Lachnospiraceae bacterium A4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; unclassified Lachnospiraceae

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6440 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9JA65|R9JA65_9FIRM Uncharacterized protein (Fragment) OS=Lachnospiraceae bacterium A4 OX=397291 GN=C804_02945 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.38LKK4 pKa = 10.68RR5 pKa = 11.84LMALGMAAVMTMSLTACGGSGDD27 pKa = 4.09TPSTNSNTASNNNDD41 pKa = 2.96SNAAADD47 pKa = 3.85NDD49 pKa = 4.3AAADD53 pKa = 3.94DD54 pKa = 5.04ANAADD59 pKa = 5.74DD60 pKa = 4.81SAADD64 pKa = 4.1DD65 pKa = 4.75ANAADD70 pKa = 4.68DD71 pKa = 3.93SAAEE75 pKa = 4.39GGLTYY80 pKa = 10.98GSITLGEE87 pKa = 5.02DD88 pKa = 3.51YY89 pKa = 11.04TDD91 pKa = 3.44LTASIKK97 pKa = 10.28FIHH100 pKa = 6.62HH101 pKa = 5.46KK102 pKa = 8.62TDD104 pKa = 3.48RR105 pKa = 11.84EE106 pKa = 4.12QDD108 pKa = 3.12GSMAKK113 pKa = 10.45YY114 pKa = 10.04IEE116 pKa = 4.86AFNQEE121 pKa = 4.26YY122 pKa = 10.3PNITVEE128 pKa = 4.3TEE130 pKa = 4.01GITDD134 pKa = 3.73YY135 pKa = 11.58AQTALLRR142 pKa = 11.84LPTGEE147 pKa = 3.76AWGDD151 pKa = 3.33VMFIPSIDD159 pKa = 3.56MVQLADD165 pKa = 3.54YY166 pKa = 7.29FIPYY170 pKa = 8.26GTVDD174 pKa = 3.32EE175 pKa = 4.29MSEE178 pKa = 4.19YY179 pKa = 11.45VNFADD184 pKa = 3.26QWKK187 pKa = 10.52FEE189 pKa = 4.36GNCYY193 pKa = 9.92GVGYY197 pKa = 9.11MGNAQGILYY206 pKa = 9.0NKK208 pKa = 9.97KK209 pKa = 8.58VFADD213 pKa = 3.46AGVTEE218 pKa = 4.73LPKK221 pKa = 10.73TPTEE225 pKa = 4.86FIAALQQIKK234 pKa = 10.73DD235 pKa = 3.68NTDD238 pKa = 4.57AIPLYY243 pKa = 10.26TNYY246 pKa = 10.71AAGWTMGGQWDD257 pKa = 4.59AYY259 pKa = 10.42LGAITTGDD267 pKa = 3.96TTWLNQKK274 pKa = 9.03FAHH277 pKa = 5.95SAEE280 pKa = 4.13PFKK283 pKa = 11.55DD284 pKa = 3.43NGDD287 pKa = 3.5EE288 pKa = 4.01TGAYY292 pKa = 9.86ALYY295 pKa = 10.22KK296 pKa = 10.11ILYY299 pKa = 8.87DD300 pKa = 3.67AVANGLTEE308 pKa = 5.37DD309 pKa = 5.44DD310 pKa = 4.16YY311 pKa = 10.86TTTDD315 pKa = 2.86WEE317 pKa = 4.93GCKK320 pKa = 10.76GMINRR325 pKa = 11.84GEE327 pKa = 4.23IGTMVLGSWAVAQMKK342 pKa = 9.84EE343 pKa = 3.68ADD345 pKa = 4.06ANGGDD350 pKa = 3.7IGYY353 pKa = 8.11MPFPISVGGKK363 pKa = 8.89QYY365 pKa = 10.33ATAGPDD371 pKa = 3.12YY372 pKa = 10.71CYY374 pKa = 10.73GINKK378 pKa = 9.28EE379 pKa = 4.15SSADD383 pKa = 3.55NQAAAMVFVKK393 pKa = 10.03WMVEE397 pKa = 3.68KK398 pKa = 10.94SGWCDD403 pKa = 3.25NEE405 pKa = 3.8GGYY408 pKa = 9.62PISKK412 pKa = 8.26TAPTSFVFDD421 pKa = 3.36GVEE424 pKa = 4.12VVNNEE429 pKa = 3.86AAPDD433 pKa = 3.93DD434 pKa = 4.5EE435 pKa = 6.91ADD437 pKa = 5.75LMNQMNAEE445 pKa = 4.16CEE447 pKa = 4.3LNFNNGGDD455 pKa = 3.31RR456 pKa = 11.84KK457 pKa = 9.72VQEE460 pKa = 4.22IVEE463 pKa = 4.12AAATGSMTYY472 pKa = 10.79DD473 pKa = 3.24EE474 pKa = 6.06IMANWTALWNDD485 pKa = 3.5AQSSLGVDD493 pKa = 3.42VTEE496 pKa = 4.38

Molecular weight: 53.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9JUY2|R9JUY2_9FIRM Uncharacterized protein OS=Lachnospiraceae bacterium A4 OX=397291 GN=C804_00046 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.95KK9 pKa = 7.58RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.17VHH16 pKa = 5.89GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.8VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.92GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6440

0

6440

1943585

20

5621

301.8

34.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.351 ± 0.034

1.591 ± 0.014

5.774 ± 0.027

7.793 ± 0.037

4.099 ± 0.023

6.464 ± 0.029

1.77 ± 0.014

7.107 ± 0.032

6.796 ± 0.029

8.818 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.046 ± 0.019

4.613 ± 0.024

3.025 ± 0.017

3.673 ± 0.022

4.754 ± 0.026

5.835 ± 0.026

5.379 ± 0.035

6.462 ± 0.027

1.021 ± 0.011

4.627 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski