Amino acid dipepetide frequency for Mycobacterium phage EagleEye

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.399AlaAla: 11.399 ± 1.254
0.792AlaCys: 0.792 ± 0.199
5.73AlaAsp: 5.73 ± 0.603
6.705AlaGlu: 6.705 ± 0.752
3.231AlaPhe: 3.231 ± 0.462
7.802AlaGly: 7.802 ± 0.676
1.463AlaHis: 1.463 ± 0.312
4.998AlaIle: 4.998 ± 0.474
5.852AlaLys: 5.852 ± 0.709
8.412AlaLeu: 8.412 ± 0.82
2.804AlaMet: 2.804 ± 0.43
2.743AlaAsn: 2.743 ± 0.445
5.12AlaPro: 5.12 ± 0.748
3.048AlaGln: 3.048 ± 0.435
5.852AlaArg: 5.852 ± 0.624
4.511AlaSer: 4.511 ± 0.493
5.12AlaThr: 5.12 ± 0.465
6.096AlaVal: 6.096 ± 0.689
2.012AlaTrp: 2.012 ± 0.294
2.438AlaTyr: 2.438 ± 0.501
0.0AlaXaa: 0.0 ± 0.0
Cys
0.61CysAla: 0.61 ± 0.16
0.122CysCys: 0.122 ± 0.119
0.731CysAsp: 0.731 ± 0.202
0.731CysGlu: 0.731 ± 0.202
0.183CysPhe: 0.183 ± 0.09
0.731CysGly: 0.731 ± 0.182
0.244CysHis: 0.244 ± 0.098
0.244CysIle: 0.244 ± 0.115
0.183CysLys: 0.183 ± 0.118
0.61CysLeu: 0.61 ± 0.164
0.183CysMet: 0.183 ± 0.097
0.488CysAsn: 0.488 ± 0.155
0.549CysPro: 0.549 ± 0.19
0.305CysGln: 0.305 ± 0.132
0.61CysArg: 0.61 ± 0.21
0.792CysSer: 0.792 ± 0.229
0.488CysThr: 0.488 ± 0.153
0.731CysVal: 0.731 ± 0.199
0.366CysTrp: 0.366 ± 0.142
0.366CysTyr: 0.366 ± 0.163
0.0CysXaa: 0.0 ± 0.0
Asp
5.852AspAla: 5.852 ± 0.649
0.731AspCys: 0.731 ± 0.212
3.536AspAsp: 3.536 ± 0.468
4.084AspGlu: 4.084 ± 0.469
2.255AspPhe: 2.255 ± 0.377
5.73AspGly: 5.73 ± 0.573
1.341AspHis: 1.341 ± 0.257
3.231AspIle: 3.231 ± 0.338
3.048AspLys: 3.048 ± 0.498
6.218AspLeu: 6.218 ± 0.663
1.646AspMet: 1.646 ± 0.272
1.707AspAsn: 1.707 ± 0.317
5.181AspPro: 5.181 ± 0.532
1.585AspGln: 1.585 ± 0.283
2.987AspArg: 2.987 ± 0.432
3.414AspSer: 3.414 ± 0.451
4.206AspThr: 4.206 ± 0.494
5.303AspVal: 5.303 ± 0.539
1.585AspTrp: 1.585 ± 0.346
2.438AspTyr: 2.438 ± 0.342
0.0AspXaa: 0.0 ± 0.0
Glu
7.985GluAla: 7.985 ± 0.835
0.122GluCys: 0.122 ± 0.082
5.059GluAsp: 5.059 ± 0.602
4.45GluGlu: 4.45 ± 0.66
2.012GluPhe: 2.012 ± 0.338
5.181GluGly: 5.181 ± 0.519
1.951GluHis: 1.951 ± 0.416
4.084GluIle: 4.084 ± 0.494
2.926GluLys: 2.926 ± 0.478
6.279GluLeu: 6.279 ± 0.738
1.829GluMet: 1.829 ± 0.326
2.316GluAsn: 2.316 ± 0.284
2.804GluPro: 2.804 ± 0.421
1.951GluGln: 1.951 ± 0.339
4.572GluArg: 4.572 ± 0.561
2.987GluSer: 2.987 ± 0.482
3.596GluThr: 3.596 ± 0.467
4.633GluVal: 4.633 ± 0.558
1.463GluTrp: 1.463 ± 0.289
2.012GluTyr: 2.012 ± 0.362
0.0GluXaa: 0.0 ± 0.0
Phe
2.865PheAla: 2.865 ± 0.349
0.427PheCys: 0.427 ± 0.144
2.377PheAsp: 2.377 ± 0.432
1.951PheGlu: 1.951 ± 0.337
0.731PhePhe: 0.731 ± 0.289
3.17PheGly: 3.17 ± 0.418
0.671PheHis: 0.671 ± 0.199
1.219PheIle: 1.219 ± 0.24
1.402PheLys: 1.402 ± 0.255
2.073PheLeu: 2.073 ± 0.357
0.853PheMet: 0.853 ± 0.211
1.463PheAsn: 1.463 ± 0.259
2.255PhePro: 2.255 ± 0.387
1.341PheGln: 1.341 ± 0.269
1.951PheArg: 1.951 ± 0.318
2.377PheSer: 2.377 ± 0.372
2.865PheThr: 2.865 ± 0.364
2.012PheVal: 2.012 ± 0.3
0.61PheTrp: 0.61 ± 0.192
0.549PheTyr: 0.549 ± 0.17
0.0PheXaa: 0.0 ± 0.0
Gly
6.766GlyAla: 6.766 ± 0.992
0.792GlyCys: 0.792 ± 0.218
5.364GlyAsp: 5.364 ± 0.575
4.755GlyGlu: 4.755 ± 0.574
3.292GlyPhe: 3.292 ± 0.415
9.631GlyGly: 9.631 ± 2.167
1.829GlyHis: 1.829 ± 0.35
3.657GlyIle: 3.657 ± 0.507
4.206GlyLys: 4.206 ± 0.432
6.949GlyLeu: 6.949 ± 0.806
1.585GlyMet: 1.585 ± 0.265
2.804GlyAsn: 2.804 ± 0.444
3.596GlyPro: 3.596 ± 0.467
2.743GlyGln: 2.743 ± 0.494
4.145GlyArg: 4.145 ± 0.407
5.059GlySer: 5.059 ± 0.595
4.45GlyThr: 4.45 ± 0.593
6.218GlyVal: 6.218 ± 0.639
1.707GlyTrp: 1.707 ± 0.328
2.499GlyTyr: 2.499 ± 0.4
0.0GlyXaa: 0.0 ± 0.0
His
1.951HisAla: 1.951 ± 0.32
0.183HisCys: 0.183 ± 0.105
1.28HisAsp: 1.28 ± 0.282
1.89HisGlu: 1.89 ± 0.324
0.488HisPhe: 0.488 ± 0.186
1.524HisGly: 1.524 ± 0.354
0.488HisHis: 0.488 ± 0.156
1.158HisIle: 1.158 ± 0.236
1.036HisLys: 1.036 ± 0.255
1.158HisLeu: 1.158 ± 0.263
0.061HisMet: 0.061 ± 0.051
0.61HisAsn: 0.61 ± 0.194
1.585HisPro: 1.585 ± 0.327
0.731HisGln: 0.731 ± 0.203
1.707HisArg: 1.707 ± 0.318
0.975HisSer: 0.975 ± 0.222
1.219HisThr: 1.219 ± 0.236
0.853HisVal: 0.853 ± 0.228
0.244HisTrp: 0.244 ± 0.125
0.488HisTyr: 0.488 ± 0.267
0.0HisXaa: 0.0 ± 0.0
Ile
5.669IleAla: 5.669 ± 0.563
0.366IleCys: 0.366 ± 0.15
4.755IleAsp: 4.755 ± 0.566
4.45IleGlu: 4.45 ± 0.414
1.402IlePhe: 1.402 ± 0.273
3.657IleGly: 3.657 ± 0.509
1.097IleHis: 1.097 ± 0.251
1.951IleIle: 1.951 ± 0.316
2.926IleLys: 2.926 ± 0.362
3.353IleLeu: 3.353 ± 0.364
0.61IleMet: 0.61 ± 0.193
1.951IleAsn: 1.951 ± 0.335
3.292IlePro: 3.292 ± 0.359
1.036IleGln: 1.036 ± 0.213
3.596IleArg: 3.596 ± 0.452
2.926IleSer: 2.926 ± 0.343
2.926IleThr: 2.926 ± 0.377
2.621IleVal: 2.621 ± 0.474
0.61IleTrp: 0.61 ± 0.18
0.975IleTyr: 0.975 ± 0.239
0.0IleXaa: 0.0 ± 0.0
Lys
4.938LysAla: 4.938 ± 0.548
0.244LysCys: 0.244 ± 0.103
3.109LysAsp: 3.109 ± 0.472
2.682LysGlu: 2.682 ± 0.432
1.219LysPhe: 1.219 ± 0.261
3.292LysGly: 3.292 ± 0.434
0.61LysHis: 0.61 ± 0.178
2.743LysIle: 2.743 ± 0.409
3.657LysLys: 3.657 ± 0.493
3.718LysLeu: 3.718 ± 0.384
1.28LysMet: 1.28 ± 0.321
1.219LysAsn: 1.219 ± 0.283
2.987LysPro: 2.987 ± 0.475
1.829LysGln: 1.829 ± 0.409
3.292LysArg: 3.292 ± 0.484
2.682LysSer: 2.682 ± 0.448
3.292LysThr: 3.292 ± 0.414
4.694LysVal: 4.694 ± 0.476
0.853LysTrp: 0.853 ± 0.358
1.28LysTyr: 1.28 ± 0.279
0.0LysXaa: 0.0 ± 0.0
Leu
7.863LeuAla: 7.863 ± 0.6
0.671LeuCys: 0.671 ± 0.185
4.572LeuAsp: 4.572 ± 0.569
5.852LeuGlu: 5.852 ± 0.766
2.682LeuPhe: 2.682 ± 0.295
5.486LeuGly: 5.486 ± 0.668
1.829LeuHis: 1.829 ± 0.388
4.084LeuIle: 4.084 ± 0.461
3.84LeuLys: 3.84 ± 0.465
4.694LeuLeu: 4.694 ± 0.589
2.194LeuMet: 2.194 ± 0.364
2.377LeuAsn: 2.377 ± 0.364
4.694LeuPro: 4.694 ± 0.467
2.621LeuGln: 2.621 ± 0.52
5.852LeuArg: 5.852 ± 0.584
5.669LeuSer: 5.669 ± 0.584
4.267LeuThr: 4.267 ± 0.519
5.425LeuVal: 5.425 ± 0.572
1.89LeuTrp: 1.89 ± 0.3
2.316LeuTyr: 2.316 ± 0.417
0.0LeuXaa: 0.0 ± 0.0
Met
2.194MetAla: 2.194 ± 0.404
0.122MetCys: 0.122 ± 0.079
1.158MetAsp: 1.158 ± 0.274
1.219MetGlu: 1.219 ± 0.217
0.549MetPhe: 0.549 ± 0.158
1.158MetGly: 1.158 ± 0.328
0.488MetHis: 0.488 ± 0.15
1.341MetIle: 1.341 ± 0.383
1.28MetLys: 1.28 ± 0.254
2.012MetLeu: 2.012 ± 0.32
0.671MetMet: 0.671 ± 0.185
0.792MetAsn: 0.792 ± 0.258
1.28MetPro: 1.28 ± 0.293
0.731MetGln: 0.731 ± 0.216
1.524MetArg: 1.524 ± 0.281
2.194MetSer: 2.194 ± 0.345
2.987MetThr: 2.987 ± 0.424
1.463MetVal: 1.463 ± 0.268
0.305MetTrp: 0.305 ± 0.147
0.549MetTyr: 0.549 ± 0.149
0.0MetXaa: 0.0 ± 0.0
Asn
2.987AsnAla: 2.987 ± 0.482
0.366AsnCys: 0.366 ± 0.131
2.012AsnAsp: 2.012 ± 0.312
2.56AsnGlu: 2.56 ± 0.389
1.28AsnPhe: 1.28 ± 0.354
3.353AsnGly: 3.353 ± 0.383
0.671AsnHis: 0.671 ± 0.197
1.463AsnIle: 1.463 ± 0.255
1.463AsnLys: 1.463 ± 0.288
2.499AsnLeu: 2.499 ± 0.372
0.792AsnMet: 0.792 ± 0.188
0.671AsnAsn: 0.671 ± 0.202
1.829AsnPro: 1.829 ± 0.317
1.036AsnGln: 1.036 ± 0.217
2.133AsnArg: 2.133 ± 0.262
2.255AsnSer: 2.255 ± 0.346
1.768AsnThr: 1.768 ± 0.3
2.012AsnVal: 2.012 ± 0.337
0.731AsnTrp: 0.731 ± 0.196
0.671AsnTyr: 0.671 ± 0.192
0.0AsnXaa: 0.0 ± 0.0
Pro
4.877ProAla: 4.877 ± 0.727
0.488ProCys: 0.488 ± 0.158
4.084ProAsp: 4.084 ± 0.418
4.755ProGlu: 4.755 ± 0.594
2.133ProPhe: 2.133 ± 0.409
5.364ProGly: 5.364 ± 0.745
0.975ProHis: 0.975 ± 0.252
2.682ProIle: 2.682 ± 0.4
2.133ProLys: 2.133 ± 0.339
2.987ProLeu: 2.987 ± 0.365
0.975ProMet: 0.975 ± 0.233
2.743ProAsn: 2.743 ± 0.36
2.012ProPro: 2.012 ± 0.347
2.073ProGln: 2.073 ± 0.393
3.718ProArg: 3.718 ± 0.555
2.438ProSer: 2.438 ± 0.429
4.023ProThr: 4.023 ± 0.511
4.145ProVal: 4.145 ± 0.498
0.731ProTrp: 0.731 ± 0.282
1.463ProTyr: 1.463 ± 0.257
0.0ProXaa: 0.0 ± 0.0
Gln
4.267GlnAla: 4.267 ± 0.659
0.183GlnCys: 0.183 ± 0.108
1.585GlnAsp: 1.585 ± 0.344
1.341GlnGlu: 1.341 ± 0.253
1.158GlnPhe: 1.158 ± 0.204
2.682GlnGly: 2.682 ± 0.385
0.671GlnHis: 0.671 ± 0.165
2.377GlnIle: 2.377 ± 0.379
1.341GlnLys: 1.341 ± 0.263
2.56GlnLeu: 2.56 ± 0.378
0.731GlnMet: 0.731 ± 0.229
0.792GlnAsn: 0.792 ± 0.195
1.646GlnPro: 1.646 ± 0.313
1.768GlnGln: 1.768 ± 0.393
2.621GlnArg: 2.621 ± 0.434
1.89GlnSer: 1.89 ± 0.405
1.524GlnThr: 1.524 ± 0.326
3.17GlnVal: 3.17 ± 0.347
0.792GlnTrp: 0.792 ± 0.267
0.914GlnTyr: 0.914 ± 0.228
0.0GlnXaa: 0.0 ± 0.0
Arg
5.425ArgAla: 5.425 ± 0.543
0.914ArgCys: 0.914 ± 0.343
3.596ArgAsp: 3.596 ± 0.546
4.633ArgGlu: 4.633 ± 0.615
2.438ArgPhe: 2.438 ± 0.443
4.328ArgGly: 4.328 ± 0.462
1.402ArgHis: 1.402 ± 0.305
3.536ArgIle: 3.536 ± 0.516
3.353ArgLys: 3.353 ± 0.432
5.547ArgLeu: 5.547 ± 0.607
2.438ArgMet: 2.438 ± 0.354
2.012ArgAsn: 2.012 ± 0.348
2.56ArgPro: 2.56 ± 0.369
1.89ArgGln: 1.89 ± 0.376
6.035ArgArg: 6.035 ± 0.778
3.596ArgSer: 3.596 ± 0.437
2.804ArgThr: 2.804 ± 0.379
4.389ArgVal: 4.389 ± 0.457
1.463ArgTrp: 1.463 ± 0.289
1.951ArgTyr: 1.951 ± 0.315
0.0ArgXaa: 0.0 ± 0.0
Ser
5.12SerAla: 5.12 ± 0.541
0.731SerCys: 0.731 ± 0.195
4.389SerAsp: 4.389 ± 0.513
4.267SerGlu: 4.267 ± 0.599
2.316SerPhe: 2.316 ± 0.323
5.425SerGly: 5.425 ± 0.65
0.853SerHis: 0.853 ± 0.197
2.621SerIle: 2.621 ± 0.37
2.804SerLys: 2.804 ± 0.468
4.572SerLeu: 4.572 ± 0.629
1.341SerMet: 1.341 ± 0.287
1.585SerAsn: 1.585 ± 0.336
3.292SerPro: 3.292 ± 0.498
2.438SerGln: 2.438 ± 0.346
4.206SerArg: 4.206 ± 0.505
3.779SerSer: 3.779 ± 0.515
3.109SerThr: 3.109 ± 0.388
4.023SerVal: 4.023 ± 0.464
1.28SerTrp: 1.28 ± 0.269
1.097SerTyr: 1.097 ± 0.295
0.0SerXaa: 0.0 ± 0.0
Thr
5.181ThrAla: 5.181 ± 0.511
0.549ThrCys: 0.549 ± 0.183
3.536ThrAsp: 3.536 ± 0.455
3.718ThrGlu: 3.718 ± 0.416
2.255ThrPhe: 2.255 ± 0.297
4.998ThrGly: 4.998 ± 0.685
1.158ThrHis: 1.158 ± 0.306
2.682ThrIle: 2.682 ± 0.34
3.17ThrLys: 3.17 ± 0.411
4.998ThrLeu: 4.998 ± 0.497
1.28ThrMet: 1.28 ± 0.345
1.707ThrAsn: 1.707 ± 0.285
4.145ThrPro: 4.145 ± 0.44
2.621ThrGln: 2.621 ± 0.362
3.048ThrArg: 3.048 ± 0.385
3.596ThrSer: 3.596 ± 0.503
2.865ThrThr: 2.865 ± 0.432
5.059ThrVal: 5.059 ± 0.562
0.914ThrTrp: 0.914 ± 0.227
1.524ThrTyr: 1.524 ± 0.282
0.0ThrXaa: 0.0 ± 0.0
Val
6.644ValAla: 6.644 ± 0.529
0.853ValCys: 0.853 ± 0.278
5.73ValAsp: 5.73 ± 0.512
4.877ValGlu: 4.877 ± 0.517
2.133ValPhe: 2.133 ± 0.394
5.303ValGly: 5.303 ± 0.551
0.975ValHis: 0.975 ± 0.259
3.962ValIle: 3.962 ± 0.517
3.475ValLys: 3.475 ± 0.493
5.547ValLeu: 5.547 ± 0.56
1.89ValMet: 1.89 ± 0.376
2.926ValAsn: 2.926 ± 0.511
3.779ValPro: 3.779 ± 0.473
1.89ValGln: 1.89 ± 0.328
3.901ValArg: 3.901 ± 0.491
4.877ValSer: 4.877 ± 0.5
4.206ValThr: 4.206 ± 0.548
6.035ValVal: 6.035 ± 0.675
1.28ValTrp: 1.28 ± 0.292
1.89ValTyr: 1.89 ± 0.329
0.0ValXaa: 0.0 ± 0.0
Trp
1.402TrpAla: 1.402 ± 0.315
0.305TrpCys: 0.305 ± 0.146
1.219TrpAsp: 1.219 ± 0.251
1.463TrpGlu: 1.463 ± 0.266
0.549TrpPhe: 0.549 ± 0.189
0.975TrpGly: 0.975 ± 0.29
0.549TrpHis: 0.549 ± 0.183
1.097TrpIle: 1.097 ± 0.292
0.914TrpLys: 0.914 ± 0.185
1.524TrpLeu: 1.524 ± 0.306
0.366TrpMet: 0.366 ± 0.142
0.914TrpAsn: 0.914 ± 0.199
0.914TrpPro: 0.914 ± 0.238
1.158TrpGln: 1.158 ± 0.266
0.792TrpArg: 0.792 ± 0.184
1.707TrpSer: 1.707 ± 0.288
1.158TrpThr: 1.158 ± 0.248
1.463TrpVal: 1.463 ± 0.29
0.671TrpTrp: 0.671 ± 0.224
0.549TrpTyr: 0.549 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.133TyrAla: 2.133 ± 0.343
0.305TyrCys: 0.305 ± 0.132
2.255TyrAsp: 2.255 ± 0.338
1.829TyrGlu: 1.829 ± 0.356
0.853TyrPhe: 0.853 ± 0.202
2.255TyrGly: 2.255 ± 0.372
0.366TyrHis: 0.366 ± 0.15
1.036TyrIle: 1.036 ± 0.223
0.671TyrLys: 0.671 ± 0.204
3.17TyrLeu: 3.17 ± 0.449
0.427TyrMet: 0.427 ± 0.143
0.731TyrAsn: 0.731 ± 0.224
1.28TyrPro: 1.28 ± 0.244
1.219TyrGln: 1.219 ± 0.221
1.768TyrArg: 1.768 ± 0.357
1.524TyrSer: 1.524 ± 0.282
2.133TyrThr: 2.133 ± 0.308
1.829TyrVal: 1.829 ± 0.351
0.183TyrTrp: 0.183 ± 0.109
1.036TyrTyr: 1.036 ± 0.294
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (16406 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski