Microbacterium phage Stromboli

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dismasvirus; unclassified Dismasvirus

Average proteome isoelectric point is 6.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 67 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8R0K5|A0A6G8R0K5_9CAUD Uncharacterized protein OS=Microbacterium phage Stromboli OX=2713263 GN=47 PE=4 SV=1
MM1 pKa = 7.05STFTEE6 pKa = 4.36KK7 pKa = 10.73LDD9 pKa = 3.87AALPLPTAGSLPSTRR24 pKa = 11.84TFSMVEE30 pKa = 3.93SALASSDD37 pKa = 2.77IDD39 pKa = 3.75EE40 pKa = 4.76VKK42 pKa = 11.13GEE44 pKa = 4.13LQHH47 pKa = 7.16SRR49 pKa = 11.84AHH51 pKa = 5.15YY52 pKa = 10.01QSLEE56 pKa = 3.77YY57 pKa = 10.14MLNNEE62 pKa = 3.99VAIVKK67 pKa = 8.25TIVEE71 pKa = 4.56SFPLGDD77 pKa = 5.65DD78 pKa = 3.32IAPTPEE84 pKa = 4.02TVEE87 pKa = 4.03EE88 pKa = 4.04AVQLTVRR95 pKa = 11.84VFATEE100 pKa = 4.0VLMAAYY106 pKa = 10.1DD107 pKa = 3.69SAHH110 pKa = 6.59QIGLPMAIMGLSVQGALQMLYY131 pKa = 11.08GDD133 pKa = 5.38LDD135 pKa = 3.87FSDD138 pKa = 4.37PEE140 pKa = 4.35RR141 pKa = 11.84GVVSNDD147 pKa = 3.03QIAAFIAGDD156 pKa = 4.2DD157 pKa = 3.75EE158 pKa = 4.82DD159 pKa = 3.96

Molecular weight: 17.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8R0K4|A0A6G8R0K4_9CAUD Uncharacterized protein OS=Microbacterium phage Stromboli OX=2713263 GN=55 PE=4 SV=1
MM1 pKa = 7.44TGDD4 pKa = 3.84SVISLAARR12 pKa = 11.84IVQARR17 pKa = 11.84AVANEE22 pKa = 3.8TAALLGPDD30 pKa = 2.92AWAARR35 pKa = 11.84MARR38 pKa = 11.84QEE40 pKa = 3.99LDD42 pKa = 3.37SLGRR46 pKa = 11.84QAKK49 pKa = 9.82VLFRR53 pKa = 5.08

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

67

0

67

12936

42

876

193.1

21.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.789 ± 0.304

0.433 ± 0.087

6.246 ± 0.304

6.393 ± 0.28

3.231 ± 0.148

8.588 ± 0.339

1.716 ± 0.162

3.935 ± 0.176

4.275 ± 0.277

8.612 ± 0.315

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.147

2.922 ± 0.214

5.21 ± 0.236

3.44 ± 0.188

6.347 ± 0.405

5.489 ± 0.296

6.323 ± 0.33

8.318 ± 0.317

2.033 ± 0.169

2.505 ± 0.164
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski