Bark beetle-associated genomovirus 5

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus denpo1

Average proteome isoelectric point is 7.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A344A3Q5|A0A344A3Q5_9VIRU Replication-associated protein OS=Bark beetle-associated genomovirus 5 OX=2230900 PE=3 SV=1
MM1 pKa = 7.4LFVNSRR7 pKa = 11.84YY8 pKa = 10.23VLLTYY13 pKa = 10.38SQSNGLDD20 pKa = 2.94EE21 pKa = 4.5WAVSDD26 pKa = 4.22HH27 pKa = 6.04MSGLGAEE34 pKa = 4.59CIVARR39 pKa = 11.84EE40 pKa = 3.91NHH42 pKa = 6.36IDD44 pKa = 3.89GGTHH48 pKa = 5.55LHH50 pKa = 6.67AFVDD54 pKa = 4.91FNRR57 pKa = 11.84KK58 pKa = 8.42FRR60 pKa = 11.84SRR62 pKa = 11.84NARR65 pKa = 11.84VFDD68 pKa = 3.41VGGFHH73 pKa = 7.32PNVSPSRR80 pKa = 11.84GTPEE84 pKa = 3.81KK85 pKa = 10.76GYY87 pKa = 10.9DD88 pKa = 3.44YY89 pKa = 10.16ATKK92 pKa = 10.73DD93 pKa = 3.25GDD95 pKa = 3.98VVAGGLEE102 pKa = 4.02RR103 pKa = 11.84PAPRR107 pKa = 11.84GGLHH111 pKa = 6.16TGAHH115 pKa = 5.77RR116 pKa = 11.84VRR118 pKa = 11.84NVAHH122 pKa = 6.66LCEE125 pKa = 4.54SVDD128 pKa = 4.02EE129 pKa = 4.46FLEE132 pKa = 5.14LYY134 pKa = 10.95DD135 pKa = 3.62EE136 pKa = 4.52MEE138 pKa = 4.76RR139 pKa = 11.84GDD141 pKa = 4.1LIGRR145 pKa = 11.84FSNIRR150 pKa = 11.84AYY152 pKa = 10.57ADD154 pKa = 2.67WRR156 pKa = 11.84FRR158 pKa = 11.84VEE160 pKa = 3.89PPAYY164 pKa = 9.91SSPPGVDD171 pKa = 3.95FGSGAMDD178 pKa = 5.32GRR180 pKa = 11.84DD181 pKa = 3.29DD182 pKa = 4.53WISQSGICSGEE193 pKa = 3.81PLIGPRR199 pKa = 11.84RR200 pKa = 11.84KK201 pKa = 10.12SLVLFGPSLTGKK213 pKa = 5.84TTWARR218 pKa = 11.84SLGNHH223 pKa = 6.96IYY225 pKa = 8.65SQRR228 pKa = 11.84MLNVRR233 pKa = 11.84EE234 pKa = 3.86VAYY237 pKa = 10.87SIDD240 pKa = 3.29SARR243 pKa = 11.84YY244 pKa = 8.42HH245 pKa = 6.61VLDD248 pKa = 5.09DD249 pKa = 3.23VDD251 pKa = 4.78LRR253 pKa = 11.84YY254 pKa = 9.85FPGWKK259 pKa = 9.58DD260 pKa = 3.09WLGGMMWIPVRR271 pKa = 11.84LMYY274 pKa = 10.29RR275 pKa = 11.84DD276 pKa = 3.7VMNMQWGRR284 pKa = 11.84PCIWCNNTDD293 pKa = 3.35PRR295 pKa = 11.84DD296 pKa = 3.77VIKK299 pKa = 10.79RR300 pKa = 11.84SMAKK304 pKa = 9.87HH305 pKa = 6.7DD306 pKa = 3.9GQGDD310 pKa = 3.98GTFSQEE316 pKa = 4.08DD317 pKa = 4.44LDD319 pKa = 4.02WLEE322 pKa = 4.46ANCIFIRR329 pKa = 11.84VNDD332 pKa = 4.81PIVTFRR338 pKa = 11.84ASTEE342 pKa = 3.77

Molecular weight: 38.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A344A3Q5|A0A344A3Q5_9VIRU Replication-associated protein OS=Bark beetle-associated genomovirus 5 OX=2230900 PE=3 SV=1
MM1 pKa = 7.57SYY3 pKa = 10.55SSKK6 pKa = 10.38SRR8 pKa = 11.84SVRR11 pKa = 11.84VSRR14 pKa = 11.84RR15 pKa = 11.84FKK17 pKa = 10.23SRR19 pKa = 11.84NRR21 pKa = 11.84HH22 pKa = 5.01RR23 pKa = 11.84GSRR26 pKa = 11.84RR27 pKa = 11.84SKK29 pKa = 8.11RR30 pKa = 11.84TNRR33 pKa = 11.84RR34 pKa = 11.84SARR37 pKa = 11.84STYY40 pKa = 10.07RR41 pKa = 11.84KK42 pKa = 10.22GMTRR46 pKa = 11.84KK47 pKa = 9.9RR48 pKa = 11.84ILNITSRR55 pKa = 11.84KK56 pKa = 8.35KK57 pKa = 10.37RR58 pKa = 11.84NGMLCVTNSGTTGVGTSTFTNGALYY83 pKa = 11.2VNGSSGGWVLWAATAQDD100 pKa = 4.62LNDD103 pKa = 3.93GSSTLGTVAEE113 pKa = 4.42MAQRR117 pKa = 11.84TASTCYY123 pKa = 8.93MRR125 pKa = 11.84GLSEE129 pKa = 4.66HH130 pKa = 6.85IKK132 pKa = 10.07IQTSSAIPWFWRR144 pKa = 11.84RR145 pKa = 11.84IVFRR149 pKa = 11.84LKK151 pKa = 10.91GNPTFQAYY159 pKa = 8.12TSAPPNLTGPYY170 pKa = 8.56YY171 pKa = 10.8DD172 pKa = 3.37STAGLEE178 pKa = 3.93RR179 pKa = 11.84LLLNSNSQTVSQSGQVSAQQSVLFKK204 pKa = 10.92GRR206 pKa = 11.84AGFDD210 pKa = 3.16WNDD213 pKa = 3.53PLIAPVDD220 pKa = 3.73TARR223 pKa = 11.84VSLMYY228 pKa = 10.72DD229 pKa = 2.88KK230 pKa = 11.04TRR232 pKa = 11.84TLRR235 pKa = 11.84SGNQSGALLEE245 pKa = 4.58SKK247 pKa = 10.37LFHH250 pKa = 6.72SFNKK254 pKa = 9.98NLVYY258 pKa = 10.88DD259 pKa = 4.57DD260 pKa = 5.2DD261 pKa = 4.72EE262 pKa = 6.51SGATEE267 pKa = 3.85EE268 pKa = 3.89TSYY271 pKa = 11.4FSVQSKK277 pKa = 11.0AGMGDD282 pKa = 3.78VYY284 pKa = 11.56VLDD287 pKa = 3.55QMYY290 pKa = 10.74AGAGASATDD299 pKa = 4.13LLQFSTSSTLYY310 pKa = 7.41WHH312 pKa = 6.65EE313 pKa = 4.0RR314 pKa = 3.41

Molecular weight: 34.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

656

314

342

328.0

36.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.555 ± 0.504

1.22 ± 0.381

6.402 ± 1.481

3.659 ± 0.727

3.963 ± 0.301

9.146 ± 0.567

2.287 ± 0.663

3.354 ± 0.736

3.354 ± 0.723

7.47 ± 0.322

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.896 ± 0.228

4.726 ± 0.242

3.659 ± 0.936

2.896 ± 0.814

9.146 ± 0.058

10.366 ± 2.179

6.25 ± 1.955

6.402 ± 0.647

2.439 ± 0.346

3.811 ± 0.215
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski