Amino acid dipepetide frequency for Lactococcus phage AM9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.028AlaAla: 0.028 ± 0.024
0.279AlaCys: 0.279 ± 0.085
2.619AlaAsp: 2.619 ± 0.276
3.148AlaGlu: 3.148 ± 0.309
1.755AlaPhe: 1.755 ± 0.191
3.621AlaGly: 3.621 ± 0.503
0.864AlaHis: 0.864 ± 0.141
3.816AlaIle: 3.816 ± 0.411
5.265AlaLys: 5.265 ± 0.583
4.485AlaLeu: 4.485 ± 0.527
1.476AlaMet: 1.476 ± 0.237
2.841AlaAsn: 2.841 ± 0.276
1.031AlaPro: 1.031 ± 0.225
2.535AlaGln: 2.535 ± 0.373
1.727AlaArg: 1.727 ± 0.251
3.231AlaSer: 3.231 ± 0.304
3.231AlaThr: 3.231 ± 0.402
3.315AlaVal: 3.315 ± 0.312
0.474AlaTrp: 0.474 ± 0.106
2.646AlaTyr: 2.646 ± 0.261
0.0AlaXaa: 0.0 ± 0.0
Cys
0.223CysAla: 0.223 ± 0.093
0.111CysCys: 0.111 ± 0.052
0.446CysAsp: 0.446 ± 0.13
0.585CysGlu: 0.585 ± 0.158
0.279CysPhe: 0.279 ± 0.087
0.501CysGly: 0.501 ± 0.173
0.084CysHis: 0.084 ± 0.058
0.557CysIle: 0.557 ± 0.128
0.585CysLys: 0.585 ± 0.134
0.501CysLeu: 0.501 ± 0.122
0.056CysMet: 0.056 ± 0.039
0.362CysAsn: 0.362 ± 0.101
0.306CysPro: 0.306 ± 0.131
0.306CysGln: 0.306 ± 0.095
0.418CysArg: 0.418 ± 0.109
0.752CysSer: 0.752 ± 0.158
0.139CysThr: 0.139 ± 0.063
0.362CysVal: 0.362 ± 0.105
0.111CysTrp: 0.111 ± 0.05
0.306CysTyr: 0.306 ± 0.095
0.0CysXaa: 0.0 ± 0.0
Asp
2.479AspAla: 2.479 ± 0.313
0.418AspCys: 0.418 ± 0.129
4.568AspAsp: 4.568 ± 0.416
6.156AspGlu: 6.156 ± 0.376
4.095AspPhe: 4.095 ± 0.352
4.68AspGly: 4.68 ± 0.364
0.474AspHis: 0.474 ± 0.16
6.156AspIle: 6.156 ± 0.477
7.048AspLys: 7.048 ± 0.502
5.655AspLeu: 5.655 ± 0.518
1.839AspMet: 1.839 ± 0.189
4.736AspAsn: 4.736 ± 0.349
0.947AspPro: 0.947 ± 0.183
1.003AspGln: 1.003 ± 0.187
2.117AspArg: 2.117 ± 0.273
4.401AspSer: 4.401 ± 0.46
3.705AspThr: 3.705 ± 0.348
4.457AspVal: 4.457 ± 0.349
0.724AspTrp: 0.724 ± 0.14
3.092AspTyr: 3.092 ± 0.288
0.0AspXaa: 0.0 ± 0.0
Glu
3.984GluAla: 3.984 ± 0.448
0.557GluCys: 0.557 ± 0.141
5.961GluAsp: 5.961 ± 0.529
6.908GluGlu: 6.908 ± 0.514
3.594GluPhe: 3.594 ± 0.347
3.148GluGly: 3.148 ± 0.326
1.309GluHis: 1.309 ± 0.217
6.797GluIle: 6.797 ± 0.303
7.41GluLys: 7.41 ± 0.567
7.688GluLeu: 7.688 ± 0.524
2.229GluMet: 2.229 ± 0.243
5.321GluAsn: 5.321 ± 0.343
1.086GluPro: 1.086 ± 0.197
2.869GluGln: 2.869 ± 0.217
2.34GluArg: 2.34 ± 0.266
5.404GluSer: 5.404 ± 0.443
4.262GluThr: 4.262 ± 0.351
4.986GluVal: 4.986 ± 0.427
0.78GluTrp: 0.78 ± 0.14
4.513GluTyr: 4.513 ± 0.413
0.0GluXaa: 0.0 ± 0.0
Phe
2.006PheAla: 2.006 ± 0.249
0.39PheCys: 0.39 ± 0.124
3.844PheAsp: 3.844 ± 0.366
4.151PheGlu: 4.151 ± 0.412
1.309PhePhe: 1.309 ± 0.234
2.646PheGly: 2.646 ± 0.237
0.474PheHis: 0.474 ± 0.121
3.009PheIle: 3.009 ± 0.305
4.151PheLys: 4.151 ± 0.329
3.51PheLeu: 3.51 ± 0.397
1.449PheMet: 1.449 ± 0.218
3.287PheAsn: 3.287 ± 0.303
0.919PhePro: 0.919 ± 0.158
1.393PheGln: 1.393 ± 0.187
1.588PheArg: 1.588 ± 0.212
2.897PheSer: 2.897 ± 0.312
3.064PheThr: 3.064 ± 0.266
2.73PheVal: 2.73 ± 0.294
0.446PheTrp: 0.446 ± 0.097
2.201PheTyr: 2.201 ± 0.314
0.0PheXaa: 0.0 ± 0.0
Gly
2.451GlyAla: 2.451 ± 0.322
0.306GlyCys: 0.306 ± 0.093
3.566GlyAsp: 3.566 ± 0.273
4.151GlyGlu: 4.151 ± 0.324
3.315GlyPhe: 3.315 ± 0.253
3.454GlyGly: 3.454 ± 0.455
0.919GlyHis: 0.919 ± 0.147
4.819GlyIle: 4.819 ± 0.379
4.819GlyLys: 4.819 ± 0.423
4.568GlyLeu: 4.568 ± 0.33
1.95GlyMet: 1.95 ± 0.197
4.262GlyAsn: 4.262 ± 0.43
0.0GlyPro: 0.0 ± 0.0
1.504GlyGln: 1.504 ± 0.175
2.201GlyArg: 2.201 ± 0.269
3.371GlySer: 3.371 ± 0.327
3.649GlyThr: 3.649 ± 0.397
3.705GlyVal: 3.705 ± 0.263
0.808GlyTrp: 0.808 ± 0.176
3.064GlyTyr: 3.064 ± 0.308
0.0GlyXaa: 0.0 ± 0.0
His
0.557HisAla: 0.557 ± 0.111
0.139HisCys: 0.139 ± 0.055
1.198HisAsp: 1.198 ± 0.182
1.003HisGlu: 1.003 ± 0.174
0.808HisPhe: 0.808 ± 0.178
1.059HisGly: 1.059 ± 0.242
0.362HisHis: 0.362 ± 0.127
1.281HisIle: 1.281 ± 0.22
1.365HisLys: 1.365 ± 0.216
1.003HisLeu: 1.003 ± 0.16
0.251HisMet: 0.251 ± 0.088
1.086HisAsn: 1.086 ± 0.238
0.418HisPro: 0.418 ± 0.091
0.474HisGln: 0.474 ± 0.104
0.529HisArg: 0.529 ± 0.141
1.003HisSer: 1.003 ± 0.141
0.864HisThr: 0.864 ± 0.171
0.613HisVal: 0.613 ± 0.153
0.195HisTrp: 0.195 ± 0.08
0.975HisTyr: 0.975 ± 0.195
0.0HisXaa: 0.0 ± 0.0
Ile
3.956IleAla: 3.956 ± 0.33
0.557IleCys: 0.557 ± 0.116
5.822IleAsp: 5.822 ± 0.469
6.518IleGlu: 6.518 ± 0.451
3.176IlePhe: 3.176 ± 0.331
3.538IleGly: 3.538 ± 0.347
1.17IleHis: 1.17 ± 0.177
5.404IleIle: 5.404 ± 0.543
8.246IleLys: 8.246 ± 0.437
5.878IleLeu: 5.878 ± 0.43
1.309IleMet: 1.309 ± 0.171
5.516IleAsn: 5.516 ± 0.522
2.451IlePro: 2.451 ± 0.272
2.507IleGln: 2.507 ± 0.29
2.368IleArg: 2.368 ± 0.235
5.265IleSer: 5.265 ± 0.464
4.374IleThr: 4.374 ± 0.5
5.153IleVal: 5.153 ± 0.393
0.696IleTrp: 0.696 ± 0.136
2.869IleTyr: 2.869 ± 0.307
0.0IleXaa: 0.0 ± 0.0
Lys
4.931LysAla: 4.931 ± 0.475
0.557LysCys: 0.557 ± 0.158
6.658LysAsp: 6.658 ± 0.468
10.084LysGlu: 10.084 ± 0.553
3.454LysPhe: 3.454 ± 0.341
4.847LysGly: 4.847 ± 0.362
1.783LysHis: 1.783 ± 0.257
6.602LysIle: 6.602 ± 0.448
7.8LysLys: 7.8 ± 0.557
8.106LysLeu: 8.106 ± 0.525
3.064LysMet: 3.064 ± 0.295
5.933LysAsn: 5.933 ± 0.438
1.978LysPro: 1.978 ± 0.231
3.12LysGln: 3.12 ± 0.27
3.371LysArg: 3.371 ± 0.326
4.819LysSer: 4.819 ± 0.479
5.655LysThr: 5.655 ± 0.475
5.153LysVal: 5.153 ± 0.334
1.003LysTrp: 1.003 ± 0.164
3.928LysTyr: 3.928 ± 0.326
0.0LysXaa: 0.0 ± 0.0
Leu
4.708LeuAla: 4.708 ± 0.374
0.474LeuCys: 0.474 ± 0.113
6.268LeuAsp: 6.268 ± 0.388
6.128LeuGlu: 6.128 ± 0.422
3.789LeuPhe: 3.789 ± 0.415
4.736LeuGly: 4.736 ± 0.461
1.142LeuHis: 1.142 ± 0.219
5.738LeuIle: 5.738 ± 0.444
7.466LeuLys: 7.466 ± 0.501
6.24LeuLeu: 6.24 ± 0.427
2.034LeuMet: 2.034 ± 0.239
5.07LeuAsn: 5.07 ± 0.378
2.145LeuPro: 2.145 ± 0.209
2.674LeuGln: 2.674 ± 0.359
2.758LeuArg: 2.758 ± 0.292
6.435LeuSer: 6.435 ± 0.428
5.153LeuThr: 5.153 ± 0.344
4.568LeuVal: 4.568 ± 0.381
0.585LeuTrp: 0.585 ± 0.143
3.176LeuTyr: 3.176 ± 0.379
0.0LeuXaa: 0.0 ± 0.0
Met
1.839MetAla: 1.839 ± 0.208
0.084MetCys: 0.084 ± 0.049
1.56MetAsp: 1.56 ± 0.234
1.978MetGlu: 1.978 ± 0.247
1.226MetPhe: 1.226 ± 0.193
1.365MetGly: 1.365 ± 0.205
0.223MetHis: 0.223 ± 0.07
1.783MetIle: 1.783 ± 0.214
2.591MetLys: 2.591 ± 0.271
1.866MetLeu: 1.866 ± 0.237
0.78MetMet: 0.78 ± 0.144
1.978MetAsn: 1.978 ± 0.239
0.641MetPro: 0.641 ± 0.126
1.226MetGln: 1.226 ± 0.249
0.724MetArg: 0.724 ± 0.153
1.95MetSer: 1.95 ± 0.224
2.034MetThr: 2.034 ± 0.231
1.56MetVal: 1.56 ± 0.22
0.279MetTrp: 0.279 ± 0.083
1.003MetTyr: 1.003 ± 0.18
0.0MetXaa: 0.0 ± 0.0
Asn
3.176AsnAla: 3.176 ± 0.306
0.39AsnCys: 0.39 ± 0.125
3.315AsnAsp: 3.315 ± 0.258
4.958AsnGlu: 4.958 ± 0.458
3.092AsnPhe: 3.092 ± 0.314
4.346AsnGly: 4.346 ± 0.351
0.975AsnHis: 0.975 ± 0.191
5.683AsnIle: 5.683 ± 0.39
6.073AsnLys: 6.073 ± 0.413
5.599AsnLeu: 5.599 ± 0.433
1.56AsnMet: 1.56 ± 0.201
4.039AsnAsn: 4.039 ± 0.398
2.201AsnPro: 2.201 ± 0.313
2.841AsnGln: 2.841 ± 0.335
2.117AsnArg: 2.117 ± 0.188
4.262AsnSer: 4.262 ± 0.362
3.287AsnThr: 3.287 ± 0.25
3.872AsnVal: 3.872 ± 0.262
0.613AsnTrp: 0.613 ± 0.131
2.646AsnTyr: 2.646 ± 0.289
0.0AsnXaa: 0.0 ± 0.0
Pro
1.17ProAla: 1.17 ± 0.21
0.167ProCys: 0.167 ± 0.076
1.727ProAsp: 1.727 ± 0.242
1.56ProGlu: 1.56 ± 0.217
1.142ProPhe: 1.142 ± 0.186
0.084ProGly: 0.084 ± 0.043
0.418ProHis: 0.418 ± 0.105
1.616ProIle: 1.616 ± 0.179
2.061ProLys: 2.061 ± 0.269
1.866ProLeu: 1.866 ± 0.195
0.78ProMet: 0.78 ± 0.166
1.727ProAsn: 1.727 ± 0.211
0.334ProPro: 0.334 ± 0.093
0.724ProGln: 0.724 ± 0.145
0.641ProArg: 0.641 ± 0.142
1.671ProSer: 1.671 ± 0.231
1.393ProThr: 1.393 ± 0.221
1.866ProVal: 1.866 ± 0.193
0.084ProTrp: 0.084 ± 0.062
1.17ProTyr: 1.17 ± 0.198
0.0ProXaa: 0.0 ± 0.0
Gln
2.201GlnAla: 2.201 ± 0.372
0.223GlnCys: 0.223 ± 0.081
1.866GlnAsp: 1.866 ± 0.227
2.507GlnGlu: 2.507 ± 0.25
1.281GlnPhe: 1.281 ± 0.159
2.034GlnGly: 2.034 ± 0.239
0.724GlnHis: 0.724 ± 0.168
2.312GlnIle: 2.312 ± 0.267
3.148GlnLys: 3.148 ± 0.317
3.204GlnLeu: 3.204 ± 0.379
1.198GlnMet: 1.198 ± 0.208
2.229GlnAsn: 2.229 ± 0.228
0.752GlnPro: 0.752 ± 0.137
1.449GlnGln: 1.449 ± 0.342
1.588GlnArg: 1.588 ± 0.198
2.006GlnSer: 2.006 ± 0.305
2.089GlnThr: 2.089 ± 0.315
1.421GlnVal: 1.421 ± 0.179
0.39GlnTrp: 0.39 ± 0.116
1.449GlnTyr: 1.449 ± 0.213
0.0GlnXaa: 0.0 ± 0.0
Arg
1.309ArgAla: 1.309 ± 0.209
0.474ArgCys: 0.474 ± 0.217
2.424ArgAsp: 2.424 ± 0.259
2.841ArgGlu: 2.841 ± 0.335
1.588ArgPhe: 1.588 ± 0.226
2.201ArgGly: 2.201 ± 0.271
0.613ArgHis: 0.613 ± 0.129
2.674ArgIle: 2.674 ± 0.315
2.981ArgLys: 2.981 ± 0.346
2.451ArgLeu: 2.451 ± 0.269
0.836ArgMet: 0.836 ± 0.149
2.424ArgAsn: 2.424 ± 0.231
0.724ArgPro: 0.724 ± 0.147
1.226ArgGln: 1.226 ± 0.189
1.254ArgArg: 1.254 ± 0.197
1.839ArgSer: 1.839 ± 0.233
2.006ArgThr: 2.006 ± 0.21
2.424ArgVal: 2.424 ± 0.231
0.585ArgTrp: 0.585 ± 0.122
2.006ArgTyr: 2.006 ± 0.222
0.0ArgXaa: 0.0 ± 0.0
Ser
3.677SerAla: 3.677 ± 0.384
0.39SerCys: 0.39 ± 0.111
4.234SerAsp: 4.234 ± 0.418
5.07SerGlu: 5.07 ± 0.398
3.649SerPhe: 3.649 ± 0.366
4.234SerGly: 4.234 ± 0.43
0.78SerHis: 0.78 ± 0.159
5.126SerIle: 5.126 ± 0.425
5.906SerLys: 5.906 ± 0.402
4.903SerLeu: 4.903 ± 0.498
1.476SerMet: 1.476 ± 0.185
3.705SerAsn: 3.705 ± 0.409
1.504SerPro: 1.504 ± 0.19
2.089SerGln: 2.089 ± 0.219
2.925SerArg: 2.925 ± 0.305
4.624SerSer: 4.624 ± 0.709
3.538SerThr: 3.538 ± 0.378
3.956SerVal: 3.956 ± 0.364
0.696SerTrp: 0.696 ± 0.145
2.674SerTyr: 2.674 ± 0.346
0.0SerXaa: 0.0 ± 0.0
Thr
3.51ThrAla: 3.51 ± 0.613
0.501ThrCys: 0.501 ± 0.162
3.984ThrAsp: 3.984 ± 0.353
3.984ThrGlu: 3.984 ± 0.348
2.925ThrPhe: 2.925 ± 0.281
3.287ThrGly: 3.287 ± 0.316
1.059ThrHis: 1.059 ± 0.172
4.541ThrIle: 4.541 ± 0.488
5.599ThrLys: 5.599 ± 0.409
5.014ThrLeu: 5.014 ± 0.377
1.449ThrMet: 1.449 ± 0.208
3.399ThrAsn: 3.399 ± 0.289
1.866ThrPro: 1.866 ± 0.278
1.588ThrGln: 1.588 ± 0.196
2.117ThrArg: 2.117 ± 0.218
3.956ThrSer: 3.956 ± 0.409
3.566ThrThr: 3.566 ± 0.418
4.791ThrVal: 4.791 ± 0.392
0.641ThrTrp: 0.641 ± 0.172
2.424ThrTyr: 2.424 ± 0.254
0.0ThrXaa: 0.0 ± 0.0
Val
3.343ValAla: 3.343 ± 0.342
0.501ValCys: 0.501 ± 0.117
4.763ValAsp: 4.763 ± 0.374
4.986ValGlu: 4.986 ± 0.402
2.814ValPhe: 2.814 ± 0.253
3.566ValGly: 3.566 ± 0.32
0.947ValHis: 0.947 ± 0.168
4.485ValIle: 4.485 ± 0.408
5.878ValLys: 5.878 ± 0.407
4.234ValLeu: 4.234 ± 0.389
1.56ValMet: 1.56 ± 0.225
3.343ValAsn: 3.343 ± 0.331
1.476ValPro: 1.476 ± 0.246
2.256ValGln: 2.256 ± 0.239
1.922ValArg: 1.922 ± 0.23
4.095ValSer: 4.095 ± 0.378
4.513ValThr: 4.513 ± 0.39
4.346ValVal: 4.346 ± 0.338
0.808ValTrp: 0.808 ± 0.165
2.814ValTyr: 2.814 ± 0.303
0.0ValXaa: 0.0 ± 0.0
Trp
0.39TrpAla: 0.39 ± 0.112
0.084TrpCys: 0.084 ± 0.044
0.696TrpAsp: 0.696 ± 0.152
0.836TrpGlu: 0.836 ± 0.166
0.446TrpPhe: 0.446 ± 0.122
0.641TrpGly: 0.641 ± 0.155
0.195TrpHis: 0.195 ± 0.067
0.641TrpIle: 0.641 ± 0.164
0.752TrpLys: 0.752 ± 0.154
0.947TrpLeu: 0.947 ± 0.169
0.334TrpMet: 0.334 ± 0.089
1.086TrpAsn: 1.086 ± 0.163
0.0TrpPro: 0.0 ± 0.0
0.306TrpGln: 0.306 ± 0.089
0.418TrpArg: 0.418 ± 0.107
0.501TrpSer: 0.501 ± 0.129
0.724TrpThr: 0.724 ± 0.152
0.808TrpVal: 0.808 ± 0.163
0.084TrpTrp: 0.084 ± 0.045
0.362TrpTyr: 0.362 ± 0.111
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.451TyrAla: 2.451 ± 0.215
0.39TyrCys: 0.39 ± 0.108
3.231TyrAsp: 3.231 ± 0.358
3.594TyrGlu: 3.594 ± 0.325
1.727TyrPhe: 1.727 ± 0.266
2.897TyrGly: 2.897 ± 0.25
0.669TyrHis: 0.669 ± 0.141
3.733TyrIle: 3.733 ± 0.337
3.705TyrLys: 3.705 ± 0.327
3.566TyrLeu: 3.566 ± 0.305
1.031TyrMet: 1.031 ± 0.193
2.702TyrAsn: 2.702 ± 0.294
1.421TyrPro: 1.421 ± 0.23
1.978TyrGln: 1.978 ± 0.227
1.755TyrArg: 1.755 ± 0.229
2.674TyrSer: 2.674 ± 0.282
2.953TyrThr: 2.953 ± 0.349
2.563TyrVal: 2.563 ± 0.271
0.279TyrTrp: 0.279 ± 0.096
1.839TyrTyr: 1.839 ± 0.288
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 179 proteins (35899 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski