Amino acid dipepetide frequency for Lactobacillus iners DSM 13335

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.713AlaAla: 4.713 ± 0.168
0.589AlaCys: 0.589 ± 0.044
4.257AlaAsp: 4.257 ± 0.149
3.722AlaGlu: 3.722 ± 0.133
2.766AlaPhe: 2.766 ± 0.103
4.498AlaGly: 4.498 ± 0.112
1.326AlaHis: 1.326 ± 0.049
5.988AlaIle: 5.988 ± 0.131
5.939AlaLys: 5.939 ± 0.18
6.392AlaLeu: 6.392 ± 0.149
1.745AlaMet: 1.745 ± 0.072
3.859AlaAsn: 3.859 ± 0.118
1.852AlaPro: 1.852 ± 0.07
2.562AlaGln: 2.562 ± 0.083
2.255AlaArg: 2.255 ± 0.088
3.746AlaSer: 3.746 ± 0.089
4.026AlaThr: 4.026 ± 0.12
4.286AlaVal: 4.286 ± 0.127
0.511AlaTrp: 0.511 ± 0.037
2.447AlaTyr: 2.447 ± 0.093
0.0AlaXaa: 0.0 ± 0.0
Cys
0.474CysAla: 0.474 ± 0.038
0.113CysCys: 0.113 ± 0.019
0.521CysAsp: 0.521 ± 0.036
0.385CysGlu: 0.385 ± 0.033
0.393CysPhe: 0.393 ± 0.036
0.631CysGly: 0.631 ± 0.045
0.207CysHis: 0.207 ± 0.023
0.652CysIle: 0.652 ± 0.041
0.527CysLys: 0.527 ± 0.042
0.88CysLeu: 0.88 ± 0.056
0.196CysMet: 0.196 ± 0.024
0.443CysAsn: 0.443 ± 0.04
0.291CysPro: 0.291 ± 0.032
0.267CysGln: 0.267 ± 0.026
0.22CysArg: 0.22 ± 0.025
0.521CysSer: 0.521 ± 0.036
0.401CysThr: 0.401 ± 0.036
0.466CysVal: 0.466 ± 0.039
0.094CysTrp: 0.094 ± 0.017
0.314CysTyr: 0.314 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
3.901AspAla: 3.901 ± 0.119
0.417AspCys: 0.417 ± 0.035
3.523AspAsp: 3.523 ± 0.126
3.94AspGlu: 3.94 ± 0.115
2.855AspPhe: 2.855 ± 0.09
3.337AspGly: 3.337 ± 0.138
1.252AspHis: 1.252 ± 0.061
5.058AspIle: 5.058 ± 0.143
5.024AspLys: 5.024 ± 0.158
5.619AspLeu: 5.619 ± 0.136
1.509AspMet: 1.509 ± 0.057
3.434AspAsn: 3.434 ± 0.137
2.389AspPro: 2.389 ± 0.124
2.3AspGln: 2.3 ± 0.083
2.056AspArg: 2.056 ± 0.077
3.193AspSer: 3.193 ± 0.099
2.798AspThr: 2.798 ± 0.117
3.591AspVal: 3.591 ± 0.114
0.542AspTrp: 0.542 ± 0.044
2.515AspTyr: 2.515 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
3.822GluAla: 3.822 ± 0.135
0.388GluCys: 0.388 ± 0.033
3.07GluAsp: 3.07 ± 0.1
3.332GluGlu: 3.332 ± 0.101
2.263GluPhe: 2.263 ± 0.079
2.769GluGly: 2.769 ± 0.109
1.205GluHis: 1.205 ± 0.055
5.043GluIle: 5.043 ± 0.129
5.25GluLys: 5.25 ± 0.148
5.792GluLeu: 5.792 ± 0.139
1.498GluMet: 1.498 ± 0.062
3.513GluAsn: 3.513 ± 0.102
1.653GluPro: 1.653 ± 0.089
2.557GluGln: 2.557 ± 0.085
2.069GluArg: 2.069 ± 0.083
2.69GluSer: 2.69 ± 0.088
2.672GluThr: 2.672 ± 0.097
3.704GluVal: 3.704 ± 0.106
0.44GluTrp: 0.44 ± 0.036
2.276GluTyr: 2.276 ± 0.074
0.0GluXaa: 0.0 ± 0.0
Phe
2.774PheAla: 2.774 ± 0.092
0.44PheCys: 0.44 ± 0.034
2.756PheAsp: 2.756 ± 0.085
2.381PheGlu: 2.381 ± 0.084
2.124PhePhe: 2.124 ± 0.088
2.853PheGly: 2.853 ± 0.091
0.644PheHis: 0.644 ± 0.042
3.733PheIle: 3.733 ± 0.133
3.167PheLys: 3.167 ± 0.097
4.351PheLeu: 4.351 ± 0.14
1.119PheMet: 1.119 ± 0.06
2.604PheAsn: 2.604 ± 0.094
1.242PhePro: 1.242 ± 0.063
1.2PheGln: 1.2 ± 0.056
1.231PheArg: 1.231 ± 0.054
3.196PheSer: 3.196 ± 0.112
2.47PheThr: 2.47 ± 0.089
3.023PheVal: 3.023 ± 0.092
0.458PheTrp: 0.458 ± 0.038
1.881PheTyr: 1.881 ± 0.071
0.0PheXaa: 0.0 ± 0.0
Gly
3.867GlyAla: 3.867 ± 0.105
0.529GlyCys: 0.529 ± 0.045
3.188GlyAsp: 3.188 ± 0.097
3.081GlyGlu: 3.081 ± 0.094
2.675GlyPhe: 2.675 ± 0.099
3.736GlyGly: 3.736 ± 0.107
1.341GlyHis: 1.341 ± 0.052
5.664GlyIle: 5.664 ± 0.147
5.174GlyLys: 5.174 ± 0.149
5.988GlyLeu: 5.988 ± 0.152
1.69GlyMet: 1.69 ± 0.088
3.023GlyAsn: 3.023 ± 0.105
1.315GlyPro: 1.315 ± 0.052
2.331GlyGln: 2.331 ± 0.108
2.41GlyArg: 2.41 ± 0.083
3.633GlySer: 3.633 ± 0.101
3.518GlyThr: 3.518 ± 0.143
4.199GlyVal: 4.199 ± 0.116
0.55GlyTrp: 0.55 ± 0.042
2.583GlyTyr: 2.583 ± 0.086
0.0GlyXaa: 0.0 ± 0.0
His
1.21HisAla: 1.21 ± 0.049
0.186HisCys: 0.186 ± 0.02
1.134HisAsp: 1.134 ± 0.051
1.168HisGlu: 1.168 ± 0.061
0.954HisPhe: 0.954 ± 0.048
1.383HisGly: 1.383 ± 0.068
0.503HisHis: 0.503 ± 0.043
1.564HisIle: 1.564 ± 0.065
1.396HisLys: 1.396 ± 0.061
1.815HisLeu: 1.815 ± 0.078
0.511HisMet: 0.511 ± 0.035
1.25HisAsn: 1.25 ± 0.056
0.893HisPro: 0.893 ± 0.043
0.888HisGln: 0.888 ± 0.05
0.809HisArg: 0.809 ± 0.053
1.171HisSer: 1.171 ± 0.064
0.914HisThr: 0.914 ± 0.044
1.37HisVal: 1.37 ± 0.058
0.16HisTrp: 0.16 ± 0.018
0.87HisTyr: 0.87 ± 0.049
0.0HisXaa: 0.0 ± 0.0
Ile
6.494IleAla: 6.494 ± 0.157
0.833IleCys: 0.833 ± 0.053
5.383IleAsp: 5.383 ± 0.128
4.328IleGlu: 4.328 ± 0.106
4.073IlePhe: 4.073 ± 0.131
4.948IleGly: 4.948 ± 0.139
1.543IleHis: 1.543 ± 0.066
7.586IleIle: 7.586 ± 0.189
7.013IleLys: 7.013 ± 0.152
8.223IleLeu: 8.223 ± 0.231
2.049IleMet: 2.049 ± 0.079
5.386IleAsn: 5.386 ± 0.143
3.036IlePro: 3.036 ± 0.09
2.415IleGln: 2.415 ± 0.093
2.819IleArg: 2.819 ± 0.087
6.138IleSer: 6.138 ± 0.174
4.61IleThr: 4.61 ± 0.118
5.831IleVal: 5.831 ± 0.135
0.608IleTrp: 0.608 ± 0.038
3.136IleTyr: 3.136 ± 0.102
0.0IleXaa: 0.0 ± 0.0
Lys
5.087LysAla: 5.087 ± 0.19
0.511LysCys: 0.511 ± 0.04
4.794LysAsp: 4.794 ± 0.191
4.959LysGlu: 4.959 ± 0.129
2.863LysPhe: 2.863 ± 0.083
3.992LysGly: 3.992 ± 0.109
1.595LysHis: 1.595 ± 0.059
7.558LysIle: 7.558 ± 0.155
7.979LysLys: 7.979 ± 0.202
7.767LysLeu: 7.767 ± 0.18
2.698LysMet: 2.698 ± 0.088
5.509LysAsn: 5.509 ± 0.123
2.554LysPro: 2.554 ± 0.118
3.788LysGln: 3.788 ± 0.116
3.138LysArg: 3.138 ± 0.108
4.155LysSer: 4.155 ± 0.109
4.487LysThr: 4.487 ± 0.202
5.197LysVal: 5.197 ± 0.159
0.595LysTrp: 0.595 ± 0.041
3.594LysTyr: 3.594 ± 0.103
0.0LysXaa: 0.0 ± 0.0
Leu
7.251LeuAla: 7.251 ± 0.191
0.739LeuCys: 0.739 ± 0.046
5.506LeuAsp: 5.506 ± 0.138
4.925LeuGlu: 4.925 ± 0.125
4.241LeuPhe: 4.241 ± 0.139
6.049LeuGly: 6.049 ± 0.15
1.912LeuHis: 1.912 ± 0.075
8.105LeuIle: 8.105 ± 0.185
7.764LeuLys: 7.764 ± 0.163
9.541LeuLeu: 9.541 ± 0.246
2.276LeuMet: 2.276 ± 0.094
5.509LeuAsn: 5.509 ± 0.139
3.534LeuPro: 3.534 ± 0.091
3.576LeuGln: 3.576 ± 0.125
3.411LeuArg: 3.411 ± 0.103
6.971LeuSer: 6.971 ± 0.135
5.478LeuThr: 5.478 ± 0.128
6.266LeuVal: 6.266 ± 0.146
0.713LeuTrp: 0.713 ± 0.044
2.994LeuTyr: 2.994 ± 0.109
0.0LeuXaa: 0.0 ± 0.0
Met
1.873MetAla: 1.873 ± 0.084
0.215MetCys: 0.215 ± 0.023
1.438MetAsp: 1.438 ± 0.061
1.239MetGlu: 1.239 ± 0.06
1.009MetPhe: 1.009 ± 0.054
1.553MetGly: 1.553 ± 0.075
0.589MetHis: 0.589 ± 0.034
2.122MetIle: 2.122 ± 0.084
2.062MetLys: 2.062 ± 0.081
2.41MetLeu: 2.41 ± 0.089
0.781MetMet: 0.781 ± 0.051
1.391MetAsn: 1.391 ± 0.06
1.098MetPro: 1.098 ± 0.053
1.108MetGln: 1.108 ± 0.056
0.94MetArg: 0.94 ± 0.048
1.661MetSer: 1.661 ± 0.065
1.514MetThr: 1.514 ± 0.076
1.65MetVal: 1.65 ± 0.064
0.17MetTrp: 0.17 ± 0.025
0.757MetTyr: 0.757 ± 0.05
0.0MetXaa: 0.0 ± 0.0
Asn
3.434AsnAla: 3.434 ± 0.1
0.5AsnCys: 0.5 ± 0.043
3.413AsnAsp: 3.413 ± 0.116
3.264AsnGlu: 3.264 ± 0.106
2.525AsnPhe: 2.525 ± 0.087
3.667AsnGly: 3.667 ± 0.144
1.223AsnHis: 1.223 ± 0.054
5.286AsnIle: 5.286 ± 0.143
4.975AsnLys: 4.975 ± 0.131
5.472AsnLeu: 5.472 ± 0.145
1.546AsnMet: 1.546 ± 0.069
3.864AsnAsn: 3.864 ± 0.122
2.185AsnPro: 2.185 ± 0.1
2.525AsnGln: 2.525 ± 0.086
1.933AsnArg: 1.933 ± 0.073
3.497AsnSer: 3.497 ± 0.107
2.677AsnThr: 2.677 ± 0.093
3.335AsnVal: 3.335 ± 0.094
0.524AsnTrp: 0.524 ± 0.036
2.562AsnTyr: 2.562 ± 0.093
0.0AsnXaa: 0.0 ± 0.0
Pro
2.185ProAla: 2.185 ± 0.094
0.181ProCys: 0.181 ± 0.019
2.185ProAsp: 2.185 ± 0.114
2.546ProGlu: 2.546 ± 0.131
1.561ProPhe: 1.561 ± 0.072
1.805ProGly: 1.805 ± 0.074
0.665ProHis: 0.665 ± 0.047
2.635ProIle: 2.635 ± 0.097
2.837ProLys: 2.837 ± 0.186
3.018ProLeu: 3.018 ± 0.088
0.699ProMet: 0.699 ± 0.045
1.836ProAsn: 1.836 ± 0.074
0.503ProPro: 0.503 ± 0.038
1.43ProGln: 1.43 ± 0.068
1.019ProArg: 1.019 ± 0.057
1.894ProSer: 1.894 ± 0.098
1.986ProThr: 1.986 ± 0.111
2.308ProVal: 2.308 ± 0.113
0.251ProTrp: 0.251 ± 0.025
1.286ProTyr: 1.286 ± 0.059
0.0ProXaa: 0.0 ± 0.0
Gln
2.871GlnAla: 2.871 ± 0.094
0.231GlnCys: 0.231 ± 0.021
1.944GlnAsp: 1.944 ± 0.078
2.355GlnGlu: 2.355 ± 0.083
1.564GlnPhe: 1.564 ± 0.07
2.072GlnGly: 2.072 ± 0.082
0.796GlnHis: 0.796 ± 0.046
3.335GlnIle: 3.335 ± 0.092
3.626GlnLys: 3.626 ± 0.107
4.241GlnLeu: 4.241 ± 0.128
1.053GlnMet: 1.053 ± 0.069
2.219GlnAsn: 2.219 ± 0.08
1.153GlnPro: 1.153 ± 0.064
1.732GlnGln: 1.732 ± 0.084
1.585GlnArg: 1.585 ± 0.063
1.975GlnSer: 1.975 ± 0.089
2.007GlnThr: 2.007 ± 0.088
2.606GlnVal: 2.606 ± 0.098
0.377GlnTrp: 0.377 ± 0.038
1.506GlnTyr: 1.506 ± 0.059
0.0GlnXaa: 0.0 ± 0.0
Arg
2.179ArgAla: 2.179 ± 0.081
0.241ArgCys: 0.241 ± 0.026
1.978ArgAsp: 1.978 ± 0.082
2.096ArgGlu: 2.096 ± 0.099
1.525ArgPhe: 1.525 ± 0.065
2.033ArgGly: 2.033 ± 0.095
0.802ArgHis: 0.802 ± 0.049
3.088ArgIle: 3.088 ± 0.092
3.068ArgLys: 3.068 ± 0.098
3.455ArgLeu: 3.455 ± 0.117
1.085ArgMet: 1.085 ± 0.057
1.933ArgAsn: 1.933 ± 0.073
1.168ArgPro: 1.168 ± 0.064
1.632ArgGln: 1.632 ± 0.07
1.847ArgArg: 1.847 ± 0.084
1.938ArgSer: 1.938 ± 0.072
1.737ArgThr: 1.737 ± 0.073
2.295ArgVal: 2.295 ± 0.089
0.322ArgTrp: 0.322 ± 0.033
1.546ArgTyr: 1.546 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
3.743SerAla: 3.743 ± 0.108
0.485SerCys: 0.485 ± 0.035
3.887SerAsp: 3.887 ± 0.116
3.445SerGlu: 3.445 ± 0.101
2.827SerPhe: 2.827 ± 0.086
4.186SerGly: 4.186 ± 0.101
1.187SerHis: 1.187 ± 0.062
5.017SerIle: 5.017 ± 0.138
4.781SerLys: 4.781 ± 0.136
5.886SerLeu: 5.886 ± 0.129
1.467SerMet: 1.467 ± 0.06
3.411SerAsn: 3.411 ± 0.105
1.559SerPro: 1.559 ± 0.061
2.47SerGln: 2.47 ± 0.097
2.214SerArg: 2.214 ± 0.076
3.667SerSer: 3.667 ± 0.11
3.112SerThr: 3.112 ± 0.085
3.961SerVal: 3.961 ± 0.124
0.558SerTrp: 0.558 ± 0.039
2.496SerTyr: 2.496 ± 0.088
0.0SerXaa: 0.0 ± 0.0
Thr
3.722ThrAla: 3.722 ± 0.157
0.443ThrCys: 0.443 ± 0.033
3.256ThrAsp: 3.256 ± 0.138
2.606ThrGlu: 2.606 ± 0.094
2.441ThrPhe: 2.441 ± 0.087
3.675ThrGly: 3.675 ± 0.122
1.035ThrHis: 1.035 ± 0.055
5.019ThrIle: 5.019 ± 0.131
4.071ThrLys: 4.071 ± 0.137
4.969ThrLeu: 4.969 ± 0.099
1.202ThrMet: 1.202 ± 0.056
2.981ThrAsn: 2.981 ± 0.089
2.316ThrPro: 2.316 ± 0.157
1.81ThrGln: 1.81 ± 0.08
1.708ThrArg: 1.708 ± 0.073
3.125ThrSer: 3.125 ± 0.091
3.175ThrThr: 3.175 ± 0.118
3.767ThrVal: 3.767 ± 0.206
0.427ThrTrp: 0.427 ± 0.03
1.986ThrTyr: 1.986 ± 0.084
0.0ThrXaa: 0.0 ± 0.0
Val
5.195ValAla: 5.195 ± 0.126
0.519ValCys: 0.519 ± 0.04
4.144ValAsp: 4.144 ± 0.111
3.84ValGlu: 3.84 ± 0.116
2.51ValPhe: 2.51 ± 0.083
4.383ValGly: 4.383 ± 0.106
1.098ValHis: 1.098 ± 0.059
5.323ValIle: 5.323 ± 0.134
5.108ValLys: 5.108 ± 0.182
5.619ValLeu: 5.619 ± 0.125
1.464ValMet: 1.464 ± 0.068
3.568ValAsn: 3.568 ± 0.107
2.528ValPro: 2.528 ± 0.159
2.245ValGln: 2.245 ± 0.082
2.248ValArg: 2.248 ± 0.084
4.246ValSer: 4.246 ± 0.12
3.914ValThr: 3.914 ± 0.192
4.867ValVal: 4.867 ± 0.133
0.524ValTrp: 0.524 ± 0.035
2.187ValTyr: 2.187 ± 0.08
0.0ValXaa: 0.0 ± 0.0
Trp
0.443TrpAla: 0.443 ± 0.037
0.079TrpCys: 0.079 ± 0.015
0.411TrpAsp: 0.411 ± 0.042
0.338TrpGlu: 0.338 ± 0.032
0.414TrpPhe: 0.414 ± 0.036
0.508TrpGly: 0.508 ± 0.034
0.233TrpHis: 0.233 ± 0.027
0.705TrpIle: 0.705 ± 0.046
0.516TrpLys: 0.516 ± 0.043
1.027TrpLeu: 1.027 ± 0.059
0.207TrpMet: 0.207 ± 0.024
0.527TrpAsn: 0.527 ± 0.043
0.251TrpPro: 0.251 ± 0.024
0.492TrpGln: 0.492 ± 0.034
0.33TrpArg: 0.33 ± 0.031
0.495TrpSer: 0.495 ± 0.038
0.375TrpThr: 0.375 ± 0.035
0.458TrpVal: 0.458 ± 0.039
0.094TrpTrp: 0.094 ± 0.017
0.377TrpTyr: 0.377 ± 0.033
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.24TyrAla: 2.24 ± 0.079
0.362TyrCys: 0.362 ± 0.035
2.465TyrAsp: 2.465 ± 0.085
2.059TyrGlu: 2.059 ± 0.079
2.064TyrPhe: 2.064 ± 0.08
2.415TyrGly: 2.415 ± 0.08
0.993TyrHis: 0.993 ± 0.053
2.971TyrIle: 2.971 ± 0.081
2.546TyrLys: 2.546 ± 0.088
4.294TyrLeu: 4.294 ± 0.132
0.783TyrMet: 0.783 ± 0.039
2.151TyrAsn: 2.151 ± 0.084
1.312TyrPro: 1.312 ± 0.059
1.918TyrGln: 1.918 ± 0.079
1.724TyrArg: 1.724 ± 0.071
2.444TyrSer: 2.444 ± 0.077
1.865TyrThr: 1.865 ± 0.078
2.347TyrVal: 2.347 ± 0.072
0.362TyrTrp: 0.362 ± 0.029
1.687TyrTyr: 1.687 ± 0.064
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1214 proteins (381740 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski