Amino acid dipepetide frequency for Polaribacter sp. KT25b

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.904AlaAla: 3.904 ± 0.079	0.468AlaCys: 0.468 ± 0.021	3.383AlaAsp: 3.383 ± 0.067	3.481AlaGlu: 3.481 ± 0.059	3.231AlaPhe: 3.231 ± 0.061	3.638AlaGly: 3.638 ± 0.08	0.945AlaHis: 0.945 ± 0.033	5.469AlaIle: 5.469 ± 0.092	4.894AlaLys: 4.894 ± 0.083	5.372AlaLeu: 5.372 ± 0.079	1.245AlaMet: 1.245 ± 0.035	3.546AlaAsn: 3.546 ± 0.061	1.545AlaPro: 1.545 ± 0.031	2.044AlaGln: 2.044 ± 0.045	1.697AlaArg: 1.697 ± 0.047	4.005AlaSer: 4.005 ± 0.061	3.838AlaThr: 3.838 ± 0.07	3.637AlaVal: 3.637 ± 0.065	0.532AlaTrp: 0.532 ± 0.025	2.12AlaTyr: 2.12 ± 0.044	0.0AlaXaa: 0.0 ± 0.0
Cys	0.446CysAla: 0.446 ± 0.026	0.085CysCys: 0.085 ± 0.009	0.382CysAsp: 0.382 ± 0.021	0.386CysGlu: 0.386 ± 0.018	0.411CysPhe: 0.411 ± 0.022	0.528CysGly: 0.528 ± 0.027	0.157CysHis: 0.157 ± 0.014	0.542CysIle: 0.542 ± 0.022	0.54CysLys: 0.54 ± 0.024	0.563CysLeu: 0.563 ± 0.017	0.127CysMet: 0.127 ± 0.011	0.411CysAsn: 0.411 ± 0.019	0.254CysPro: 0.254 ± 0.016	0.162CysGln: 0.162 ± 0.013	0.156CysArg: 0.156 ± 0.012	0.526CysSer: 0.526 ± 0.03	0.396CysThr: 0.396 ± 0.021	0.375CysVal: 0.375 ± 0.02	0.055CysTrp: 0.055 ± 0.006	0.273CysTyr: 0.273 ± 0.016	0.0CysXaa: 0.0 ± 0.0
Asp	3.75AspAla: 3.75 ± 0.057	0.386AspCys: 0.386 ± 0.019	3.0AspAsp: 3.0 ± 0.067	3.992AspGlu: 3.992 ± 0.066	3.889AspPhe: 3.889 ± 0.066	3.386AspGly: 3.386 ± 0.058	0.704AspHis: 0.704 ± 0.025	4.516AspIle: 4.516 ± 0.069	4.725AspLys: 4.725 ± 0.067	5.286AspLeu: 5.286 ± 0.077	0.89AspMet: 0.89 ± 0.026	3.322AspAsn: 3.322 ± 0.051	1.339AspPro: 1.339 ± 0.04	1.255AspGln: 1.255 ± 0.04	1.564AspArg: 1.564 ± 0.036	3.055AspSer: 3.055 ± 0.05	2.7AspThr: 2.7 ± 0.051	3.814AspVal: 3.814 ± 0.068	0.756AspTrp: 0.756 ± 0.03	2.696AspTyr: 2.696 ± 0.049	0.0AspXaa: 0.0 ± 0.0
Glu	3.863GluAla: 3.863 ± 0.066	0.304GluCys: 0.304 ± 0.017	3.479GluAsp: 3.479 ± 0.055	4.684GluGlu: 4.684 ± 0.078	3.174GluPhe: 3.174 ± 0.062	3.459GluGly: 3.459 ± 0.067	0.986GluHis: 0.986 ± 0.028	6.399GluIle: 6.399 ± 0.089	6.654GluLys: 6.654 ± 0.087	6.052GluLeu: 6.052 ± 0.08	1.516GluMet: 1.516 ± 0.04	5.635GluAsn: 5.635 ± 0.086	1.332GluPro: 1.332 ± 0.037	1.984GluGln: 1.984 ± 0.041	2.174GluArg: 2.174 ± 0.048	3.417GluSer: 3.417 ± 0.059	3.835GluThr: 3.835 ± 0.065	4.034GluVal: 4.034 ± 0.067	0.578GluTrp: 0.578 ± 0.024	2.462GluTyr: 2.462 ± 0.045	0.0GluXaa: 0.0 ± 0.0
Phe	2.926PheAla: 2.926 ± 0.05	0.446PheCys: 0.446 ± 0.021	3.368PheAsp: 3.368 ± 0.055	3.289PheGlu: 3.289 ± 0.057	3.079PhePhe: 3.079 ± 0.069	3.699PheGly: 3.699 ± 0.067	0.809PheHis: 0.809 ± 0.026	4.486PheIle: 4.486 ± 0.079	4.421PheLys: 4.421 ± 0.059	5.206PheLeu: 5.206 ± 0.088	1.106PheMet: 1.106 ± 0.032	3.742PheAsn: 3.742 ± 0.067	1.636PhePro: 1.636 ± 0.041	1.389PheGln: 1.389 ± 0.034	1.497PheArg: 1.497 ± 0.039	4.555PheSer: 4.555 ± 0.078	3.544PheThr: 3.544 ± 0.058	3.104PheVal: 3.104 ± 0.064	0.58PheTrp: 0.58 ± 0.023	2.44PheTyr: 2.44 ± 0.04	0.0PheXaa: 0.0 ± 0.0
Gly	3.802GlyAla: 3.802 ± 0.073	0.452GlyCys: 0.452 ± 0.022	3.117GlyAsp: 3.117 ± 0.059	3.35GlyGlu: 3.35 ± 0.056	3.79GlyPhe: 3.79 ± 0.061	4.17GlyGly: 4.17 ± 0.089	0.976GlyHis: 0.976 ± 0.031	5.248GlyIle: 5.248 ± 0.084	5.17GlyLys: 5.17 ± 0.075	5.357GlyLeu: 5.357 ± 0.074	1.357GlyMet: 1.357 ± 0.041	3.781GlyAsn: 3.781 ± 0.059	1.12GlyPro: 1.12 ± 0.035	1.54GlyGln: 1.54 ± 0.035	1.795GlyArg: 1.795 ± 0.046	3.749GlySer: 3.749 ± 0.06	3.743GlyThr: 3.743 ± 0.072	4.165GlyVal: 4.165 ± 0.065	0.679GlyTrp: 0.679 ± 0.026	2.592GlyTyr: 2.592 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	0.8HisAla: 0.8 ± 0.03	0.142HisCys: 0.142 ± 0.01	0.727HisAsp: 0.727 ± 0.032	0.843HisGlu: 0.843 ± 0.03	1.077HisPhe: 1.077 ± 0.031	0.9HisGly: 0.9 ± 0.03	0.429HisHis: 0.429 ± 0.023	1.338HisIle: 1.338 ± 0.036	1.308HisLys: 1.308 ± 0.037	1.679HisLeu: 1.679 ± 0.04	0.263HisMet: 0.263 ± 0.015	0.96HisAsn: 0.96 ± 0.028	0.791HisPro: 0.791 ± 0.028	0.706HisGln: 0.706 ± 0.024	0.564HisArg: 0.564 ± 0.022	0.925HisSer: 0.925 ± 0.03	0.895HisThr: 0.895 ± 0.03	0.786HisVal: 0.786 ± 0.026	0.182HisTrp: 0.182 ± 0.011	0.708HisTyr: 0.708 ± 0.026	0.0HisXaa: 0.0 ± 0.0
Ile	5.494IleAla: 5.494 ± 0.088	0.688IleCys: 0.688 ± 0.028	5.333IleAsp: 5.333 ± 0.083	5.81IleGlu: 5.81 ± 0.079	4.105IlePhe: 4.105 ± 0.077	5.094IleGly: 5.094 ± 0.084	1.368IleHis: 1.368 ± 0.035	7.221IleIle: 7.221 ± 0.113	7.252IleLys: 7.252 ± 0.091	7.509IleLeu: 7.509 ± 0.096	1.353IleMet: 1.353 ± 0.036	5.665IleAsn: 5.665 ± 0.087	3.233IlePro: 3.233 ± 0.056	2.511IleGln: 2.511 ± 0.054	2.407IleArg: 2.407 ± 0.044	6.527IleSer: 6.527 ± 0.087	5.341IleThr: 5.341 ± 0.073	4.982IleVal: 4.982 ± 0.068	0.723IleTrp: 0.723 ± 0.025	3.048IleTyr: 3.048 ± 0.058	0.0IleXaa: 0.0 ± 0.0
Lys	4.798LysAla: 4.798 ± 0.075	0.324LysCys: 0.324 ± 0.018	4.815LysAsp: 4.815 ± 0.073	7.614LysGlu: 7.614 ± 0.106	3.435LysPhe: 3.435 ± 0.065	4.904LysGly: 4.904 ± 0.082	1.428LysHis: 1.428 ± 0.037	7.847LysIle: 7.847 ± 0.094	8.891LysLys: 8.891 ± 0.116	7.224LysLeu: 7.224 ± 0.095	2.18LysMet: 2.18 ± 0.046	7.036LysAsn: 7.036 ± 0.094	2.542LysPro: 2.542 ± 0.053	2.822LysGln: 2.822 ± 0.057	2.84LysArg: 2.84 ± 0.056	5.177LysSer: 5.177 ± 0.073	5.373LysThr: 5.373 ± 0.078	5.092LysVal: 5.092 ± 0.068	0.867LysTrp: 0.867 ± 0.027	3.427LysTyr: 3.427 ± 0.07	0.0LysXaa: 0.0 ± 0.0
Leu	5.31LeuAla: 5.31 ± 0.085	0.549LeuCys: 0.549 ± 0.02	4.971LeuAsp: 4.971 ± 0.063	6.478LeuGlu: 6.478 ± 0.094	5.141LeuPhe: 5.141 ± 0.082	5.581LeuGly: 5.581 ± 0.08	1.418LeuHis: 1.418 ± 0.039	7.435LeuIle: 7.435 ± 0.097	8.588LeuLys: 8.588 ± 0.103	8.446LeuLeu: 8.446 ± 0.118	1.838LeuMet: 1.838 ± 0.043	6.059LeuAsn: 6.059 ± 0.081	3.29LeuPro: 3.29 ± 0.053	3.092LeuGln: 3.092 ± 0.044	2.729LeuArg: 2.729 ± 0.051	6.72LeuSer: 6.72 ± 0.09	5.138LeuThr: 5.138 ± 0.092	5.049LeuVal: 5.049 ± 0.074	0.735LeuTrp: 0.735 ± 0.029	3.102LeuTyr: 3.102 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	1.318MetAla: 1.318 ± 0.038	0.125MetCys: 0.125 ± 0.012	0.972MetAsp: 0.972 ± 0.031	1.104MetGlu: 1.104 ± 0.03	0.907MetPhe: 0.907 ± 0.03	1.17MetGly: 1.17 ± 0.037	0.369MetHis: 0.369 ± 0.02	1.526MetIle: 1.526 ± 0.037	2.275MetLys: 2.275 ± 0.045	1.814MetLeu: 1.814 ± 0.041	0.54MetMet: 0.54 ± 0.024	1.269MetAsn: 1.269 ± 0.034	0.736MetPro: 0.736 ± 0.031	0.712MetGln: 0.712 ± 0.027	0.719MetArg: 0.719 ± 0.024	1.321MetSer: 1.321 ± 0.034	0.98MetThr: 0.98 ± 0.031	1.133MetVal: 1.133 ± 0.034	0.157MetTrp: 0.157 ± 0.013	0.699MetTyr: 0.699 ± 0.024	0.0MetXaa: 0.0 ± 0.0
Asn	3.805AsnAla: 3.805 ± 0.057	0.472AsnCys: 0.472 ± 0.031	3.363AsnAsp: 3.363 ± 0.057	3.989AsnGlu: 3.989 ± 0.066	3.657AsnPhe: 3.657 ± 0.069	4.033AsnGly: 4.033 ± 0.08	1.1AsnHis: 1.1 ± 0.029	5.781AsnIle: 5.781 ± 0.084	5.528AsnLys: 5.528 ± 0.084	6.395AsnLeu: 6.395 ± 0.09	1.233AsnMet: 1.233 ± 0.033	4.985AsnAsn: 4.985 ± 0.112	2.776AsnPro: 2.776 ± 0.048	2.406AsnGln: 2.406 ± 0.051	2.003AsnArg: 2.003 ± 0.041	4.721AsnSer: 4.721 ± 0.075	4.063AsnThr: 4.063 ± 0.081	3.785AsnVal: 3.785 ± 0.06	0.918AsnTrp: 0.918 ± 0.032	3.23AsnTyr: 3.23 ± 0.058	0.0AsnXaa: 0.0 ± 0.0
Pro	1.582ProAla: 1.582 ± 0.042	0.19ProCys: 0.19 ± 0.012	1.673ProAsp: 1.673 ± 0.033	2.473ProGlu: 2.473 ± 0.052	1.911ProPhe: 1.911 ± 0.043	1.522ProGly: 1.522 ± 0.04	0.563ProHis: 0.563 ± 0.019	2.686ProIle: 2.686 ± 0.051	2.756ProLys: 2.756 ± 0.052	2.678ProLeu: 2.678 ± 0.047	0.569ProMet: 0.569 ± 0.026	2.227ProAsn: 2.227 ± 0.046	0.668ProPro: 0.668 ± 0.026	0.88ProGln: 0.88 ± 0.027	0.818ProArg: 0.818 ± 0.027	1.969ProSer: 1.969 ± 0.045	2.005ProThr: 2.005 ± 0.044	1.966ProVal: 1.966 ± 0.047	0.333ProTrp: 0.333 ± 0.019	1.215ProTyr: 1.215 ± 0.034	0.0ProXaa: 0.0 ± 0.0
Gln	1.546GlnAla: 1.546 ± 0.043	0.141GlnCys: 0.141 ± 0.011	1.469GlnAsp: 1.469 ± 0.037	2.31GlnGlu: 2.31 ± 0.051	1.686GlnPhe: 1.686 ± 0.04	1.574GlnGly: 1.574 ± 0.036	0.481GlnHis: 0.481 ± 0.021	2.734GlnIle: 2.734 ± 0.045	3.096GlnLys: 3.096 ± 0.053	3.071GlnLeu: 3.071 ± 0.051	0.702GlnMet: 0.702 ± 0.029	2.195GlnAsn: 2.195 ± 0.05	0.894GlnPro: 0.894 ± 0.029	1.316GlnGln: 1.316 ± 0.034	1.041GlnArg: 1.041 ± 0.031	1.666GlnSer: 1.666 ± 0.04	1.804GlnThr: 1.804 ± 0.062	1.694GlnVal: 1.694 ± 0.04	0.281GlnTrp: 0.281 ± 0.014	1.201GlnTyr: 1.201 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	1.76ArgAla: 1.76 ± 0.04	0.176ArgCys: 0.176 ± 0.012	1.49ArgAsp: 1.49 ± 0.041	1.892ArgGlu: 1.892 ± 0.043	1.753ArgPhe: 1.753 ± 0.037	1.73ArgGly: 1.73 ± 0.042	0.481ArgHis: 0.481 ± 0.021	2.638ArgIle: 2.638 ± 0.053	2.81ArgLys: 2.81 ± 0.054	2.71ArgLeu: 2.71 ± 0.048	0.75ArgMet: 0.75 ± 0.031	2.043ArgAsn: 2.043 ± 0.043	0.937ArgPro: 0.937 ± 0.029	0.859ArgGln: 0.859 ± 0.033	1.068ArgArg: 1.068 ± 0.037	1.639ArgSer: 1.639 ± 0.04	1.79ArgThr: 1.79 ± 0.042	1.829ArgVal: 1.829 ± 0.038	0.315ArgTrp: 0.315 ± 0.017	1.303ArgTyr: 1.303 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	3.588SerAla: 3.588 ± 0.064	0.612SerCys: 0.612 ± 0.024	3.704SerAsp: 3.704 ± 0.065	4.333SerGlu: 4.333 ± 0.061	4.202SerPhe: 4.202 ± 0.064	4.283SerGly: 4.283 ± 0.061	0.959SerHis: 0.959 ± 0.029	5.813SerIle: 5.813 ± 0.085	5.819SerLys: 5.819 ± 0.08	6.309SerLeu: 6.309 ± 0.102	1.226SerMet: 1.226 ± 0.032	4.283SerAsn: 4.283 ± 0.094	1.887SerPro: 1.887 ± 0.039	1.9SerGln: 1.9 ± 0.043	1.905SerArg: 1.905 ± 0.04	4.666SerSer: 4.666 ± 0.085	3.736SerThr: 3.736 ± 0.067	3.911SerVal: 3.911 ± 0.061	0.765SerTrp: 0.765 ± 0.026	2.895SerTyr: 2.895 ± 0.06	0.0SerXaa: 0.0 ± 0.0
Thr	3.592ThrAla: 3.592 ± 0.063	0.362ThrCys: 0.362 ± 0.017	3.487ThrAsp: 3.487 ± 0.065	3.596ThrGlu: 3.596 ± 0.065	3.306ThrPhe: 3.306 ± 0.057	3.75ThrGly: 3.75 ± 0.065	0.952ThrHis: 0.952 ± 0.026	5.566ThrIle: 5.566 ± 0.089	4.65ThrLys: 4.65 ± 0.068	5.39ThrLeu: 5.39 ± 0.076	0.894ThrMet: 0.894 ± 0.03	3.849ThrAsn: 3.849 ± 0.077	2.179ThrPro: 2.179 ± 0.054	1.736ThrGln: 1.736 ± 0.048	1.489ThrArg: 1.489 ± 0.036	4.271ThrSer: 4.271 ± 0.085	3.76ThrThr: 3.76 ± 0.085	3.647ThrVal: 3.647 ± 0.073	0.581ThrTrp: 0.581 ± 0.026	2.397ThrTyr: 2.397 ± 0.053	0.0ThrXaa: 0.0 ± 0.0
Val	3.867ValAla: 3.867 ± 0.07	0.461ValCys: 0.461 ± 0.021	3.611ValAsp: 3.611 ± 0.064	3.669ValGlu: 3.669 ± 0.068	3.45ValPhe: 3.45 ± 0.06	3.519ValGly: 3.519 ± 0.061	0.934ValHis: 0.934 ± 0.032	4.783ValIle: 4.783 ± 0.067	4.763ValLys: 4.763 ± 0.06	5.801ValLeu: 5.801 ± 0.085	1.071ValMet: 1.071 ± 0.035	3.62ValAsn: 3.62 ± 0.067	1.844ValPro: 1.844 ± 0.042	1.535ValGln: 1.535 ± 0.037	1.756ValArg: 1.756 ± 0.043	4.479ValSer: 4.479 ± 0.076	3.619ValThr: 3.619 ± 0.093	3.839ValVal: 3.839 ± 0.078	0.658ValTrp: 0.658 ± 0.024	2.209ValTyr: 2.209 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.577TrpAla: 0.577 ± 0.031	0.106TrpCys: 0.106 ± 0.01	0.565TrpAsp: 0.565 ± 0.025	0.64TrpGlu: 0.64 ± 0.023	0.596TrpPhe: 0.596 ± 0.025	0.639TrpGly: 0.639 ± 0.023	0.224TrpHis: 0.224 ± 0.014	0.728TrpIle: 0.728 ± 0.03	0.898TrpLys: 0.898 ± 0.032	0.937TrpLeu: 0.937 ± 0.031	0.306TrpMet: 0.306 ± 0.018	0.716TrpAsn: 0.716 ± 0.023	0.218TrpPro: 0.218 ± 0.013	0.436TrpGln: 0.436 ± 0.019	0.371TrpArg: 0.371 ± 0.018	0.672TrpSer: 0.672 ± 0.03	0.499TrpThr: 0.499 ± 0.024	0.612TrpVal: 0.612 ± 0.025	0.142TrpTrp: 0.142 ± 0.01	0.425TrpTyr: 0.425 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.265TyrAla: 2.265 ± 0.049	0.288TyrCys: 0.288 ± 0.017	2.114TyrAsp: 2.114 ± 0.045	2.087TyrGlu: 2.087 ± 0.044	2.522TyrPhe: 2.522 ± 0.053	2.373TyrGly: 2.373 ± 0.045	0.742TyrHis: 0.742 ± 0.028	2.908TyrIle: 2.908 ± 0.056	3.604TyrLys: 3.604 ± 0.066	3.868TyrLeu: 3.868 ± 0.059	0.68TyrMet: 0.68 ± 0.027	2.899TyrAsn: 2.899 ± 0.064	1.462TyrPro: 1.462 ± 0.037	1.614TyrGln: 1.614 ± 0.044	1.379TyrArg: 1.379 ± 0.038	2.737TyrSer: 2.737 ± 0.058	2.399TyrThr: 2.399 ± 0.048	2.034TyrVal: 2.034 ± 0.037	0.47TyrTrp: 0.47 ± 0.021	1.787TyrTyr: 1.787 ± 0.046	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3350 proteins (1171335 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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