Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Kineosporiales; Kineosporiaceae; Kineococcus; Kineococcus radiotolerans

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4674 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A6WF53|A6WF53_KINRD Exodeoxyribonuclease III OS=Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) OX=266940 GN=Krad_3979 PE=3 SV=1
MM1 pKa = 7.18TNHH4 pKa = 6.39RR5 pKa = 11.84PSYY8 pKa = 9.76EE9 pKa = 3.83GRR11 pKa = 11.84PLPRR15 pKa = 11.84PAEE18 pKa = 4.32DD19 pKa = 4.36VDD21 pKa = 4.04DD22 pKa = 4.07QGLAFDD28 pKa = 5.15LATVATRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84ALGLLGLGAAGLTLAACGDD56 pKa = 4.12DD57 pKa = 3.48ATASSSTSSSAPTSGALTEE76 pKa = 4.91IPDD79 pKa = 3.97EE80 pKa = 4.36TAGPYY85 pKa = 10.2PGDD88 pKa = 3.58GSNGVDD94 pKa = 3.16VLEE97 pKa = 4.47RR98 pKa = 11.84SGVVRR103 pKa = 11.84SDD105 pKa = 2.68IRR107 pKa = 11.84SSFGTSTTTAEE118 pKa = 4.42GVPMTLTLTVVDD130 pKa = 4.43LAGGNVPFEE139 pKa = 4.26GVAVYY144 pKa = 9.93VWHH147 pKa = 7.6CDD149 pKa = 3.18RR150 pKa = 11.84DD151 pKa = 4.18GEE153 pKa = 4.27YY154 pKa = 10.88SLYY157 pKa = 10.93SEE159 pKa = 6.18DD160 pKa = 4.83IADD163 pKa = 3.7EE164 pKa = 4.34NYY166 pKa = 10.64LRR168 pKa = 11.84GVQVADD174 pKa = 3.4ADD176 pKa = 4.32GNVTFTSVFPACYY189 pKa = 9.56DD190 pKa = 3.41GRR192 pKa = 11.84WPHH195 pKa = 5.21VHH197 pKa = 6.5FEE199 pKa = 4.27VYY201 pKa = 10.21PDD203 pKa = 3.78AEE205 pKa = 4.84SITDD209 pKa = 3.55VANCIATSQVALPQEE224 pKa = 4.22ACEE227 pKa = 4.05AVYY230 pKa = 8.64ATTGYY235 pKa = 8.88EE236 pKa = 3.81QSVTNLARR244 pKa = 11.84VTLATDD250 pKa = 3.33NVFGDD255 pKa = 4.76DD256 pKa = 4.09GGASQTPTSSGDD268 pKa = 3.25VTNGYY273 pKa = 10.59AIALTVGVDD282 pKa = 3.39TTTEE286 pKa = 3.98PTAGTMAGMGSDD298 pKa = 3.54SSGHH302 pKa = 6.45PGGGPGGGGTPPSGAPAAAVSAAA325 pKa = 3.57

Molecular weight: 32.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A6WGN4|A6WGN4_KINRD Uncharacterized protein OS=Kineococcus radiotolerans (strain ATCC BAA-149 / DSM 14245 / SRS30216) OX=266940 GN=Krad_4514 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILSARR35 pKa = 11.84RR36 pKa = 11.84TKK38 pKa = 10.67GRR40 pKa = 11.84AQISAA45 pKa = 3.65

Molecular weight: 5.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4674

0

4674

1488609

27

1995

318.5

33.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.636 ± 0.044

0.645 ± 0.009

6.031 ± 0.029

5.374 ± 0.036

2.543 ± 0.019

9.545 ± 0.034

2.221 ± 0.019

2.397 ± 0.026

1.336 ± 0.02

10.754 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.367 ± 0.012

1.436 ± 0.017

6.17 ± 0.032

2.736 ± 0.022

8.447 ± 0.039

5.086 ± 0.022

6.167 ± 0.028

10.046 ± 0.04

1.469 ± 0.014

1.594 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski