Amino acid dipepetide frequency for Pseudo-nitzschia multistriata

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.868AlaAla: 8.868 ± 0.074	1.222AlaCys: 1.222 ± 0.017	3.828AlaAsp: 3.828 ± 0.031	4.888AlaGlu: 4.888 ± 0.044	2.886AlaPhe: 2.886 ± 0.025	5.372AlaGly: 5.372 ± 0.043	1.348AlaHis: 1.348 ± 0.014	3.795AlaIle: 3.795 ± 0.029	4.23AlaLys: 4.23 ± 0.034	7.069AlaLeu: 7.069 ± 0.045	2.078AlaMet: 2.078 ± 0.02	3.044AlaAsn: 3.044 ± 0.025	4.144AlaPro: 4.144 ± 0.051	2.265AlaGln: 2.265 ± 0.023	4.52AlaArg: 4.52 ± 0.037	7.667AlaSer: 7.667 ± 0.042	5.448AlaThr: 5.448 ± 0.035	5.064AlaVal: 5.064 ± 0.037	0.795AlaTrp: 0.795 ± 0.012	1.662AlaTyr: 1.662 ± 0.017	0.228AlaXaa: 0.228 ± 0.008
Cys	1.027CysAla: 1.027 ± 0.015	0.434CysCys: 0.434 ± 0.011	0.956CysAsp: 0.956 ± 0.014	0.82CysGlu: 0.82 ± 0.013	0.653CysPhe: 0.653 ± 0.011	1.204CysGly: 1.204 ± 0.017	0.406CysHis: 0.406 ± 0.009	0.699CysIle: 0.699 ± 0.012	0.671CysLys: 0.671 ± 0.01	1.456CysLeu: 1.456 ± 0.019	0.314CysMet: 0.314 ± 0.007	0.636CysAsn: 0.636 ± 0.01	0.771CysPro: 0.771 ± 0.014	0.5CysGln: 0.5 ± 0.01	0.975CysArg: 0.975 ± 0.012	1.277CysSer: 1.277 ± 0.017	0.824CysThr: 0.824 ± 0.014	0.967CysVal: 0.967 ± 0.014	0.214CysTrp: 0.214 ± 0.006	0.424CysTyr: 0.424 ± 0.009	0.034CysXaa: 0.034 ± 0.002
Asp	4.999AspAla: 4.999 ± 0.034	0.936AspCys: 0.936 ± 0.011	6.495AspAsp: 6.495 ± 0.073	5.492AspGlu: 5.492 ± 0.043	2.19AspPhe: 2.19 ± 0.022	5.174AspGly: 5.174 ± 0.035	1.375AspHis: 1.375 ± 0.016	2.574AspIle: 2.574 ± 0.021	2.637AspLys: 2.637 ± 0.027	4.778AspLeu: 4.778 ± 0.032	1.152AspMet: 1.152 ± 0.014	2.628AspAsn: 2.628 ± 0.028	3.326AspPro: 3.326 ± 0.028	1.804AspGln: 1.804 ± 0.018	3.483AspArg: 3.483 ± 0.026	4.915AspSer: 4.915 ± 0.037	2.975AspThr: 2.975 ± 0.023	3.591AspVal: 3.591 ± 0.028	0.742AspTrp: 0.742 ± 0.012	1.633AspTyr: 1.633 ± 0.018	0.204AspXaa: 0.204 ± 0.006
Glu	5.746GluAla: 5.746 ± 0.045	0.888GluCys: 0.888 ± 0.013	5.077GluAsp: 5.077 ± 0.042	7.058GluGlu: 7.058 ± 0.069	1.971GluPhe: 1.971 ± 0.02	4.23GluGly: 4.23 ± 0.026	1.431GluHis: 1.431 ± 0.019	3.221GluIle: 3.221 ± 0.026	4.361GluLys: 4.361 ± 0.045	5.257GluLeu: 5.257 ± 0.047	1.55GluMet: 1.55 ± 0.02	3.308GluAsn: 3.308 ± 0.026	2.533GluPro: 2.533 ± 0.026	2.685GluGln: 2.685 ± 0.028	4.321GluArg: 4.321 ± 0.04	5.302GluSer: 5.302 ± 0.038	4.219GluThr: 4.219 ± 0.03	3.389GluVal: 3.389 ± 0.028	0.808GluTrp: 0.808 ± 0.01	1.635GluTyr: 1.635 ± 0.015	0.205GluXaa: 0.205 ± 0.007
Phe	2.653PheAla: 2.653 ± 0.022	0.785PheCys: 0.785 ± 0.015	2.359PheAsp: 2.359 ± 0.024	2.151PheGlu: 2.151 ± 0.022	1.608PhePhe: 1.608 ± 0.019	2.864PheGly: 2.864 ± 0.027	0.865PheHis: 0.865 ± 0.014	1.118PheIle: 1.118 ± 0.016	1.171PheLys: 1.171 ± 0.016	3.562PheLeu: 3.562 ± 0.027	0.662PheMet: 0.662 ± 0.01	1.058PheAsn: 1.058 ± 0.014	1.687PhePro: 1.687 ± 0.017	1.25PheGln: 1.25 ± 0.016	2.203PheArg: 2.203 ± 0.019	2.993PheSer: 2.993 ± 0.023	1.469PheThr: 1.469 ± 0.016	2.615PheVal: 2.615 ± 0.025	0.475PheTrp: 0.475 ± 0.009	0.972PheTyr: 0.972 ± 0.015	0.076PheXaa: 0.076 ± 0.004
Gly	5.236GlyAla: 5.236 ± 0.044	1.113GlyCys: 1.113 ± 0.014	4.754GlyAsp: 4.754 ± 0.035	4.271GlyGlu: 4.271 ± 0.031	2.607GlyPhe: 2.607 ± 0.02	7.181GlyGly: 7.181 ± 0.063	1.43GlyHis: 1.43 ± 0.016	3.477GlyIle: 3.477 ± 0.03	3.886GlyLys: 3.886 ± 0.033	5.171GlyLeu: 5.171 ± 0.036	1.625GlyMet: 1.625 ± 0.019	3.63GlyAsn: 3.63 ± 0.027	2.78GlyPro: 2.78 ± 0.024	1.86GlyGln: 1.86 ± 0.02	4.355GlyArg: 4.355 ± 0.036	6.693GlySer: 6.693 ± 0.045	4.281GlyThr: 4.281 ± 0.032	4.073GlyVal: 4.073 ± 0.029	0.851GlyTrp: 0.851 ± 0.013	1.73GlyTyr: 1.73 ± 0.02	0.205GlyXaa: 0.205 ± 0.007
His	1.529HisAla: 1.529 ± 0.018	0.409HisCys: 0.409 ± 0.009	1.263HisAsp: 1.263 ± 0.017	1.324HisGlu: 1.324 ± 0.018	0.761HisPhe: 0.761 ± 0.011	1.746HisGly: 1.746 ± 0.02	0.986HisHis: 0.986 ± 0.018	0.804HisIle: 0.804 ± 0.012	1.05HisLys: 1.05 ± 0.015	1.792HisLeu: 1.792 ± 0.017	0.395HisMet: 0.395 ± 0.009	0.963HisAsn: 0.963 ± 0.014	1.444HisPro: 1.444 ± 0.019	1.073HisGln: 1.073 ± 0.021	1.764HisArg: 1.764 ± 0.02	1.82HisSer: 1.82 ± 0.021	1.082HisThr: 1.082 ± 0.016	1.218HisVal: 1.218 ± 0.014	0.275HisTrp: 0.275 ± 0.006	0.655HisTyr: 0.655 ± 0.012	0.06HisXaa: 0.06 ± 0.003
Ile	3.659IleAla: 3.659 ± 0.026	0.731IleCys: 0.731 ± 0.013	3.144IleAsp: 3.144 ± 0.027	3.244IleGlu: 3.244 ± 0.026	1.593IlePhe: 1.593 ± 0.019	3.062IleGly: 3.062 ± 0.026	1.041IleHis: 1.041 ± 0.014	1.762IleIle: 1.762 ± 0.019	1.944IleLys: 1.944 ± 0.018	4.077IleLeu: 4.077 ± 0.03	0.865IleMet: 0.865 ± 0.013	1.552IleAsn: 1.552 ± 0.017	2.556IlePro: 2.556 ± 0.025	1.718IleGln: 1.718 ± 0.019	2.645IleArg: 2.645 ± 0.022	3.35IleSer: 3.35 ± 0.027	2.103IleThr: 2.103 ± 0.02	3.025IleVal: 3.025 ± 0.025	0.43IleTrp: 0.43 ± 0.009	1.041IleTyr: 1.041 ± 0.014	0.1IleXaa: 0.1 ± 0.004
Lys	4.265LysAla: 4.265 ± 0.036	0.615LysCys: 0.615 ± 0.012	3.153LysAsp: 3.153 ± 0.025	4.334LysGlu: 4.334 ± 0.045	1.44LysPhe: 1.44 ± 0.016	3.222LysGly: 3.222 ± 0.034	1.251LysHis: 1.251 ± 0.014	2.579LysIle: 2.579 ± 0.027	5.249LysLys: 5.249 ± 0.054	4.103LysLeu: 4.103 ± 0.029	1.22LysMet: 1.22 ± 0.016	2.847LysAsn: 2.847 ± 0.025	2.518LysPro: 2.518 ± 0.025	2.338LysGln: 2.338 ± 0.024	3.927LysArg: 3.927 ± 0.035	4.285LysSer: 4.285 ± 0.038	3.343LysThr: 3.343 ± 0.03	2.663LysVal: 2.663 ± 0.023	0.527LysTrp: 0.527 ± 0.01	1.262LysTyr: 1.262 ± 0.016	0.172LysXaa: 0.172 ± 0.006
Leu	6.969LeuAla: 6.969 ± 0.04	1.405LeuCys: 1.405 ± 0.017	4.803LeuAsp: 4.803 ± 0.035	6.027LeuGlu: 6.027 ± 0.043	3.085LeuPhe: 3.085 ± 0.027	5.445LeuGly: 5.445 ± 0.042	1.858LeuHis: 1.858 ± 0.018	2.932LeuIle: 2.932 ± 0.022	4.116LeuLys: 4.116 ± 0.036	8.414LeuLeu: 8.414 ± 0.056	1.699LeuMet: 1.699 ± 0.018	2.759LeuAsn: 2.759 ± 0.027	4.77LeuPro: 4.77 ± 0.033	3.39LeuGln: 3.39 ± 0.029	5.412LeuArg: 5.412 ± 0.038	7.206LeuSer: 7.206 ± 0.04	3.866LeuThr: 3.866 ± 0.026	5.764LeuVal: 5.764 ± 0.038	1.043LeuTrp: 1.043 ± 0.014	2.027LeuTyr: 2.027 ± 0.02	0.187LeuXaa: 0.187 ± 0.005
Met	1.984MetAla: 1.984 ± 0.019	0.261MetCys: 0.261 ± 0.006	1.381MetAsp: 1.381 ± 0.017	1.656MetGlu: 1.656 ± 0.019	0.668MetPhe: 0.668 ± 0.014	1.516MetGly: 1.516 ± 0.016	0.421MetHis: 0.421 ± 0.008	1.034MetIle: 1.034 ± 0.013	1.394MetLys: 1.394 ± 0.017	1.64MetLeu: 1.64 ± 0.016	0.703MetMet: 0.703 ± 0.017	1.002MetAsn: 1.002 ± 0.015	1.028MetPro: 1.028 ± 0.014	0.772MetGln: 0.772 ± 0.011	1.114MetArg: 1.114 ± 0.014	1.617MetSer: 1.617 ± 0.018	1.272MetThr: 1.272 ± 0.015	1.48MetVal: 1.48 ± 0.015	0.191MetTrp: 0.191 ± 0.005	0.475MetTyr: 0.475 ± 0.009	0.05MetXaa: 0.05 ± 0.003
Asn	3.392AsnAla: 3.392 ± 0.026	0.596AsnCys: 0.596 ± 0.011	3.363AsnAsp: 3.363 ± 0.028	3.051AsnGlu: 3.051 ± 0.026	1.312AsnPhe: 1.312 ± 0.016	3.494AsnGly: 3.494 ± 0.028	1.224AsnHis: 1.224 ± 0.016	1.863AsnIle: 1.863 ± 0.017	2.417AsnLys: 2.417 ± 0.026	3.121AsnLeu: 3.121 ± 0.024	0.833AsnMet: 0.833 ± 0.012	3.093AsnAsn: 3.093 ± 0.041	2.362AsnPro: 2.362 ± 0.022	1.583AsnGln: 1.583 ± 0.018	2.533AsnArg: 2.533 ± 0.024	3.796AsnSer: 3.796 ± 0.037	2.44AsnThr: 2.44 ± 0.022	2.183AsnVal: 2.183 ± 0.02	0.393AsnTrp: 0.393 ± 0.008	1.067AsnTyr: 1.067 ± 0.016	0.161AsnXaa: 0.161 ± 0.006
Pro	3.875ProAla: 3.875 ± 0.042	0.65ProCys: 0.65 ± 0.011	2.634ProAsp: 2.634 ± 0.021	3.244ProGlu: 3.244 ± 0.029	1.776ProPhe: 1.776 ± 0.018	3.438ProGly: 3.438 ± 0.032	1.052ProHis: 1.052 ± 0.016	2.209ProIle: 2.209 ± 0.022	2.641ProLys: 2.641 ± 0.025	4.155ProLeu: 4.155 ± 0.028	1.01ProMet: 1.01 ± 0.014	1.997ProAsn: 1.997 ± 0.021	4.543ProPro: 4.543 ± 0.062	1.727ProGln: 1.727 ± 0.021	2.903ProArg: 2.903 ± 0.028	5.695ProSer: 5.695 ± 0.06	3.608ProThr: 3.608 ± 0.051	3.09ProVal: 3.09 ± 0.027	0.565ProTrp: 0.565 ± 0.01	1.123ProTyr: 1.123 ± 0.017	0.178ProXaa: 0.178 ± 0.006
Gln	2.547GlnAla: 2.547 ± 0.023	0.447GlnCys: 0.447 ± 0.009	1.689GlnAsp: 1.689 ± 0.016	2.515GlnGlu: 2.515 ± 0.028	0.984GlnPhe: 0.984 ± 0.012	1.912GlnGly: 1.912 ± 0.019	1.103GlnHis: 1.103 ± 0.018	1.574GlnIle: 1.574 ± 0.016	2.451GlnLys: 2.451 ± 0.022	2.905GlnLeu: 2.905 ± 0.026	0.804GlnMet: 0.804 ± 0.012	1.714GlnAsn: 1.714 ± 0.018	1.781GlnPro: 1.781 ± 0.025	3.664GlnGln: 3.664 ± 0.08	2.8GlnArg: 2.8 ± 0.028	2.842GlnSer: 2.842 ± 0.025	1.956GlnThr: 1.956 ± 0.019	1.703GlnVal: 1.703 ± 0.017	0.404GlnTrp: 0.404 ± 0.009	0.905GlnTyr: 0.905 ± 0.013	0.1GlnXaa: 0.1 ± 0.004
Arg	4.358ArgAla: 4.358 ± 0.037	1.008ArgCys: 1.008 ± 0.013	3.477ArgAsp: 3.477 ± 0.028	4.008ArgGlu: 4.008 ± 0.036	2.204ArgPhe: 2.204 ± 0.02	4.029ArgGly: 4.029 ± 0.038	1.483ArgHis: 1.483 ± 0.02	2.928ArgIle: 2.928 ± 0.023	4.177ArgLys: 4.177 ± 0.034	5.058ArgLeu: 5.058 ± 0.039	1.374ArgMet: 1.374 ± 0.016	2.974ArgAsn: 2.974 ± 0.026	2.758ArgPro: 2.758 ± 0.032	2.471ArgGln: 2.471 ± 0.021	5.467ArgArg: 5.467 ± 0.045	5.455ArgSer: 5.455 ± 0.035	3.291ArgThr: 3.291 ± 0.028	3.19ArgVal: 3.19 ± 0.023	0.769ArgTrp: 0.769 ± 0.011	1.512ArgTyr: 1.512 ± 0.017	0.162ArgXaa: 0.162 ± 0.005
Ser	6.374SerAla: 6.374 ± 0.039	1.273SerCys: 1.273 ± 0.017	5.063SerAsp: 5.063 ± 0.037	4.921SerGlu: 4.921 ± 0.033	3.326SerPhe: 3.326 ± 0.026	6.283SerGly: 6.283 ± 0.041	1.763SerHis: 1.763 ± 0.02	4.181SerIle: 4.181 ± 0.03	4.824SerLys: 4.824 ± 0.043	7.281SerLeu: 7.281 ± 0.041	1.967SerMet: 1.967 ± 0.021	4.48SerAsn: 4.48 ± 0.038	4.733SerPro: 4.733 ± 0.036	2.673SerGln: 2.673 ± 0.025	4.958SerArg: 4.958 ± 0.035	11.389SerSer: 11.389 ± 0.082	5.583SerThr: 5.583 ± 0.039	5.009SerVal: 5.009 ± 0.032	0.892SerTrp: 0.892 ± 0.015	1.916SerTyr: 1.916 ± 0.02	0.29SerXaa: 0.29 ± 0.008
Thr	4.945ThrAla: 4.945 ± 0.033	0.769ThrCys: 0.769 ± 0.013	3.133ThrAsp: 3.133 ± 0.025	3.628ThrGlu: 3.628 ± 0.029	1.76ThrPhe: 1.76 ± 0.018	4.047ThrGly: 4.047 ± 0.032	1.089ThrHis: 1.089 ± 0.014	2.969ThrIle: 2.969 ± 0.028	3.553ThrLys: 3.553 ± 0.026	4.314ThrLeu: 4.314 ± 0.028	1.303ThrMet: 1.303 ± 0.013	2.828ThrAsn: 2.828 ± 0.027	3.401ThrPro: 3.401 ± 0.033	1.69ThrGln: 1.69 ± 0.018	3.003ThrArg: 3.003 ± 0.026	5.122ThrSer: 5.122 ± 0.034	4.78ThrThr: 4.78 ± 0.036	3.441ThrVal: 3.441 ± 0.025	0.528ThrTrp: 0.528 ± 0.01	1.207ThrTyr: 1.207 ± 0.015	0.181ThrXaa: 0.181 ± 0.005
Val	5.448ValAla: 5.448 ± 0.036	1.017ValCys: 1.017 ± 0.013	3.865ValAsp: 3.865 ± 0.03	4.035ValGlu: 4.035 ± 0.032	2.263ValPhe: 2.263 ± 0.022	4.201ValGly: 4.201 ± 0.035	1.3ValHis: 1.3 ± 0.016	2.238ValIle: 2.238 ± 0.022	2.454ValLys: 2.454 ± 0.023	5.802ValLeu: 5.802 ± 0.041	1.198ValMet: 1.198 ± 0.015	1.916ValAsn: 1.916 ± 0.018	3.358ValPro: 3.358 ± 0.024	1.926ValGln: 1.926 ± 0.019	3.411ValArg: 3.411 ± 0.026	4.785ValSer: 4.785 ± 0.031	3.122ValThr: 3.122 ± 0.025	4.911ValVal: 4.911 ± 0.037	0.698ValTrp: 0.698 ± 0.012	1.421ValTyr: 1.421 ± 0.018	0.131ValXaa: 0.131 ± 0.004
Trp	0.696TrpAla: 0.696 ± 0.011	0.207TrpCys: 0.207 ± 0.006	0.716TrpAsp: 0.716 ± 0.013	0.719TrpGlu: 0.719 ± 0.013	0.451TrpPhe: 0.451 ± 0.01	0.674TrpGly: 0.674 ± 0.011	0.241TrpHis: 0.241 ± 0.007	0.649TrpIle: 0.649 ± 0.012	0.804TrpLys: 0.804 ± 0.012	0.942TrpLeu: 0.942 ± 0.015	0.36TrpMet: 0.36 ± 0.008	0.595TrpAsn: 0.595 ± 0.01	0.389TrpPro: 0.389 ± 0.008	0.369TrpGln: 0.369 ± 0.008	0.682TrpArg: 0.682 ± 0.012	0.847TrpSer: 0.847 ± 0.012	0.671TrpThr: 0.671 ± 0.011	0.585TrpVal: 0.585 ± 0.009	0.203TrpTrp: 0.203 ± 0.007	0.332TrpTyr: 0.332 ± 0.008	0.022TrpXaa: 0.022 ± 0.002
Tyr	1.637TyrAla: 1.637 ± 0.017	0.458TyrCys: 0.458 ± 0.009	1.751TyrAsp: 1.751 ± 0.017	1.599TyrGlu: 1.599 ± 0.016	0.978TyrPhe: 0.978 ± 0.015	1.891TyrGly: 1.891 ± 0.022	0.684TyrHis: 0.684 ± 0.011	0.925TyrIle: 0.925 ± 0.012	1.109TyrLys: 1.109 ± 0.014	2.086TyrLeu: 2.086 ± 0.021	0.493TyrMet: 0.493 ± 0.011	1.109TyrAsn: 1.109 ± 0.017	1.084TyrPro: 1.084 ± 0.017	0.946TyrGln: 0.946 ± 0.015	1.507TyrArg: 1.507 ± 0.017	1.841TyrSer: 1.841 ± 0.022	1.19TyrThr: 1.19 ± 0.015	1.385TyrVal: 1.385 ± 0.015	0.325TyrTrp: 0.325 ± 0.008	0.885TyrTyr: 0.885 ± 0.019	0.049TyrXaa: 0.049 ± 0.003
Xaa	0.214XaaAla: 0.214 ± 0.007	0.038XaaCys: 0.038 ± 0.003	0.198XaaAsp: 0.198 ± 0.006	0.21XaaGlu: 0.21 ± 0.006	0.079XaaPhe: 0.079 ± 0.004	0.225XaaGly: 0.225 ± 0.007	0.063XaaHis: 0.063 ± 0.003	0.111XaaIle: 0.111 ± 0.004	0.175XaaLys: 0.175 ± 0.006	0.193XaaLeu: 0.193 ± 0.006	0.053XaaMet: 0.053 ± 0.003	0.147XaaAsn: 0.147 ± 0.005	0.146XaaPro: 0.146 ± 0.006	0.096XaaGln: 0.096 ± 0.004	0.169XaaArg: 0.169 ± 0.006	0.282XaaSer: 0.282 ± 0.009	0.169XaaThr: 0.169 ± 0.005	0.153XaaVal: 0.153 ± 0.005	0.025XaaTrp: 0.025 ± 0.002	0.049XaaTyr: 0.049 ± 0.003	0.414XaaXaa: 0.414 ± 0.057

Statistics based on 11907 proteins (6325991 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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