Amino acid dipepetide frequency for Flavobacterium sp. 9AF

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.296AlaAla: 3.296 ± 0.072	0.498AlaCys: 0.498 ± 0.026	2.58AlaAsp: 2.58 ± 0.05	3.326AlaGlu: 3.326 ± 0.059	3.096AlaPhe: 3.096 ± 0.048	3.466AlaGly: 3.466 ± 0.067	0.912AlaHis: 0.912 ± 0.03	5.384AlaIle: 5.384 ± 0.077	4.548AlaLys: 4.548 ± 0.077	5.552AlaLeu: 5.552 ± 0.077	1.208AlaMet: 1.208 ± 0.04	3.735AlaAsn: 3.735 ± 0.059	1.648AlaPro: 1.648 ± 0.042	2.204AlaGln: 2.204 ± 0.047	1.552AlaArg: 1.552 ± 0.041	3.946AlaSer: 3.946 ± 0.059	4.01AlaThr: 4.01 ± 0.085	3.471AlaVal: 3.471 ± 0.064	0.517AlaTrp: 0.517 ± 0.022	2.266AlaTyr: 2.266 ± 0.04	0.0AlaXaa: 0.0 ± 0.0
Cys	0.409CysAla: 0.409 ± 0.019	0.105CysCys: 0.105 ± 0.01	0.424CysAsp: 0.424 ± 0.019	0.492CysGlu: 0.492 ± 0.023	0.464CysPhe: 0.464 ± 0.021	0.575CysGly: 0.575 ± 0.027	0.189CysHis: 0.189 ± 0.014	0.63CysIle: 0.63 ± 0.023	0.572CysLys: 0.572 ± 0.022	0.698CysLeu: 0.698 ± 0.026	0.131CysMet: 0.131 ± 0.011	0.549CysAsn: 0.549 ± 0.024	0.333CysPro: 0.333 ± 0.02	0.262CysGln: 0.262 ± 0.014	0.183CysArg: 0.183 ± 0.014	0.61CysSer: 0.61 ± 0.025	0.498CysThr: 0.498 ± 0.03	0.448CysVal: 0.448 ± 0.02	0.071CysTrp: 0.071 ± 0.007	0.354CysTyr: 0.354 ± 0.017	0.0CysXaa: 0.0 ± 0.0
Asp	3.204AspAla: 3.204 ± 0.056	0.439AspCys: 0.439 ± 0.019	2.484AspAsp: 2.484 ± 0.056	3.441AspGlu: 3.441 ± 0.052	3.8AspPhe: 3.8 ± 0.058	3.139AspGly: 3.139 ± 0.066	0.66AspHis: 0.66 ± 0.025	4.267AspIle: 4.267 ± 0.065	4.111AspLys: 4.111 ± 0.075	4.927AspLeu: 4.927 ± 0.067	0.876AspMet: 0.876 ± 0.03	3.399AspAsn: 3.399 ± 0.058	1.368AspPro: 1.368 ± 0.039	1.315AspGln: 1.315 ± 0.031	1.522AspArg: 1.522 ± 0.05	3.094AspSer: 3.094 ± 0.064	2.686AspThr: 2.686 ± 0.044	3.047AspVal: 3.047 ± 0.054	0.692AspTrp: 0.692 ± 0.025	2.853AspTyr: 2.853 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	3.884GluAla: 3.884 ± 0.071	0.372GluCys: 0.372 ± 0.017	3.305GluAsp: 3.305 ± 0.063	5.007GluGlu: 5.007 ± 0.086	2.839GluPhe: 2.839 ± 0.05	3.384GluGly: 3.384 ± 0.062	0.962GluHis: 0.962 ± 0.031	6.49GluIle: 6.49 ± 0.08	7.061GluLys: 7.061 ± 0.101	5.832GluLeu: 5.832 ± 0.089	1.648GluMet: 1.648 ± 0.034	5.713GluAsn: 5.713 ± 0.069	1.499GluPro: 1.499 ± 0.034	2.138GluGln: 2.138 ± 0.053	2.154GluArg: 2.154 ± 0.048	3.216GluSer: 3.216 ± 0.053	3.71GluThr: 3.71 ± 0.063	3.99GluVal: 3.99 ± 0.064	0.634GluTrp: 0.634 ± 0.022	2.599GluTyr: 2.599 ± 0.043	0.0GluXaa: 0.0 ± 0.0
Phe	2.81PheAla: 2.81 ± 0.055	0.501PheCys: 0.501 ± 0.022	3.228PheAsp: 3.228 ± 0.049	3.767PheGlu: 3.767 ± 0.055	3.16PhePhe: 3.16 ± 0.067	3.388PheGly: 3.388 ± 0.058	0.884PheHis: 0.884 ± 0.03	4.551PheIle: 4.551 ± 0.072	3.977PheLys: 3.977 ± 0.061	5.246PheLeu: 5.246 ± 0.089	1.035PheMet: 1.035 ± 0.028	3.673PheAsn: 3.673 ± 0.061	1.79PhePro: 1.79 ± 0.041	1.815PheGln: 1.815 ± 0.036	1.442PheArg: 1.442 ± 0.038	4.483PheSer: 4.483 ± 0.059	3.428PheThr: 3.428 ± 0.069	3.009PheVal: 3.009 ± 0.053	0.567PheTrp: 0.567 ± 0.022	2.631PheTyr: 2.631 ± 0.048	0.0PheXaa: 0.0 ± 0.0
Gly	3.507GlyAla: 3.507 ± 0.067	0.587GlyCys: 0.587 ± 0.031	2.708GlyAsp: 2.708 ± 0.052	3.039GlyGlu: 3.039 ± 0.057	3.442GlyPhe: 3.442 ± 0.066	4.07GlyGly: 4.07 ± 0.084	0.983GlyHis: 0.983 ± 0.03	5.341GlyIle: 5.341 ± 0.08	4.848GlyLys: 4.848 ± 0.08	5.259GlyLeu: 5.259 ± 0.062	1.283GlyMet: 1.283 ± 0.036	4.051GlyAsn: 4.051 ± 0.082	1.179GlyPro: 1.179 ± 0.037	1.782GlyGln: 1.782 ± 0.04	1.681GlyArg: 1.681 ± 0.039	3.864GlySer: 3.864 ± 0.077	4.161GlyThr: 4.161 ± 0.111	3.753GlyVal: 3.753 ± 0.065	0.678GlyTrp: 0.678 ± 0.026	2.827GlyTyr: 2.827 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	0.812HisAla: 0.812 ± 0.029	0.189HisCys: 0.189 ± 0.014	0.782HisAsp: 0.782 ± 0.026	0.94HisGlu: 0.94 ± 0.028	1.277HisPhe: 1.277 ± 0.031	0.856HisGly: 0.856 ± 0.028	0.423HisHis: 0.423 ± 0.021	1.333HisIle: 1.333 ± 0.036	1.126HisLys: 1.126 ± 0.03	1.735HisLeu: 1.735 ± 0.038	0.24HisMet: 0.24 ± 0.014	0.951HisAsn: 0.951 ± 0.027	0.744HisPro: 0.744 ± 0.03	0.71HisGln: 0.71 ± 0.028	0.519HisArg: 0.519 ± 0.02	1.089HisSer: 1.089 ± 0.026	0.89HisThr: 0.89 ± 0.028	0.821HisVal: 0.821 ± 0.026	0.226HisTrp: 0.226 ± 0.014	0.846HisTyr: 0.846 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	5.442IleAla: 5.442 ± 0.072	0.71IleCys: 0.71 ± 0.024	5.09IleAsp: 5.09 ± 0.072	6.138IleGlu: 6.138 ± 0.082	3.969IlePhe: 3.969 ± 0.07	5.162IleGly: 5.162 ± 0.072	1.478IleHis: 1.478 ± 0.038	7.138IleIle: 7.138 ± 0.092	6.633IleLys: 6.633 ± 0.098	7.806IleLeu: 7.806 ± 0.092	1.417IleMet: 1.417 ± 0.038	5.629IleAsn: 5.629 ± 0.075	3.28IlePro: 3.28 ± 0.049	3.219IleGln: 3.219 ± 0.054	2.355IleArg: 2.355 ± 0.043	6.07IleSer: 6.07 ± 0.075	5.477IleThr: 5.477 ± 0.085	5.063IleVal: 5.063 ± 0.07	0.74IleTrp: 0.74 ± 0.027	3.374IleTyr: 3.374 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	4.72LysAla: 4.72 ± 0.073	0.401LysCys: 0.401 ± 0.017	4.363LysAsp: 4.363 ± 0.068	7.184LysGlu: 7.184 ± 0.101	3.088LysPhe: 3.088 ± 0.052	4.33LysGly: 4.33 ± 0.067	1.253LysHis: 1.253 ± 0.036	7.887LysIle: 7.887 ± 0.101	8.119LysLys: 8.119 ± 0.113	6.586LysLeu: 6.586 ± 0.082	2.258LysMet: 2.258 ± 0.044	6.52LysAsn: 6.52 ± 0.091	2.376LysPro: 2.376 ± 0.045	2.8LysGln: 2.8 ± 0.048	2.633LysArg: 2.633 ± 0.052	4.541LysSer: 4.541 ± 0.067	4.803LysThr: 4.803 ± 0.074	4.52LysVal: 4.52 ± 0.068	0.81LysTrp: 0.81 ± 0.025	3.353LysTyr: 3.353 ± 0.057	0.0LysXaa: 0.0 ± 0.0
Leu	5.255LeuAla: 5.255 ± 0.084	0.659LeuCys: 0.659 ± 0.025	4.876LeuAsp: 4.876 ± 0.068	6.233LeuGlu: 6.233 ± 0.086	5.546LeuPhe: 5.546 ± 0.09	5.42LeuGly: 5.42 ± 0.072	1.585LeuHis: 1.585 ± 0.033	7.196LeuIle: 7.196 ± 0.099	7.81LeuLys: 7.81 ± 0.113	8.984LeuLeu: 8.984 ± 0.124	1.746LeuMet: 1.746 ± 0.042	6.158LeuAsn: 6.158 ± 0.075	3.405LeuPro: 3.405 ± 0.052	3.557LeuGln: 3.557 ± 0.062	2.668LeuArg: 2.668 ± 0.054	6.605LeuSer: 6.605 ± 0.078	5.05LeuThr: 5.05 ± 0.071	5.053LeuVal: 5.053 ± 0.079	0.773LeuTrp: 0.773 ± 0.028	3.449LeuTyr: 3.449 ± 0.065	0.0LeuXaa: 0.0 ± 0.0
Met	1.31MetAla: 1.31 ± 0.036	0.138MetCys: 0.138 ± 0.011	0.954MetAsp: 0.954 ± 0.027	1.318MetGlu: 1.318 ± 0.031	0.796MetPhe: 0.796 ± 0.026	1.174MetGly: 1.174 ± 0.03	0.389MetHis: 0.389 ± 0.016	1.543MetIle: 1.543 ± 0.034	2.279MetLys: 2.279 ± 0.039	1.731MetLeu: 1.731 ± 0.045	0.493MetMet: 0.493 ± 0.022	1.403MetAsn: 1.403 ± 0.033	0.682MetPro: 0.682 ± 0.024	0.785MetGln: 0.785 ± 0.027	0.707MetArg: 0.707 ± 0.022	1.282MetSer: 1.282 ± 0.035	1.036MetThr: 1.036 ± 0.028	1.141MetVal: 1.141 ± 0.031	0.144MetTrp: 0.144 ± 0.012	0.762MetTyr: 0.762 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	4.116AsnAla: 4.116 ± 0.057	0.641AsnCys: 0.641 ± 0.027	3.648AsnAsp: 3.648 ± 0.058	4.793AsnGlu: 4.793 ± 0.065	3.808AsnPhe: 3.808 ± 0.057	4.62AsnGly: 4.62 ± 0.093	1.226AsnHis: 1.226 ± 0.033	5.363AsnIle: 5.363 ± 0.08	5.317AsnLys: 5.317 ± 0.08	6.205AsnLeu: 6.205 ± 0.076	1.26AsnMet: 1.26 ± 0.028	5.245AsnAsn: 5.245 ± 0.101	3.075AsnPro: 3.075 ± 0.06	2.934AsnGln: 2.934 ± 0.05	2.11AsnArg: 2.11 ± 0.047	4.592AsnSer: 4.592 ± 0.078	4.285AsnThr: 4.285 ± 0.083	3.717AsnVal: 3.717 ± 0.072	0.928AsnTrp: 0.928 ± 0.03	3.65AsnTyr: 3.65 ± 0.064	0.0AsnXaa: 0.0 ± 0.0
Pro	1.651ProAla: 1.651 ± 0.045	0.219ProCys: 0.219 ± 0.015	1.603ProAsp: 1.603 ± 0.036	2.342ProGlu: 2.342 ± 0.05	1.935ProPhe: 1.935 ± 0.037	1.538ProGly: 1.538 ± 0.045	0.494ProHis: 0.494 ± 0.023	2.867ProIle: 2.867 ± 0.047	2.523ProLys: 2.523 ± 0.047	2.84ProLeu: 2.84 ± 0.047	0.626ProMet: 0.626 ± 0.021	2.66ProAsn: 2.66 ± 0.057	0.7ProPro: 0.7 ± 0.033	1.032ProGln: 1.032 ± 0.029	0.76ProArg: 0.76 ± 0.024	2.24ProSer: 2.24 ± 0.047	2.069ProThr: 2.069 ± 0.052	2.098ProVal: 2.098 ± 0.044	0.285ProTrp: 0.285 ± 0.015	1.415ProTyr: 1.415 ± 0.032	0.0ProXaa: 0.0 ± 0.0
Gln	1.886GlnAla: 1.886 ± 0.045	0.216GlnCys: 0.216 ± 0.012	1.632GlnAsp: 1.632 ± 0.038	2.475GlnGlu: 2.475 ± 0.048	1.884GlnPhe: 1.884 ± 0.045	1.675GlnGly: 1.675 ± 0.04	0.56GlnHis: 0.56 ± 0.02	3.077GlnIle: 3.077 ± 0.046	3.296GlnLys: 3.296 ± 0.058	3.602GlnLeu: 3.602 ± 0.062	0.845GlnMet: 0.845 ± 0.026	2.804GlnAsn: 2.804 ± 0.051	1.096GlnPro: 1.096 ± 0.029	1.342GlnGln: 1.342 ± 0.041	0.969GlnArg: 0.969 ± 0.028	2.015GlnSer: 2.015 ± 0.04	1.975GlnThr: 1.975 ± 0.041	1.857GlnVal: 1.857 ± 0.04	0.362GlnTrp: 0.362 ± 0.018	1.486GlnTyr: 1.486 ± 0.038	0.0GlnXaa: 0.0 ± 0.0
Arg	1.647ArgAla: 1.647 ± 0.043	0.182ArgCys: 0.182 ± 0.011	1.369ArgAsp: 1.369 ± 0.033	1.864ArgGlu: 1.864 ± 0.045	1.72ArgPhe: 1.72 ± 0.04	1.493ArgGly: 1.493 ± 0.036	0.439ArgHis: 0.439 ± 0.021	2.765ArgIle: 2.765 ± 0.057	2.536ArgLys: 2.536 ± 0.048	2.806ArgLeu: 2.806 ± 0.06	0.809ArgMet: 0.809 ± 0.023	2.125ArgAsn: 2.125 ± 0.042	0.836ArgPro: 0.836 ± 0.028	0.857ArgGln: 0.857 ± 0.023	0.996ArgArg: 0.996 ± 0.032	1.51ArgSer: 1.51 ± 0.033	1.537ArgThr: 1.537 ± 0.034	1.788ArgVal: 1.788 ± 0.04	0.316ArgTrp: 0.316 ± 0.018	1.248ArgTyr: 1.248 ± 0.031	0.0ArgXaa: 0.0 ± 0.0
Ser	3.149SerAla: 3.149 ± 0.06	0.739SerCys: 0.739 ± 0.027	3.227SerAsp: 3.227 ± 0.046	3.923SerGlu: 3.923 ± 0.056	4.284SerPhe: 4.284 ± 0.062	4.471SerGly: 4.471 ± 0.101	1.098SerHis: 1.098 ± 0.034	5.752SerIle: 5.752 ± 0.074	5.229SerLys: 5.229 ± 0.066	6.178SerLeu: 6.178 ± 0.076	1.164SerMet: 1.164 ± 0.031	4.814SerAsn: 4.814 ± 0.086	1.908SerPro: 1.908 ± 0.043	2.37SerGln: 2.37 ± 0.047	1.789SerArg: 1.789 ± 0.041	4.631SerSer: 4.631 ± 0.081	3.64SerThr: 3.64 ± 0.084	3.701SerVal: 3.701 ± 0.055	0.702SerTrp: 0.702 ± 0.025	2.971SerTyr: 2.971 ± 0.058	0.0SerXaa: 0.0 ± 0.0
Thr	3.452ThrAla: 3.452 ± 0.065	0.429ThrCys: 0.429 ± 0.026	3.012ThrAsp: 3.012 ± 0.052	3.349ThrGlu: 3.349 ± 0.059	3.428ThrPhe: 3.428 ± 0.053	3.854ThrGly: 3.854 ± 0.088	1.069ThrHis: 1.069 ± 0.031	5.751ThrIle: 5.751 ± 0.073	4.172ThrLys: 4.172 ± 0.059	5.526ThrLeu: 5.526 ± 0.067	0.892ThrMet: 0.892 ± 0.026	4.427ThrAsn: 4.427 ± 0.091	2.365ThrPro: 2.365 ± 0.06	2.002ThrGln: 2.002 ± 0.043	1.383ThrArg: 1.383 ± 0.038	4.156ThrSer: 4.156 ± 0.074	4.285ThrThr: 4.285 ± 0.106	3.705ThrVal: 3.705 ± 0.08	0.585ThrTrp: 0.585 ± 0.024	2.58ThrTyr: 2.58 ± 0.069	0.0ThrXaa: 0.0 ± 0.0
Val	3.781ValAla: 3.781 ± 0.059	0.496ValCys: 0.496 ± 0.022	3.072ValAsp: 3.072 ± 0.052	3.529ValGlu: 3.529 ± 0.061	3.353ValPhe: 3.353 ± 0.053	3.3ValGly: 3.3 ± 0.055	0.876ValHis: 0.876 ± 0.028	4.835ValIle: 4.835 ± 0.071	4.197ValLys: 4.197 ± 0.068	5.51ValLeu: 5.51 ± 0.083	1.168ValMet: 1.168 ± 0.033	3.655ValAsn: 3.655 ± 0.058	1.939ValPro: 1.939 ± 0.041	1.762ValGln: 1.762 ± 0.037	1.704ValArg: 1.704 ± 0.04	4.198ValSer: 4.198 ± 0.071	3.777ValThr: 3.777 ± 0.081	3.799ValVal: 3.799 ± 0.071	0.532ValTrp: 0.532 ± 0.025	2.357ValTyr: 2.357 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.519TrpAla: 0.519 ± 0.023	0.103TrpCys: 0.103 ± 0.011	0.543TrpAsp: 0.543 ± 0.021	0.678TrpGlu: 0.678 ± 0.027	0.602TrpPhe: 0.602 ± 0.024	0.587TrpGly: 0.587 ± 0.024	0.216TrpHis: 0.216 ± 0.012	0.821TrpIle: 0.821 ± 0.027	0.859TrpLys: 0.859 ± 0.029	0.873TrpLeu: 0.873 ± 0.028	0.267TrpMet: 0.267 ± 0.016	0.817TrpAsn: 0.817 ± 0.027	0.192TrpPro: 0.192 ± 0.015	0.45TrpGln: 0.45 ± 0.02	0.318TrpArg: 0.318 ± 0.015	0.645TrpSer: 0.645 ± 0.024	0.559TrpThr: 0.559 ± 0.024	0.568TrpVal: 0.568 ± 0.023	0.132TrpTrp: 0.132 ± 0.013	0.457TrpTyr: 0.457 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.365TyrAla: 2.365 ± 0.045	0.37TyrCys: 0.37 ± 0.018	2.423TyrAsp: 2.423 ± 0.053	2.6TyrGlu: 2.6 ± 0.049	2.893TyrPhe: 2.893 ± 0.051	2.538TyrGly: 2.538 ± 0.056	0.814TyrHis: 0.814 ± 0.03	3.2TyrIle: 3.2 ± 0.05	3.334TyrLys: 3.334 ± 0.057	4.132TyrLeu: 4.132 ± 0.064	0.699TyrMet: 0.699 ± 0.022	3.208TyrAsn: 3.208 ± 0.067	1.481TyrPro: 1.481 ± 0.034	1.718TyrGln: 1.718 ± 0.04	1.397TyrArg: 1.397 ± 0.034	2.952TyrSer: 2.952 ± 0.059	2.546TyrThr: 2.546 ± 0.055	2.292TyrVal: 2.292 ± 0.046	0.514TyrTrp: 0.514 ± 0.02	2.243TyrTyr: 2.243 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3815 proteins (1261417 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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