
Asp
3.402AspAla: 3.402 ± 0.437
0.555AspCys: 0.555 ± 0.226
3.332AspAsp: 3.332 ± 0.541
4.721AspGlu: 4.721 ± 0.766
3.471AspPhe: 3.471 ± 0.369
5.068AspGly: 5.068 ± 0.575
1.111AspHis: 1.111 ± 0.319
3.749AspIle: 3.749 ± 0.454
3.194AspLys: 3.194 ± 0.371
5.554AspLeu: 5.554 ± 0.802
2.013AspMet: 2.013 ± 0.305
2.499AspAsn: 2.499 ± 0.42
1.666AspPro: 1.666 ± 0.334
2.222AspGln: 2.222 ± 0.375
2.499AspArg: 2.499 ± 0.562
3.541AspSer: 3.541 ± 0.498
2.916AspThr: 2.916 ± 0.418
2.846AspVal: 2.846 ± 0.467
0.764AspTrp: 0.764 ± 0.188
3.471AspTyr: 3.471 ± 0.583
0.0AspXaa: 0.0 ± 0.0
Glu
5.068GluAla: 5.068 ± 0.547
0.555GluCys: 0.555 ± 0.189
4.304GluAsp: 4.304 ± 0.549
6.179GluGlu: 6.179 ± 0.878
2.291GluPhe: 2.291 ± 0.394
4.79GluGly: 4.79 ± 0.514
1.389GluHis: 1.389 ± 0.366
4.79GluIle: 4.79 ± 0.555
6.873GluLys: 6.873 ± 0.882
8.748GluLeu: 8.748 ± 1.041
1.944GluMet: 1.944 ± 0.416
3.124GluAsn: 3.124 ± 0.375
1.458GluPro: 1.458 ± 0.326
4.374GluGln: 4.374 ± 0.48
2.846GluArg: 2.846 ± 0.46
3.055GluSer: 3.055 ± 0.414
4.86GluThr: 4.86 ± 0.566
3.68GluVal: 3.68 ± 0.459
1.041GluTrp: 1.041 ± 0.3
1.944GluTyr: 1.944 ± 0.473
0.0GluXaa: 0.0 ± 0.0
Phe
2.013PheAla: 2.013 ± 0.463
0.625PheCys: 0.625 ± 0.193
2.985PheAsp: 2.985 ± 0.394
3.194PheGlu: 3.194 ± 0.529
1.527PhePhe: 1.527 ± 0.32
3.402PheGly: 3.402 ± 0.448
0.903PheHis: 0.903 ± 0.287
1.597PheIle: 1.597 ± 0.37
3.055PheLys: 3.055 ± 0.614
2.846PheLeu: 2.846 ± 0.54
0.972PheMet: 0.972 ± 0.259
2.222PheAsn: 2.222 ± 0.296
0.555PhePro: 0.555 ± 0.193
1.319PheGln: 1.319 ± 0.326
1.666PheArg: 1.666 ± 0.297
2.222PheSer: 2.222 ± 0.397
2.222PheThr: 2.222 ± 0.435
2.083PheVal: 2.083 ± 0.458
0.625PheTrp: 0.625 ± 0.209
1.736PheTyr: 1.736 ± 0.371
0.0PheXaa: 0.0 ± 0.0
Gly
3.263GlyAla: 3.263 ± 0.527
0.417GlyCys: 0.417 ± 0.216
4.027GlyAsp: 4.027 ± 0.518
3.68GlyGlu: 3.68 ± 0.411
3.055GlyPhe: 3.055 ± 0.424
4.027GlyGly: 4.027 ± 0.646
1.874GlyHis: 1.874 ± 0.345
5.693GlyIle: 5.693 ± 0.624
4.721GlyLys: 4.721 ± 0.496
5.762GlyLeu: 5.762 ± 0.939
1.805GlyMet: 1.805 ± 0.457
3.263GlyAsn: 3.263 ± 0.389
0.694GlyPro: 0.694 ± 0.18
2.708GlyGln: 2.708 ± 0.531
3.957GlyArg: 3.957 ± 0.486
3.61GlySer: 3.61 ± 0.428
4.166GlyThr: 4.166 ± 0.601
4.929GlyVal: 4.929 ± 0.663
0.625GlyTrp: 0.625 ± 0.162
2.708GlyTyr: 2.708 ± 0.408
0.069GlyXaa: 0.069 ± 0.058
His
1.041HisAla: 1.041 ± 0.243
0.208HisCys: 0.208 ± 0.104
0.903HisAsp: 0.903 ± 0.226
1.041HisGlu: 1.041 ± 0.301
0.764HisPhe: 0.764 ± 0.24
1.597HisGly: 1.597 ± 0.305
0.764HisHis: 0.764 ± 0.201
1.597HisIle: 1.597 ± 0.285
1.111HisLys: 1.111 ± 0.276
2.013HisLeu: 2.013 ± 0.274
0.417HisMet: 0.417 ± 0.178
1.25HisAsn: 1.25 ± 0.258
1.111HisPro: 1.111 ± 0.282
0.764HisGln: 0.764 ± 0.245
1.041HisArg: 1.041 ± 0.216
1.25HisSer: 1.25 ± 0.272
0.903HisThr: 0.903 ± 0.26
0.833HisVal: 0.833 ± 0.26
0.278HisTrp: 0.278 ± 0.118
0.625HisTyr: 0.625 ± 0.234
0.0HisXaa: 0.0 ± 0.0
Ile
4.651IleAla: 4.651 ± 0.371
0.625IleCys: 0.625 ± 0.21
5.415IleAsp: 5.415 ± 0.546
3.957IleGlu: 3.957 ± 0.424
1.597IlePhe: 1.597 ± 0.375
3.888IleGly: 3.888 ± 0.536
0.972IleHis: 0.972 ± 0.21
2.916IleIle: 2.916 ± 0.503
4.651IleLys: 4.651 ± 0.694
5.276IleLeu: 5.276 ± 0.551
1.041IleMet: 1.041 ± 0.224
2.777IleAsn: 2.777 ± 0.357
2.569IlePro: 2.569 ± 0.319
2.985IleGln: 2.985 ± 0.494
2.36IleArg: 2.36 ± 0.387
5.623IleSer: 5.623 ± 1.164
4.443IleThr: 4.443 ± 0.897
4.235IleVal: 4.235 ± 0.648
1.111IleTrp: 1.111 ± 0.303
2.499IleTyr: 2.499 ± 0.43
0.0IleXaa: 0.0 ± 0.0
Lys
6.109LysAla: 6.109 ± 0.579
0.417LysCys: 0.417 ± 0.156
3.68LysAsp: 3.68 ± 0.489
4.999LysGlu: 4.999 ± 0.598
1.666LysPhe: 1.666 ± 0.332
4.027LysGly: 4.027 ± 0.551
1.597LysHis: 1.597 ± 0.313
4.513LysIle: 4.513 ± 0.514
4.027LysLys: 4.027 ± 0.664
6.318LysLeu: 6.318 ± 0.774
2.013LysMet: 2.013 ± 0.464
2.708LysAsn: 2.708 ± 0.393
2.222LysPro: 2.222 ± 0.329
3.471LysGln: 3.471 ± 0.66
4.582LysArg: 4.582 ± 0.705
4.166LysSer: 4.166 ± 0.58
3.68LysThr: 3.68 ± 0.464
4.929LysVal: 4.929 ± 0.606
1.041LysTrp: 1.041 ± 0.281
2.36LysTyr: 2.36 ± 0.459
0.0LysXaa: 0.0 ± 0.0
Leu
6.595LeuAla: 6.595 ± 0.851
0.694LeuCys: 0.694 ± 0.179
5.832LeuAsp: 5.832 ± 0.51
7.428LeuGlu: 7.428 ± 0.838
3.055LeuPhe: 3.055 ± 0.428
5.346LeuGly: 5.346 ± 0.462
1.389LeuHis: 1.389 ± 0.336
4.443LeuIle: 4.443 ± 0.465
6.318LeuLys: 6.318 ± 0.59
7.428LeuLeu: 7.428 ± 0.856
1.805LeuMet: 1.805 ± 0.323
3.888LeuAsn: 3.888 ± 0.446
3.541LeuPro: 3.541 ± 0.518
3.888LeuGln: 3.888 ± 0.532
3.68LeuArg: 3.68 ± 0.49
6.734LeuSer: 6.734 ± 0.633
6.526LeuThr: 6.526 ± 0.756
6.804LeuVal: 6.804 ± 0.63
0.764LeuTrp: 0.764 ± 0.216
3.68LeuTyr: 3.68 ± 0.637
0.0LeuXaa: 0.0 ± 0.0
Met
2.013MetAla: 2.013 ± 0.335
0.208MetCys: 0.208 ± 0.088
1.666MetAsp: 1.666 ± 0.402
1.666MetGlu: 1.666 ± 0.341
0.972MetPhe: 0.972 ± 0.205
1.458MetGly: 1.458 ± 0.378
0.208MetHis: 0.208 ± 0.13
1.25MetIle: 1.25 ± 0.253
1.597MetLys: 1.597 ± 0.366
1.25MetLeu: 1.25 ± 0.328
0.764MetMet: 0.764 ± 0.235
0.764MetAsn: 0.764 ± 0.217
0.486MetPro: 0.486 ± 0.199
0.833MetGln: 0.833 ± 0.309
1.041MetArg: 1.041 ± 0.236
1.597MetSer: 1.597 ± 0.327
2.36MetThr: 2.36 ± 0.482
1.458MetVal: 1.458 ± 0.328
0.278MetTrp: 0.278 ± 0.143
0.625MetTyr: 0.625 ± 0.199
0.0MetXaa: 0.0 ± 0.0
Asn
4.235AsnAla: 4.235 ± 0.565
0.208AsnCys: 0.208 ± 0.11
2.499AsnAsp: 2.499 ± 0.443
2.777AsnGlu: 2.777 ± 0.531
2.152AsnPhe: 2.152 ± 0.364
4.929AsnGly: 4.929 ± 0.518
1.18AsnHis: 1.18 ± 0.275
2.083AsnIle: 2.083 ± 0.252
2.638AsnLys: 2.638 ± 0.39
4.096AsnLeu: 4.096 ± 0.818
0.833AsnMet: 0.833 ± 0.225
1.666AsnAsn: 1.666 ± 0.404
1.805AsnPro: 1.805 ± 0.414
2.013AsnGln: 2.013 ± 0.328
1.944AsnArg: 1.944 ± 0.416
2.291AsnSer: 2.291 ± 0.378
2.152AsnThr: 2.152 ± 0.504
2.013AsnVal: 2.013 ± 0.419
0.625AsnTrp: 0.625 ± 0.218
0.972AsnTyr: 0.972 ± 0.26
0.0AsnXaa: 0.0 ± 0.0
Pro
1.319ProAla: 1.319 ± 0.254
0.417ProCys: 0.417 ± 0.18
1.805ProAsp: 1.805 ± 0.384
2.638ProGlu: 2.638 ± 0.523
1.111ProPhe: 1.111 ± 0.29
0.833ProGly: 0.833 ± 0.42
0.903ProHis: 0.903 ± 0.212
2.083ProIle: 2.083 ± 0.302
2.638ProLys: 2.638 ± 0.451
2.708ProLeu: 2.708 ± 0.403
0.555ProMet: 0.555 ± 0.167
1.527ProAsn: 1.527 ± 0.336
0.903ProPro: 0.903 ± 0.32
0.972ProGln: 0.972 ± 0.276
1.666ProArg: 1.666 ± 0.302
2.291ProSer: 2.291 ± 0.405
2.36ProThr: 2.36 ± 0.346
2.013ProVal: 2.013 ± 0.353
0.347ProTrp: 0.347 ± 0.165
1.319ProTyr: 1.319 ± 0.346
0.0ProXaa: 0.0 ± 0.0
Gln
4.513GlnAla: 4.513 ± 0.595
0.347GlnCys: 0.347 ± 0.159
2.083GlnAsp: 2.083 ± 0.381
4.166GlnGlu: 4.166 ± 0.623
1.805GlnPhe: 1.805 ± 0.415
1.874GlnGly: 1.874 ± 0.316
0.555GlnHis: 0.555 ± 0.166
2.777GlnIle: 2.777 ± 0.471
2.499GlnLys: 2.499 ± 0.454
4.721GlnLeu: 4.721 ± 0.513
1.319GlnMet: 1.319 ± 0.319
1.805GlnAsn: 1.805 ± 0.348
1.25GlnPro: 1.25 ± 0.317
1.874GlnGln: 1.874 ± 0.309
1.389GlnArg: 1.389 ± 0.544
2.36GlnSer: 2.36 ± 0.384
3.055GlnThr: 3.055 ± 0.745
4.513GlnVal: 4.513 ± 0.47
0.694GlnTrp: 0.694 ± 0.242
0.764GlnTyr: 0.764 ± 0.239
0.0GlnXaa: 0.0 ± 0.0
Arg
2.083ArgAla: 2.083 ± 0.432
0.833ArgCys: 0.833 ± 0.241
2.43ArgAsp: 2.43 ± 0.403
2.985ArgGlu: 2.985 ± 0.358
1.666ArgPhe: 1.666 ± 0.356
2.499ArgGly: 2.499 ± 0.395
0.833ArgHis: 0.833 ± 0.259
3.402ArgIle: 3.402 ± 0.585
3.194ArgLys: 3.194 ± 0.542
5.137ArgLeu: 5.137 ± 0.591
1.041ArgMet: 1.041 ± 0.284
1.944ArgAsn: 1.944 ± 0.317
1.319ArgPro: 1.319 ± 0.286
2.777ArgGln: 2.777 ± 0.474
2.083ArgArg: 2.083 ± 0.502
2.569ArgSer: 2.569 ± 0.414
2.985ArgThr: 2.985 ± 0.646
3.263ArgVal: 3.263 ± 0.448
1.111ArgTrp: 1.111 ± 0.294
1.597ArgTyr: 1.597 ± 0.406
0.0ArgXaa: 0.0 ± 0.0
Ser
3.957SerAla: 3.957 ± 0.717
0.555SerCys: 0.555 ± 0.237
3.888SerAsp: 3.888 ± 0.398
4.929SerGlu: 4.929 ± 0.921
2.985SerPhe: 2.985 ± 0.576
4.929SerGly: 4.929 ± 0.579
1.319SerHis: 1.319 ± 0.285
4.721SerIle: 4.721 ± 0.857
3.818SerLys: 3.818 ± 0.506
5.693SerLeu: 5.693 ± 0.709
1.111SerMet: 1.111 ± 0.237
2.708SerAsn: 2.708 ± 0.458
2.083SerPro: 2.083 ± 0.341
2.985SerGln: 2.985 ± 0.787
3.194SerArg: 3.194 ± 0.527
4.999SerSer: 4.999 ± 1.052
4.096SerThr: 4.096 ± 0.729
3.957SerVal: 3.957 ± 0.416
0.972SerTrp: 0.972 ± 0.265
2.36SerTyr: 2.36 ± 0.392
0.0SerXaa: 0.0 ± 0.0
Thr
4.79ThrAla: 4.79 ± 0.514
0.139ThrCys: 0.139 ± 0.113
3.749ThrAsp: 3.749 ± 0.551
4.235ThrGlu: 4.235 ± 0.507
2.777ThrPhe: 2.777 ± 0.446
3.888ThrGly: 3.888 ± 0.616
0.833ThrHis: 0.833 ± 0.221
5.276ThrIle: 5.276 ± 0.724
4.651ThrLys: 4.651 ± 0.5
4.929ThrLeu: 4.929 ± 0.417
0.833ThrMet: 0.833 ± 0.202
2.083ThrAsn: 2.083 ± 0.582
2.36ThrPro: 2.36 ± 0.367
2.36ThrGln: 2.36 ± 0.63
2.083ThrArg: 2.083 ± 0.428
5.346ThrSer: 5.346 ± 1.196
4.999ThrThr: 4.999 ± 0.947
5.207ThrVal: 5.207 ± 0.725
0.972ThrTrp: 0.972 ± 0.23
2.291ThrTyr: 2.291 ± 0.47
0.0ThrXaa: 0.0 ± 0.0
Val
4.443ValAla: 4.443 ± 0.622
0.417ValCys: 0.417 ± 0.263
3.541ValAsp: 3.541 ± 0.424
5.623ValGlu: 5.623 ± 0.653
2.083ValPhe: 2.083 ± 0.37
3.541ValGly: 3.541 ± 0.551
1.319ValHis: 1.319 ± 0.255
4.166ValIle: 4.166 ± 0.606
3.957ValLys: 3.957 ± 0.499
6.526ValLeu: 6.526 ± 0.661
1.25ValMet: 1.25 ± 0.247
2.152ValAsn: 2.152 ± 0.367
2.43ValPro: 2.43 ± 0.412
2.36ValGln: 2.36 ± 0.367
3.61ValArg: 3.61 ± 0.521
4.651ValSer: 4.651 ± 0.723
4.721ValThr: 4.721 ± 0.457
3.194ValVal: 3.194 ± 0.441
1.111ValTrp: 1.111 ± 0.301
2.43ValTyr: 2.43 ± 0.494
0.0ValXaa: 0.0 ± 0.0
Trp
1.111TrpAla: 1.111 ± 0.28
0.208TrpCys: 0.208 ± 0.137
0.417TrpAsp: 0.417 ± 0.146
1.25TrpGlu: 1.25 ± 0.246
0.764TrpPhe: 0.764 ± 0.275
0.833TrpGly: 0.833 ± 0.185
0.347TrpHis: 0.347 ± 0.154
0.694TrpIle: 0.694 ± 0.209
0.417TrpLys: 0.417 ± 0.195
1.111TrpLeu: 1.111 ± 0.223
0.486TrpMet: 0.486 ± 0.144
1.18TrpAsn: 1.18 ± 0.291
0.069TrpPro: 0.069 ± 0.065
0.833TrpGln: 0.833 ± 0.205
0.625TrpArg: 0.625 ± 0.254
1.041TrpSer: 1.041 ± 0.249
1.041TrpThr: 1.041 ± 0.258
0.833TrpVal: 0.833 ± 0.233
0.278TrpTrp: 0.278 ± 0.147
0.347TrpTyr: 0.347 ± 0.123
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.43TyrAla: 2.43 ± 0.319
0.972TyrCys: 0.972 ± 0.307
2.846TyrAsp: 2.846 ± 0.567
2.846TyrGlu: 2.846 ± 0.465
1.041TyrPhe: 1.041 ± 0.311
2.222TyrGly: 2.222 ± 0.385
0.903TyrHis: 0.903 ± 0.293
2.013TyrIle: 2.013 ± 0.407
2.499TyrLys: 2.499 ± 0.468
3.332TyrLeu: 3.332 ± 0.468
0.625TyrMet: 0.625 ± 0.239
1.805TyrAsn: 1.805 ± 0.379
1.389TyrPro: 1.389 ± 0.34
2.152TyrGln: 2.152 ± 0.352
1.736TyrArg: 1.736 ± 0.354
2.569TyrSer: 2.569 ± 0.376
2.013TyrThr: 2.013 ± 0.368
2.013TyrVal: 2.013 ± 0.404
0.278TyrTrp: 0.278 ± 0.108
1.736TyrTyr: 1.736 ± 0.512
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.069XaaThr: 0.069 ± 0.058
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
17.842XaaXaa: 17.842 ± 14.909
Statistics based on 52 proteins (14405 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski