Hirame rhabdovirus (strain Korea/CA 9703/1997) (HIRRV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Gammarhabdovirinae; Novirhabdovirus; Hirame novirhabdovirus

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q9QL89|NCAP_HIRRV Nucleoprotein OS=Hirame rhabdovirus (strain Korea/CA 9703/1997) OX=453457 GN=N PE=3 SV=1
MM1 pKa = 7.71ANLKK5 pKa = 10.57EE6 pKa = 4.19EE7 pKa = 4.3FAGLRR12 pKa = 11.84GVKK15 pKa = 10.14GGALEE20 pKa = 4.92DD21 pKa = 3.86SGTEE25 pKa = 3.88YY26 pKa = 11.28DD27 pKa = 3.56PTKK30 pKa = 10.77INLTLYY36 pKa = 9.17GTDD39 pKa = 3.24KK40 pKa = 11.1LYY42 pKa = 10.24TLAIIKK48 pKa = 10.17RR49 pKa = 11.84AVSQVGGSQTNKK61 pKa = 10.74ALGILCAFVTSEE73 pKa = 4.54NNPDD77 pKa = 3.26MTDD80 pKa = 3.05AAVKK84 pKa = 10.33LLVDD88 pKa = 3.48MRR90 pKa = 11.84FKK92 pKa = 11.25VDD94 pKa = 3.42VVPVDD99 pKa = 4.87DD100 pKa = 5.17RR101 pKa = 11.84LGDD104 pKa = 3.83NLDD107 pKa = 4.36DD108 pKa = 5.08PNSKK112 pKa = 10.06LAEE115 pKa = 4.16VLTEE119 pKa = 3.94EE120 pKa = 5.06NMVDD124 pKa = 3.75LVKK127 pKa = 11.04GLLFTCALMVKK138 pKa = 10.31YY139 pKa = 10.39DD140 pKa = 3.7VDD142 pKa = 4.63KK143 pKa = 10.85MATYY147 pKa = 9.84CQQKK151 pKa = 10.36LEE153 pKa = 4.33RR154 pKa = 11.84LANSQGLNEE163 pKa = 4.5LTLISTSRR171 pKa = 11.84AVLARR176 pKa = 11.84IGAAVRR182 pKa = 11.84PGQKK186 pKa = 8.37LTKK189 pKa = 9.73AIYY192 pKa = 9.93GIILINLMDD201 pKa = 5.24PATAARR207 pKa = 11.84AKK209 pKa = 9.97ALCAMRR215 pKa = 11.84LSGTGMTMVGLFNQASKK232 pKa = 11.41NLGAPPADD240 pKa = 3.93LLEE243 pKa = 4.49DD244 pKa = 3.68LCMKK248 pKa = 10.58SIIDD252 pKa = 3.46SARR255 pKa = 11.84RR256 pKa = 11.84IVKK259 pKa = 10.06LMRR262 pKa = 11.84IVADD266 pKa = 3.9VEE268 pKa = 4.88DD269 pKa = 4.14MTAKK273 pKa = 9.91YY274 pKa = 10.47AIMMSRR280 pKa = 11.84MLGDD284 pKa = 4.73GYY286 pKa = 10.94FKK288 pKa = 10.93SYY290 pKa = 10.79GINEE294 pKa = 4.2NSRR297 pKa = 11.84ITCILMNINEE307 pKa = 4.38QYY309 pKa = 11.33DD310 pKa = 3.43EE311 pKa = 4.33GTTGGLAGVRR321 pKa = 11.84VSPPFRR327 pKa = 11.84KK328 pKa = 9.57LATEE332 pKa = 4.32IARR335 pKa = 11.84LLVKK339 pKa = 10.38KK340 pKa = 10.82YY341 pKa = 10.44DD342 pKa = 4.05GNGSAGPGASDD353 pKa = 4.26LVRR356 pKa = 11.84QAEE359 pKa = 4.04QAAQEE364 pKa = 4.47TEE366 pKa = 4.12GEE368 pKa = 3.89DD369 pKa = 3.75DD370 pKa = 4.68KK371 pKa = 11.86YY372 pKa = 11.52DD373 pKa = 3.71EE374 pKa = 4.96EE375 pKa = 5.78GEE377 pKa = 4.04EE378 pKa = 4.66DD379 pKa = 3.51GGEE382 pKa = 4.34GEE384 pKa = 4.27GGEE387 pKa = 4.19DD388 pKa = 3.12EE389 pKa = 5.12EE390 pKa = 5.67YY391 pKa = 10.98YY392 pKa = 11.26

Molecular weight: 42.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q82080|NV_HIRRV Non-virion protein OS=Hirame rhabdovirus (strain Korea/CA 9703/1997) OX=453457 GN=NV PE=3 SV=1
MM1 pKa = 7.42SLFKK5 pKa = 9.97RR6 pKa = 11.84TKK8 pKa = 8.77KK9 pKa = 9.29TILIPPPHH17 pKa = 6.93LLSGDD22 pKa = 3.66EE23 pKa = 4.58DD24 pKa = 4.01RR25 pKa = 11.84VTVLNAEE32 pKa = 4.3GEE34 pKa = 4.33IKK36 pKa = 10.39ISGKK40 pKa = 10.33RR41 pKa = 11.84PTTLDD46 pKa = 3.1EE47 pKa = 4.65KK48 pKa = 10.5IYY50 pKa = 11.15YY51 pKa = 10.56SMSLAAAILGGNLHH65 pKa = 7.13PSFQSLTYY73 pKa = 10.69LFQQEE78 pKa = 4.49MEE80 pKa = 4.35FGSTIEE86 pKa = 4.06KK87 pKa = 10.78VNFGSRR93 pKa = 11.84KK94 pKa = 8.6PAPLTPIRR102 pKa = 11.84WQKK105 pKa = 10.16PEE107 pKa = 3.8KK108 pKa = 10.36CSFRR112 pKa = 11.84PQPLDD117 pKa = 3.51KK118 pKa = 10.59KK119 pKa = 10.35IPPQIYY125 pKa = 7.77TVSVEE130 pKa = 3.92GATITFSGRR139 pKa = 11.84FLFSASHH146 pKa = 6.24VGCDD150 pKa = 3.62DD151 pKa = 3.8NRR153 pKa = 11.84VKK155 pKa = 10.89LAGLDD160 pKa = 3.79GFITSPNYY168 pKa = 10.12QRR170 pKa = 11.84VKK172 pKa = 10.61DD173 pKa = 4.04YY174 pKa = 10.97YY175 pKa = 10.66AQEE178 pKa = 4.14TVLALSFEE186 pKa = 4.37IPTKK190 pKa = 10.29KK191 pKa = 10.58GKK193 pKa = 9.99

Molecular weight: 21.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3417

111

1986

569.5

63.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.053 ± 0.625

1.405 ± 0.275

6.263 ± 0.457

6.555 ± 0.794

3.629 ± 0.424

6.292 ± 0.746

2.195 ± 0.54

5.999 ± 0.726

6.204 ± 0.469

11.062 ± 0.952

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.488 ± 0.466

3.161 ± 0.381

4.565 ± 0.596

3.395 ± 0.348

6.263 ± 0.548

7.112 ± 0.566

6.702 ± 0.588

5.531 ± 0.632

1.258 ± 0.36

2.868 ± 0.538
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski